Review




Structured Review

Nikon jobs module
Image acquisition. (A) In each well of a 96-well plate, a stack of 10 images separated by 1 μM in the z-axis was collected from five regions of interests (ROIs). The perfect focus system (PFS) uses an IR laser to locate the bottom of the plate so that image acquisition begins at a precise z-offset from the bottom of the well. Flow chart shows sequence of alignment, movement and acquisition (scan) for automated microscope controlled by the <t>JOBS</t> module in <t>Elements</t> <t>software.</t> (B) Rat hippocampal cultures in 96-well plates were transduced using a bicistronic HdAd virus with a hSyn promoter driving independent expression of postsynaptic density 95-enhanced green fluorescent protein (PSD95-eGFP) (green) and mCherry (red). Images were collected with laser scanning confocal microscopy or with wide-field microscopy as described in “Materials and Methods” section. Maximum intensity projections (MIPs; shown) for the confocal and wide-field (after deconvolution) images are uploaded for subsequent image processing. Scale bar: 10 μM.
Jobs Module, supplied by Nikon, used in various techniques. Bioz Stars score: 95/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jobs+module/Maximum+Magnification+Module/pmc06811609-87-26-29
Average 95 stars, based on 311 article reviews
jobs module - by Bioz Stars, 2026-10
95/100 stars

Images

1) Product Images from "Automated Live-Cell Imaging of Synapses in Rat and Human Neuronal Cultures"

Article Title: Automated Live-Cell Imaging of Synapses in Rat and Human Neuronal Cultures

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2019.00467

Image acquisition. (A) In each well of a 96-well plate, a stack of 10 images separated by 1 μM in the z-axis was collected from five regions of interests (ROIs). The perfect focus system (PFS) uses an IR laser to locate the bottom of the plate so that image acquisition begins at a precise z-offset from the bottom of the well. Flow chart shows sequence of alignment, movement and acquisition (scan) for automated microscope controlled by the JOBS module in Elements software. (B) Rat hippocampal cultures in 96-well plates were transduced using a bicistronic HdAd virus with a hSyn promoter driving independent expression of postsynaptic density 95-enhanced green fluorescent protein (PSD95-eGFP) (green) and mCherry (red). Images were collected with laser scanning confocal microscopy or with wide-field microscopy as described in “Materials and Methods” section. Maximum intensity projections (MIPs; shown) for the confocal and wide-field (after deconvolution) images are uploaded for subsequent image processing. Scale bar: 10 μM.
Figure Legend Snippet: Image acquisition. (A) In each well of a 96-well plate, a stack of 10 images separated by 1 μM in the z-axis was collected from five regions of interests (ROIs). The perfect focus system (PFS) uses an IR laser to locate the bottom of the plate so that image acquisition begins at a precise z-offset from the bottom of the well. Flow chart shows sequence of alignment, movement and acquisition (scan) for automated microscope controlled by the JOBS module in Elements software. (B) Rat hippocampal cultures in 96-well plates were transduced using a bicistronic HdAd virus with a hSyn promoter driving independent expression of postsynaptic density 95-enhanced green fluorescent protein (PSD95-eGFP) (green) and mCherry (red). Images were collected with laser scanning confocal microscopy or with wide-field microscopy as described in “Materials and Methods” section. Maximum intensity projections (MIPs; shown) for the confocal and wide-field (after deconvolution) images are uploaded for subsequent image processing. Scale bar: 10 μM.

Techniques Used: Sequencing, Microscopy, Software, Virus, Expressing, Confocal Microscopy

Related Articles

Microscopy:

Article Title: Methods for multiplex imaging using labeled nucleic acid imaging agents
Article Snippet: .. Samples were loaded onto an inverted Nikon Eclipse Ti microscope (Nikon Instruments) with a fluorescence module and an Andor Zyla sCMOS camera. ..

Article Title: Methods for multiplex imaging using labeled nucleic acid imaging agents
Article Snippet: .. Samples were imaged using an inverted Nikon Eclipse Ti microscope (Nikon Instruments) with a fluorescence module and an Andor Zyla sCMOS camera. ..

Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
Article Snippet: Aims: In Alzheimer's disease (AD), the pathological accumulation of tau in synapses contributes to synapse dysfunction and loss.. However, the small and complex structure of synapses limits the investigation when using conventional techniques.. In this work, we describe the combination of array tomography (AT) with twocolour direct stochastic optical reconstruction microscopy (dSTORM) to enhance lateral resolution for resolving synaptic terminals in human postmortem brain.

