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purelink mirna isolation kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher purelink mirna isolation kit
    Purelink Mirna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolation+kit/nct04488614-6-7-11
    Average 86 stars, based on 1 article reviews
    purelink mirna isolation kit - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    other:

    Article Title: Prospective Breast Cancer Biobanking Project
    Article Snippet: MiRNA and mRNA will be profiled using PureLinkTM miRNA Isolation Kit (Invitrogen), Dynabeads mRNA direct micro kit (Ambion),) and Ion total RNA-seq kit V2 (Life Technologies) on our Ion Proton NGS instrument.

    Article Title: Inhibition of iRhom1 by CD44-targeting nanocarrier for improved cancer immunochemotherapy.
    Article Snippet: The RNAwas further purified with PureLinkTM miRNA Isolation Kit (Invitrogen, NY, U.S.A).

    Article Title: Inhibition of iRhom1 by CD44-targeting nanocarrier for improved cancer immunochemotherapy
    Article Snippet: The RNA was further purified with PureLinkTM miRNA Isolation Kit (Invitrogen, NY, U.S.A).

    Isolation:

    Article Title: Decoding Codon Bias: The Role of tRNA Modifications in Tissue-Specific Translation
    Article Snippet: .. Tissues were homogenized with QIAzol (QIAGEN, Hilden, Germany, #79306) and small RNA was isolated using a Purelink miRNA Isolation Kit (Thermo Fisher, Waltham, MA, USA, #K157001). .. For small RNA sequencing and ribosome profiling, three mice were perfused using ice-cold phosphate-buffered saline with 100 μg/mL cycloheximide (Sigma, St. Lousi, MO, USA, #01810).

    Article Title: Synergistic effects of tRNA modification defects in Escherichia coli K12
    Article Snippet: .. Pellets were resuspended in 1 mL of Trizol (Life Technologies) and agitated on a rocking shaker for 2-4 h. Small RNA was extracted using a Purelink miRNA Isolation kit (Invitrogen) and RNA eluted in 50 μL of RNase-free water. .. Following RNA quantification by Nanodrop, samples with A 260 /A 230 ratios <1.6 were cleaned using an RNA Clean and Concentrator Kit (Norgen Biotek).

    Article Title: A Novel tsRNA, m 7 G‐3′ tiRNA Lys TTT , Promotes Bladder Cancer Malignancy Via Regulating ANXA2 Phosphorylation
    Article Snippet: .. As previously described, [ ] small RNA was extracted using a PureLink miRNA isolation kit (Invitrogen). tiRNAs were purified on 15% TBE‐urea polyacrylamide gels and soaked in elution buffer, followed by hybridization with biotinylated oligonucleotide DNA probes complementary to the target tiRNAs. .. Then the biotinylated oligo‐tiRNA complex was bound to Streptavidin Magnetic Beads (Thermo Scientific, 88 816) and extracted using trziol regent.

    Article Title: The ubiquitous pyridoxal 5′‐phosphate‐binding protein is also an RNA ‐binding protein
    Article Snippet: Total RNA was isolated from BW25113 E. coli or human MDA‐MB‐231 and HAP1 cell cultures using the NucleoSpin RNA kit from Macherey and Nagel (Hoerdt, France). .. Low molecular weight RNA was purified from BW25113 E. coli cells using PureLink miRNA Isolation kit (Invitrogen, Waltham, MA, USA). .. RNA bound to proteins was purified using a total RNA purification kit from Norgen Biotek (Thorold, Ontario, Canada).

    Article Title: Mechanism of Catalysis and Substrate Binding of Epoxyqueuosine Reductase in the Biosynthetic Pathway to Queuosine-Modified tRNA.
    Article Snippet: Post-transcriptional modifications at the anticodon stem-loop of tRNAs are key to the translation function.. Metabolic pathways to these modifications often incorporate complex enzymology.. A notable example is the hypermodified nucleoside, queuosine, found at the wobble position of Asn, Asp, His, and Tyr encoding tRNAs.

    Purification:

    Article Title: A Novel tsRNA, m 7 G‐3′ tiRNA Lys TTT , Promotes Bladder Cancer Malignancy Via Regulating ANXA2 Phosphorylation
    Article Snippet: .. As previously described, [ ] small RNA was extracted using a PureLink miRNA isolation kit (Invitrogen). tiRNAs were purified on 15% TBE‐urea polyacrylamide gels and soaked in elution buffer, followed by hybridization with biotinylated oligonucleotide DNA probes complementary to the target tiRNAs. .. Then the biotinylated oligo‐tiRNA complex was bound to Streptavidin Magnetic Beads (Thermo Scientific, 88 816) and extracted using trziol regent.

