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magnetic separation kit  (Miltenyi Biotec)


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    Miltenyi Biotec magnetic separation kit
    Magnetic Separation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 779 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolators/Pan+Monocyte+Isolation+Kit%2C+human/pmc12924898-249-4-7
    Average 96 stars, based on 779 article reviews
    magnetic separation kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Itaconate negatively regulates innate immunity during fungal pneumonia.
    Article Snippet: Peripheral blood samples (10 mL) from healthy human volunteers were drawn into BD Vacutainer Blood Collection Tubes (EDTA K2) and peripheral blood mononuclear cells isolated via Ficoll-Paque PLUS (MilliporeSigma, Catalog #GE17-1440-02). .. Monocytes were isolated using a human Pan Monocyte Isolation Kit (Miltenyi Biotec Catalog #130-096-537) and cultured with human recombinant M-CSF (50 ng/mL; STEMCELL Technologies Catalog #78057) and ImmunoCult-SF Macrophage Medium (STEMCELL Technologies, Catalog #10961) for 4 days. ..

    Article Title: A 3D Human Bone and Bone Marrow-on-a-Chip Model for In Vitro Bone Remodeling and Immune Cell Maintenance.
    Article Snippet: .. After isolation of BMMNCs or peripheral blood mononuclear cells (PBMCs) from primary BM or blood, enrichment of classical (CD14++ CD16− ), nonclassical (CD14+ CD16++ ), and intermediate (CD14++ CD16+ ) monocytes was performed using the Pan monocyte isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: NET-derived DNA is associated with cGAS-STING and type I IFN responses, as supported by monocyte stimulation cultures using plasma from female patients with SLE.
    Article Snippet: Monocytes were subsequently enriched using an autoMACS Separator (Miltenyi Biotec, Cat. No. 130092545) according to the manufacturer’s protocol. .. Negative selection was performed using the Pan Monocyte Isolation Kit (Miltenyi Biotec, Cat. No. 130096537). .. Cells were cultured in RPMI 1640 medium (Gibco, Cat. No. 52400025) supplemented with 10% (v/v) fetal bovine serum (FBS; Capricorn Scientific, Lot.

    Article Title: Neonatal monocyte iron metabolism and immunometabolic responses in sepsis: a comparative ex vivo investigation.
    Article Snippet: .. CD14+ monocytes were enriched via negative-selection magneticactivated cell sorting using the Pan Monocyte Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. .. CD14+ monocytes were enriched via negative-selection magneticactivated cell sorting using the Pan Monocyte Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions.

    Article Title: Cytotoxic lymphocyte death drives monocyte activation and TNF/IFN-γ synergism in TBK1 deficiency
    Article Snippet: Cell Death and Disease Cell isolation, culture, and stimulation Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood of the patient and healthy controls by density gradient centrifugation using LSM lymphocyte separation medium (MP Bio, 50494X) according to the manufacturer’s instructions. .. Primary T lymphocytes and monocytes were purified from PBMCs by negative selection using the Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535) and Pan Monocyte Isolation Kit (Miltenyi Biotec, 130-096-537), respectively, according to the manufacturer’s instructions. .. Primary monocytes were differentiated with GM-CSF (20 ng/mL, R&D, 7954-GM-010) over 10 days to generate human monocyte-derived macrophages (hMDMs).

    Article Title: Dopamine-induced mitochondrial dysfunction activates cGAS-sTING to drive IL-6 secretion in macrophages.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-DRP1 (Ser616) Rabbit Polyclonal Antibody Cell Signaling Technology Cat#3455; RRID: AB_2085352 DRP1 (D6C7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#8570; RRID: AB_10950498 cGAS (D1D3G) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#15102; RRID: AB_2732795 Phospho-STING (Ser366) (E9A9K) Cell Signaling Technology Cat#50907; RRID: AB_2827656 STING (D2P2F) Cell Signaling Technology Cat#13647; RRID: AB_2827656 phospho-TBK1 (Ser172) Polyclonal Antibody Thermo Fisher Scientific Cat #PA5-105919; RRID: AB_2817318 TBK1 Recombinant Rabbit Monoclonal Antibody (12H60L39) Thermo Fisher Scientific Cat #703154; RRID: AB_2848223 Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody (6D3D1) Thermo Fisher Scientific Cat #MA5-35850; RRID: AB_2849749 IRF3 Recombinant Rabbit Monoclonal Antibody (3H32L10) Thermo Fisher Scientific Cat #703682; RRID: AB_2784599 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling Technology Cat #4060; RRID: AB_2315049 Akt Antibody Cell Signaling Technology Cat #9272; RRID: AB_329827 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb Cell Signaling Technology Cat #4511; RRID: AB_2139682 Anti-p38 MAPK Antibody Cell Signaling Technology Cat #9212; RRID: AB_330713 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat #4370; RRID: AB_2315112 Mouse Anti-p44/42 MAP Kinase Monoclonal Antibody, Unconjugated, Clone L34F12 Cell Signaling Technology Cat #4696; RRID: AB_390780 Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb Cell Signaling Technology Cat #9323; RRID: AB_2115201 GSK-3beta (27C10) Rabbit mAb Cell Signaling Technology Cat #9315; RRID: AB_490890 Parkin (Prk8) Mouse monoclonal Antibody Cell Signaling Technology Cat #4211; RRID: AB_2159920 PINK1 (D8G3) Rabbit mAb Cell Signaling Technology Cat #6946; RRID: AB_11179069 Anti-BNIP3L/Nix (D4R4B) Rabbit Antibody Cell Signaling Technology Cat #12396; RRID: AB_2688036 LC3B (D11) XP Rabbit mAb Cell Signaling Technology Cat #3868; RRID: AB_2137707 NF-κB p65 (D14E12) XP Rabbit mAb Cell Signaling Technology Cat #8242; RRID: AB_10859369 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat #A-11008; RRID: AB_143165 LAMP1 (D2D11) XP Rabbit Antibody Cell Signaling Technology Cat #9091; RRID: AB_2687579 Recombinant Anti-TOMM20 antibody [EPR15581-39] Abcam Cat #ab289670; RRID: AB_2943038 Beta-Actin (13E5) Rabbit Monoclonal Antibody Cell Signaling Technology Cat #4970; RRID: AB_2223172 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat #11967; RRID: AB_2797784 Biological samples Fresh human blood New York Blood Center N/A Chemicals, peptides, and recombinant proteins Macrophage colony stimulating factor PeproTech Cat #315–02 Dopamine hydrochloride Sigma-Aldrich Cat #H8502 Lipopolysaccharide from Escherichia coli O55:B5 MilliporeSigma Cat #L2880 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Tocris Cat #0453 2′3′-cGAMP MedChemExpress Cat #HY-12512 Mdivi-1 MedChemExpress Cat #HY-15886 G-140 MedChemExpress Cat #HY-133916 H-151 MedChemExpress Cat #HY-112693 Ethidium bromide MedChemExpress Cat #HY-D0021 (Continued on next page) 20 Cell Reports 45, 118118, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BAY 11–7082 Selleck Chemicals Cat #S2913 JC-1 Thermo Fisher Scientific Cat #T3168 MitoSOXTM Red mitochondrial superoxide indicator Thermo Fisher Scientific Cat #M36008 Zombie NIRTM fixable viability dye BioLegend Cat #423105 SKF38393 Hydrochloride EMD Millipore Cat #505881 Quinpirole hydrochloride Tocris Cat #1061/10 Apomorphine hydrochloride Tocris Cat #2073/50 SCH23390 hydrochloride Tocris Cat #0925 Eticlopride hydrochloride Tocris Cat #1847 Accell green Non-targeting siRNA Horizon Discovery Cat #D-001950–01-05 Accell Human TMEM173 (340061) siRNA - SMARTpool Horizon Discovery Cat #E− 024333-00-0010 Critical commercial assays Pan Monocyte Isolation Kit Miltenyi Biotec Cat #130–096-537 AlphaLISA Human IL-6 Detection Kit Revvity Cat #AL223C MSD V-Plex Human Neuroinflammation Panel Meso Scale Diagnostics Cat #K15210D-1 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat #A55864 Human JAK/STAT Pathway Phosphorylation Array C1 RayBiotech Cat #AAH-JAKSTAT-1-8 XF Real-Time ATP Rate Assay Kit Agilent Cat #103592–100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat #103015–100 Mitochondria/Cytosol Fractionation Kit Abcam Cat #ab65320 Quick-DNA/RNATM Miniprep Kit Zymo Research Cat #D7001 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat #4368814 AlphaLISA SureFire Ultra Human Total STING Detection Kit Revvity Cat # ALSU-TSTNG-A10K Deposited data Mass spectrometry data ProteomeXchange Consortium; MassIVE repository PXD077267 Code for the principal component analysis and cluster analysis Zenodo https://doi.org/10.5281/ zenodo.19670069 Oligonucleotides DRD1 Thermo Fisher Scientific Cat #Hs00265245_s1 DRD2 Thermo Fisher Scientific Cat #Hs00241436_m1 DRD3 Thermo Fisher Scientific Cat #Hs00364455_m1 DRD4 Thermo Fisher Scientific Cat #Hs00609526_m1 DRD5 Thermo Fisher Scientific Cat #Hs00361234_s1 IFIT1 Thermo Fisher Scientific Cat #Hs01675197_m1 ISG15 Thermo Fisher Scientific Cat #Hs01921425_s1 18S Thermo Fisher Scientific Cat #4319413E Cytochrome c oxidase IDT DNA F: 5′GCCCCAGATATAGCATT CCC-3′ R: 5′-GTTCATCCTGTTCCTGC TCC-3′ Nuclear 18S IDT DNA F: 5′-TAGAGGGACAAGTGG CGTTC-3′ R: 5′CGCTGAGCCAG TCAGTGT-3′ Software and algorithms MSD Discovery Workbench Software version 4.0 Meso Scale Diagnostics N/A Image StudioTM Lite LI-COR Biosciences N/A DIA-NN (v2.2.0) N/A N/A ..

