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bethyl recognizing phospho kap1  (Bethyl)


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    Structured Review

    Bethyl bethyl recognizing phospho kap1
    Bethyl Recognizing Phospho Kap1, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kap1/Phospho+KAP-1+(S824)+Antibody/pm41897352-64-71-71
    Average 94 stars, based on 303 article reviews
    bethyl recognizing phospho kap1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Saline:

    Article Title: The Small Molecule SR8278 Inhibits Cell Proliferation Independent of the REV-ERB Nuclear Receptor Proteins in Human Keratinocytes.
    Article Snippet: Blots were stained with 0.5% Ponceau S (Sigma P3504, St. Louis, MO, USA) to ensure equal loading. .. The blots were blocked in 5% non-fat milk (Meijer, Tipp City, OH, USA) in TBST (Tris-buffered saline containing 0.1% Tween-20) and then probed overnight with primary antibodies from Cell Signaling Technology (Danvers, MA, USA) recognizing E2F1 (#3742), RRM2 (#65939), Cyclin E2 (#4132), phospho-CHK1 (Ser345; #2348), phospho-H2AX (Ser1139; #9718), PARP (#9542), or REV-ERBα (#13418), antibodies from Santa Cruz Biotechnology (Dallas, TX, USA) recognizing PCNA (sc-56) or REV-ERBβ (sc-398252), or an antibody from Bethyl recognizing phospho-KAP1 (Ser824; A300-767A). .. After washing with TBST, blots were probed with HRP-coupled anti-rabbit or anti-mouse IgG (Invitrogen) secondary antibodies for one hour at room temperature.

    Article Title: The Small Molecule SR8278 Inhibits Cell Proliferation Independent of the REV-ERB Nuclear Receptor Proteins in Human Keratinocytes
    Article Snippet: Blots were stained with 0.5% Ponceau S (Sigma P3504, St. Louis, MO, USA) to ensure equal loading. .. The blots were blocked in 5% non-fat milk (Meijer, Tipp City, OH, USA) in TBST (Tris-buffered saline containing 0.1% Tween-20) and then probed overnight with primary antibodies from Cell Signaling Technology (Danvers, MA, USA) recognizing E2F1 (#3742), RRM2 (#65939), Cyclin E2 (#4132), phospho-CHK1 (Ser345; #2348), phospho-H2AX (Ser1139; #9718), PARP (#9542), or REV-ERBα (#13418), antibodies from Santa Cruz Biotechnology (Dallas, TX, USA) recognizing PCNA (sc-56) or REV-ERBβ (sc-398252), or an antibody from Bethyl recognizing phospho-KAP1 (Ser824; A300-767A). .. After washing with TBST, blots were probed with HRP-coupled anti-rabbit or anti-mouse IgG (Invitrogen) secondary antibodies for one hour at room temperature.

    Immunostaining:

