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annexin v fitc pi apoptosis detection kit  (Beyotime)


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    Structured Review

    Beyotime annexin v fitc pi apoptosis detection kit
    Effect of MTX on the biological function of HUT-78 cells. (A) Chemical formula of MTX. (B) Effect of different concentrations of MTX on cell survival. (C) Flow cytometric analysis of (D) the effects of different concentrations of MTX on cells effects of <t>apoptosis.</t> Data are presented as the mean ± SD, n=3, and were analysed using a one-way ANOVA with Tukey's test. *P<0.05, **P<0.01, ***P<0.005 vs. NC. MTX, methotrexate; NC, negative control.
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 3631 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+kit/pmc07551555-57-5-11?v=Beyotime
    Average 99 stars, based on 3631 article reviews
    annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2026-07
    99/100 stars

    Images

    1) Product Images from "HSH2D contributes to methotrexate resistance in human T-cell acute lymphoblastic leukaemia"

    Article Title: HSH2D contributes to methotrexate resistance in human T-cell acute lymphoblastic leukaemia

    Journal: Oncology Reports

    doi: 10.3892/or.2020.7772

    Effect of MTX on the biological function of HUT-78 cells. (A) Chemical formula of MTX. (B) Effect of different concentrations of MTX on cell survival. (C) Flow cytometric analysis of (D) the effects of different concentrations of MTX on cells effects of apoptosis. Data are presented as the mean ± SD, n=3, and were analysed using a one-way ANOVA with Tukey's test. *P<0.05, **P<0.01, ***P<0.005 vs. NC. MTX, methotrexate; NC, negative control.
    Figure Legend Snippet: Effect of MTX on the biological function of HUT-78 cells. (A) Chemical formula of MTX. (B) Effect of different concentrations of MTX on cell survival. (C) Flow cytometric analysis of (D) the effects of different concentrations of MTX on cells effects of apoptosis. Data are presented as the mean ± SD, n=3, and were analysed using a one-way ANOVA with Tukey's test. *P<0.05, **P<0.01, ***P<0.005 vs. NC. MTX, methotrexate; NC, negative control.

    Techniques Used: Negative Control

    HSH2D is essential for MTX resistance of HuT-78 cells. (A) Silencing efficacy was evaluated using reverse transcription-quantitative PCR after transfection of two siRNAs and a NC of HSH2D. (B) Knockdown of HSH2D promoted apoptosis induced by MTX treatment in both MTX-resistant cell lines. (C) IC 50 value of MTX was detected for both sensitive and resistant cells using a Cell Counting Kit-8 assay. (D) EdU results of the cell proliferation of drug resistant strains with HSH2D knockdown. (E) Flow cytometry was used to detected (F) the cell cycle in drug resistant strains with HSH2D knockdown. Data are presented as the mean ± SD, n=3, and were analysed using a two-tailed Student's t-test or one-way ANOVA. *P<0.05 and **P<0.01 vs. siNC, siRNA negative control; MTX, methotrexate; HSH2D, haematopoietic SH2 domain containing.
    Figure Legend Snippet: HSH2D is essential for MTX resistance of HuT-78 cells. (A) Silencing efficacy was evaluated using reverse transcription-quantitative PCR after transfection of two siRNAs and a NC of HSH2D. (B) Knockdown of HSH2D promoted apoptosis induced by MTX treatment in both MTX-resistant cell lines. (C) IC 50 value of MTX was detected for both sensitive and resistant cells using a Cell Counting Kit-8 assay. (D) EdU results of the cell proliferation of drug resistant strains with HSH2D knockdown. (E) Flow cytometry was used to detected (F) the cell cycle in drug resistant strains with HSH2D knockdown. Data are presented as the mean ± SD, n=3, and were analysed using a two-tailed Student's t-test or one-way ANOVA. *P<0.05 and **P<0.01 vs. siNC, siRNA negative control; MTX, methotrexate; HSH2D, haematopoietic SH2 domain containing.

    Techniques Used: Reverse Transcription, Real-time Polymerase Chain Reaction, Transfection, Knockdown, Cell Counting, Flow Cytometry, Two Tailed Test, Negative Control



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