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BGI Shenzhen itraq-based proteomic analysis
Itraq Based Proteomic Analysis, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/itraq-based+proteomics+analysis/itraq+analysis/pmc09740226-343-0-11
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itraq-based proteomic analysis - by Bioz Stars, 2026-11
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Multiplex sample analysis:

Article Title: Robust expression of p27Kip1 induced by viral infection is critical for antiviral innate immunity.
Article Snippet: Influenza A virus (IAV) infection regulates the expression of numerous host genes.. However, the precise mechanism underlying implication of these genes in IAV pathogenesis remains largely unknown.. Here, we employed iTRAQ to identify host proteins regulated by IAV infection. iTRAQ analysis of mouse lungs infected or uninfected with IAV showed a total of 167 differentially upregulated proteins in response to the viral infection.

Article Title: Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles.
Article Snippet: Zinc oxide nanoparticles (ZnO NPs) are promising candidates as animal diet additive.. However, several studies have reported that ZnO NPs cause adverse effects on organisms.. Hen egg yolk proteins play vital roles during embryonic development.

Article Title: Integrated transmission electron microscopy and proteomic analyses reveal the cytoarchitectural response to cucumber mosaic virus infection in tobacco.
Article Snippet: Cucumber mosaic virus (CMV) causes huge economic losses to agriculture every year; thus, understanding the mechanism of plant resistance to CMV is imperative.. In this study, an integrated analysis of transmission electron microscopy (TEM) observations and proteomic results was used to identify cytoarchitectural differences in Nicotiana tabacum cv.. NC82 (susceptible) and cv.

Article Title: Cross-Stress Adaptation in a Piezophilic and Hyperthermophilic Archaeon From Deep Sea Hydrothermal Vent
Article Snippet: .. iTRAQ analysis was performed at BGI (Shenzhen, China). .. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD018974 (for pH data), PXD018991 (for temperature data), PXD018994 (for NaCl data), and PXD019007 (for HP data) ( ).

Article Title: Hypoglycemic mechanisms of Ganoderma lucidum polysaccharides F31 in db/db mice via RNA-seq and iTRAQ.
Article Snippet: Our team has previously demonstrated that Ganoderma lucidum polysaccharides F31 have hypoglycemic effects on diabetic mice.. This study provides insight into the system-level hypoglycemic mechanisms of F31 by the integrative analysis of transcriptomics and proteomics data.. To explore the omics perspective for the mechanisms of action, the protein and gene expression in the liver from the normal control (NC), diabetic db/db control mice (DC) and F31-treated db/db mice (F31) were analyzed by iTRAQ and RNA-Seq.

Article Title: Integrative Omics Analysis of Three Oil Palm Varieties Reveals (Tanzania × Ekona) TE as a Cold-Resistant Variety in Response to Low-Temperature Stress
Article Snippet: .. iTRAQ-based proteomic analysis for oil palm leaves was carried out at (BGI) Shenzhen, China. ..

Article Title: Effects of light intensity on metabolism of light-harvesting pigment and photosynthetic system in Camellia sinensis L. cultivar ‘Huangjinya’
Article Snippet: ‘Huangjinya’ (HJY) is a light-sensitive chlorotic tea cultivar (Camellia sinensis L.) that grows chlorotic shoots under sun-light conditions but its chlorotic shoots turn green under weak light conditions.. However, the mechanisms responsible for light intensity-induced changes in leaf color remain unclear.. In this study, we compared HJY plants with chlorotic leaves exposed to sun-light (Y) and HJY plants with green leaves exposed to both sun and shade (Y+S) on the basis of metabolite profiles, expression of proteins and genes in the pigment metabolic pathway and photosynthetic system.

Article Title: Integration of proteomic and transcriptomic profiles reveals multiple levels of genetic regulation of salt tolerance in cotton
Article Snippet: The total protein supernatants of 18 samples (i.e., 2 genotypes × 3 time points × 3 replicates) were transferred to a new tube and quantified using a 2-D Quant Kit (General Electric Company, USA). .. Three biological replicates and three technical replicates were performed to verify the protein quality and concentration. iTRAQ analysis was performed by BGI (Shenzhen, China). ..

