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iscript advanced reverse transcriptase kit  (Bio-Rad)


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    Structured Review

    Bio-Rad iscript advanced reverse transcriptase kit
    Iscript Advanced Reverse Transcriptase Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 615 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+advanced+cdna+reverse+transcriptase+kit/ISCRIPT+GDNA+CLR+CDNA+KT%2C100RX/pmc10452655-64-4-38
    Average 99 stars, based on 615 article reviews
    iscript advanced reverse transcriptase kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Exacerbated ATP transmission in the carotid body is linked to glomus cell expansion in spontaneously hypertensive rats
    Article Snippet: figure legend Exacerbated ATP signalling in the carotid body (CB) of spontaneously hypertensive rats (SHRs) arises from expansion of the chemosensory glomus cell compartment and reduced extracellular ATP metabolism, © 2026 The Author(s).. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.. DOI: 10.1113/JP288931 This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    Article Title: ELABELA Targets Mitochondria to Modulate Heart Development.
    Article Snippet: .. Reverse transcription of DNA was performed using iScript gDNA Clear cDNA Syntheis kit (BIO-RAD, 1725035) and was perfomed by quantitative T-PCR protocal using PowerUP SYBR Green Master Mix (TheroFisher, A25741)on an Applied Biosystems 7500 system. .. The elative quantification of expression was determined using the ̂ Δct ×103 method, and glyceraldehyde-3-phosphate dehydrogease (GAPDH, human) was used as an internal control. otal RNA for cells was extracted using TRIzol reagent (Invitroen, 15596018), and reverse transcription of cDNA was performed ith PrimeScript RT Master Mix (Takara, RR047A).

    Article Title: Enforced ZFP281 expression delays breast cancer initiation and can provide lifelong protection against breast cancer metastasis
    Article Snippet: Total RNA was extracted using the RNeasy Plus Micro kit (Qiagen, 74034). .. Reverse transcription was performed using the iScript gDNA clear cDNA synthesis kit (BioRad, 1725035). .. Relative expression levels of RNA were determined using PowerUP SYBR TM Green master Mix (Applied Biosystems, A25776) by a QuantStudio 3 Real-Time PCR System (Applied Biosystems, A28567).

    cDNA Synthesis:

    Article Title: Exacerbated ATP transmission in the carotid body is linked to glomus cell expansion in spontaneously hypertensive rats
    Article Snippet: figure legend Exacerbated ATP signalling in the carotid body (CB) of spontaneously hypertensive rats (SHRs) arises from expansion of the chemosensory glomus cell compartment and reduced extracellular ATP metabolism, © 2026 The Author(s).. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.. DOI: 10.1113/JP288931 This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial

    Article Title: Human iPSC-Derived Dorsal Root Ganglion Organoid Modeling of Chemotherapy-Induced Peripheral Neuropathy
    Article Snippet: .. RNA was extracted from iDRGOs using TRIzol (ThermoFisher Scientific TM , Catalog No., Waltham, MA, USA), chloroform (Sigma-Aldrich, Catalog No., St. Louis, MO, USA), and isopropanol precipitation. cDNA was synthesized from RNA using iScript gDNA cDNA Synthesis Kit (BioRAD, Catalog No. 172503, Hercules, CA, USA). .. Quantitative RT-PCR was performed using iTaq Universal SYBR Green One-Step Kit (BioRAD, Catalog No. 1725150, Hercules, CA, USA).

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    Article Title: Stage-specific CHI3L1/YKL-40 signaling controls generation of oligodendrocyte precursor cells through IL13Rα2-mediated ferroptosis
    Article Snippet: Acetylcysteine (N-Acetylcysteine, MCE: HY-B0215), Azeliragon (TTP488, MCE: HY-50682), Deferoxamine Mesylate (MCE: HY-B0988), Aspartic acid (MCE: HY-42068), FPS-ZM1 (MCE: HY-19370), 1,5-Isoquinolinediol (MCE: HY-W015422), Z-VAD-FMK (MCE: HY-16658B), Necrostatin-1 (MCE: HY-15760), Ferrostatin-1 (MCE: HY-100579), Disulfiram (Tetraethylthiuram disulfide, MCE: HY-B0240) were incubated in culture as indicated. .. mRNA was harvested with the Qiagen RNeasy Mini Kit (Qiagen #74104). cDNA was synthesized following the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035). .. SYBR green probes (obtained from Bio-Rad) and FAM probes (obtained from iDT) targeting genes of interest were applied to evaluate gene expression under the CFX96 Touch Real-Time PCR Detection System.

    Article Title: Enforced ZFP281 expression delays breast cancer initiation and can provide lifelong protection against breast cancer metastasis
    Article Snippet: Total RNA was extracted using the RNeasy Plus Micro kit (Qiagen, 74034). .. Reverse transcription was performed using the iScript gDNA clear cDNA synthesis kit (BioRad, 1725035). .. Relative expression levels of RNA were determined using PowerUP SYBR TM Green master Mix (Applied Biosystems, A25776) by a QuantStudio 3 Real-Time PCR System (Applied Biosystems, A28567).