    Article Title: The ubiquitous pyridoxal 5′‐phosphate‐binding protein is also an RNA ‐binding protein
    Article Snippet: Total RNA was isolated from BW25113 E. coli or human MDA‐MB‐231 and HAP1 cell cultures using the NucleoSpin RNA kit from Macherey and Nagel (Hoerdt, France). .. Low molecular weight RNA was purified from BW25113 E. coli cells using PureLink miRNA Isolation kit (Invitrogen, Waltham, MA, USA). .. RNA bound to proteins was purified using a total RNA purification kit from Norgen Biotek (Thorold, Ontario, Canada).

    Hybridization:

    Article Title: A Novel tsRNA, m 7 G‐3′ tiRNA Lys TTT , Promotes Bladder Cancer Malignancy Via Regulating ANXA2 Phosphorylation
    Article Snippet: .. As previously described, [ ] small RNA was extracted using a PureLink miRNA isolation kit (Invitrogen). tiRNAs were purified on 15% TBE‐urea polyacrylamide gels and soaked in elution buffer, followed by hybridization with biotinylated oligonucleotide DNA probes complementary to the target tiRNAs. .. Then the biotinylated oligo‐tiRNA complex was bound to Streptavidin Magnetic Beads (Thermo Scientific, 88 816) and extracted using trziol regent.

    Molecular Weight:

    Article Title: The ubiquitous pyridoxal 5′‐phosphate‐binding protein is also an RNA ‐binding protein
    Article Snippet: Total RNA was isolated from BW25113 E. coli or human MDA‐MB‐231 and HAP1 cell cultures using the NucleoSpin RNA kit from Macherey and Nagel (Hoerdt, France). .. Low molecular weight RNA was purified from BW25113 E. coli cells using PureLink miRNA Isolation kit (Invitrogen, Waltham, MA, USA). .. RNA bound to proteins was purified using a total RNA purification kit from Norgen Biotek (Thorold, Ontario, Canada).



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    Image Search Results


    Increased expression of desmin and vimentin does not cause mitochondrial structural abnormalities. (A) Human BSM cells were transduced with an adenovirus encoding desmin and vimentin, and GFP for 36 h. The expression levels of desmin and vimentin proteins were analyzed by immunoblotting following their overexpression. GAPDH served as a loading control. Representative immunoblots and corresponding quantifications are shown. (B–D) The expression levels of contractile proteins and desmin and vimentin proteins across passages (P3–P5) were analyzed by immunoblotting. GAPDH served as a loading control. Representative immunoblots and their corresponding quantifications are shown in panels B, C, and D. (E) TEM imaging : Human BSM cells overexpressing desmin and vimentin were fixed and processed for TEM. GFP‐expressing cells were used as a control group. The TEM images in panel E depict mitochondria from lower (upper panel) and higher (lower panel) magnifications. Scale bars = 500 nm. M denotes molecular weight marker. Data are presented as mean ± SD; n = 3–4 in A, n = 3 in B–D, and n = 2 in E. The ns indicates non‐significant. **** p < 0.0001.

    Journal: The FASEB Journal

    Article Title: Increased Expression of Desmin and Vimentin Impairs Mitochondrial Function and Reduces Contractility via G0S2 in Bladder Smooth Muscle

    doi: 10.1096/fj.202502912R

    Figure Lengend Snippet: Increased expression of desmin and vimentin does not cause mitochondrial structural abnormalities. (A) Human BSM cells were transduced with an adenovirus encoding desmin and vimentin, and GFP for 36 h. The expression levels of desmin and vimentin proteins were analyzed by immunoblotting following their overexpression. GAPDH served as a loading control. Representative immunoblots and corresponding quantifications are shown. (B–D) The expression levels of contractile proteins and desmin and vimentin proteins across passages (P3–P5) were analyzed by immunoblotting. GAPDH served as a loading control. Representative immunoblots and their corresponding quantifications are shown in panels B, C, and D. (E) TEM imaging : Human BSM cells overexpressing desmin and vimentin were fixed and processed for TEM. GFP‐expressing cells were used as a control group. The TEM images in panel E depict mitochondria from lower (upper panel) and higher (lower panel) magnifications. Scale bars = 500 nm. M denotes molecular weight marker. Data are presented as mean ± SD; n = 3–4 in A, n = 3 in B–D, and n = 2 in E. The ns indicates non‐significant. **** p < 0.0001.