Bradford Protein Assay:

Article Title: Integrated transmission electron microscopy and proteomic analyses reveal the cytoarchitectural response to cucumber mosaic virus infection in tobacco.
Article Snippet: Cucumber mosaic virus (CMV) causes huge economic losses to agriculture every year; thus, understanding the mechanism of plant resistance to CMV is imperative.. In this study, an integrated analysis of transmission electron microscopy (TEM) observations and proteomic results was used to identify cytoarchitectural differences in Nicotiana tabacum cv.. NC82 (susceptible) and cv.

SDS Page:

Article Title: Integrated transmission electron microscopy and proteomic analyses reveal the cytoarchitectural response to cucumber mosaic virus infection in tobacco.
Article Snippet: Cucumber mosaic virus (CMV) causes huge economic losses to agriculture every year; thus, understanding the mechanism of plant resistance to CMV is imperative.. In this study, an integrated analysis of transmission electron microscopy (TEM) observations and proteomic results was used to identify cytoarchitectural differences in Nicotiana tabacum cv.. NC82 (susceptible) and cv.

Protein Concentration:

Article Title: Hypoglycemic mechanisms of Ganoderma lucidum polysaccharides F31 in db/db mice via RNA-seq and iTRAQ.
Article Snippet: Our team has previously demonstrated that Ganoderma lucidum polysaccharides F31 have hypoglycemic effects on diabetic mice.. This study provides insight into the system-level hypoglycemic mechanisms of F31 by the integrative analysis of transcriptomics and proteomics data.. To explore the omics perspective for the mechanisms of action, the protein and gene expression in the liver from the normal control (NC), diabetic db/db control mice (DC) and F31-treated db/db mice (F31) were analyzed by iTRAQ and RNA-Seq.

Concentration Assay:

Article Title: Integration of proteomic and transcriptomic profiles reveals multiple levels of genetic regulation of salt tolerance in cotton
Article Snippet: The total protein supernatants of 18 samples (i.e., 2 genotypes × 3 time points × 3 replicates) were transferred to a new tube and quantified using a 2-D Quant Kit (General Electric Company, USA). .. Three biological replicates and three technical replicates were performed to verify the protein quality and concentration. iTRAQ analysis was performed by BGI (Shenzhen, China). ..



