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cdna synthesis kit iscripttm advanced  (Promega)

 
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    Promega cdna synthesis kit iscripttm advanced
    Cdna Synthesis Kit Iscripttm Advanced, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscripttm+cdna+kit/iscript+cdna+synthesis+kit/pmc08381024-54-6-19
    Average 90 stars, based on 1 article reviews
    cdna synthesis kit iscripttm advanced - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Optimization of seeding density of OP9 cells to improve hematopoietic differentiation efficiency
    Article Snippet: .. Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, USA). cDNA was synthesized using 1 μg of RNA from each sample with iScriptTM cDNA Synthesis Kit (Promega, USA). .. RT-PCR cycling conditions were as follows: initial denaturation at 95 °C for 10 min followed by 40 denaturation cycles at 95 °C for 15 s, primer annealing at 60 °C for 60 s, primer extension phase at 72 °C 15 s and final extension step at 72 °C for 7 min (Agilent Stratagene Mx3000P, Agilent Technologies, USA).

    Synthesized:

    Article Title: Optimization of seeding density of OP9 cells to improve hematopoietic differentiation efficiency
    Article Snippet: .. Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, USA). cDNA was synthesized using 1 μg of RNA from each sample with iScriptTM cDNA Synthesis Kit (Promega, USA). .. RT-PCR cycling conditions were as follows: initial denaturation at 95 °C for 10 min followed by 40 denaturation cycles at 95 °C for 15 s, primer annealing at 60 °C for 60 s, primer extension phase at 72 °C 15 s and final extension step at 72 °C for 7 min (Agilent Stratagene Mx3000P, Agilent Technologies, USA).

    cDNA Synthesis:

    Article Title: Optimization of seeding density of OP9 cells to improve hematopoietic differentiation efficiency
    Article Snippet: .. Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, USA). cDNA was synthesized using 1 μg of RNA from each sample with iScriptTM cDNA Synthesis Kit (Promega, USA). .. RT-PCR cycling conditions were as follows: initial denaturation at 95 °C for 10 min followed by 40 denaturation cycles at 95 °C for 15 s, primer annealing at 60 °C for 60 s, primer extension phase at 72 °C 15 s and final extension step at 72 °C for 7 min (Agilent Stratagene Mx3000P, Agilent Technologies, USA).

    Article Title: Two Triterpenoids, ARM-2 and RA-5, From Protorhus longifolia Exhibit the Potential to Modulate Lipolysis and Lipogenesis in Cultured 3T3-L1 Adipocytes
    Article Snippet: The RNA was extracted with the Promega RNA extraction kit. .. The RNA was then converted into cDNA using an iScript cDNA Synthesis Kit from Promega following the manufacturer's protocols. qRT–PCR analysis was performed using Bio-Rad CFX Maestro software. ..

    Article Title: The immune response modulated by inoculation of commensal bacteria at birth impacts the gut microbiota and prevents Salmonella colonization.
    Article Snippet: RNA purity and concentration were measured with a Nanodrop (Thermoscientific Nanodrop, IllkirchGraffenstaden, France) and their integrity was assessed by 1% agarose gel electrophoresis. .. 200 ng of RNA from each sample were reverse-transcribed using iScriptTM cDNA Synthesis kit (Promega, Charbonnières-les-Bains, France) as described by the manufacturer and the cDNAs obtained were stored at −20°C and diluted just before use. .. The primer sequences used in this study have been partly obtained by Fluidigm D3TM (Fluidigm France, Les Ulis, France) assay design service (Supplementary Table SIII).

    Article Title: Triterpenoids from Protorhus longifolia Exhibit Hypocholesterolemic Potential via Regulation of Cholesterol Biosynthesis and Stimulation of Low-Density Lipoprotein Uptake in HepG2 Cells
    Article Snippet: .. Total RNA from the cells was extracted using an RNA extraction kit (Promega) and converted to cDNA using the iScript cDNA synthesis kit (Promega) as described in the manufacturer’s protocols. ..

    Article Title: Probiotic lactic acid bacteria alleviate pediatric IBD and remodel gut microbiota by modulating macrophage polarization and suppressing epithelial apoptosis
    Article Snippet: .. The kit of iScriptTM cDNA synthesis was bought from Promega (WI, USA). .. Radio immunoprecipitation assay lysis buffer was obtained from Beyotime Biotechnology Co. Ltd (Nantong, China).