    Article Snippet: Mitochondria were isolated from human BSM cells and murine BSM tissue using the Mitochondria Isolation Kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer's instructions.

    Techniques: Expressing, Transduction, Western Blot, Over Expression, Control, Imaging, Molecular Weight, Marker

    Increased expression of desmin and vimentin reduces mitochondrial elongation and branching. (A) Human BSM cells expressing DsRed2‐Mito‐7 were transduced with adenovirus encoding GFP, desmin, and vimentin for 36 h, and the mitochondria in these cells were visualized via confocal microscopy. GFP‐expressing cells served as the control group. Confocal images of mitochondria were processed using Python‐based pipelines, as described in the Materials and Methods section. Representative confocal images are shown in panel A. The white color in panel A represents mitochondria. (B–F) Quantification of mitochondrial length and circularity, represented by aspect ratio and form factor, respectively. The quantification of the branching parameter is shown in panels D–F. Scale bars = 50 μm. Data are presented as mean ± SD; n > 75 cells per group. Ns indicates non‐significant. *** p < 0.001 and **** p < 0.0001.

    Journal: The FASEB Journal

    Article Title: Increased Expression of Desmin and Vimentin Impairs Mitochondrial Function and Reduces Contractility via G0S2 in Bladder Smooth Muscle

    doi: 10.1096/fj.202502912R

    Figure Lengend Snippet: Increased expression of desmin and vimentin reduces mitochondrial elongation and branching. (A) Human BSM cells expressing DsRed2‐Mito‐7 were transduced with adenovirus encoding GFP, desmin, and vimentin for 36 h, and the mitochondria in these cells were visualized via confocal microscopy. GFP‐expressing cells served as the control group. Confocal images of mitochondria were processed using Python‐based pipelines, as described in the Materials and Methods section. Representative confocal images are shown in panel A. The white color in panel A represents mitochondria. (B–F) Quantification of mitochondrial length and circularity, represented by aspect ratio and form factor, respectively. The quantification of the branching parameter is shown in panels D–F. Scale bars = 50 μm. Data are presented as mean ± SD; n > 75 cells per group. Ns indicates non‐significant. *** p < 0.001 and **** p < 0.0001.

    Article Snippet: Mitochondria were isolated from human BSM cells and murine BSM tissue using the Mitochondria Isolation Kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer's instructions.

    Techniques: Expressing, Transduction, Confocal, Microscopy, Control

    Increased expression of desmin and vimentin enhances mitochondrial ROS and reduces oxygen consumption rate and ATP levels. Human BSM cells were transduced with an adenovirus encoding desmin and vimentin, and GFP for 36 h. Human BSM cells overexpressing desmin and vimentin were processed for ROS, OCR, and ATP measurements. GFP‐expressing cells served as a control group. (A) Mitochondrial ROS levels in live cells were assessed by MitoSOX fluorescence. Human BSM cells overexpressing desmin and vimentin were stained with MitoSOX (5 μM) and MitoTracker DeepRed (10 nM), then visualized by confocal microscopy. Images were captured using a 100× oil‐immersion objective on a Nikon AIR confocal microscope. Mitochondrial ROS levels were measured by fluorescence intensity of MitoSOX Red. GFP‐expressing human BSM cells were used as a control group. Scale bars = 50 μm. (B) Quantifications of MitoSOX fluorescent intensity. Live cell confocal images of mitochondria were processed using Python‐based pipelines, as described in the Materials and Methods section. (C) ROS levels in isolated mitochondria were assessed by measuring H 2 O 2 production using the Amplex Red Hydrogen Peroxide assay in the presence of superoxide dismutase. (D) OCR was measured using a Seahorse Bioscience extracellular flux analyzer. Basal and maximal respiration were determined. OCR values were normalized to the total number of cells. (E) Quantifications of total cellular ATP levels by a bioluminescence assay. ATP levels were measured based on firefly luciferin‐luciferase luminance intensity. Data are presented as mean ± SD, and n = 25 cells in each group in B, n = 3 in C and D, and n = 4 in E. ** p < 0.01 and *** p < 0.001.