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Genechem itraq-based proteomics analysis
(A,B) iTRAQ-based <t>proteomics</t> analysis. (A) Apoptosis promotors Casp9 , Casp8 , Bak1, and Dap were significantly down-regulated by YKL-40, while the apoptosis inhibitor Aven was significantly up-regulated. (B) Volcano Plot . Molecules down-regulated by YKL-40 are presented in the upper left area (green down arrow) while those up-regulated are shown in the upper right area (red up arrow), and those involved in apoptosis regulation are marked with up/down solid arrows. The most significant fold change ( FC ) on the expression level of these molecules was caused by Casp9 ( FC = 0.6411), followed by Aven ( FC = 1.3006). (C,D) Caspase-9 expression levels detected in BMDM and aorta tissues of Ldlr −/- mice. YKL-40 significantly down-regulated the activation level of caspase-9. (E) In RAW264.7, which was upregulated by Ykl-40 , the activation level of caspase-9 was significantly lower than that in control group ( p = 0.0054) while the expression level of Aven was significantly higher than in controls ( p = 0.0031). There was no significant difference in caspase-9 activation and Aven expression level between the Ykl40 downregulated group and normal controls ( p > 0.05). (F) Genetic expression relative fold changes of Casp9 , Dap , Aven and Bak1. No significant difference was indicated on Casp9 in BMDM after being treated by YKL-40 recombinant protein, but Aven was significantly up-regulated. (G) Casp9 , Aven expression levels were significantly upregulated in Ykl40 upregulated RAW264.7 ( p = 0.0154, p < 0.0001) and downregulated in the Ykl40 downregulated group ( p = 0.0039, p = 0.0037).
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(A,B) iTRAQ-based proteomics analysis. (A) Apoptosis promotors Casp9 , Casp8 , Bak1, and Dap were significantly down-regulated by YKL-40, while the apoptosis inhibitor Aven was significantly up-regulated. (B) Volcano Plot . Molecules down-regulated by YKL-40 are presented in the upper left area (green down arrow) while those up-regulated are shown in the upper right area (red up arrow), and those involved in apoptosis regulation are marked with up/down solid arrows. The most significant fold change ( FC ) on the expression level of these molecules was caused by Casp9 ( FC = 0.6411), followed by Aven ( FC = 1.3006). (C,D) Caspase-9 expression levels detected in BMDM and aorta tissues of Ldlr −/- mice. YKL-40 significantly down-regulated the activation level of caspase-9. (E) In RAW264.7, which was upregulated by Ykl-40 , the activation level of caspase-9 was significantly lower than that in control group ( p = 0.0054) while the expression level of Aven was significantly higher than in controls ( p = 0.0031). There was no significant difference in caspase-9 activation and Aven expression level between the Ykl40 downregulated group and normal controls ( p > 0.05). (F) Genetic expression relative fold changes of Casp9 , Dap , Aven and Bak1. No significant difference was indicated on Casp9 in BMDM after being treated by YKL-40 recombinant protein, but Aven was significantly up-regulated. (G) Casp9 , Aven expression levels were significantly upregulated in Ykl40 upregulated RAW264.7 ( p = 0.0154, p < 0.0001) and downregulated in the Ykl40 downregulated group ( p = 0.0039, p = 0.0037).

Journal: Frontiers in Cell and Developmental Biology

Article Title: YKL-40 Aggravates Early-Stage Atherosclerosis by Inhibiting Macrophage Apoptosis in an Aven-dependent Way

doi: 10.3389/fcell.2021.752773

Figure Lengend Snippet: (A,B) iTRAQ-based proteomics analysis. (A) Apoptosis promotors Casp9 , Casp8 , Bak1, and Dap were significantly down-regulated by YKL-40, while the apoptosis inhibitor Aven was significantly up-regulated. (B) Volcano Plot . Molecules down-regulated by YKL-40 are presented in the upper left area (green down arrow) while those up-regulated are shown in the upper right area (red up arrow), and those involved in apoptosis regulation are marked with up/down solid arrows. The most significant fold change ( FC ) on the expression level of these molecules was caused by Casp9 ( FC = 0.6411), followed by Aven ( FC = 1.3006). (C,D) Caspase-9 expression levels detected in BMDM and aorta tissues of Ldlr −/- mice. YKL-40 significantly down-regulated the activation level of caspase-9. (E) In RAW264.7, which was upregulated by Ykl-40 , the activation level of caspase-9 was significantly lower than that in control group ( p = 0.0054) while the expression level of Aven was significantly higher than in controls ( p = 0.0031). There was no significant difference in caspase-9 activation and Aven expression level between the Ykl40 downregulated group and normal controls ( p > 0.05). (F) Genetic expression relative fold changes of Casp9 , Dap , Aven and Bak1. No significant difference was indicated on Casp9 in BMDM after being treated by YKL-40 recombinant protein, but Aven was significantly up-regulated. (G) Casp9 , Aven expression levels were significantly upregulated in Ykl40 upregulated RAW264.7 ( p = 0.0154, p < 0.0001) and downregulated in the Ykl40 downregulated group ( p = 0.0039, p = 0.0037).

Article Snippet: iTRAQ-based proteomics analysis (Genechem Co.,Ltd., Shanghai, China) were performed to screen out the potential downstream target molecules of YKL-40.

Techniques: Expressing, Activation Assay, Recombinant