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isothermal titration calorimetry itc buffer  (Malvern Panalytical)


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    Malvern Panalytical isothermal titration calorimetry itc buffer
    Isothermal Titration Calorimetry Itc Buffer, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/itc+buffer/pm33461211-361-18-55
    Average 86 stars, based on 1 article reviews
    isothermal titration calorimetry itc buffer - by Bioz Stars, 2026-10
    86/100 stars

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    Mutagenesis:

    Article Title: The Card1 nuclease provides defence during type III CRISPR immunity.
    Article Snippet: The UV absorbance data recorded at 260 nm were used, and final graphs are represented using GraphPad Prism v.8.0. .. Wild-type and mutant Card1 proteins were diluted to the final concentrations of 11 to 41 μM in the isothermal titration calorimetry (ITC) buffer composed of 20 mM HEPES, pH 7.5, 150 mM NaCl, 0.5% β-mercaptoethanol, and were titrated against 150 to 500 μM cA4 or cA6 in the same buffer at 20 °C by using MicroCal PEAQ-ITC range (Malvern Panalytical and the High Throughput and Sequencing Center of Rockefeller University). ..

    Isothermal Titration Calorimetry:

    Article Title: The Card1 nuclease provides defence during type III CRISPR immunity.
    Article Snippet: The UV absorbance data recorded at 260 nm were used, and final graphs are represented using GraphPad Prism v.8.0. .. Wild-type and mutant Card1 proteins were diluted to the final concentrations of 11 to 41 μM in the isothermal titration calorimetry (ITC) buffer composed of 20 mM HEPES, pH 7.5, 150 mM NaCl, 0.5% β-mercaptoethanol, and were titrated against 150 to 500 μM cA4 or cA6 in the same buffer at 20 °C by using MicroCal PEAQ-ITC range (Malvern Panalytical and the High Throughput and Sequencing Center of Rockefeller University). ..

    High Throughput Screening Assay:

    Article Title: The Card1 nuclease provides defence during type III CRISPR immunity.
    Article Snippet: The UV absorbance data recorded at 260 nm were used, and final graphs are represented using GraphPad Prism v.8.0. .. Wild-type and mutant Card1 proteins were diluted to the final concentrations of 11 to 41 μM in the isothermal titration calorimetry (ITC) buffer composed of 20 mM HEPES, pH 7.5, 150 mM NaCl, 0.5% β-mercaptoethanol, and were titrated against 150 to 500 μM cA4 or cA6 in the same buffer at 20 °C by using MicroCal PEAQ-ITC range (Malvern Panalytical and the High Throughput and Sequencing Center of Rockefeller University). ..

    Sequencing:

    Article Title: The Card1 nuclease provides defence during type III CRISPR immunity.
    Article Snippet: The UV absorbance data recorded at 260 nm were used, and final graphs are represented using GraphPad Prism v.8.0. .. Wild-type and mutant Card1 proteins were diluted to the final concentrations of 11 to 41 μM in the isothermal titration calorimetry (ITC) buffer composed of 20 mM HEPES, pH 7.5, 150 mM NaCl, 0.5% β-mercaptoethanol, and were titrated against 150 to 500 μM cA4 or cA6 in the same buffer at 20 °C by using MicroCal PEAQ-ITC range (Malvern Panalytical and the High Throughput and Sequencing Center of Rockefeller University). ..



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    Image Search Results


    ( a ) Characterisation of BRD4 mutants that disrupt binding to CDK9-cyclin T1. Mutation of residues Gln1350, Leu1354 and Phe1357, highlighted by the FragLite map and AlphaFold3 model disrupt BRD4 binding to CDK9-Cyclin T1. ( b ) Mutation of BRD4 residues Leu1354 and Phe1357, leads to a loss of BRD4 binding to CDK9-cyclin T2 in a fluorescence polarisation (FP) assay. ( c ) The cyclin T1 Tyr175Ala mutation reduces the Homogenous Time-Resolved Fluorescence (HTRF) signal, whereas the Trp210Ala mutation, previously identified as important for AFF4 interaction and adjacent to Tyr175, shows signals comparable to wild-type. ( d-g ) Isothermal Titration Calorimetry (ITC) plots of CDK9-cyclin T2 complexes with BRD4. Representative titration plots for (d) CDK9-cyclin T2 (e) CDK9-cyclin T2 Tyr174Ala, (f) CDK9-cyclin T2 Phe175Ala (negative control), and (g) CDK9-cyclin T2 Trp206Ala vs the BRD4 P-TEFb Interaction Domain (PID). The top panels show the raw heat signal, and the bottom panels show the integrated heat per injection fitted to a single-site binding model. HTRF experiments were carried out in triplicate and repeated on three separate days. The error bars indicate SD. FP experiments were carried out in triplicate and repeated on three separate days. ITC thermodynamic parameters (K d ) were derived from three independent biological replicates. Derived K d values are compiled in . Related to and Supplementary Figure 11.

    Journal: bioRxiv

    Article Title: FragLite mapping to identify the BRD4 recruitment site of P-TEFb

    doi: 10.64898/2026.04.09.717428

    Figure Lengend Snippet: ( a ) Characterisation of BRD4 mutants that disrupt binding to CDK9-cyclin T1. Mutation of residues Gln1350, Leu1354 and Phe1357, highlighted by the FragLite map and AlphaFold3 model disrupt BRD4 binding to CDK9-Cyclin T1. ( b ) Mutation of BRD4 residues Leu1354 and Phe1357, leads to a loss of BRD4 binding to CDK9-cyclin T2 in a fluorescence polarisation (FP) assay. ( c ) The cyclin T1 Tyr175Ala mutation reduces the Homogenous Time-Resolved Fluorescence (HTRF) signal, whereas the Trp210Ala mutation, previously identified as important for AFF4 interaction and adjacent to Tyr175, shows signals comparable to wild-type. ( d-g ) Isothermal Titration Calorimetry (ITC) plots of CDK9-cyclin T2 complexes with BRD4. Representative titration plots for (d) CDK9-cyclin T2 (e) CDK9-cyclin T2 Tyr174Ala, (f) CDK9-cyclin T2 Phe175Ala (negative control), and (g) CDK9-cyclin T2 Trp206Ala vs the BRD4 P-TEFb Interaction Domain (PID). The top panels show the raw heat signal, and the bottom panels show the integrated heat per injection fitted to a single-site binding model. HTRF experiments were carried out in triplicate and repeated on three separate days. The error bars indicate SD. FP experiments were carried out in triplicate and repeated on three separate days. ITC thermodynamic parameters (K d ) were derived from three independent biological replicates. Derived K d values are compiled in . Related to and Supplementary Figure 11.

    Article Snippet: CDK9-cyclin T2 was buffer exchanged using a HiTrap desalting column (5 mL) (Cytiva) into ITC buffer (50 mM HEPES, 300 mM NaCl, 0.5 mM TCEP, pH 7.4) and protein concentration was then determined using a Nanodrop 2000 at an absorbance of 280 nm with sequence derived extinction coefficients ( http://web.expasy.org/ protparam/).

    Techniques: Binding Assay, Mutagenesis, Fluorescence, FP Assay, Isothermal Titration Calorimetry, Titration, Negative Control, Injection, Derivative Assay