    Cell Culture:

    Article Title: Itaconate negatively regulates innate immunity during fungal pneumonia.
    Article Snippet: Peripheral blood samples (10 mL) from healthy human volunteers were drawn into BD Vacutainer Blood Collection Tubes (EDTA K2) and peripheral blood mononuclear cells isolated via Ficoll-Paque PLUS (MilliporeSigma, Catalog #GE17-1440-02). .. Monocytes were isolated using a human Pan Monocyte Isolation Kit (Miltenyi Biotec Catalog #130-096-537) and cultured with human recombinant M-CSF (50 ng/mL; STEMCELL Technologies Catalog #78057) and ImmunoCult-SF Macrophage Medium (STEMCELL Technologies, Catalog #10961) for 4 days. ..

    Recombinant:

    Article Title: Itaconate negatively regulates innate immunity during fungal pneumonia.
    Article Snippet: Peripheral blood samples (10 mL) from healthy human volunteers were drawn into BD Vacutainer Blood Collection Tubes (EDTA K2) and peripheral blood mononuclear cells isolated via Ficoll-Paque PLUS (MilliporeSigma, Catalog #GE17-1440-02). .. Monocytes were isolated using a human Pan Monocyte Isolation Kit (Miltenyi Biotec Catalog #130-096-537) and cultured with human recombinant M-CSF (50 ng/mL; STEMCELL Technologies Catalog #78057) and ImmunoCult-SF Macrophage Medium (STEMCELL Technologies, Catalog #10961) for 4 days. ..

    Selection:

    Article Title: NET-derived DNA is associated with cGAS-STING and type I IFN responses, as supported by monocyte stimulation cultures using plasma from female patients with SLE.
    Article Snippet: Monocytes were subsequently enriched using an autoMACS Separator (Miltenyi Biotec, Cat. No. 130092545) according to the manufacturer’s protocol. .. Negative selection was performed using the Pan Monocyte Isolation Kit (Miltenyi Biotec, Cat. No. 130096537). .. Cells were cultured in RPMI 1640 medium (Gibco, Cat. No. 52400025) supplemented with 10% (v/v) fetal bovine serum (FBS; Capricorn Scientific, Lot.

    Article Title: CSF1R regulates monocyte subset differentiation and intracellular metabolism.
    Article Snippet: .. Human cells Monocyte enrichment from human peripheral blood was performed using the autoMACS® Pro Separator (Miltenyi, France) and a negative selection method with an antibody cocktail targeting all mature cells except monocytes (Miltenyi, 130-096-537). .. Purified monocytes were grown in RPMI 1640 medium with glutamax-I (Life Technologies, 61870044) supplemented with 10% (vol/vol) fetal bovine serum (Life Technologies) and treated with Gilteritinib (1μM), PLX5622 (5nM), or a combination of both for 16h.

    Article Title: Cytotoxic lymphocyte death drives monocyte activation and TNF/IFN-γ synergism in TBK1 deficiency
    Article Snippet: Cell Death and Disease Cell isolation, culture, and stimulation Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood of the patient and healthy controls by density gradient centrifugation using LSM lymphocyte separation medium (MP Bio, 50494X) according to the manufacturer’s instructions. .. Primary T lymphocytes and monocytes were purified from PBMCs by negative selection using the Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535) and Pan Monocyte Isolation Kit (Miltenyi Biotec, 130-096-537), respectively, according to the manufacturer’s instructions. .. Primary monocytes were differentiated with GM-CSF (20 ng/mL, R&D, 7954-GM-010) over 10 days to generate human monocyte-derived macrophages (hMDMs).

    FACS:

    Article Title: Neonatal monocyte iron metabolism and immunometabolic responses in sepsis: a comparative ex vivo investigation.
    Article Snippet: .. CD14+ monocytes were enriched via negative-selection magneticactivated cell sorting using the Pan Monocyte Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. .. CD14+ monocytes were enriched via negative-selection magneticactivated cell sorting using the Pan Monocyte Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions.

    Purification:

    Article Title: Cytotoxic lymphocyte death drives monocyte activation and TNF/IFN-γ synergism in TBK1 deficiency
    Article Snippet: Cell Death and Disease Cell isolation, culture, and stimulation Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood of the patient and healthy controls by density gradient centrifugation using LSM lymphocyte separation medium (MP Bio, 50494X) according to the manufacturer’s instructions. .. Primary T lymphocytes and monocytes were purified from PBMCs by negative selection using the Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535) and Pan Monocyte Isolation Kit (Miltenyi Biotec, 130-096-537), respectively, according to the manufacturer’s instructions. .. Primary monocytes were differentiated with GM-CSF (20 ng/mL, R&D, 7954-GM-010) over 10 days to generate human monocyte-derived macrophages (hMDMs).

    Cell Isolation:

    Article Title: Cytotoxic lymphocyte death drives monocyte activation and TNF/IFN-γ synergism in TBK1 deficiency
    Article Snippet: Cell Death and Disease Cell isolation, culture, and stimulation Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood of the patient and healthy controls by density gradient centrifugation using LSM lymphocyte separation medium (MP Bio, 50494X) according to the manufacturer’s instructions. .. Primary T lymphocytes and monocytes were purified from PBMCs by negative selection using the Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535) and Pan Monocyte Isolation Kit (Miltenyi Biotec, 130-096-537), respectively, according to the manufacturer’s instructions. .. Primary monocytes were differentiated with GM-CSF (20 ng/mL, R&D, 7954-GM-010) over 10 days to generate human monocyte-derived macrophages (hMDMs).

    BIA-KA:

    Article Title: Dopamine-induced mitochondrial dysfunction activates cGAS-sTING to drive IL-6 secretion in macrophages.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-DRP1 (Ser616) Rabbit Polyclonal Antibody Cell Signaling Technology Cat#3455; RRID: AB_2085352 DRP1 (D6C7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#8570; RRID: AB_10950498 cGAS (D1D3G) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#15102; RRID: AB_2732795 Phospho-STING (Ser366) (E9A9K) Cell Signaling Technology Cat#50907; RRID: AB_2827656 STING (D2P2F) Cell Signaling Technology Cat#13647; RRID: AB_2827656 phospho-TBK1 (Ser172) Polyclonal Antibody Thermo Fisher Scientific Cat #PA5-105919; RRID: AB_2817318 TBK1 Recombinant Rabbit Monoclonal Antibody (12H60L39) Thermo Fisher Scientific Cat #703154; RRID: AB_2848223 Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody (6D3D1) Thermo Fisher Scientific Cat #MA5-35850; RRID: AB_2849749 IRF3 Recombinant Rabbit Monoclonal Antibody (3H32L10) Thermo Fisher Scientific Cat #703682; RRID: AB_2784599 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling Technology Cat #4060; RRID: AB_2315049 Akt Antibody Cell Signaling Technology Cat #9272; RRID: AB_329827 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb Cell Signaling Technology Cat #4511; RRID: AB_2139682 Anti-p38 MAPK Antibody Cell Signaling Technology Cat #9212; RRID: AB_330713 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat #4370; RRID: AB_2315112 Mouse Anti-p44/42 MAP Kinase Monoclonal Antibody, Unconjugated, Clone L34F12 Cell Signaling Technology Cat #4696; RRID: AB_390780 Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb Cell Signaling Technology Cat #9323; RRID: AB_2115201 GSK-3beta (27C10) Rabbit mAb Cell Signaling Technology Cat #9315; RRID: AB_490890 Parkin (Prk8) Mouse monoclonal Antibody Cell Signaling Technology Cat #4211; RRID: AB_2159920 PINK1 (D8G3) Rabbit mAb Cell Signaling Technology Cat #6946; RRID: AB_11179069 Anti-BNIP3L/Nix (D4R4B) Rabbit Antibody Cell Signaling Technology Cat #12396; RRID: AB_2688036 LC3B (D11) XP Rabbit mAb Cell Signaling Technology Cat #3868; RRID: AB_2137707 NF-κB p65 (D14E12) XP Rabbit mAb Cell Signaling Technology Cat #8242; RRID: AB_10859369 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat #A-11008; RRID: AB_143165 LAMP1 (D2D11) XP Rabbit Antibody Cell Signaling Technology Cat #9091; RRID: AB_2687579 Recombinant Anti-TOMM20 antibody [EPR15581-39] Abcam Cat #ab289670; RRID: AB_2943038 Beta-Actin (13E5) Rabbit Monoclonal Antibody Cell Signaling Technology Cat #4970; RRID: AB_2223172 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat #11967; RRID: AB_2797784 Biological samples Fresh human blood New York Blood Center N/A Chemicals, peptides, and recombinant proteins Macrophage colony stimulating factor PeproTech Cat #315–02 Dopamine hydrochloride Sigma-Aldrich Cat #H8502 Lipopolysaccharide from Escherichia coli O55:B5 MilliporeSigma Cat #L2880 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Tocris Cat #0453 2′3′-cGAMP MedChemExpress Cat #HY-12512 Mdivi-1 MedChemExpress Cat #HY-15886 G-140 MedChemExpress Cat #HY-133916 H-151 MedChemExpress Cat #HY-112693 Ethidium bromide MedChemExpress Cat #HY-D0021 (Continued on next page) 20 Cell Reports 45, 118118, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BAY 11–7082 Selleck Chemicals Cat #S2913 JC-1 Thermo Fisher Scientific Cat #T3168 MitoSOXTM Red mitochondrial superoxide indicator Thermo Fisher Scientific Cat #M36008 Zombie NIRTM fixable viability dye BioLegend Cat #423105 SKF38393 Hydrochloride EMD Millipore Cat #505881 Quinpirole hydrochloride Tocris Cat #1061/10 Apomorphine hydrochloride Tocris Cat #2073/50 SCH23390 hydrochloride Tocris Cat #0925 Eticlopride hydrochloride Tocris Cat #1847 Accell green Non-targeting siRNA Horizon Discovery Cat #D-001950–01-05 Accell Human TMEM173 (340061) siRNA - SMARTpool Horizon Discovery Cat #E− 024333-00-0010 Critical commercial assays Pan Monocyte Isolation Kit Miltenyi Biotec Cat #130–096-537 AlphaLISA Human IL-6 Detection Kit Revvity Cat #AL223C MSD V-Plex Human Neuroinflammation Panel Meso Scale Diagnostics Cat #K15210D-1 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat #A55864 Human JAK/STAT Pathway Phosphorylation Array C1 RayBiotech Cat #AAH-JAKSTAT-1-8 XF Real-Time ATP Rate Assay Kit Agilent Cat #103592–100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat #103015–100 Mitochondria/Cytosol Fractionation Kit Abcam Cat #ab65320 Quick-DNA/RNATM Miniprep Kit Zymo Research Cat #D7001 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat #4368814 AlphaLISA SureFire Ultra Human Total STING Detection Kit Revvity Cat # ALSU-TSTNG-A10K Deposited data Mass spectrometry data ProteomeXchange Consortium; MassIVE repository PXD077267 Code for the principal component analysis and cluster analysis Zenodo https://doi.org/10.5281/ zenodo.19670069 Oligonucleotides DRD1 Thermo Fisher Scientific Cat #Hs00265245_s1 DRD2 Thermo Fisher Scientific Cat #Hs00241436_m1 DRD3 Thermo Fisher Scientific Cat #Hs00364455_m1 DRD4 Thermo Fisher Scientific Cat #Hs00609526_m1 DRD5 Thermo Fisher Scientific Cat #Hs00361234_s1 IFIT1 Thermo Fisher Scientific Cat #Hs01675197_m1 ISG15 Thermo Fisher Scientific Cat #Hs01921425_s1 18S Thermo Fisher Scientific Cat #4319413E Cytochrome c oxidase IDT DNA F: 5′GCCCCAGATATAGCATT CCC-3′ R: 5′-GTTCATCCTGTTCCTGC TCC-3′ Nuclear 18S IDT DNA F: 5′-TAGAGGGACAAGTGG CGTTC-3′ R: 5′CGCTGAGCCAG TCAGTGT-3′ Software and algorithms MSD Discovery Workbench Software version 4.0 Meso Scale Diagnostics N/A Image StudioTM Lite LI-COR Biosciences N/A DIA-NN (v2.2.0) N/A N/A ..