    Article Title: Expansion of outer cortical CUX2 neurons requires adaptations for DNA repair.
    Article Snippet: .. For the immunostaining: GFP was detected with antibody GFP-1020 (Aves) at 1:1,000 dilution; mouse PAX6 was detected with antibody AB2237 (Millipore) at 1:500 dilution; mouse TBR2 was detected with antibody ab23345 (Abcam) at 1:500 dilution; mouse TBR1 was detected with antibody ab31940 (Abcam) at 1:1,000 dilution; mouse TUJ1 was detected with antibody T2200 (Sigma) at 1:1,000 dilution; mouse SATB2 was detected with antibody ab51502 (Abcam) at 1:500 dilution; mouse CTIP2 was detected with antibody ab18465 (Abcam) at 1:1,000 dilution; mouse CUX1 + CUX2 was detected with antibody ab309139 (Abcam) at 1:500 dilution; mouse calretinin was detected with antibody MAB1568 (Millipore) at 1:500 dilution; mouse parvalbumin was detected with antibody MAB1572 (Millipore) at 1:500 dilution; mouse calbindin was detected with antibody CB38a (Swant) at 1:500 dilution; human PAX6 was detected with antibody 901301 (Biolegend) at 1:500 dilution; mouse γH2A.X was detected with antibody ab2893 (abcam) at 1:500 dilution or 05-636 (Millipore) at 1:500 dilution; mouse CC3 was detected with antibody 9661 (Cell Signaling Technology) at 1:400 dilution; mouse Phospho-KAP-1 (Ser824) was detected with antibody A300-767A (Bethyl Laboratories) at 1:1,000 dilution; mouse p53 was detected with antibody 2524 (Cell Signaling Technology) at 1:500 dilution; mouse PCNA was detected with antibody 2586 (Cell Signaling Technology) at 1:500 dilution; mouse Ki67 was detected with antibody 550609 (BD Biosciences) at 1:500 dilution; mouse 53BP1 was detected with antibody NB100-304 (Novus Biologicals) at 1:500 dilution; mouse DNA-RNA Hybrid S9.6 was detected with antibody ENH001 (Kerafast) at 1:500 dilution; mouse p-ATM(Ser1981) was detected with antibody 05-740 (Millipore Sigma) at 1:500 dilution; mouse PHH3 was detected with Phospho-Histone H3 (Ser10) Antibody 9701 (Cell Signaling Technology at 1:500 dilution; mouse Nestin was detected with antibody MAB353 (Millipore Sigma) at 1:500 dilution; and mouse SOX2 was detected with antibody ab92494 (Abcam) at 1:500 dilution. ..

    Western Blot:

    Article Title: Targeting EphA2 under DNA damage causes mitotic bypass via p21 induction
    Article Snippet: For Ca Ski cells, transfection was performed using PEI MAX (24765, Polysciences), and cells were selected with 2 μg/ml blasticidin S for 14 days. .. Primary antibodies used for immunoblotting (IB), immunofluorescence (IF) or flow cytometry were as follows: mouse monoclonal anti-Chk1 (IB, 1:1000; G-4, sc-8408, Santa Cruz Biotechnology), anti-γ-tubulin (IF, 1:250; GTU-88, T6557, Merck), anti-p21 (IB, 1:1000, DCS60, 2946, Cell Signaling Technology), anti-p53 (IB, 1:1000; DO-1, sc-126, Santa Cruz Biotechnology), and anti-phospho-histone H3 (IF, 1:400; 6G3, 9706, Cell Signaling Technology) antibodies; rabbit monoclonal anti-EphA2 (IB, 1:1000; 6997S, Cell Signaling Technology), anti-phospho-Chk1 (Ser345, IB, 1:1000; 133D3, #2348, Cell Signaling Technology), and anti-phospho-EphA2 (Ser897, IB, 1:1000; D9A1, 6347, Cell Signaling Technology) antibodies; rabbit polyclonal anti-cyclin B1 (IB, 1:3000; IF and flow cytometry, 1:250; H-433, sc-752, Santa Cruz Biotechnology), anti-phospho-histone H2A.X (γH2AX, IB, 1:500; 2577S, Cell Signaling Technology), and anti-phospho KAP1 (Ser824, IB, 1:1000; A300–767A, Bethyl Laboratories, Montgomery) antibodies; and rat monoclonal anti-α-tubulin (IB, 1:4000; IF, 1:800; MCA78 G, Bio-Rad) antibody. .. For immunoblotting analysis, horseradish peroxidase-conjugated donkey anti-mouse IgG (1:8000; 715–035–151), donkey anti-rabbit IgG (1:8000; 711–035–152), and donkey anti-rat IgG (1:8000–10000; 712–035–153) antibodies were purchased from Jackson ImmunoResearch Laboratories and used as secondary antibodies.