    Journal: The FASEB Journal

    Article Title: Increased Expression of Desmin and Vimentin Impairs Mitochondrial Function and Reduces Contractility via G0S2 in Bladder Smooth Muscle

    doi: 10.1096/fj.202502912R

    Figure Lengend Snippet: Increased expression of desmin and vimentin enhances mitochondrial ROS and reduces oxygen consumption rate and ATP levels. Human BSM cells were transduced with an adenovirus encoding desmin and vimentin, and GFP for 36 h. Human BSM cells overexpressing desmin and vimentin were processed for ROS, OCR, and ATP measurements. GFP‐expressing cells served as a control group. (A) Mitochondrial ROS levels in live cells were assessed by MitoSOX fluorescence. Human BSM cells overexpressing desmin and vimentin were stained with MitoSOX (5 μM) and MitoTracker DeepRed (10 nM), then visualized by confocal microscopy. Images were captured using a 100× oil‐immersion objective on a Nikon AIR confocal microscope. Mitochondrial ROS levels were measured by fluorescence intensity of MitoSOX Red. GFP‐expressing human BSM cells were used as a control group. Scale bars = 50 μm. (B) Quantifications of MitoSOX fluorescent intensity. Live cell confocal images of mitochondria were processed using Python‐based pipelines, as described in the Materials and Methods section. (C) ROS levels in isolated mitochondria were assessed by measuring H 2 O 2 production using the Amplex Red Hydrogen Peroxide assay in the presence of superoxide dismutase. (D) OCR was measured using a Seahorse Bioscience extracellular flux analyzer. Basal and maximal respiration were determined. OCR values were normalized to the total number of cells. (E) Quantifications of total cellular ATP levels by a bioluminescence assay. ATP levels were measured based on firefly luciferin‐luciferase luminance intensity. Data are presented as mean ± SD, and n = 25 cells in each group in B, n = 3 in C and D, and n = 4 in E. ** p < 0.01 and *** p < 0.001.

    Article Snippet: Mitochondria were isolated from human BSM cells and murine BSM tissue using the Mitochondria Isolation Kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer's instructions.

    Techniques: Expressing, Transduction, Control, Fluorescence, Staining, Confocal, Microscopy, Isolation, Amplex Red Hydrogen Peroxide Assay, ATP Bioluminescent Assay, Luciferase

    Overexpression of MnSOD did not rescue the decreased contractile response and reduced ATP levels in human BSM cells with elevated levels of desmin and vimentin. Human BSM cells were transfected with MnSOD cDNA and transduced with adenoviruses encoding desmin and vimentin. (A) Gel contraction : For gel contraction studies, cells were seeded in a collagen gel as described in the Materials and Methods section, and gel contraction was measured in response to 120 mM KCl stimulation. The gels were subsequently imaged. The size of collagen gel in each image was quantified using Fiji (ImageJ/NIH) software, and the percentage change was calculated. (B) Measurement of mitochondrial ROS levels : ROS levels in cells were measured using isolated mitochondria with the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit. (C) Quantifications of total cellular ATP levels : ATP levels were measured based on firefly luciferin‐luciferase luminescence intensity. (D, E) Effect of antimycin on desmin and vimentin expression . Human BSM cells were treated with the indicated concentrations of antimycin A for 36 h. The expression levels of desmin and vimentin proteins were analyzed by immunoblot. GAPDH served as a loading control. Representative immunoblots and their corresponding quantification are shown in D, E panels. Control cells received an equivalent volume of DMSO to vehicle treatment, and the final DMSO concentration was maintained at ≤ 0.1%. (F) Measurement of mitochondrial ROS : ROS levels in control and antimycin A‐treated human BSM cells were measured using isolated mitochondria with the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit. Data are presented as mean ± SD, and n = 3. The ns indicates non‐significant. * p < 0.05 and ** p < 0.01.