    Phospho-proteomics:

    Article Title: Dopamine-induced mitochondrial dysfunction activates cGAS-sTING to drive IL-6 secretion in macrophages.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-DRP1 (Ser616) Rabbit Polyclonal Antibody Cell Signaling Technology Cat#3455; RRID: AB_2085352 DRP1 (D6C7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#8570; RRID: AB_10950498 cGAS (D1D3G) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#15102; RRID: AB_2732795 Phospho-STING (Ser366) (E9A9K) Cell Signaling Technology Cat#50907; RRID: AB_2827656 STING (D2P2F) Cell Signaling Technology Cat#13647; RRID: AB_2827656 phospho-TBK1 (Ser172) Polyclonal Antibody Thermo Fisher Scientific Cat #PA5-105919; RRID: AB_2817318 TBK1 Recombinant Rabbit Monoclonal Antibody (12H60L39) Thermo Fisher Scientific Cat #703154; RRID: AB_2848223 Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody (6D3D1) Thermo Fisher Scientific Cat #MA5-35850; RRID: AB_2849749 IRF3 Recombinant Rabbit Monoclonal Antibody (3H32L10) Thermo Fisher Scientific Cat #703682; RRID: AB_2784599 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling Technology Cat #4060; RRID: AB_2315049 Akt Antibody Cell Signaling Technology Cat #9272; RRID: AB_329827 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb Cell Signaling Technology Cat #4511; RRID: AB_2139682 Anti-p38 MAPK Antibody Cell Signaling Technology Cat #9212; RRID: AB_330713 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat #4370; RRID: AB_2315112 Mouse Anti-p44/42 MAP Kinase Monoclonal Antibody, Unconjugated, Clone L34F12 Cell Signaling Technology Cat #4696; RRID: AB_390780 Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb Cell Signaling Technology Cat #9323; RRID: AB_2115201 GSK-3beta (27C10) Rabbit mAb Cell Signaling Technology Cat #9315; RRID: AB_490890 Parkin (Prk8) Mouse monoclonal Antibody Cell Signaling Technology Cat #4211; RRID: AB_2159920 PINK1 (D8G3) Rabbit mAb Cell Signaling Technology Cat #6946; RRID: AB_11179069 Anti-BNIP3L/Nix (D4R4B) Rabbit Antibody Cell Signaling Technology Cat #12396; RRID: AB_2688036 LC3B (D11) XP Rabbit mAb Cell Signaling Technology Cat #3868; RRID: AB_2137707 NF-κB p65 (D14E12) XP Rabbit mAb Cell Signaling Technology Cat #8242; RRID: AB_10859369 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat #A-11008; RRID: AB_143165 LAMP1 (D2D11) XP Rabbit Antibody Cell Signaling Technology Cat #9091; RRID: AB_2687579 Recombinant Anti-TOMM20 antibody [EPR15581-39] Abcam Cat #ab289670; RRID: AB_2943038 Beta-Actin (13E5) Rabbit Monoclonal Antibody Cell Signaling Technology Cat #4970; RRID: AB_2223172 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat #11967; RRID: AB_2797784 Biological samples Fresh human blood New York Blood Center N/A Chemicals, peptides, and recombinant proteins Macrophage colony stimulating factor PeproTech Cat #315–02 Dopamine hydrochloride Sigma-Aldrich Cat #H8502 Lipopolysaccharide from Escherichia coli O55:B5 MilliporeSigma Cat #L2880 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Tocris Cat #0453 2′3′-cGAMP MedChemExpress Cat #HY-12512 Mdivi-1 MedChemExpress Cat #HY-15886 G-140 MedChemExpress Cat #HY-133916 H-151 MedChemExpress Cat #HY-112693 Ethidium bromide MedChemExpress Cat #HY-D0021 (Continued on next page) 20 Cell Reports 45, 118118, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BAY 11–7082 Selleck Chemicals Cat #S2913 JC-1 Thermo Fisher Scientific Cat #T3168 MitoSOXTM Red mitochondrial superoxide indicator Thermo Fisher Scientific Cat #M36008 Zombie NIRTM fixable viability dye BioLegend Cat #423105 SKF38393 Hydrochloride EMD Millipore Cat #505881 Quinpirole hydrochloride Tocris Cat #1061/10 Apomorphine hydrochloride Tocris Cat #2073/50 SCH23390 hydrochloride Tocris Cat #0925 Eticlopride hydrochloride Tocris Cat #1847 Accell green Non-targeting siRNA Horizon Discovery Cat #D-001950–01-05 Accell Human TMEM173 (340061) siRNA - SMARTpool Horizon Discovery Cat #E− 024333-00-0010 Critical commercial assays Pan Monocyte Isolation Kit Miltenyi Biotec Cat #130–096-537 AlphaLISA Human IL-6 Detection Kit Revvity Cat #AL223C MSD V-Plex Human Neuroinflammation Panel Meso Scale Diagnostics Cat #K15210D-1 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat #A55864 Human JAK/STAT Pathway Phosphorylation Array C1 RayBiotech Cat #AAH-JAKSTAT-1-8 XF Real-Time ATP Rate Assay Kit Agilent Cat #103592–100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat #103015–100 Mitochondria/Cytosol Fractionation Kit Abcam Cat #ab65320 Quick-DNA/RNATM Miniprep Kit Zymo Research Cat #D7001 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat #4368814 AlphaLISA SureFire Ultra Human Total STING Detection Kit Revvity Cat # ALSU-TSTNG-A10K Deposited data Mass spectrometry data ProteomeXchange Consortium; MassIVE repository PXD077267 Code for the principal component analysis and cluster analysis Zenodo https://doi.org/10.5281/ zenodo.19670069 Oligonucleotides DRD1 Thermo Fisher Scientific Cat #Hs00265245_s1 DRD2 Thermo Fisher Scientific Cat #Hs00241436_m1 DRD3 Thermo Fisher Scientific Cat #Hs00364455_m1 DRD4 Thermo Fisher Scientific Cat #Hs00609526_m1 DRD5 Thermo Fisher Scientific Cat #Hs00361234_s1 IFIT1 Thermo Fisher Scientific Cat #Hs01675197_m1 ISG15 Thermo Fisher Scientific Cat #Hs01921425_s1 18S Thermo Fisher Scientific Cat #4319413E Cytochrome c oxidase IDT DNA F: 5′GCCCCAGATATAGCATT CCC-3′ R: 5′-GTTCATCCTGTTCCTGC TCC-3′ Nuclear 18S IDT DNA F: 5′-TAGAGGGACAAGTGG CGTTC-3′ R: 5′CGCTGAGCCAG TCAGTGT-3′ Software and algorithms MSD Discovery Workbench Software version 4.0 Meso Scale Diagnostics N/A Image StudioTM Lite LI-COR Biosciences N/A DIA-NN (v2.2.0) N/A N/A ..