    Immunofluorescence:

    Article Title: Targeting EphA2 under DNA damage causes mitotic bypass via p21 induction
    Article Snippet: For Ca Ski cells, transfection was performed using PEI MAX (24765, Polysciences), and cells were selected with 2 μg/ml blasticidin S for 14 days. .. Primary antibodies used for immunoblotting (IB), immunofluorescence (IF) or flow cytometry were as follows: mouse monoclonal anti-Chk1 (IB, 1:1000; G-4, sc-8408, Santa Cruz Biotechnology), anti-γ-tubulin (IF, 1:250; GTU-88, T6557, Merck), anti-p21 (IB, 1:1000, DCS60, 2946, Cell Signaling Technology), anti-p53 (IB, 1:1000; DO-1, sc-126, Santa Cruz Biotechnology), and anti-phospho-histone H3 (IF, 1:400; 6G3, 9706, Cell Signaling Technology) antibodies; rabbit monoclonal anti-EphA2 (IB, 1:1000; 6997S, Cell Signaling Technology), anti-phospho-Chk1 (Ser345, IB, 1:1000; 133D3, #2348, Cell Signaling Technology), and anti-phospho-EphA2 (Ser897, IB, 1:1000; D9A1, 6347, Cell Signaling Technology) antibodies; rabbit polyclonal anti-cyclin B1 (IB, 1:3000; IF and flow cytometry, 1:250; H-433, sc-752, Santa Cruz Biotechnology), anti-phospho-histone H2A.X (γH2AX, IB, 1:500; 2577S, Cell Signaling Technology), and anti-phospho KAP1 (Ser824, IB, 1:1000; A300–767A, Bethyl Laboratories, Montgomery) antibodies; and rat monoclonal anti-α-tubulin (IB, 1:4000; IF, 1:800; MCA78 G, Bio-Rad) antibody. .. For immunoblotting analysis, horseradish peroxidase-conjugated donkey anti-mouse IgG (1:8000; 715–035–151), donkey anti-rabbit IgG (1:8000; 711–035–152), and donkey anti-rat IgG (1:8000–10000; 712–035–153) antibodies were purchased from Jackson ImmunoResearch Laboratories and used as secondary antibodies.

    Flow Cytometry:

    Article Title: Targeting EphA2 under DNA damage causes mitotic bypass via p21 induction
    Article Snippet: For Ca Ski cells, transfection was performed using PEI MAX (24765, Polysciences), and cells were selected with 2 μg/ml blasticidin S for 14 days. .. Primary antibodies used for immunoblotting (IB), immunofluorescence (IF) or flow cytometry were as follows: mouse monoclonal anti-Chk1 (IB, 1:1000; G-4, sc-8408, Santa Cruz Biotechnology), anti-γ-tubulin (IF, 1:250; GTU-88, T6557, Merck), anti-p21 (IB, 1:1000, DCS60, 2946, Cell Signaling Technology), anti-p53 (IB, 1:1000; DO-1, sc-126, Santa Cruz Biotechnology), and anti-phospho-histone H3 (IF, 1:400; 6G3, 9706, Cell Signaling Technology) antibodies; rabbit monoclonal anti-EphA2 (IB, 1:1000; 6997S, Cell Signaling Technology), anti-phospho-Chk1 (Ser345, IB, 1:1000; 133D3, #2348, Cell Signaling Technology), and anti-phospho-EphA2 (Ser897, IB, 1:1000; D9A1, 6347, Cell Signaling Technology) antibodies; rabbit polyclonal anti-cyclin B1 (IB, 1:3000; IF and flow cytometry, 1:250; H-433, sc-752, Santa Cruz Biotechnology), anti-phospho-histone H2A.X (γH2AX, IB, 1:500; 2577S, Cell Signaling Technology), and anti-phospho KAP1 (Ser824, IB, 1:1000; A300–767A, Bethyl Laboratories, Montgomery) antibodies; and rat monoclonal anti-α-tubulin (IB, 1:4000; IF, 1:800; MCA78 G, Bio-Rad) antibody. .. For immunoblotting analysis, horseradish peroxidase-conjugated donkey anti-mouse IgG (1:8000; 715–035–151), donkey anti-rabbit IgG (1:8000; 711–035–152), and donkey anti-rat IgG (1:8000–10000; 712–035–153) antibodies were purchased from Jackson ImmunoResearch Laboratories and used as secondary antibodies.



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