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SuperArray Bioscience Corporation rt2 first strand kit
Rt2 First Strand Kit, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript+reverse+transcriptase+kit/rt2+first+strand+kit/pmc03288342-220-14-18
Average 90 stars, based on 1 article reviews
rt2 first strand kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Reverse Transcription Polymerase Chain Reaction:

Article Title: Forkhead Box q1 promotes invasion and metastasis in colorectal cancer by activating the epidermal growth factor receptor pathway
Article Snippet: .. The RNA samples were kept on ice for another 5 min and then converted into cDNA with the RT PCR Array First Strand Kit (SuperArray) according to the manufacturer’s protocol. cDNA (20 ng) was combined with RT SYBR Green/Fluorescein PCR master mix (SuperArray), and equal amounts of this mixture (25 μL) were added to each well of the RT Profiler PCR plate containing the predispensed gene-specific primer sets. ..

Polymerase Chain Reaction:

Article Title: Forkhead Box q1 promotes invasion and metastasis in colorectal cancer by activating the epidermal growth factor receptor pathway
Article Snippet: .. The RNA samples were kept on ice for another 5 min and then converted into cDNA with the RT PCR Array First Strand Kit (SuperArray) according to the manufacturer’s protocol. cDNA (20 ng) was combined with RT SYBR Green/Fluorescein PCR master mix (SuperArray), and equal amounts of this mixture (25 μL) were added to each well of the RT Profiler PCR plate containing the predispensed gene-specific primer sets. ..

Article Title: Forkhead Box q1 promotes invasion and metastasis in colorectal cancer by activating the epidermal growth factor receptor pathway.
Article Snippet: .. The RNA samples were kept on ice for another 5 min and then converted into cDNA with the RT2 PCR Array First Strand Kit (SuperArray) according to the manufacturer’s protocol. cDNA (20 ng) was combined with RT2 SYBR Green/ Fluorescein PCR master mix (SuperArray), and equal amounts of this mixture (25 μL) were added to each well of the RT2 Profiler PCR plate containing the predispensed gene-specific primer sets. ..

Article Title: Absence of Heme Oxygenase-1 Affects Trophoblastic Spheroid Implantation and Provokes Dysregulation of Stress and Angiogenesis Gene Expression in the Uterus
Article Snippet: .. Thereafter, the RNA concentration of the pools was measured, and 1 μg of total RNA from each pool was reverse transcribed using the RT 2 PCR Array First Strand Kit (C-02, SuperArray Bioscience, Frederick, MD, USA). .. The generated cDNA was diluted with an appropriate volume of instrument-specific 2x SuperArray RT2 Real-TimeTM SYBR Green PCR Master Mix (PA-012, SuperArray Bioscience, Frederick, MD, USA) and ultra-pure water, and 25 μL of this reaction mix was added to each well of the PCR array.

RNA Extraction:

Article Title: Vaccine and methods for detecting and preventing filariasis
Article Snippet: After 72 hours, cells were centrifuged (1000 rpm for 5 minutes) and total RNA was extracted from the cell pellets using TRIZOL (phenol, guanidinium and thiocyanate) reagent (Invitrogen) as per description of the manufacturer. .. Followed by RNA extraction, first-strand cDNA was synthesized by RT2 First Strand Kit (SuperArray Bioscience Corporation, Frederick, Md.). ..

Article Title: Vaccine and methods for detecting and preventing filariasis
Article Snippet: After 72 hours, cells were centrifuged (1000 rpm for 5 minutes) and total RNA was extracted from the cell pellets using TRIZOL (phenol, guanidinium and thiocyanate) reagent (Invitrogen) as per description of the manufacturer. .. Followed by RNA extraction, first-strand cDNA was synthesized by RT2 First Strand Kit (SuperArray Bioscience Corporation, Frederick, MD). ..

Synthesized:

Article Title: Vaccine and methods for detecting and preventing filariasis
Article Snippet: After 72 hours, cells were centrifuged (1000 rpm for 5 minutes) and total RNA was extracted from the cell pellets using TRIZOL (phenol, guanidinium and thiocyanate) reagent (Invitrogen) as per description of the manufacturer. .. Followed by RNA extraction, first-strand cDNA was synthesized by RT2 First Strand Kit (SuperArray Bioscience Corporation, Frederick, Md.). ..

Article Title: Inhibition of acid sphingomyelinase by imipramine abolishes the synergy between metabolic syndrome and periodontitis on alveolar bone loss.
Article Snippet: .. First- strand cDNA was synthesized from RNA using RT2 First Strand Kit (SuperArray Bioscience Corp.). .. Mouse toll- like receptor (TLR) pathway- focused PCR Arrays (SuperArray Bioscience Corp.) were performed using 2× SuperArray RT2 qPCR master mix and the first- strand cDNA by following the instructions from the manufacturer.

Article Title: GPR40 deficiency worsens metabolic syndrome-associated periodontitis in mice.
Article Snippet: © 2023 John Wiley & Sons Ltd.. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.. 1Division of Endocrinology, Diabetes and Metabolic Diseases, Department of Medicine, College of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA 2Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, New York, USA 3Department of Head & Neck/Plastic & Reconstructive Surgery, Roswell Park Comprehensive Cancer Center, Buffalo, New York, USA 4National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland, USA 5Integrative Physiology and Neuroscience, Washington State University, Pullman, Washington, USA 6Ralph H. Johnson Veterans Affairs Medical Center, Charleston, South Carolina, USA

Article Title: Vaccine and methods for detecting and preventing filariasis
Article Snippet: After 72 hours, cells were centrifuged (1000 rpm for 5 minutes) and total RNA was extracted from the cell pellets using TRIZOL (phenol, guanidinium and thiocyanate) reagent (Invitrogen) as per description of the manufacturer. .. Followed by RNA extraction, first-strand cDNA was synthesized by RT2 First Strand Kit (SuperArray Bioscience Corporation, Frederick, MD). ..

Concentration Assay:

Article Title: Absence of Heme Oxygenase-1 Affects Trophoblastic Spheroid Implantation and Provokes Dysregulation of Stress and Angiogenesis Gene Expression in the Uterus
Article Snippet: .. Thereafter, the RNA concentration of the pools was measured, and 1 μg of total RNA from each pool was reverse transcribed using the RT 2 PCR Array First Strand Kit (C-02, SuperArray Bioscience, Frederick, MD, USA). .. The generated cDNA was diluted with an appropriate volume of instrument-specific 2x SuperArray RT2 Real-TimeTM SYBR Green PCR Master Mix (PA-012, SuperArray Bioscience, Frederick, MD, USA) and ultra-pure water, and 25 μL of this reaction mix was added to each well of the PCR array.

Reverse Transcription:

Article Title: Absence of Heme Oxygenase-1 Affects Trophoblastic Spheroid Implantation and Provokes Dysregulation of Stress and Angiogenesis Gene Expression in the Uterus
Article Snippet: .. Thereafter, the RNA concentration of the pools was measured, and 1 μg of total RNA from each pool was reverse transcribed using the RT 2 PCR Array First Strand Kit (C-02, SuperArray Bioscience, Frederick, MD, USA). .. The generated cDNA was diluted with an appropriate volume of instrument-specific 2x SuperArray RT2 Real-TimeTM SYBR Green PCR Master Mix (PA-012, SuperArray Bioscience, Frederick, MD, USA) and ultra-pure water, and 25 μL of this reaction mix was added to each well of the PCR array.



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