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dedicated kit  (Bio-Rad)


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    Structured Review

    Bio-Rad dedicated kit
    Dedicated Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript/iScript+Explore+One-Step+RT+and+PreAmp+Kit/pmc08945364-110-6-15
    Average 93 stars, based on 90 article reviews
    dedicated kit - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Monitoring Sexually Transmitted Infection-Associated Pathogens in Municipal Wastewater in Japan
    Article Snippet: Briefly, 13.5 μL of nucleic acid extract was mixed with 16.5 μL of a preamp mixture containing 15.0 μL of SsoAdvanced Preamp Supermix (Bio-Rad Laboratories, Hercules, CA, USA), 0.3 μL of primer mix (10 μM each of forward and reverse primers; Supporting Information, Table S3), and 1.2 μL of nuclease-free water to obtain a preamp product with a total volume of 30 μL (Supporting Information, Table S4). .. For PMMoV, one-step reverse transcription (RT) preamp was performed using an iScript Explore One-Step RT and PreAmp Kit (Bio-Rad). ..

    Article Title: Differential lncRNA Expression in Undifferentiated and Differentiated LUHMES Cells Following Co-Exposure to Silver Nanoparticles and Nanoplastic.
    Article Snippet: RNA concentration was measured using a Quantus Fluorometer (Promega, Madison, WI, USA) and the QuantiFluor RNA System (Promega, Madison, WI, USA). .. One microgram of total RNA was reverse-transcribed and preamplified using the iScript Explore One-Step RT and PreAmp Kit (Bio-Rad, Hercules, CA, USA) following the manufacturer’s instructions. .. The cDNA was diluted ten times with deionized, RNAse-free H2O and used for lncRNA expression profiling using the Inflammation lncRNA H96 PrimePCR PCR Array (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.

    Article Title: A cardiac extracellular matrix-based bilayer vascular graft with controlled microstructures for the reconstruction of small-diameter blood vessels.
    Article Snippet: Despite recent progress, challenges with small-diameter vascular grafts, including mechanical strength, intimal hyperplasia, thrombosis, and poor endothelialization, remain unresolved.. The present study reports a novel bilayer vascular graft designed to mimic the anatomical features of small-diameter blood vessels.. The electrospun graft consists of a dense micro/nanofibrous inner layer of cardiac extracellular matrix (cECM), polycaprolactone (PCL) loaded with heparin (P-cECM-H), and a super porous and micro-fibrous PCL outer layer.

    Article Title: Differential lncRNA Expression in Undifferentiated and Differentiated LUHMES Cells Following Co-Exposure to Silver Nanoparticles and Nanoplastic
    Article Snippet: RNA concentration was measured using a Quantus Fluorometer (Promega, Madison, WI, USA) and the QuantiFluor RNA System (Promega, Madison, WI, USA). .. One microgram of total RNA was reverse-transcribed and preamplified using the iScript Explore One-Step RT and PreAmp Kit (Bio-Rad, Hercules, CA, USA) following the manufacturer’s instructions. .. The cDNA was diluted ten times with deionized, RNAse-free H 2 O and used for lncRNA expression profiling using the Inflammation lncRNA H96 PrimePCR PCR Array (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: Simultaneous detection of protozoa, bacteria, and viruses from environmental water through membrane-adsorption followed by direct nucleic acid extraction
    Article Snippet: A 7.5 μL of the extract was subjected to reverse transcription (RT) using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific,) to obtain 15 μL of (c)DNA, following the manufacturer’s protocol. .. One-step RT-preamplification was performed to prepare a template for real-time PCR using iScript TM Explore One-Step RT and PreAmp Kit (Bio-Rad) according to a previous study ( ). ..

    cDNA Synthesis:

    Article Title: The E3 ubiquitin ligase DTX3L and the deubiquitinase USP28 fine-tune DNA repair through mutual regulation of their protein levels
    Article Snippet: RNeasy Mini Kit , Qiagen , #73404. .. iScript cDNA Synthesis Kit , Bio-Rad , #12004856. .. iTaq SYBR Green Supermix , Bio-Rad , #1725120.



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