    Fractionation:

    Article Title: Dopamine-induced mitochondrial dysfunction activates cGAS-sTING to drive IL-6 secretion in macrophages.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-DRP1 (Ser616) Rabbit Polyclonal Antibody Cell Signaling Technology Cat#3455; RRID: AB_2085352 DRP1 (D6C7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#8570; RRID: AB_10950498 cGAS (D1D3G) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#15102; RRID: AB_2732795 Phospho-STING (Ser366) (E9A9K) Cell Signaling Technology Cat#50907; RRID: AB_2827656 STING (D2P2F) Cell Signaling Technology Cat#13647; RRID: AB_2827656 phospho-TBK1 (Ser172) Polyclonal Antibody Thermo Fisher Scientific Cat #PA5-105919; RRID: AB_2817318 TBK1 Recombinant Rabbit Monoclonal Antibody (12H60L39) Thermo Fisher Scientific Cat #703154; RRID: AB_2848223 Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody (6D3D1) Thermo Fisher Scientific Cat #MA5-35850; RRID: AB_2849749 IRF3 Recombinant Rabbit Monoclonal Antibody (3H32L10) Thermo Fisher Scientific Cat #703682; RRID: AB_2784599 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling Technology Cat #4060; RRID: AB_2315049 Akt Antibody Cell Signaling Technology Cat #9272; RRID: AB_329827 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb Cell Signaling Technology Cat #4511; RRID: AB_2139682 Anti-p38 MAPK Antibody Cell Signaling Technology Cat #9212; RRID: AB_330713 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat #4370; RRID: AB_2315112 Mouse Anti-p44/42 MAP Kinase Monoclonal Antibody, Unconjugated, Clone L34F12 Cell Signaling Technology Cat #4696; RRID: AB_390780 Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb Cell Signaling Technology Cat #9323; RRID: AB_2115201 GSK-3beta (27C10) Rabbit mAb Cell Signaling Technology Cat #9315; RRID: AB_490890 Parkin (Prk8) Mouse monoclonal Antibody Cell Signaling Technology Cat #4211; RRID: AB_2159920 PINK1 (D8G3) Rabbit mAb Cell Signaling Technology Cat #6946; RRID: AB_11179069 Anti-BNIP3L/Nix (D4R4B) Rabbit Antibody Cell Signaling Technology Cat #12396; RRID: AB_2688036 LC3B (D11) XP Rabbit mAb Cell Signaling Technology Cat #3868; RRID: AB_2137707 NF-κB p65 (D14E12) XP Rabbit mAb Cell Signaling Technology Cat #8242; RRID: AB_10859369 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat #A-11008; RRID: AB_143165 LAMP1 (D2D11) XP Rabbit Antibody Cell Signaling Technology Cat #9091; RRID: AB_2687579 Recombinant Anti-TOMM20 antibody [EPR15581-39] Abcam Cat #ab289670; RRID: AB_2943038 Beta-Actin (13E5) Rabbit Monoclonal Antibody Cell Signaling Technology Cat #4970; RRID: AB_2223172 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat #11967; RRID: AB_2797784 Biological samples Fresh human blood New York Blood Center N/A Chemicals, peptides, and recombinant proteins Macrophage colony stimulating factor PeproTech Cat #315–02 Dopamine hydrochloride Sigma-Aldrich Cat #H8502 Lipopolysaccharide from Escherichia coli O55:B5 MilliporeSigma Cat #L2880 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Tocris Cat #0453 2′3′-cGAMP MedChemExpress Cat #HY-12512 Mdivi-1 MedChemExpress Cat #HY-15886 G-140 MedChemExpress Cat #HY-133916 H-151 MedChemExpress Cat #HY-112693 Ethidium bromide MedChemExpress Cat #HY-D0021 (Continued on next page) 20 Cell Reports 45, 118118, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BAY 11–7082 Selleck Chemicals Cat #S2913 JC-1 Thermo Fisher Scientific Cat #T3168 MitoSOXTM Red mitochondrial superoxide indicator Thermo Fisher Scientific Cat #M36008 Zombie NIRTM fixable viability dye BioLegend Cat #423105 SKF38393 Hydrochloride EMD Millipore Cat #505881 Quinpirole hydrochloride Tocris Cat #1061/10 Apomorphine hydrochloride Tocris Cat #2073/50 SCH23390 hydrochloride Tocris Cat #0925 Eticlopride hydrochloride Tocris Cat #1847 Accell green Non-targeting siRNA Horizon Discovery Cat #D-001950–01-05 Accell Human TMEM173 (340061) siRNA - SMARTpool Horizon Discovery Cat #E− 024333-00-0010 Critical commercial assays Pan Monocyte Isolation Kit Miltenyi Biotec Cat #130–096-537 AlphaLISA Human IL-6 Detection Kit Revvity Cat #AL223C MSD V-Plex Human Neuroinflammation Panel Meso Scale Diagnostics Cat #K15210D-1 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat #A55864 Human JAK/STAT Pathway Phosphorylation Array C1 RayBiotech Cat #AAH-JAKSTAT-1-8 XF Real-Time ATP Rate Assay Kit Agilent Cat #103592–100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat #103015–100 Mitochondria/Cytosol Fractionation Kit Abcam Cat #ab65320 Quick-DNA/RNATM Miniprep Kit Zymo Research Cat #D7001 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat #4368814 AlphaLISA SureFire Ultra Human Total STING Detection Kit Revvity Cat # ALSU-TSTNG-A10K Deposited data Mass spectrometry data ProteomeXchange Consortium; MassIVE repository PXD077267 Code for the principal component analysis and cluster analysis Zenodo https://doi.org/10.5281/ zenodo.19670069 Oligonucleotides DRD1 Thermo Fisher Scientific Cat #Hs00265245_s1 DRD2 Thermo Fisher Scientific Cat #Hs00241436_m1 DRD3 Thermo Fisher Scientific Cat #Hs00364455_m1 DRD4 Thermo Fisher Scientific Cat #Hs00609526_m1 DRD5 Thermo Fisher Scientific Cat #Hs00361234_s1 IFIT1 Thermo Fisher Scientific Cat #Hs01675197_m1 ISG15 Thermo Fisher Scientific Cat #Hs01921425_s1 18S Thermo Fisher Scientific Cat #4319413E Cytochrome c oxidase IDT DNA F: 5′GCCCCAGATATAGCATT CCC-3′ R: 5′-GTTCATCCTGTTCCTGC TCC-3′ Nuclear 18S IDT DNA F: 5′-TAGAGGGACAAGTGG CGTTC-3′ R: 5′CGCTGAGCCAG TCAGTGT-3′ Software and algorithms MSD Discovery Workbench Software version 4.0 Meso Scale Diagnostics N/A Image StudioTM Lite LI-COR Biosciences N/A DIA-NN (v2.2.0) N/A N/A ..

    Reverse Transcription:

