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irfp stop renl sequence  (Addgene inc)


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    Structured Review

    Addgene inc irfp stop renl sequence
    Irfp Stop Renl Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/irfp+sequence/CAG-iRFP+(Plasmid+%23140460)/pmc07210380-176-36-41
    Average 90 stars, based on 1 article reviews
    irfp stop renl sequence - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Fate-restricted retinal progenitor cells adopt a molecular profile and spatial position distinct from multipotent progenitor cells
    Article Snippet: .. For the CAG-iRFP plasmid, the iRFP coding sequence was cloned from piRFP670-N1 vector (Addgene plasmid #45457) into the Stagia3 backbone, from which the GFP had been removed and the CAG promoter had been cloned upstream. ..

    Article Title: Poly(Beta-Amino Ester) Nanoparticles Enable Nonviral Delivery of CRISPR-Cas9 Plasmids for Gene Knockout and Gene Deletion
    Article Snippet: .. A sequence containing iRFP and transcription stop sequences was cloned into the PBCAG-EGFP plasmid backbone, and the ReNL gene was inserted into this plasmid using restriction enzyme cloning to create a PiggyBac transposon plasmid containing the iRFP-STOP-ReNL sequence (plasmid available on Addgene). ..

    Sequencing:

    Article Title: Fate-restricted retinal progenitor cells adopt a molecular profile and spatial position distinct from multipotent progenitor cells
    Article Snippet: .. For the CAG-iRFP plasmid, the iRFP coding sequence was cloned from piRFP670-N1 vector (Addgene plasmid #45457) into the Stagia3 backbone, from which the GFP had been removed and the CAG promoter had been cloned upstream. ..

    Article Title: Poly(Beta-Amino Ester) Nanoparticles Enable Nonviral Delivery of CRISPR-Cas9 Plasmids for Gene Knockout and Gene Deletion
    Article Snippet: .. A sequence containing iRFP and transcription stop sequences was cloned into the PBCAG-EGFP plasmid backbone, and the ReNL gene was inserted into this plasmid using restriction enzyme cloning to create a PiggyBac transposon plasmid containing the iRFP-STOP-ReNL sequence (plasmid available on Addgene). ..

    Clone Assay:

    Article Title: Fate-restricted retinal progenitor cells adopt a molecular profile and spatial position distinct from multipotent progenitor cells
    Article Snippet: .. For the CAG-iRFP plasmid, the iRFP coding sequence was cloned from piRFP670-N1 vector (Addgene plasmid #45457) into the Stagia3 backbone, from which the GFP had been removed and the CAG promoter had been cloned upstream. ..

    Article Title: Poly(Beta-Amino Ester) Nanoparticles Enable Nonviral Delivery of CRISPR-Cas9 Plasmids for Gene Knockout and Gene Deletion
    Article Snippet: .. A sequence containing iRFP and transcription stop sequences was cloned into the PBCAG-EGFP plasmid backbone, and the ReNL gene was inserted into this plasmid using restriction enzyme cloning to create a PiggyBac transposon plasmid containing the iRFP-STOP-ReNL sequence (plasmid available on Addgene). ..

    Cloning:

    Article Title: Poly(Beta-Amino Ester) Nanoparticles Enable Nonviral Delivery of CRISPR-Cas9 Plasmids for Gene Knockout and Gene Deletion
    Article Snippet: .. A sequence containing iRFP and transcription stop sequences was cloned into the PBCAG-EGFP plasmid backbone, and the ReNL gene was inserted into this plasmid using restriction enzyme cloning to create a PiggyBac transposon plasmid containing the iRFP-STOP-ReNL sequence (plasmid available on Addgene). ..



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    Addgene inc irfp stop renl sequence
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    Image Search Results


    HeLa cells expressing eMagA-mCherry-Mito (green) and Lys-eMagB-iRFP (magenta). Scale bar: 0.5 μm.

    Journal: eLife

    Article Title: Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells

    doi: 10.7554/eLife.63230

    Figure Lengend Snippet: HeLa cells expressing eMagA-mCherry-Mito (green) and Lys-eMagB-iRFP (magenta). Scale bar: 0.5 μm.

    Article Snippet: PH OSBP sequence was obtained from GFP-PH OSBP (Tim Levine, UCL Institute of Ophthalmology). iRFP-P4C was cloned amplifying the iRFP coding sequence piRFP670-N1 (Addgene plasmid # 45457) and inserted at AgeI and BsrGI cloning sites in GFP-P4C SidC (kind gift of Yuxin Mao, Cornell).

    Techniques:

    TagRFP-T-VAPB (1-218) -eMagB is shown on the left, iRFP-P4C is shown on the right. Scale bar: 5 μm.

    Journal: eLife

    Article Title: Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells

    doi: 10.7554/eLife.63230

    Figure Lengend Snippet: TagRFP-T-VAPB (1-218) -eMagB is shown on the left, iRFP-P4C is shown on the right. Scale bar: 5 μm.

    Article Snippet: PH OSBP sequence was obtained from GFP-PH OSBP (Tim Levine, UCL Institute of Ophthalmology). iRFP-P4C was cloned amplifying the iRFP coding sequence piRFP670-N1 (Addgene plasmid # 45457) and inserted at AgeI and BsrGI cloning sites in GFP-P4C SidC (kind gift of Yuxin Mao, Cornell).

    Techniques:

    TagRFP-T-VAPB (1-218) -eMagB is shown on the left, iRFP-P4C is shown on the right. Scale bar: 5 μm.

    Journal: eLife

    Article Title: Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells

    doi: 10.7554/eLife.63230

    Figure Lengend Snippet: TagRFP-T-VAPB (1-218) -eMagB is shown on the left, iRFP-P4C is shown on the right. Scale bar: 5 μm.

    Article Snippet: PH OSBP sequence was obtained from GFP-PH OSBP (Tim Levine, UCL Institute of Ophthalmology). iRFP-P4C was cloned amplifying the iRFP coding sequence piRFP670-N1 (Addgene plasmid # 45457) and inserted at AgeI and BsrGI cloning sites in GFP-P4C SidC (kind gift of Yuxin Mao, Cornell).

    Techniques:

    Journal: eLife

    Article Title: Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells

    doi: 10.7554/eLife.63230

    Figure Lengend Snippet:

    Article Snippet: PH OSBP sequence was obtained from GFP-PH OSBP (Tim Levine, UCL Institute of Ophthalmology). iRFP-P4C was cloned amplifying the iRFP coding sequence piRFP670-N1 (Addgene plasmid # 45457) and inserted at AgeI and BsrGI cloning sites in GFP-P4C SidC (kind gift of Yuxin Mao, Cornell).

    Techniques: Sequencing, Recombinant, Cloning, Generated, Software