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biotin-conjugated isolectin b 4 (ib 4  (Thermo Fisher)


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    Thermo Fisher biotin-conjugated isolectin b 4 (ib 4
    Double fluorescence of the GRP (green) and superficial layers markers (magenta) of Sp5C and cervical spinal dorsal horn in adult macaque monkeys. Many GRP-ir fibers were colocalized with the marker for peptidergic neurons CGRP (a–c) and substance P (d–f) in the Sp5C. GRP-ir fibers were not colocalized with the marker for nonpeptidergic neurons <t>IB</t> <t>4</t> in the Sp5C (g–i). Most GRP-ir fibers were colocalized with CGRP(j–l) and substance P (m–o) in the superficial layers of the cervical spinal cord. GRP-ir fibers were not colocalized with IB 4 (p–r) in the cervical spinal dorsal horn. Bars = 200 μm (i, r)
    Biotin Conjugated Isolectin B 4 (Ib 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolectin+b+4/pmc10228551-86-5-16?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    biotin-conjugated isolectin b 4 (ib 4 - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Characterization of the expression of gastrin-releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans"

    Article Title: Characterization of the expression of gastrin-releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans

    Journal: The Journal of comparative neurology

    doi: 10.1002/cne.25376

    Double fluorescence of the GRP (green) and superficial layers markers (magenta) of Sp5C and cervical spinal dorsal horn in adult macaque monkeys. Many GRP-ir fibers were colocalized with the marker for peptidergic neurons CGRP (a–c) and substance P (d–f) in the Sp5C. GRP-ir fibers were not colocalized with the marker for nonpeptidergic neurons IB 4 in the Sp5C (g–i). Most GRP-ir fibers were colocalized with CGRP(j–l) and substance P (m–o) in the superficial layers of the cervical spinal cord. GRP-ir fibers were not colocalized with IB 4 (p–r) in the cervical spinal dorsal horn. Bars = 200 μm (i, r)
    Figure Legend Snippet: Double fluorescence of the GRP (green) and superficial layers markers (magenta) of Sp5C and cervical spinal dorsal horn in adult macaque monkeys. Many GRP-ir fibers were colocalized with the marker for peptidergic neurons CGRP (a–c) and substance P (d–f) in the Sp5C. GRP-ir fibers were not colocalized with the marker for nonpeptidergic neurons IB 4 in the Sp5C (g–i). Most GRP-ir fibers were colocalized with CGRP(j–l) and substance P (m–o) in the superficial layers of the cervical spinal cord. GRP-ir fibers were not colocalized with IB 4 (p–r) in the cervical spinal dorsal horn. Bars = 200 μm (i, r)

    Techniques Used: Fluorescence, Marker



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    biotin-conjugated isolectin b 4 (ib 4  (Thermo Fisher)
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    Thermo Fisher biotin-conjugated isolectin b 4 (ib 4
    Double fluorescence of the GRP (green) and superficial layers markers (magenta) of Sp5C and cervical spinal dorsal horn in adult macaque monkeys. Many GRP-ir fibers were colocalized with the marker for peptidergic neurons CGRP (a–c) and substance P (d–f) in the Sp5C. GRP-ir fibers were not colocalized with the marker for nonpeptidergic neurons <t>IB</t> <t>4</t> in the Sp5C (g–i). Most GRP-ir fibers were colocalized with CGRP(j–l) and substance P (m–o) in the superficial layers of the cervical spinal cord. GRP-ir fibers were not colocalized with IB 4 (p–r) in the cervical spinal dorsal horn. Bars = 200 μm (i, r)
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    Image Search Results


    Timp2 , Timp3 , and Timp4 are highly expressed in slowly dividing NPCs. (A) The scheme for isolating rapidly dividing and slowly dividing NPCs. H2B-GFP was transiently induced at E9.5 and the LGE was dissected at E17.5. NPCs were defined as cells positive for NPC marker CD133, negative for neuronal marker CD24, and negative for endothelial marker Isolectin B 4 . (B) Representative flow cytometric histogram of H2B-GFP fluorescence intensity. Rapidly dividing NPCs (bottom 10% of NPCs for H2B-GFP intensity) and slowly dividing NPCs (top 10% of NPCs for H2B-GFP intensity) were collected. (C) Quantitative RT-PCR analysis of Timp2 , Timp3 , and Timp4 mRNAs. Data are means ± SEM ( n = 3 independent experiments). * p < 0.05 by two-tailed paired t test.

