healthy ipsc (Axol Bioscience)

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Axol Bioscience healthy ipsc
Healthy Ipsc, supplied by Axol Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ipsc+derived+cms/Human+iPSC-Derived+Ventricular+Cardiomyocytes/pmc12519157-68-2-8
Average 93 stars, based on 22 article reviews

healthy ipsc - by Bioz Stars, 2026-10

93/100 stars

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Cell Culture:

Article Title: Amelioration and treatment of infarction damage with a polypeptide segement of BNIP3
Article Snippet: Cell lines The HL-1 cell line was provided by W. C. Claycomb (Louisiana State University Medical Center, New Orleans, LA, USA) and cultured as previously described (Claycomb et al., 1998). .. Human CMs were obtained from Axol Bioscience, UK, and cultured according to the manufacturer's instructions. ..

In Vitro:

Article Title: Abstracts of the 83 rd Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT) and the 19 th Annual Meeting of the Association of the Clinical Pharmacology Germany (VKliPha) With contribution of the Arbeitsgemeinschaft für Angewandte Humanpharmakologie e. V. (AGAH).
Article Snippet: Structure IDs Oral presentations ............................................................................................................................................................. 1–100 Pharmacology – G–protein coupled receptors .................................................................................................................... 1–6 Pharmacology – Malignancies .......................................................................................................................................... 7–12 Pharmacology – Cardiac disease ................................................................................................................................... 13–18 Organ Toxicology ........................................................................................................................................................... 19–22 Endocrine effects and developmental toxicology ............................................................................................................. 23–26 Pharmacology – NO, CO and reactive oxygen species ................................................................................................... 27–31 Pharmacology – Central nervous system ........................................................................................................................ 32–36 Pharmacology – Endocrine system ................................................................................................................................. 37–41 Toxins ............................................................................................................................................................................. 42–46 Pharmacology – Cardiovascular Disease ....................................................................................................................... 47–52 Toxicology – Mutagenesis and carcinogenesis I ............................................................................................................. 53–56 Toxicology – Mutagenesis and carcinogenesis II ............................................................................................................ 57–60 From Bench to Bedside – Clinical Pharmacology ............................................................................................................ 61–66 Pharmacology – Cyclic nucleotides ................................................................................................................................ 67–72 4.

Expressing:

Article Title: FABP4 Enhances Lipidic and Fibrotic Cardiac Structural and Ca <sup>2+</sup> Dynamic Changes
Article Snippet: Circ Arrhythm Electrophysiol.. 2024;17:e012683.. DOI: 10.1161/CIRCEP.123.012683 September 2024 Correspondence to: Sonia Eiras, PhD, Laboratorio 6, IDIS, Planta-2.C/Choupana s/n, Complejo Hospitalario Universitario, de Santiago de Compostela, Santiago de Compostela 15706, A Coruña, Spain.

Article Title: Inhibition of Wnt/β-catenin signaling upregulates Na v 1.5 channels in Brugada syndrome iPSC-derived cardiomyocytes.
Article Snippet: To minimize well- to- well variations during the differentiation, cells at Day 14– 20 were lifted with TrypLE (Life Technologies, 12605- 036), pooled, and replated into new plates at a density of 250,000 cells/ cm2 in RPMI 1640 medium (Corning, 10- 040- CM) containing 1x B- 27 supplement (ThermoFisher, 17504044). .. An additional line of healthy iPSC- CMs (designated as Healthy Line 1) was purchased from Axolbio (Cambridge, UK, Catalogue No.: ax2505). iPSCs were derived from cord blood CD34+ cells collected from a healthy male newborn by reprogramming with episomal vectors expressing the reprogramming factors. .. An additional line of healthy iPSC- CMs (designated as Healthy Line 1) was purchased from Axolbio (Cambridge, UK, Catalogue No.: ax2505).

Real-time Polymerase Chain Reaction:

Immunocytochemistry:

Membrane:

Article Title: Reverse engineering of BNIP3 identifies a mitochondrial protective peptide
Article Snippet: H 2 O 2 generation was further determined in MCF-7 cells (15,000 cells per well, 2 μM STS, 52 μM B-017, 1 h) using the Invitrogen CellROX Deep Red Reagent (ThermoFisher Scientific, C10422 ), according to the manufacturer’s instructions, using the BMG FLUOstar Omega Microplate Reader. .. To analyse the mitochondrial inner membrane potential in human iPSC-derived cardiomyocytes (Axol 2505), the cells were incubated in HEPES buffer (113 mM NaCl, 4.7 mM KCl, 12 mM HEPES, 1.2 mM MgSO 4 , 30 mM taurine, and 1.3 mM CaCl 2 , pH 7.4) under 1% O 2 at 37 °C for 2 h. For reoxygenation, the cells were incubated in HEPES buffer supplemented with 5.5 mM glucose under 21% O 2 at 37 °C for 2 h. During hypoxia/reoxygenation the cardiomyocytes were treated with B-017 (4.4 μM) or the negative control peptide (4.4 μM). .. To determine the effect of B-017 on STS-induced changes in mitochondrial inner membrane potential, MCF-7 cells (15,000 cells per well) were incubated with 500 nM STS and 52 μM B-017 for 1 h. To analyse the effect of B-017 on doxorubicin-induced changes in mitochondrial inner membrane potential, human cardiomyocytes (7,500 cells per well HCM, PromoCell) were incubated with 10 μM DOX and 4.4 μM B-017 for 4 h. The iPSC-derived cardiomyocytes and MCF-7 cells were then incubated with 6 μM JC-1 dye in medium at 37 °C for 30 min and HCM with TMRM dye and processed according to the manufacturer’s instructions (JC-1 Kit, ThermoFisher Scientific, TMRM Kit, Abcam).

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