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cd63  (Cell Signaling Technology Inc)


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Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc cd63
    Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/java+runtime+engine+(jre)/BAP1+Rabbit+mAb/pm37715968-68-7-5
    Average 94 stars, based on 21 article reviews
    cd63 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Unleashed condensation by recurrent mutations of an epigenetic regulator promotes cancer
    Article Snippet: To determine partition coefficient, the fluorescence intensity inside GFP foci (I in ) and the intensity outside the foci (I out ) was determined by ZEN2.6 software (Carl Zeiss), and partition coefficient was calculated as I in /I out . .. Antibodies for the following proteins were used: BAP1 (Cell Signaling, #13187, RRID: AB_2798143), ASXL1 N-terminal (Abcam, ab228009, No RRID but used in literature , , and its staining pattern completely overlaps with GFP signal in our FmEGFP-ASXL1 Y591X cells), ASXL1 C-terminal (Abcam, ab50817, RRID:AB_867743), BRD4 (Abcam, ab128874, RRID:AB_11145462), Ser2 phosphorylated RNA-Pol II (Abcam, ab193468, RRID:AB_2905557), HP1α (Cell Signaling, #2616, RRID:AB_2070987), H2AK119ub (Cell Signaling, #8240, RRID:AB_10891618), GAPDH (Millipore # MAB374, RRID:AB_2107445, mouse, 1:5000 for immunoblotting), FLAG (Sigma # A2220, RRID: AB_10063035), HA (Cell signaling, #3724, RRID: AB_1549585, Rabbit, 1:1000 for immunoblotting). .. We also used AlexaFluor 555 conjugated goat anti-rabbit IgG (Thermo Fisher, A-21428, RRID:AB_141784), and Alexa Fluor 488 conjugated goat anti-mouse IgG (Thermo Fisher, A-11001, RRID:AB_2534069).

    Western Blot:

    Article Title: Unleashed condensation by recurrent mutations of an epigenetic regulator promotes cancer
    Article Snippet: To determine partition coefficient, the fluorescence intensity inside GFP foci (I in ) and the intensity outside the foci (I out ) was determined by ZEN2.6 software (Carl Zeiss), and partition coefficient was calculated as I in /I out . .. Antibodies for the following proteins were used: BAP1 (Cell Signaling, #13187, RRID: AB_2798143), ASXL1 N-terminal (Abcam, ab228009, No RRID but used in literature , , and its staining pattern completely overlaps with GFP signal in our FmEGFP-ASXL1 Y591X cells), ASXL1 C-terminal (Abcam, ab50817, RRID:AB_867743), BRD4 (Abcam, ab128874, RRID:AB_11145462), Ser2 phosphorylated RNA-Pol II (Abcam, ab193468, RRID:AB_2905557), HP1α (Cell Signaling, #2616, RRID:AB_2070987), H2AK119ub (Cell Signaling, #8240, RRID:AB_10891618), GAPDH (Millipore # MAB374, RRID:AB_2107445, mouse, 1:5000 for immunoblotting), FLAG (Sigma # A2220, RRID: AB_10063035), HA (Cell signaling, #3724, RRID: AB_1549585, Rabbit, 1:1000 for immunoblotting). .. We also used AlexaFluor 555 conjugated goat anti-rabbit IgG (Thermo Fisher, A-21428, RRID:AB_141784), and Alexa Fluor 488 conjugated goat anti-mouse IgG (Thermo Fisher, A-11001, RRID:AB_2534069).

    Immunoprecipitation:

    Article Title: Loss of histone deubiquitinase Bap1 triggers anti-tumor immunity.
    Article Snippet: For immunoprecipitation with Elf4, lysates were incubated with prewashed Pierce c-Myc magnetic beads (Thermo Fisher Scientific, 88842) for 30 min at room temperature, the beads were washed with 5× TBST buffer (125mM Tris, 0.75 M NaCl, 0.25% Tween-20 Detergent) 3 times and ultrapure water once and boiled with SDS sample buffer. .. For immunoprecipitation with Bap1, lysates were incubated overnight with anti-Bap1 antibody (CST, 13187), then Dynabeads Protein G (Invitrogen, 10003D) was added and incubated for 1 h, the beads were washed with PBS 3 times and boiled with SDS sample buffer. ..

    Article Title: Loss of histone deubiquitinase Bap1 triggers anti-tumor immunity
    Article Snippet: For immunoprecipitation with Elf4, lysates were incubated with prewashed Pierce c-Myc magnetic beads (Thermo Fisher Scientific, 88842) for 30 min at room temperature, the beads were washed with 5× TBST buffer (125mM Tris, 0.75 M NaCl, 0.25% Tween-20 Detergent) 3 times and ultrapure water once and boiled with SDS sample buffer. .. For immunoprecipitation with Bap1, lysates were incubated overnight with anti-Bap1 antibody (CST, 13187), then Dynabeads Protein G (Invitrogen, 10003D) was added and incubated for 1 h, the beads were washed with PBS 3 times and boiled with SDS sample buffer. ..