    Journal: Frontiers in Neuroscience

    Article Title: TIMP3 promotes the maintenance of neural stem-progenitor cells in the mouse subventricular zone

    doi: 10.3389/fnins.2023.1149603

    Figure Lengend Snippet: Timp2 , Timp3 , and Timp4 are highly expressed in slowly dividing NPCs. (A) The scheme for isolating rapidly dividing and slowly dividing NPCs. H2B-GFP was transiently induced at E9.5 and the LGE was dissected at E17.5. NPCs were defined as cells positive for NPC marker CD133, negative for neuronal marker CD24, and negative for endothelial marker Isolectin B 4 . (B) Representative flow cytometric histogram of H2B-GFP fluorescence intensity. Rapidly dividing NPCs (bottom 10% of NPCs for H2B-GFP intensity) and slowly dividing NPCs (top 10% of NPCs for H2B-GFP intensity) were collected. (C) Quantitative RT-PCR analysis of Timp2 , Timp3 , and Timp4 mRNAs. Data are means ± SEM ( n = 3 independent experiments). * p < 0.05 by two-tailed paired t test.

    Article Snippet: Dissociated cells were incubated first for 15 min on ice in 0.2% bovine serum albumin (BSA)/phosphate-buffered saline (PBS) with primary antibodies—PE/Cy7 anti-CD133 (1:100, BioLegend Cat# 141210, RRID:AB_2564069), APC anti-CD24 (1,100, BioLegend Cat# 101814, RRID:AB_439716), and anti-Isolectin B 4 (biotin conjugate) (1,1,000, Sigma-Aldrich Cat# L2140, RRID:AB_2313663)—and then for 5 min on ice in 0.2% BSA/PBS with Streptavidin PE (1,500, eBioscience Cat# 12-4317-87).

    Techniques: Marker, Fluorescence, Quantitative RT-PCR, Two Tailed Test

    Double fluorescence of the GRP (green) and superficial layers markers (magenta) of Sp5C and cervical spinal dorsal horn in adult macaque monkeys. Many GRP-ir fibers were colocalized with the marker for peptidergic neurons CGRP (a–c) and substance P (d–f) in the Sp5C. GRP-ir fibers were not colocalized with the marker for nonpeptidergic neurons IB 4 in the Sp5C (g–i). Most GRP-ir fibers were colocalized with CGRP(j–l) and substance P (m–o) in the superficial layers of the cervical spinal cord. GRP-ir fibers were not colocalized with IB 4 (p–r) in the cervical spinal dorsal horn. Bars = 200 μm (i, r)

    Journal: The Journal of comparative neurology

    Article Title: Characterization of the expression of gastrin-releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans

    doi: 10.1002/cne.25376

    Figure Lengend Snippet: Double fluorescence of the GRP (green) and superficial layers markers (magenta) of Sp5C and cervical spinal dorsal horn in adult macaque monkeys. Many GRP-ir fibers were colocalized with the marker for peptidergic neurons CGRP (a–c) and substance P (d–f) in the Sp5C. GRP-ir fibers were not colocalized with the marker for nonpeptidergic neurons IB 4 in the Sp5C (g–i). Most GRP-ir fibers were colocalized with CGRP(j–l) and substance P (m–o) in the superficial layers of the cervical spinal cord. GRP-ir fibers were not colocalized with IB 4 (p–r) in the cervical spinal dorsal horn. Bars = 200 μm (i, r)

    Article Snippet: Double-fluorescence staining of GRP and biotin-conjugated isolectin B 4 (IB 4 ) (1:1,000; cat. no. 121414; Molecular Probes, Eugene, OR) was also performed.

    Techniques: Fluorescence, Marker