    Journal: The FASEB Journal

    Article Title: Increased Expression of Desmin and Vimentin Impairs Mitochondrial Function and Reduces Contractility via G0S2 in Bladder Smooth Muscle

    doi: 10.1096/fj.202502912R

    Figure Lengend Snippet: Overexpression of MnSOD did not rescue the decreased contractile response and reduced ATP levels in human BSM cells with elevated levels of desmin and vimentin. Human BSM cells were transfected with MnSOD cDNA and transduced with adenoviruses encoding desmin and vimentin. (A) Gel contraction : For gel contraction studies, cells were seeded in a collagen gel as described in the Materials and Methods section, and gel contraction was measured in response to 120 mM KCl stimulation. The gels were subsequently imaged. The size of collagen gel in each image was quantified using Fiji (ImageJ/NIH) software, and the percentage change was calculated. (B) Measurement of mitochondrial ROS levels : ROS levels in cells were measured using isolated mitochondria with the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit. (C) Quantifications of total cellular ATP levels : ATP levels were measured based on firefly luciferin‐luciferase luminescence intensity. (D, E) Effect of antimycin on desmin and vimentin expression . Human BSM cells were treated with the indicated concentrations of antimycin A for 36 h. The expression levels of desmin and vimentin proteins were analyzed by immunoblot. GAPDH served as a loading control. Representative immunoblots and their corresponding quantification are shown in D, E panels. Control cells received an equivalent volume of DMSO to vehicle treatment, and the final DMSO concentration was maintained at ≤ 0.1%. (F) Measurement of mitochondrial ROS : ROS levels in control and antimycin A‐treated human BSM cells were measured using isolated mitochondria with the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit. Data are presented as mean ± SD, and n = 3. The ns indicates non‐significant. * p < 0.05 and ** p < 0.01.

    Article Snippet: Mitochondria were isolated from human BSM cells and murine BSM tissue using the Mitochondria Isolation Kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer's instructions.

    Techniques: Over Expression, Transfection, Transduction, Software, Isolation, Luciferase, Expressing, Western Blot, Control, Concentration Assay

    Increased expression of desmin and vimentin induces mitochondrial and contractile dysfunction via G0S2 in human BSM cells. (A) Microarray analysis : Gene expression was analyzed using microarray‐based transcriptomics. A heatmap generated by hierarchical clustering illustrates trends in gene expression in human BSM cells overexpressing desmin or vimentin proteins compared with GFP‐expressing control cells. Each row represents a single mRNA probe, and the accompanying dendrogram shows the hierarchical clustering tree of the gene expression data. The colored bar at the bottom represents changes in gene expression on a linear scale, with blue indicating down‐regulation and red indicating up‐regulation. (B) G0S2 interaction with VDAC : Mitochondrial proteins from GFP‐, desmin‐, and vimentin‐overexpressing human BSM cells were subjected to co‐immunoprecipitation. Mitochondrial proteins were immunoprecipitated using an anti‐G0S2 antibody. The resulting immunoprecipitate was separated by SDS‐PAGE and probed with anti‐G0S2 and anti‐VDAC antibodies. (C) The protein expression levels of CAV1 were analyzed by immunoblotting. Representative immunoblot and its corresponding quantification are shown in panel C. (D) The levels of IL‐6 and IL‐1β were analyzed by ELISA. (E) The expression levels of VDAC protein across passages (P3–P5) were analyzed by immunoblotting. Cytochrome c oxidase IV (COXIV) served as a loading control. (F–I) Human BSM cells were transduced with adenovirus encoding GFP, desmin (Des), and vimentin (Vim) in the presence of scrambled (Scr) shRNA or G0S2 shRNA. (F, G) ROS levels in cells were measured using isolated mitochondria with the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit. (H) Quantification of total cellular ATP levels . ATP levels were measured based on firefly luciferin‐luciferase luminescence intensity. (I) BSM gel contraction : For gel contraction studies, cells were seeded in a collagen gel as described in Materials and Methods, and gel contraction was measured in response to 120 mM KCl stimulation. The gels were subsequently imaged. The size of the collagen gel in each image was quantified using Fiji (ImageJ/NIH) software, and the percentage change was calculated. Data are presented as mean ± SD, with n = 3 in A, n = 3 in C, F, G, and I, and n = 2 in D, n = 4 in H. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