    Incubation:

    Article Title: Loss of histone deubiquitinase Bap1 triggers anti-tumor immunity.
    Article Snippet: For immunoprecipitation with Elf4, lysates were incubated with prewashed Pierce c-Myc magnetic beads (Thermo Fisher Scientific, 88842) for 30 min at room temperature, the beads were washed with 5× TBST buffer (125mM Tris, 0.75 M NaCl, 0.25% Tween-20 Detergent) 3 times and ultrapure water once and boiled with SDS sample buffer. .. For immunoprecipitation with Bap1, lysates were incubated overnight with anti-Bap1 antibody (CST, 13187), then Dynabeads Protein G (Invitrogen, 10003D) was added and incubated for 1 h, the beads were washed with PBS 3 times and boiled with SDS sample buffer. ..

    Article Title: Loss of histone deubiquitinase Bap1 triggers anti-tumor immunity
    Article Snippet: For immunoprecipitation with Elf4, lysates were incubated with prewashed Pierce c-Myc magnetic beads (Thermo Fisher Scientific, 88842) for 30 min at room temperature, the beads were washed with 5× TBST buffer (125mM Tris, 0.75 M NaCl, 0.25% Tween-20 Detergent) 3 times and ultrapure water once and boiled with SDS sample buffer. .. For immunoprecipitation with Bap1, lysates were incubated overnight with anti-Bap1 antibody (CST, 13187), then Dynabeads Protein G (Invitrogen, 10003D) was added and incubated for 1 h, the beads were washed with PBS 3 times and boiled with SDS sample buffer. ..

    Recombinant:

    Article Title: P21 resists ferroptosis in osteoarthritic chondrocytes by regulating GPX4 protein stability.
    Article Snippet: .. The commercial antibodies and reagents used in this study were described as follow: Recombinant mouse IL-1β (#401-ML, R&D systems), DMSO (HY–Y0320, MCE), Erastin (S7242, Selleck), MG132 (S2619, Selleck), Fer-1 (S7243, Selleck), N-Ethylmaleimide (S3692, Selleck), E64D (S7393, Selleck), RSL3 (HY-100218A, MCE), PS341 (HY- 10227, MCE), CHX (HY-12320, MCE), FAC (1185-57-5, Sigma–Aldrich), TBHQ (HY-100489, MCE), GPX4 (ab125066, Abcam), GPX4 (67763-1- Ig, Proteintech), SLC7A11 (ab175186, Abcam), Ubiquitin linkagespecific K48 (ab140601, Abcam), P21 (28248-1-AP, Proteintech), P21 (ab188224 and ab109520, Abcam), P53 (60283-2-Ig, Proteintech), NRF2 (16396-1-AP, Proteintech), KEAP1 (10503-2-AP, Proteintech), Col2a1 (28459-1-AP, Proteintech), HO-1 (10701-1-AP, Proteintech), MMP13 (18165-1-AP, Proteintech), β-actin (66009-1-Ig, Proteintech), GAPDH (60004-1-Ig, Proteintech), MYC (60003-2-Ig, Proteintech), SHARPIN (14626-1-AP, Proteintech), Ubiquitin (#43124S, CST), NQO-1 (#3187S, CST), iNOS (#13120S, CST), Ub-M1 (PA5-120624, ThermoFisher Scientific), HOIP (A14139, ABclonal). ..

    Ubiquitin Proteomics:

    Article Title: P21 resists ferroptosis in osteoarthritic chondrocytes by regulating GPX4 protein stability.
    Article Snippet: .. The commercial antibodies and reagents used in this study were described as follow: Recombinant mouse IL-1β (#401-ML, R&D systems), DMSO (HY–Y0320, MCE), Erastin (S7242, Selleck), MG132 (S2619, Selleck), Fer-1 (S7243, Selleck), N-Ethylmaleimide (S3692, Selleck), E64D (S7393, Selleck), RSL3 (HY-100218A, MCE), PS341 (HY- 10227, MCE), CHX (HY-12320, MCE), FAC (1185-57-5, Sigma–Aldrich), TBHQ (HY-100489, MCE), GPX4 (ab125066, Abcam), GPX4 (67763-1- Ig, Proteintech), SLC7A11 (ab175186, Abcam), Ubiquitin linkagespecific K48 (ab140601, Abcam), P21 (28248-1-AP, Proteintech), P21 (ab188224 and ab109520, Abcam), P53 (60283-2-Ig, Proteintech), NRF2 (16396-1-AP, Proteintech), KEAP1 (10503-2-AP, Proteintech), Col2a1 (28459-1-AP, Proteintech), HO-1 (10701-1-AP, Proteintech), MMP13 (18165-1-AP, Proteintech), β-actin (66009-1-Ig, Proteintech), GAPDH (60004-1-Ig, Proteintech), MYC (60003-2-Ig, Proteintech), SHARPIN (14626-1-AP, Proteintech), Ubiquitin (#43124S, CST), NQO-1 (#3187S, CST), iNOS (#13120S, CST), Ub-M1 (PA5-120624, ThermoFisher Scientific), HOIP (A14139, ABclonal). ..



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