    Article Title: Dopamine-induced mitochondrial dysfunction activates cGAS-sTING to drive IL-6 secretion in macrophages.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-DRP1 (Ser616) Rabbit Polyclonal Antibody Cell Signaling Technology Cat#3455; RRID: AB_2085352 DRP1 (D6C7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#8570; RRID: AB_10950498 cGAS (D1D3G) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#15102; RRID: AB_2732795 Phospho-STING (Ser366) (E9A9K) Cell Signaling Technology Cat#50907; RRID: AB_2827656 STING (D2P2F) Cell Signaling Technology Cat#13647; RRID: AB_2827656 phospho-TBK1 (Ser172) Polyclonal Antibody Thermo Fisher Scientific Cat #PA5-105919; RRID: AB_2817318 TBK1 Recombinant Rabbit Monoclonal Antibody (12H60L39) Thermo Fisher Scientific Cat #703154; RRID: AB_2848223 Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody (6D3D1) Thermo Fisher Scientific Cat #MA5-35850; RRID: AB_2849749 IRF3 Recombinant Rabbit Monoclonal Antibody (3H32L10) Thermo Fisher Scientific Cat #703682; RRID: AB_2784599 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling Technology Cat #4060; RRID: AB_2315049 Akt Antibody Cell Signaling Technology Cat #9272; RRID: AB_329827 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb Cell Signaling Technology Cat #4511; RRID: AB_2139682 Anti-p38 MAPK Antibody Cell Signaling Technology Cat #9212; RRID: AB_330713 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat #4370; RRID: AB_2315112 Mouse Anti-p44/42 MAP Kinase Monoclonal Antibody, Unconjugated, Clone L34F12 Cell Signaling Technology Cat #4696; RRID: AB_390780 Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb Cell Signaling Technology Cat #9323; RRID: AB_2115201 GSK-3beta (27C10) Rabbit mAb Cell Signaling Technology Cat #9315; RRID: AB_490890 Parkin (Prk8) Mouse monoclonal Antibody Cell Signaling Technology Cat #4211; RRID: AB_2159920 PINK1 (D8G3) Rabbit mAb Cell Signaling Technology Cat #6946; RRID: AB_11179069 Anti-BNIP3L/Nix (D4R4B) Rabbit Antibody Cell Signaling Technology Cat #12396; RRID: AB_2688036 LC3B (D11) XP Rabbit mAb Cell Signaling Technology Cat #3868; RRID: AB_2137707 NF-κB p65 (D14E12) XP Rabbit mAb Cell Signaling Technology Cat #8242; RRID: AB_10859369 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat #A-11008; RRID: AB_143165 LAMP1 (D2D11) XP Rabbit Antibody Cell Signaling Technology Cat #9091; RRID: AB_2687579 Recombinant Anti-TOMM20 antibody [EPR15581-39] Abcam Cat #ab289670; RRID: AB_2943038 Beta-Actin (13E5) Rabbit Monoclonal Antibody Cell Signaling Technology Cat #4970; RRID: AB_2223172 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat #11967; RRID: AB_2797784 Biological samples Fresh human blood New York Blood Center N/A Chemicals, peptides, and recombinant proteins Macrophage colony stimulating factor PeproTech Cat #315–02 Dopamine hydrochloride Sigma-Aldrich Cat #H8502 Lipopolysaccharide from Escherichia coli O55:B5 MilliporeSigma Cat #L2880 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Tocris Cat #0453 2′3′-cGAMP MedChemExpress Cat #HY-12512 Mdivi-1 MedChemExpress Cat #HY-15886 G-140 MedChemExpress Cat #HY-133916 H-151 MedChemExpress Cat #HY-112693 Ethidium bromide MedChemExpress Cat #HY-D0021 (Continued on next page) 20 Cell Reports 45, 118118, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BAY 11–7082 Selleck Chemicals Cat #S2913 JC-1 Thermo Fisher Scientific Cat #T3168 MitoSOXTM Red mitochondrial superoxide indicator Thermo Fisher Scientific Cat #M36008 Zombie NIRTM fixable viability dye BioLegend Cat #423105 SKF38393 Hydrochloride EMD Millipore Cat #505881 Quinpirole hydrochloride Tocris Cat #1061/10 Apomorphine hydrochloride Tocris Cat #2073/50 SCH23390 hydrochloride Tocris Cat #0925 Eticlopride hydrochloride Tocris Cat #1847 Accell green Non-targeting siRNA Horizon Discovery Cat #D-001950–01-05 Accell Human TMEM173 (340061) siRNA - SMARTpool Horizon Discovery Cat #E− 024333-00-0010 Critical commercial assays Pan Monocyte Isolation Kit Miltenyi Biotec Cat #130–096-537 AlphaLISA Human IL-6 Detection Kit Revvity Cat #AL223C MSD V-Plex Human Neuroinflammation Panel Meso Scale Diagnostics Cat #K15210D-1 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat #A55864 Human JAK/STAT Pathway Phosphorylation Array C1 RayBiotech Cat #AAH-JAKSTAT-1-8 XF Real-Time ATP Rate Assay Kit Agilent Cat #103592–100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat #103015–100 Mitochondria/Cytosol Fractionation Kit Abcam Cat #ab65320 Quick-DNA/RNATM Miniprep Kit Zymo Research Cat #D7001 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat #4368814 AlphaLISA SureFire Ultra Human Total STING Detection Kit Revvity Cat # ALSU-TSTNG-A10K Deposited data Mass spectrometry data ProteomeXchange Consortium; MassIVE repository PXD077267 Code for the principal component analysis and cluster analysis Zenodo https://doi.org/10.5281/ zenodo.19670069 Oligonucleotides DRD1 Thermo Fisher Scientific Cat #Hs00265245_s1 DRD2 Thermo Fisher Scientific Cat #Hs00241436_m1 DRD3 Thermo Fisher Scientific Cat #Hs00364455_m1 DRD4 Thermo Fisher Scientific Cat #Hs00609526_m1 DRD5 Thermo Fisher Scientific Cat #Hs00361234_s1 IFIT1 Thermo Fisher Scientific Cat #Hs01675197_m1 ISG15 Thermo Fisher Scientific Cat #Hs01921425_s1 18S Thermo Fisher Scientific Cat #4319413E Cytochrome c oxidase IDT DNA F: 5′GCCCCAGATATAGCATT CCC-3′ R: 5′-GTTCATCCTGTTCCTGC TCC-3′ Nuclear 18S IDT DNA F: 5′-TAGAGGGACAAGTGG CGTTC-3′ R: 5′CGCTGAGCCAG TCAGTGT-3′ Software and algorithms MSD Discovery Workbench Software version 4.0 Meso Scale Diagnostics N/A Image StudioTM Lite LI-COR Biosciences N/A DIA-NN (v2.2.0) N/A N/A ..

    Mass Spectrometry:

    Article Title: Dopamine-induced mitochondrial dysfunction activates cGAS-sTING to drive IL-6 secretion in macrophages.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-DRP1 (Ser616) Rabbit Polyclonal Antibody Cell Signaling Technology Cat#3455; RRID: AB_2085352 DRP1 (D6C7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#8570; RRID: AB_10950498 cGAS (D1D3G) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#15102; RRID: AB_2732795 Phospho-STING (Ser366) (E9A9K) Cell Signaling Technology Cat#50907; RRID: AB_2827656 STING (D2P2F) Cell Signaling Technology Cat#13647; RRID: AB_2827656 phospho-TBK1 (Ser172) Polyclonal Antibody Thermo Fisher Scientific Cat #PA5-105919; RRID: AB_2817318 TBK1 Recombinant Rabbit Monoclonal Antibody (12H60L39) Thermo Fisher Scientific Cat #703154; RRID: AB_2848223 Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody (6D3D1) Thermo Fisher Scientific Cat #MA5-35850; RRID: AB_2849749 IRF3 Recombinant Rabbit Monoclonal Antibody (3H32L10) Thermo Fisher Scientific Cat #703682; RRID: AB_2784599 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling Technology Cat #4060; RRID: AB_2315049 Akt Antibody Cell Signaling Technology Cat #9272; RRID: AB_329827 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb Cell Signaling Technology Cat #4511; RRID: AB_2139682 Anti-p38 MAPK Antibody Cell Signaling Technology Cat #9212; RRID: AB_330713 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat #4370; RRID: AB_2315112 Mouse Anti-p44/42 MAP Kinase Monoclonal Antibody, Unconjugated, Clone L34F12 Cell Signaling Technology Cat #4696; RRID: AB_390780 Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb Cell Signaling Technology Cat #9323; RRID: AB_2115201 GSK-3beta (27C10) Rabbit mAb Cell Signaling Technology Cat #9315; RRID: AB_490890 Parkin (Prk8) Mouse monoclonal Antibody Cell Signaling Technology Cat #4211; RRID: AB_2159920 PINK1 (D8G3) Rabbit mAb Cell Signaling Technology Cat #6946; RRID: AB_11179069 Anti-BNIP3L/Nix (D4R4B) Rabbit Antibody Cell Signaling Technology Cat #12396; RRID: AB_2688036 LC3B (D11) XP Rabbit mAb Cell Signaling Technology Cat #3868; RRID: AB_2137707 NF-κB p65 (D14E12) XP Rabbit mAb Cell Signaling Technology Cat #8242; RRID: AB_10859369 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat #A-11008; RRID: AB_143165 LAMP1 (D2D11) XP Rabbit Antibody Cell Signaling Technology Cat #9091; RRID: AB_2687579 Recombinant Anti-TOMM20 antibody [EPR15581-39] Abcam Cat #ab289670; RRID: AB_2943038 Beta-Actin (13E5) Rabbit Monoclonal Antibody Cell Signaling Technology Cat #4970; RRID: AB_2223172 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat #11967; RRID: AB_2797784 Biological samples Fresh human blood New York Blood Center N/A Chemicals, peptides, and recombinant proteins Macrophage colony stimulating factor PeproTech Cat #315–02 Dopamine hydrochloride Sigma-Aldrich Cat #H8502 Lipopolysaccharide from Escherichia coli O55:B5 MilliporeSigma Cat #L2880 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Tocris Cat #0453 2′3′-cGAMP MedChemExpress Cat #HY-12512 Mdivi-1 MedChemExpress Cat #HY-15886 G-140 MedChemExpress Cat #HY-133916 H-151 MedChemExpress Cat #HY-112693 Ethidium bromide MedChemExpress Cat #HY-D0021 (Continued on next page) 20 Cell Reports 45, 118118, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BAY 11–7082 Selleck Chemicals Cat #S2913 JC-1 Thermo Fisher Scientific Cat #T3168 MitoSOXTM Red mitochondrial superoxide indicator Thermo Fisher Scientific Cat #M36008 Zombie NIRTM fixable viability dye BioLegend Cat #423105 SKF38393 Hydrochloride EMD Millipore Cat #505881 Quinpirole hydrochloride Tocris Cat #1061/10 Apomorphine hydrochloride Tocris Cat #2073/50 SCH23390 hydrochloride Tocris Cat #0925 Eticlopride hydrochloride Tocris Cat #1847 Accell green Non-targeting siRNA Horizon Discovery Cat #D-001950–01-05 Accell Human TMEM173 (340061) siRNA - SMARTpool Horizon Discovery Cat #E− 024333-00-0010 Critical commercial assays Pan Monocyte Isolation Kit Miltenyi Biotec Cat #130–096-537 AlphaLISA Human IL-6 Detection Kit Revvity Cat #AL223C MSD V-Plex Human Neuroinflammation Panel Meso Scale Diagnostics Cat #K15210D-1 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat #A55864 Human JAK/STAT Pathway Phosphorylation Array C1 RayBiotech Cat #AAH-JAKSTAT-1-8 XF Real-Time ATP Rate Assay Kit Agilent Cat #103592–100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat #103015–100 Mitochondria/Cytosol Fractionation Kit Abcam Cat #ab65320 Quick-DNA/RNATM Miniprep Kit Zymo Research Cat #D7001 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat #4368814 AlphaLISA SureFire Ultra Human Total STING Detection Kit Revvity Cat # ALSU-TSTNG-A10K Deposited data Mass spectrometry data ProteomeXchange Consortium; MassIVE repository PXD077267 Code for the principal component analysis and cluster analysis Zenodo https://doi.org/10.5281/ zenodo.19670069 Oligonucleotides DRD1 Thermo Fisher Scientific Cat #Hs00265245_s1 DRD2 Thermo Fisher Scientific Cat #Hs00241436_m1 DRD3 Thermo Fisher Scientific Cat #Hs00364455_m1 DRD4 Thermo Fisher Scientific Cat #Hs00609526_m1 DRD5 Thermo Fisher Scientific Cat #Hs00361234_s1 IFIT1 Thermo Fisher Scientific Cat #Hs01675197_m1 ISG15 Thermo Fisher Scientific Cat #Hs01921425_s1 18S Thermo Fisher Scientific Cat #4319413E Cytochrome c oxidase IDT DNA F: 5′GCCCCAGATATAGCATT CCC-3′ R: 5′-GTTCATCCTGTTCCTGC TCC-3′ Nuclear 18S IDT DNA F: 5′-TAGAGGGACAAGTGG CGTTC-3′ R: 5′CGCTGAGCCAG TCAGTGT-3′ Software and algorithms MSD Discovery Workbench Software version 4.0 Meso Scale Diagnostics N/A Image StudioTM Lite LI-COR Biosciences N/A DIA-NN (v2.2.0) N/A N/A ..