    Journal: The FASEB Journal

    Article Title: Increased Expression of Desmin and Vimentin Impairs Mitochondrial Function and Reduces Contractility via G0S2 in Bladder Smooth Muscle

    doi: 10.1096/fj.202502912R

    Figure Lengend Snippet: Increased expression of desmin and vimentin induces mitochondrial and contractile dysfunction via G0S2 in human BSM cells. (A) Microarray analysis : Gene expression was analyzed using microarray‐based transcriptomics. A heatmap generated by hierarchical clustering illustrates trends in gene expression in human BSM cells overexpressing desmin or vimentin proteins compared with GFP‐expressing control cells. Each row represents a single mRNA probe, and the accompanying dendrogram shows the hierarchical clustering tree of the gene expression data. The colored bar at the bottom represents changes in gene expression on a linear scale, with blue indicating down‐regulation and red indicating up‐regulation. (B) G0S2 interaction with VDAC : Mitochondrial proteins from GFP‐, desmin‐, and vimentin‐overexpressing human BSM cells were subjected to co‐immunoprecipitation. Mitochondrial proteins were immunoprecipitated using an anti‐G0S2 antibody. The resulting immunoprecipitate was separated by SDS‐PAGE and probed with anti‐G0S2 and anti‐VDAC antibodies. (C) The protein expression levels of CAV1 were analyzed by immunoblotting. Representative immunoblot and its corresponding quantification are shown in panel C. (D) The levels of IL‐6 and IL‐1β were analyzed by ELISA. (E) The expression levels of VDAC protein across passages (P3–P5) were analyzed by immunoblotting. Cytochrome c oxidase IV (COXIV) served as a loading control. (F–I) Human BSM cells were transduced with adenovirus encoding GFP, desmin (Des), and vimentin (Vim) in the presence of scrambled (Scr) shRNA or G0S2 shRNA. (F, G) ROS levels in cells were measured using isolated mitochondria with the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit. (H) Quantification of total cellular ATP levels . ATP levels were measured based on firefly luciferin‐luciferase luminescence intensity. (I) BSM gel contraction : For gel contraction studies, cells were seeded in a collagen gel as described in Materials and Methods, and gel contraction was measured in response to 120 mM KCl stimulation. The gels were subsequently imaged. The size of the collagen gel in each image was quantified using Fiji (ImageJ/NIH) software, and the percentage change was calculated. Data are presented as mean ± SD, with n = 3 in A, n = 3 in C, F, G, and I, and n = 2 in D, n = 4 in H. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

    Article Snippet: Mitochondria were isolated from human BSM cells and murine BSM tissue using the Mitochondria Isolation Kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer's instructions.

    Techniques: Expressing, Microarray, Analysis, Gene Expression, Transcriptomics, Generated, Control, Co-Immunoprecipitation Assay, Immunoprecipitation, SDS Page, Western Blot, Enzyme-linked Immunosorbent Assay, Transduction, shRNA, Isolation, Luciferase, Software

    Increased expression of desmin and vimentin induces contractile and mitochondrial dysfunction via G0S2 in murine BSM tissue. BSM strips from WT and G0S2 KO mice were transduced with an adenovirus encoding GFP, desmin, and vimentin for 36 h. BSM contraction : (A–D) The contractile responses of murine BSM strips overexpressing desmin and vimentin in response to KCl (120 mM) and carbachol (10 −9 to 10 −4 M) stimulation were determined. (E) Quantifications of ROS levels : ROS levels in murine BSM strips were measured using isolated mitochondria with the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit. (F) Quantifications of total cellular ATP levels . ATP levels were measured based on firefly luciferin‐luciferase luminance intensity. Data are presented as mean ± SD and n = 5 in A–F.

    Journal: The FASEB Journal

    Article Title: Increased Expression of Desmin and Vimentin Impairs Mitochondrial Function and Reduces Contractility via G0S2 in Bladder Smooth Muscle

    doi: 10.1096/fj.202502912R

    Figure Lengend Snippet: Increased expression of desmin and vimentin induces contractile and mitochondrial dysfunction via G0S2 in murine BSM tissue. BSM strips from WT and G0S2 KO mice were transduced with an adenovirus encoding GFP, desmin, and vimentin for 36 h. BSM contraction : (A–D) The contractile responses of murine BSM strips overexpressing desmin and vimentin in response to KCl (120 mM) and carbachol (10 −9 to 10 −4 M) stimulation were determined. (E) Quantifications of ROS levels : ROS levels in murine BSM strips were measured using isolated mitochondria with the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit. (F) Quantifications of total cellular ATP levels . ATP levels were measured based on firefly luciferin‐luciferase luminance intensity. Data are presented as mean ± SD and n = 5 in A–F.

    Article Snippet: Mitochondria were isolated from human BSM cells and murine BSM tissue using the Mitochondria Isolation Kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer's instructions.

    Techniques: Expressing, Tissue, Transduction, Isolation, Luciferase