    Article Title: Phylogenetic and Expression Analysis of Fos Transcription Factors in Zebrafish.
    Article Snippet: .. Total RNA was isolated from zebrafish embryos using TRIsure (Bioline) according to the manufacturer’s instructions and cDNA synthesis was performed using an iScript cDNA synthesis kit (Promega). .. RT-PCR was performed to amplify cDNA by using the Go Taq Green Master Mix (Promega, Madison, USA) using the following primers: fosaa (5′-GGAAGAGCAAGAGCGAGCA, 5′-CTTGTAGAGCGTCTCCCAGTC), fosab (5′-TGACA GGATGATGTTTACCAGC, 5′-CGGCTCCAGGTCAGTGTTAG), fosb (5′-ACAGTAGGTGTT TCGGCTTCTC, 5′-GGTGGTGGGATGAGTAGGC), fosl1a (5′-AAACGCCAGCAGAGAGTGTC, 5′-GGTAAATGGAGTCAGGGATGG), fosl1b (5′-ACACCAACCAGCCACGAAAC, 5′-CGG GAATCATAATACGACTCTC), and fosl2 (5′-TGTCGGAACCGCAGAAGAG, 5′-ATCTCTC CTCTGGCTTTACCTTC).

    other:

    Article Title: TRPV6 is a prognostic marker in early-stage cervical squamous cell carcinoma.
    Article Snippet: Transient receptor potential vanilloid 6 (TRPV6) has been shown to promote caner proliferation in several solid tumors, leading to unfavorable clinical outcomes.. Our study aimed to elucidate the clinical significance of TRPV6 in patients with early-stage cervical squamous cell carcinoma (CSCC).. The mRNA expression of TRPV6 was measured in 12 paired early-stage CSCC specimens and six cervical carcinoma cell lines using quantitative real-time PCR (qRT-PCR).

    Article Title: Adeno-associated virus-delivered alpha synuclein inhibits bladder cancer growth via the p53/p21 signaling pathway.
    Article Snippet: Alpha-synuclein (α-syn), encoded by the SNCA gene, is a major participant in the pathophysiology of Parkinson’s disease (PD).. Its functions have been reported to be related to apoptosis induction, the elevation of oxidative stress, mitochondrial homeostasis, cell-cycle aberrations, and DNA-related interactions.. Evidence obtained in recent studies suggests a possible link between α-syn and cancer development.

    Maestro Software:

    Article Title: Two Triterpenoids, ARM-2 and RA-5, From Protorhus longifolia Exhibit the Potential to Modulate Lipolysis and Lipogenesis in Cultured 3T3-L1 Adipocytes
    Article Snippet: The RNA was extracted with the Promega RNA extraction kit. .. The RNA was then converted into cDNA using an iScript cDNA Synthesis Kit from Promega following the manufacturer's protocols. qRT–PCR analysis was performed using Bio-Rad CFX Maestro software. ..

    Reverse Transcription:

    Article Title: The immune response modulated by inoculation of commensal bacteria at birth impacts the gut microbiota and prevents Salmonella colonization.
    Article Snippet: RNA purity and concentration were measured with a Nanodrop (Thermoscientific Nanodrop, IllkirchGraffenstaden, France) and their integrity was assessed by 1% agarose gel electrophoresis. .. 200 ng of RNA from each sample were reverse-transcribed using iScriptTM cDNA Synthesis kit (Promega, Charbonnières-les-Bains, France) as described by the manufacturer and the cDNAs obtained were stored at −20°C and diluted just before use. .. The primer sequences used in this study have been partly obtained by Fluidigm D3TM (Fluidigm France, Les Ulis, France) assay design service (Supplementary Table SIII).

    RNA Extraction:

    Article Title: Triterpenoids from Protorhus longifolia Exhibit Hypocholesterolemic Potential via Regulation of Cholesterol Biosynthesis and Stimulation of Low-Density Lipoprotein Uptake in HepG2 Cells
    Article Snippet: .. Total RNA from the cells was extracted using an RNA extraction kit (Promega) and converted to cDNA using the iScript cDNA synthesis kit (Promega) as described in the manufacturer’s protocols. ..

    Isolation:

    Article Title: Phylogenetic and Expression Analysis of Fos Transcription Factors in Zebrafish.
    Article Snippet: .. Total RNA was isolated from zebrafish embryos using TRIsure (Bioline) according to the manufacturer’s instructions and cDNA synthesis was performed using an iScript cDNA synthesis kit (Promega). .. RT-PCR was performed to amplify cDNA by using the Go Taq Green Master Mix (Promega, Madison, USA) using the following primers: fosaa (5′-GGAAGAGCAAGAGCGAGCA, 5′-CTTGTAGAGCGTCTCCCAGTC), fosab (5′-TGACA GGATGATGTTTACCAGC, 5′-CGGCTCCAGGTCAGTGTTAG), fosb (5′-ACAGTAGGTGTT TCGGCTTCTC, 5′-GGTGGTGGGATGAGTAGGC), fosl1a (5′-AAACGCCAGCAGAGAGTGTC, 5′-GGTAAATGGAGTCAGGGATGG), fosl1b (5′-ACACCAACCAGCCACGAAAC, 5′-CGG GAATCATAATACGACTCTC), and fosl2 (5′-TGTCGGAACCGCAGAAGAG, 5′-ATCTCTC CTCTGGCTTTACCTTC).



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