    Analysis:

    Article Title: Dopamine-induced mitochondrial dysfunction activates cGAS-sTING to drive IL-6 secretion in macrophages.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-DRP1 (Ser616) Rabbit Polyclonal Antibody Cell Signaling Technology Cat#3455; RRID: AB_2085352 DRP1 (D6C7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#8570; RRID: AB_10950498 cGAS (D1D3G) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#15102; RRID: AB_2732795 Phospho-STING (Ser366) (E9A9K) Cell Signaling Technology Cat#50907; RRID: AB_2827656 STING (D2P2F) Cell Signaling Technology Cat#13647; RRID: AB_2827656 phospho-TBK1 (Ser172) Polyclonal Antibody Thermo Fisher Scientific Cat #PA5-105919; RRID: AB_2817318 TBK1 Recombinant Rabbit Monoclonal Antibody (12H60L39) Thermo Fisher Scientific Cat #703154; RRID: AB_2848223 Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody (6D3D1) Thermo Fisher Scientific Cat #MA5-35850; RRID: AB_2849749 IRF3 Recombinant Rabbit Monoclonal Antibody (3H32L10) Thermo Fisher Scientific Cat #703682; RRID: AB_2784599 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling Technology Cat #4060; RRID: AB_2315049 Akt Antibody Cell Signaling Technology Cat #9272; RRID: AB_329827 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb Cell Signaling Technology Cat #4511; RRID: AB_2139682 Anti-p38 MAPK Antibody Cell Signaling Technology Cat #9212; RRID: AB_330713 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat #4370; RRID: AB_2315112 Mouse Anti-p44/42 MAP Kinase Monoclonal Antibody, Unconjugated, Clone L34F12 Cell Signaling Technology Cat #4696; RRID: AB_390780 Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb Cell Signaling Technology Cat #9323; RRID: AB_2115201 GSK-3beta (27C10) Rabbit mAb Cell Signaling Technology Cat #9315; RRID: AB_490890 Parkin (Prk8) Mouse monoclonal Antibody Cell Signaling Technology Cat #4211; RRID: AB_2159920 PINK1 (D8G3) Rabbit mAb Cell Signaling Technology Cat #6946; RRID: AB_11179069 Anti-BNIP3L/Nix (D4R4B) Rabbit Antibody Cell Signaling Technology Cat #12396; RRID: AB_2688036 LC3B (D11) XP Rabbit mAb Cell Signaling Technology Cat #3868; RRID: AB_2137707 NF-κB p65 (D14E12) XP Rabbit mAb Cell Signaling Technology Cat #8242; RRID: AB_10859369 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat #A-11008; RRID: AB_143165 LAMP1 (D2D11) XP Rabbit Antibody Cell Signaling Technology Cat #9091; RRID: AB_2687579 Recombinant Anti-TOMM20 antibody [EPR15581-39] Abcam Cat #ab289670; RRID: AB_2943038 Beta-Actin (13E5) Rabbit Monoclonal Antibody Cell Signaling Technology Cat #4970; RRID: AB_2223172 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat #11967; RRID: AB_2797784 Biological samples Fresh human blood New York Blood Center N/A Chemicals, peptides, and recombinant proteins Macrophage colony stimulating factor PeproTech Cat #315–02 Dopamine hydrochloride Sigma-Aldrich Cat #H8502 Lipopolysaccharide from Escherichia coli O55:B5 MilliporeSigma Cat #L2880 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Tocris Cat #0453 2′3′-cGAMP MedChemExpress Cat #HY-12512 Mdivi-1 MedChemExpress Cat #HY-15886 G-140 MedChemExpress Cat #HY-133916 H-151 MedChemExpress Cat #HY-112693 Ethidium bromide MedChemExpress Cat #HY-D0021 (Continued on next page) 20 Cell Reports 45, 118118, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BAY 11–7082 Selleck Chemicals Cat #S2913 JC-1 Thermo Fisher Scientific Cat #T3168 MitoSOXTM Red mitochondrial superoxide indicator Thermo Fisher Scientific Cat #M36008 Zombie NIRTM fixable viability dye BioLegend Cat #423105 SKF38393 Hydrochloride EMD Millipore Cat #505881 Quinpirole hydrochloride Tocris Cat #1061/10 Apomorphine hydrochloride Tocris Cat #2073/50 SCH23390 hydrochloride Tocris Cat #0925 Eticlopride hydrochloride Tocris Cat #1847 Accell green Non-targeting siRNA Horizon Discovery Cat #D-001950–01-05 Accell Human TMEM173 (340061) siRNA - SMARTpool Horizon Discovery Cat #E− 024333-00-0010 Critical commercial assays Pan Monocyte Isolation Kit Miltenyi Biotec Cat #130–096-537 AlphaLISA Human IL-6 Detection Kit Revvity Cat #AL223C MSD V-Plex Human Neuroinflammation Panel Meso Scale Diagnostics Cat #K15210D-1 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat #A55864 Human JAK/STAT Pathway Phosphorylation Array C1 RayBiotech Cat #AAH-JAKSTAT-1-8 XF Real-Time ATP Rate Assay Kit Agilent Cat #103592–100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat #103015–100 Mitochondria/Cytosol Fractionation Kit Abcam Cat #ab65320 Quick-DNA/RNATM Miniprep Kit Zymo Research Cat #D7001 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat #4368814 AlphaLISA SureFire Ultra Human Total STING Detection Kit Revvity Cat # ALSU-TSTNG-A10K Deposited data Mass spectrometry data ProteomeXchange Consortium; MassIVE repository PXD077267 Code for the principal component analysis and cluster analysis Zenodo https://doi.org/10.5281/ zenodo.19670069 Oligonucleotides DRD1 Thermo Fisher Scientific Cat #Hs00265245_s1 DRD2 Thermo Fisher Scientific Cat #Hs00241436_m1 DRD3 Thermo Fisher Scientific Cat #Hs00364455_m1 DRD4 Thermo Fisher Scientific Cat #Hs00609526_m1 DRD5 Thermo Fisher Scientific Cat #Hs00361234_s1 IFIT1 Thermo Fisher Scientific Cat #Hs01675197_m1 ISG15 Thermo Fisher Scientific Cat #Hs01921425_s1 18S Thermo Fisher Scientific Cat #4319413E Cytochrome c oxidase IDT DNA F: 5′GCCCCAGATATAGCATT CCC-3′ R: 5′-GTTCATCCTGTTCCTGC TCC-3′ Nuclear 18S IDT DNA F: 5′-TAGAGGGACAAGTGG CGTTC-3′ R: 5′CGCTGAGCCAG TCAGTGT-3′ Software and algorithms MSD Discovery Workbench Software version 4.0 Meso Scale Diagnostics N/A Image StudioTM Lite LI-COR Biosciences N/A DIA-NN (v2.2.0) N/A N/A ..