Fluorescence:

Article Title: Methods for multiplex imaging using labeled nucleic acid imaging agents
Article Snippet: .. Samples were loaded onto an inverted Nikon Eclipse Ti microscope (Nikon Instruments) with a fluorescence module and an Andor Zyla sCMOS camera. ..

Article Title: Methods for multiplex imaging using labeled nucleic acid imaging agents
Article Snippet: .. Samples were imaged using an inverted Nikon Eclipse Ti microscope (Nikon Instruments) with a fluorescence module and an Andor Zyla sCMOS camera. ..

Software:

Article Title: Amylin exacerbates tau pathology in the visual cortex of diabetic mice by impairing lysosomal activity
Article Snippet: .. High-magnification z-stack images 265 were converted to maximum intensity 2D projections and deconvolved in NIS-Elements 266 software (Nikon) 52. ..

Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
Article Snippet: Aims: In Alzheimer's disease (AD), the pathological accumulation of tau in synapses contributes to synapse dysfunction and loss.. However, the small and complex structure of synapses limits the investigation when using conventional techniques.. In this work, we describe the combination of array tomography (AT) with twocolour direct stochastic optical reconstruction microscopy (dSTORM) to enhance lateral resolution for resolving synaptic terminals in human postmortem brain.

other:

Article Title: Synergistic Effects of Polycationic and Polyfluorinated Functionalities for Efficient Intracellular Protein Delivery.
Article Snippet: Intracellular protein therapy is a promising strategy in biologics, including vaccine development, gene editing, and cancer therapeutics.. However, protein-based drug delivery remains a significant challenge, particularly in penetrating cell barriers to reach intracellular targets.. Inspired by transport adjuvants, we designed a series of polymeric vectors to achieve efficient functional protein trafficking with low cytotoxicity.

Imaging:

Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
Article Snippet: Aims: In Alzheimer's disease (AD), the pathological accumulation of tau in synapses contributes to synapse dysfunction and loss.. However, the small and complex structure of synapses limits the investigation when using conventional techniques.. In this work, we describe the combination of array tomography (AT) with twocolour direct stochastic optical reconstruction microscopy (dSTORM) to enhance lateral resolution for resolving synaptic terminals in human postmortem brain.

Immunofluorescence:

Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
Article Snippet: Aims: In Alzheimer's disease (AD), the pathological accumulation of tau in synapses contributes to synapse dysfunction and loss.. However, the small and complex structure of synapses limits the investigation when using conventional techniques.. In this work, we describe the combination of array tomography (AT) with twocolour direct stochastic optical reconstruction microscopy (dSTORM) to enhance lateral resolution for resolving synaptic terminals in human postmortem brain.

Activation Assay:

Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
Article Snippet: Aims: In Alzheimer's disease (AD), the pathological accumulation of tau in synapses contributes to synapse dysfunction and loss.. However, the small and complex structure of synapses limits the investigation when using conventional techniques.. In this work, we describe the combination of array tomography (AT) with twocolour direct stochastic optical reconstruction microscopy (dSTORM) to enhance lateral resolution for resolving synaptic terminals in human postmortem brain.



Similar Products

99
Nikon nis elements jobs modules
Nis Elements Jobs Modules, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jobs+module/NIS-Elements/pm41840126-440-39-43
Average 99 stars, based on 1 article reviews
nis elements jobs modules - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier

99
Nikon nikon nis elements jobs module
Nikon Nis Elements Jobs Module, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jobs+module/NIS-Elements/bio_rxiv__2025__11__01__685922-304-35-35
Average 99 stars, based on 1 article reviews
nikon nis elements jobs module - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier

99
Nikon nis elements advanced research software jobs module
Nis Elements Advanced Research Software Jobs Module, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jobs+module/NIS-Elements/bio_rxiv__2025__09__25__678644-187-10-17
Average 99 stars, based on 1 article reviews
nis elements advanced research software jobs module - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier

90
Nikon jobs module of nikon nis elements software
Jobs Module Of Nikon Nis Elements Software, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jobs+module/pmc12146582-96-10-13
Average 90 stars, based on 1 article reviews
jobs module of nikon nis elements software - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Nikon jobs module
Jobs Module, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jobs+module/bio_rxiv__2025__05__28__656493-347-11-14
Average 90 stars, based on 1 article reviews
jobs module - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results