    Article Title: Human iPSC-Derived Dorsal Root Ganglion Organoid Modeling of Chemotherapy-Induced Peripheral Neuropathy.
    Article Snippet: .. RNA was extracted from iDRGOs using TRIzol (ThermoFisher ScientificTM, Catalog No., Waltham, MA, USA), chloroform (Sigma-Aldrich, Catalog No., St. Louis, MO, USA), and isopropanol precipitation. cDNA was synthesized from RNA using iScript gDNA cDNA Synthesis Kit (BioRAD, Catalog No. 172503, Hercules, CA, USA). .. Quantitative RT-PCR was performed using iTaq Universal SYBR Green One-Step Kit (BioRAD, Catalog No. 1725150, Hercules, CA, USA).

    Article Title: Cisplatin exposure alters long-term metabolic phenotype of male, but not female, high-fat diet-fed mice
    Article Snippet: For liver samples, RNA was extracted from flash-frozen tissue using TRIzolTM reagent (Invitrogen, #15596018), as per manufacturer’s instructions. .. DNase treatment was performed prior to cDNA synthesis using the iScript gDNA Clear cDNA synthesis Kit (Bio-Rad, #1725035). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, #1725271) and run on a CFX384 (Bio-Rad). ..

    Synthesized:

    Article Title: Human iPSC-Derived Dorsal Root Ganglion Organoid Modeling of Chemotherapy-Induced Peripheral Neuropathy
    Article Snippet: .. RNA was extracted from iDRGOs using TRIzol (ThermoFisher Scientific TM , Catalog No., Waltham, MA, USA), chloroform (Sigma-Aldrich, Catalog No., St. Louis, MO, USA), and isopropanol precipitation. cDNA was synthesized from RNA using iScript gDNA cDNA Synthesis Kit (BioRAD, Catalog No. 172503, Hercules, CA, USA). .. Quantitative RT-PCR was performed using iTaq Universal SYBR Green One-Step Kit (BioRAD, Catalog No. 1725150, Hercules, CA, USA).

    Article Title: Stage-specific CHI3L1/YKL-40 signaling controls generation of oligodendrocyte precursor cells through IL13Rα2-mediated ferroptosis
    Article Snippet: Acetylcysteine (N-Acetylcysteine, MCE: HY-B0215), Azeliragon (TTP488, MCE: HY-50682), Deferoxamine Mesylate (MCE: HY-B0988), Aspartic acid (MCE: HY-42068), FPS-ZM1 (MCE: HY-19370), 1,5-Isoquinolinediol (MCE: HY-W015422), Z-VAD-FMK (MCE: HY-16658B), Necrostatin-1 (MCE: HY-15760), Ferrostatin-1 (MCE: HY-100579), Disulfiram (Tetraethylthiuram disulfide, MCE: HY-B0240) were incubated in culture as indicated. .. mRNA was harvested with the Qiagen RNeasy Mini Kit (Qiagen #74104). cDNA was synthesized following the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035). .. SYBR green probes (obtained from Bio-Rad) and FAM probes (obtained from iDT) targeting genes of interest were applied to evaluate gene expression under the CFX96 Touch Real-Time PCR Detection System.

    Article Title: Human iPSC-Derived Dorsal Root Ganglion Organoid Modeling of Chemotherapy-Induced Peripheral Neuropathy.
    Article Snippet: .. RNA was extracted from iDRGOs using TRIzol (ThermoFisher ScientificTM, Catalog No., Waltham, MA, USA), chloroform (Sigma-Aldrich, Catalog No., St. Louis, MO, USA), and isopropanol precipitation. cDNA was synthesized from RNA using iScript gDNA cDNA Synthesis Kit (BioRAD, Catalog No. 172503, Hercules, CA, USA). .. Quantitative RT-PCR was performed using iTaq Universal SYBR Green One-Step Kit (BioRAD, Catalog No. 1725150, Hercules, CA, USA).

    Quantitative RT-PCR:

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    Article Title: Cisplatin exposure alters long-term metabolic phenotype of male, but not female, high-fat diet-fed mice
    Article Snippet: For liver samples, RNA was extracted from flash-frozen tissue using TRIzolTM reagent (Invitrogen, #15596018), as per manufacturer’s instructions. .. DNase treatment was performed prior to cDNA synthesis using the iScript gDNA Clear cDNA synthesis Kit (Bio-Rad, #1725035). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, #1725271) and run on a CFX384 (Bio-Rad). ..

    SYBR Green Assay:

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    Article Title: ELABELA Targets Mitochondria to Modulate Heart Development.
    Article Snippet: .. Reverse transcription of DNA was performed using iScript gDNA Clear cDNA Syntheis kit (BIO-RAD, 1725035) and was perfomed by quantitative T-PCR protocal using PowerUP SYBR Green Master Mix (TheroFisher, A25741)on an Applied Biosystems 7500 system. .. The elative quantification of expression was determined using the ̂ Δct ×103 method, and glyceraldehyde-3-phosphate dehydrogease (GAPDH, human) was used as an internal control. otal RNA for cells was extracted using TRIzol reagent (Invitroen, 15596018), and reverse transcription of cDNA was performed ith PrimeScript RT Master Mix (Takara, RR047A).

    Article Title: Cisplatin exposure alters long-term metabolic phenotype of male, but not female, high-fat diet-fed mice
    Article Snippet: For liver samples, RNA was extracted from flash-frozen tissue using TRIzolTM reagent (Invitrogen, #15596018), as per manufacturer’s instructions. .. DNase treatment was performed prior to cDNA synthesis using the iScript gDNA Clear cDNA synthesis Kit (Bio-Rad, #1725035). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, #1725271) and run on a CFX384 (Bio-Rad). ..



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