    Countercurrent Chromatography:

    Article Title: Dopamine-induced mitochondrial dysfunction activates cGAS-sTING to drive IL-6 secretion in macrophages.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-DRP1 (Ser616) Rabbit Polyclonal Antibody Cell Signaling Technology Cat#3455; RRID: AB_2085352 DRP1 (D6C7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#8570; RRID: AB_10950498 cGAS (D1D3G) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#15102; RRID: AB_2732795 Phospho-STING (Ser366) (E9A9K) Cell Signaling Technology Cat#50907; RRID: AB_2827656 STING (D2P2F) Cell Signaling Technology Cat#13647; RRID: AB_2827656 phospho-TBK1 (Ser172) Polyclonal Antibody Thermo Fisher Scientific Cat #PA5-105919; RRID: AB_2817318 TBK1 Recombinant Rabbit Monoclonal Antibody (12H60L39) Thermo Fisher Scientific Cat #703154; RRID: AB_2848223 Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody (6D3D1) Thermo Fisher Scientific Cat #MA5-35850; RRID: AB_2849749 IRF3 Recombinant Rabbit Monoclonal Antibody (3H32L10) Thermo Fisher Scientific Cat #703682; RRID: AB_2784599 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling Technology Cat #4060; RRID: AB_2315049 Akt Antibody Cell Signaling Technology Cat #9272; RRID: AB_329827 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb Cell Signaling Technology Cat #4511; RRID: AB_2139682 Anti-p38 MAPK Antibody Cell Signaling Technology Cat #9212; RRID: AB_330713 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat #4370; RRID: AB_2315112 Mouse Anti-p44/42 MAP Kinase Monoclonal Antibody, Unconjugated, Clone L34F12 Cell Signaling Technology Cat #4696; RRID: AB_390780 Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb Cell Signaling Technology Cat #9323; RRID: AB_2115201 GSK-3beta (27C10) Rabbit mAb Cell Signaling Technology Cat #9315; RRID: AB_490890 Parkin (Prk8) Mouse monoclonal Antibody Cell Signaling Technology Cat #4211; RRID: AB_2159920 PINK1 (D8G3) Rabbit mAb Cell Signaling Technology Cat #6946; RRID: AB_11179069 Anti-BNIP3L/Nix (D4R4B) Rabbit Antibody Cell Signaling Technology Cat #12396; RRID: AB_2688036 LC3B (D11) XP Rabbit mAb Cell Signaling Technology Cat #3868; RRID: AB_2137707 NF-κB p65 (D14E12) XP Rabbit mAb Cell Signaling Technology Cat #8242; RRID: AB_10859369 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat #A-11008; RRID: AB_143165 LAMP1 (D2D11) XP Rabbit Antibody Cell Signaling Technology Cat #9091; RRID: AB_2687579 Recombinant Anti-TOMM20 antibody [EPR15581-39] Abcam Cat #ab289670; RRID: AB_2943038 Beta-Actin (13E5) Rabbit Monoclonal Antibody Cell Signaling Technology Cat #4970; RRID: AB_2223172 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat #11967; RRID: AB_2797784 Biological samples Fresh human blood New York Blood Center N/A Chemicals, peptides, and recombinant proteins Macrophage colony stimulating factor PeproTech Cat #315–02 Dopamine hydrochloride Sigma-Aldrich Cat #H8502 Lipopolysaccharide from Escherichia coli O55:B5 MilliporeSigma Cat #L2880 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Tocris Cat #0453 2′3′-cGAMP MedChemExpress Cat #HY-12512 Mdivi-1 MedChemExpress Cat #HY-15886 G-140 MedChemExpress Cat #HY-133916 H-151 MedChemExpress Cat #HY-112693 Ethidium bromide MedChemExpress Cat #HY-D0021 (Continued on next page) 20 Cell Reports 45, 118118, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BAY 11–7082 Selleck Chemicals Cat #S2913 JC-1 Thermo Fisher Scientific Cat #T3168 MitoSOXTM Red mitochondrial superoxide indicator Thermo Fisher Scientific Cat #M36008 Zombie NIRTM fixable viability dye BioLegend Cat #423105 SKF38393 Hydrochloride EMD Millipore Cat #505881 Quinpirole hydrochloride Tocris Cat #1061/10 Apomorphine hydrochloride Tocris Cat #2073/50 SCH23390 hydrochloride Tocris Cat #0925 Eticlopride hydrochloride Tocris Cat #1847 Accell green Non-targeting siRNA Horizon Discovery Cat #D-001950–01-05 Accell Human TMEM173 (340061) siRNA - SMARTpool Horizon Discovery Cat #E− 024333-00-0010 Critical commercial assays Pan Monocyte Isolation Kit Miltenyi Biotec Cat #130–096-537 AlphaLISA Human IL-6 Detection Kit Revvity Cat #AL223C MSD V-Plex Human Neuroinflammation Panel Meso Scale Diagnostics Cat #K15210D-1 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat #A55864 Human JAK/STAT Pathway Phosphorylation Array C1 RayBiotech Cat #AAH-JAKSTAT-1-8 XF Real-Time ATP Rate Assay Kit Agilent Cat #103592–100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat #103015–100 Mitochondria/Cytosol Fractionation Kit Abcam Cat #ab65320 Quick-DNA/RNATM Miniprep Kit Zymo Research Cat #D7001 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat #4368814 AlphaLISA SureFire Ultra Human Total STING Detection Kit Revvity Cat # ALSU-TSTNG-A10K Deposited data Mass spectrometry data ProteomeXchange Consortium; MassIVE repository PXD077267 Code for the principal component analysis and cluster analysis Zenodo https://doi.org/10.5281/ zenodo.19670069 Oligonucleotides DRD1 Thermo Fisher Scientific Cat #Hs00265245_s1 DRD2 Thermo Fisher Scientific Cat #Hs00241436_m1 DRD3 Thermo Fisher Scientific Cat #Hs00364455_m1 DRD4 Thermo Fisher Scientific Cat #Hs00609526_m1 DRD5 Thermo Fisher Scientific Cat #Hs00361234_s1 IFIT1 Thermo Fisher Scientific Cat #Hs01675197_m1 ISG15 Thermo Fisher Scientific Cat #Hs01921425_s1 18S Thermo Fisher Scientific Cat #4319413E Cytochrome c oxidase IDT DNA F: 5′GCCCCAGATATAGCATT CCC-3′ R: 5′-GTTCATCCTGTTCCTGC TCC-3′ Nuclear 18S IDT DNA F: 5′-TAGAGGGACAAGTGG CGTTC-3′ R: 5′CGCTGAGCCAG TCAGTGT-3′ Software and algorithms MSD Discovery Workbench Software version 4.0 Meso Scale Diagnostics N/A Image StudioTM Lite LI-COR Biosciences N/A DIA-NN (v2.2.0) N/A N/A ..

    Software:

    Article Title: Dopamine-induced mitochondrial dysfunction activates cGAS-sTING to drive IL-6 secretion in macrophages.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-DRP1 (Ser616) Rabbit Polyclonal Antibody Cell Signaling Technology Cat#3455; RRID: AB_2085352 DRP1 (D6C7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#8570; RRID: AB_10950498 cGAS (D1D3G) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#15102; RRID: AB_2732795 Phospho-STING (Ser366) (E9A9K) Cell Signaling Technology Cat#50907; RRID: AB_2827656 STING (D2P2F) Cell Signaling Technology Cat#13647; RRID: AB_2827656 phospho-TBK1 (Ser172) Polyclonal Antibody Thermo Fisher Scientific Cat #PA5-105919; RRID: AB_2817318 TBK1 Recombinant Rabbit Monoclonal Antibody (12H60L39) Thermo Fisher Scientific Cat #703154; RRID: AB_2848223 Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody (6D3D1) Thermo Fisher Scientific Cat #MA5-35850; RRID: AB_2849749 IRF3 Recombinant Rabbit Monoclonal Antibody (3H32L10) Thermo Fisher Scientific Cat #703682; RRID: AB_2784599 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling Technology Cat #4060; RRID: AB_2315049 Akt Antibody Cell Signaling Technology Cat #9272; RRID: AB_329827 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb Cell Signaling Technology Cat #4511; RRID: AB_2139682 Anti-p38 MAPK Antibody Cell Signaling Technology Cat #9212; RRID: AB_330713 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat #4370; RRID: AB_2315112 Mouse Anti-p44/42 MAP Kinase Monoclonal Antibody, Unconjugated, Clone L34F12 Cell Signaling Technology Cat #4696; RRID: AB_390780 Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb Cell Signaling Technology Cat #9323; RRID: AB_2115201 GSK-3beta (27C10) Rabbit mAb Cell Signaling Technology Cat #9315; RRID: AB_490890 Parkin (Prk8) Mouse monoclonal Antibody Cell Signaling Technology Cat #4211; RRID: AB_2159920 PINK1 (D8G3) Rabbit mAb Cell Signaling Technology Cat #6946; RRID: AB_11179069 Anti-BNIP3L/Nix (D4R4B) Rabbit Antibody Cell Signaling Technology Cat #12396; RRID: AB_2688036 LC3B (D11) XP Rabbit mAb Cell Signaling Technology Cat #3868; RRID: AB_2137707 NF-κB p65 (D14E12) XP Rabbit mAb Cell Signaling Technology Cat #8242; RRID: AB_10859369 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat #A-11008; RRID: AB_143165 LAMP1 (D2D11) XP Rabbit Antibody Cell Signaling Technology Cat #9091; RRID: AB_2687579 Recombinant Anti-TOMM20 antibody [EPR15581-39] Abcam Cat #ab289670; RRID: AB_2943038 Beta-Actin (13E5) Rabbit Monoclonal Antibody Cell Signaling Technology Cat #4970; RRID: AB_2223172 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat #11967; RRID: AB_2797784 Biological samples Fresh human blood New York Blood Center N/A Chemicals, peptides, and recombinant proteins Macrophage colony stimulating factor PeproTech Cat #315–02 Dopamine hydrochloride Sigma-Aldrich Cat #H8502 Lipopolysaccharide from Escherichia coli O55:B5 MilliporeSigma Cat #L2880 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Tocris Cat #0453 2′3′-cGAMP MedChemExpress Cat #HY-12512 Mdivi-1 MedChemExpress Cat #HY-15886 G-140 MedChemExpress Cat #HY-133916 H-151 MedChemExpress Cat #HY-112693 Ethidium bromide MedChemExpress Cat #HY-D0021 (Continued on next page) 20 Cell Reports 45, 118118, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BAY 11–7082 Selleck Chemicals Cat #S2913 JC-1 Thermo Fisher Scientific Cat #T3168 MitoSOXTM Red mitochondrial superoxide indicator Thermo Fisher Scientific Cat #M36008 Zombie NIRTM fixable viability dye BioLegend Cat #423105 SKF38393 Hydrochloride EMD Millipore Cat #505881 Quinpirole hydrochloride Tocris Cat #1061/10 Apomorphine hydrochloride Tocris Cat #2073/50 SCH23390 hydrochloride Tocris Cat #0925 Eticlopride hydrochloride Tocris Cat #1847 Accell green Non-targeting siRNA Horizon Discovery Cat #D-001950–01-05 Accell Human TMEM173 (340061) siRNA - SMARTpool Horizon Discovery Cat #E− 024333-00-0010 Critical commercial assays Pan Monocyte Isolation Kit Miltenyi Biotec Cat #130–096-537 AlphaLISA Human IL-6 Detection Kit Revvity Cat #AL223C MSD V-Plex Human Neuroinflammation Panel Meso Scale Diagnostics Cat #K15210D-1 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat #A55864 Human JAK/STAT Pathway Phosphorylation Array C1 RayBiotech Cat #AAH-JAKSTAT-1-8 XF Real-Time ATP Rate Assay Kit Agilent Cat #103592–100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat #103015–100 Mitochondria/Cytosol Fractionation Kit Abcam Cat #ab65320 Quick-DNA/RNATM Miniprep Kit Zymo Research Cat #D7001 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat #4368814 AlphaLISA SureFire Ultra Human Total STING Detection Kit Revvity Cat # ALSU-TSTNG-A10K Deposited data Mass spectrometry data ProteomeXchange Consortium; MassIVE repository PXD077267 Code for the principal component analysis and cluster analysis Zenodo https://doi.org/10.5281/ zenodo.19670069 Oligonucleotides DRD1 Thermo Fisher Scientific Cat #Hs00265245_s1 DRD2 Thermo Fisher Scientific Cat #Hs00241436_m1 DRD3 Thermo Fisher Scientific Cat #Hs00364455_m1 DRD4 Thermo Fisher Scientific Cat #Hs00609526_m1 DRD5 Thermo Fisher Scientific Cat #Hs00361234_s1 IFIT1 Thermo Fisher Scientific Cat #Hs01675197_m1 ISG15 Thermo Fisher Scientific Cat #Hs01921425_s1 18S Thermo Fisher Scientific Cat #4319413E Cytochrome c oxidase IDT DNA F: 5′GCCCCAGATATAGCATT CCC-3′ R: 5′-GTTCATCCTGTTCCTGC TCC-3′ Nuclear 18S IDT DNA F: 5′-TAGAGGGACAAGTGG CGTTC-3′ R: 5′CGCTGAGCCAG TCAGTGT-3′ Software and algorithms MSD Discovery Workbench Software version 4.0 Meso Scale Diagnostics N/A Image StudioTM Lite LI-COR Biosciences N/A DIA-NN (v2.2.0) N/A N/A ..

    Data-independent acquisition:

    Article Title: Dopamine-induced mitochondrial dysfunction activates cGAS-sTING to drive IL-6 secretion in macrophages.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-DRP1 (Ser616) Rabbit Polyclonal Antibody Cell Signaling Technology Cat#3455; RRID: AB_2085352 DRP1 (D6C7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#8570; RRID: AB_10950498 cGAS (D1D3G) Rabbit Monoclonal Antibody Cell Signaling Technology Cat#15102; RRID: AB_2732795 Phospho-STING (Ser366) (E9A9K) Cell Signaling Technology Cat#50907; RRID: AB_2827656 STING (D2P2F) Cell Signaling Technology Cat#13647; RRID: AB_2827656 phospho-TBK1 (Ser172) Polyclonal Antibody Thermo Fisher Scientific Cat #PA5-105919; RRID: AB_2817318 TBK1 Recombinant Rabbit Monoclonal Antibody (12H60L39) Thermo Fisher Scientific Cat #703154; RRID: AB_2848223 Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody (6D3D1) Thermo Fisher Scientific Cat #MA5-35850; RRID: AB_2849749 IRF3 Recombinant Rabbit Monoclonal Antibody (3H32L10) Thermo Fisher Scientific Cat #703682; RRID: AB_2784599 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling Technology Cat #4060; RRID: AB_2315049 Akt Antibody Cell Signaling Technology Cat #9272; RRID: AB_329827 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb Cell Signaling Technology Cat #4511; RRID: AB_2139682 Anti-p38 MAPK Antibody Cell Signaling Technology Cat #9212; RRID: AB_330713 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat #4370; RRID: AB_2315112 Mouse Anti-p44/42 MAP Kinase Monoclonal Antibody, Unconjugated, Clone L34F12 Cell Signaling Technology Cat #4696; RRID: AB_390780 Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb Cell Signaling Technology Cat #9323; RRID: AB_2115201 GSK-3beta (27C10) Rabbit mAb Cell Signaling Technology Cat #9315; RRID: AB_490890 Parkin (Prk8) Mouse monoclonal Antibody Cell Signaling Technology Cat #4211; RRID: AB_2159920 PINK1 (D8G3) Rabbit mAb Cell Signaling Technology Cat #6946; RRID: AB_11179069 Anti-BNIP3L/Nix (D4R4B) Rabbit Antibody Cell Signaling Technology Cat #12396; RRID: AB_2688036 LC3B (D11) XP Rabbit mAb Cell Signaling Technology Cat #3868; RRID: AB_2137707 NF-κB p65 (D14E12) XP Rabbit mAb Cell Signaling Technology Cat #8242; RRID: AB_10859369 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat #A-11008; RRID: AB_143165 LAMP1 (D2D11) XP Rabbit Antibody Cell Signaling Technology Cat #9091; RRID: AB_2687579 Recombinant Anti-TOMM20 antibody [EPR15581-39] Abcam Cat #ab289670; RRID: AB_2943038 Beta-Actin (13E5) Rabbit Monoclonal Antibody Cell Signaling Technology Cat #4970; RRID: AB_2223172 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat #11967; RRID: AB_2797784 Biological samples Fresh human blood New York Blood Center N/A Chemicals, peptides, and recombinant proteins Macrophage colony stimulating factor PeproTech Cat #315–02 Dopamine hydrochloride Sigma-Aldrich Cat #H8502 Lipopolysaccharide from Escherichia coli O55:B5 MilliporeSigma Cat #L2880 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Tocris Cat #0453 2′3′-cGAMP MedChemExpress Cat #HY-12512 Mdivi-1 MedChemExpress Cat #HY-15886 G-140 MedChemExpress Cat #HY-133916 H-151 MedChemExpress Cat #HY-112693 Ethidium bromide MedChemExpress Cat #HY-D0021 (Continued on next page) 20 Cell Reports 45, 118118, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BAY 11–7082 Selleck Chemicals Cat #S2913 JC-1 Thermo Fisher Scientific Cat #T3168 MitoSOXTM Red mitochondrial superoxide indicator Thermo Fisher Scientific Cat #M36008 Zombie NIRTM fixable viability dye BioLegend Cat #423105 SKF38393 Hydrochloride EMD Millipore Cat #505881 Quinpirole hydrochloride Tocris Cat #1061/10 Apomorphine hydrochloride Tocris Cat #2073/50 SCH23390 hydrochloride Tocris Cat #0925 Eticlopride hydrochloride Tocris Cat #1847 Accell green Non-targeting siRNA Horizon Discovery Cat #D-001950–01-05 Accell Human TMEM173 (340061) siRNA - SMARTpool Horizon Discovery Cat #E− 024333-00-0010 Critical commercial assays Pan Monocyte Isolation Kit Miltenyi Biotec Cat #130–096-537 AlphaLISA Human IL-6 Detection Kit Revvity Cat #AL223C MSD V-Plex Human Neuroinflammation Panel Meso Scale Diagnostics Cat #K15210D-1 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat #A55864 Human JAK/STAT Pathway Phosphorylation Array C1 RayBiotech Cat #AAH-JAKSTAT-1-8 XF Real-Time ATP Rate Assay Kit Agilent Cat #103592–100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat #103015–100 Mitochondria/Cytosol Fractionation Kit Abcam Cat #ab65320 Quick-DNA/RNATM Miniprep Kit Zymo Research Cat #D7001 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat #4368814 AlphaLISA SureFire Ultra Human Total STING Detection Kit Revvity Cat # ALSU-TSTNG-A10K Deposited data Mass spectrometry data ProteomeXchange Consortium; MassIVE repository PXD077267 Code for the principal component analysis and cluster analysis Zenodo https://doi.org/10.5281/ zenodo.19670069 Oligonucleotides DRD1 Thermo Fisher Scientific Cat #Hs00265245_s1 DRD2 Thermo Fisher Scientific Cat #Hs00241436_m1 DRD3 Thermo Fisher Scientific Cat #Hs00364455_m1 DRD4 Thermo Fisher Scientific Cat #Hs00609526_m1 DRD5 Thermo Fisher Scientific Cat #Hs00361234_s1 IFIT1 Thermo Fisher Scientific Cat #Hs01675197_m1 ISG15 Thermo Fisher Scientific Cat #Hs01921425_s1 18S Thermo Fisher Scientific Cat #4319413E Cytochrome c oxidase IDT DNA F: 5′GCCCCAGATATAGCATT CCC-3′ R: 5′-GTTCATCCTGTTCCTGC TCC-3′ Nuclear 18S IDT DNA F: 5′-TAGAGGGACAAGTGG CGTTC-3′ R: 5′CGCTGAGCCAG TCAGTGT-3′ Software and algorithms MSD Discovery Workbench Software version 4.0 Meso Scale Diagnostics N/A Image StudioTM Lite LI-COR Biosciences N/A DIA-NN (v2.2.0) N/A N/A ..



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