2 d ge buffer (Cytiva Europe)

Cytiva Europe is a verified supplier

Cytiva Europe manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cytiva Europe 2 d ge buffer
2 D Ge Buffer, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 97/100, based on 2727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ipg+buffer/IPG+Buffer+pH+3-10/pmc03118829-73-41-42
Average 97 stars, based on 2727 article reviews

2 d ge buffer - by Bioz Stars, 2026-10

97/100 stars

Images

other:

Article Title: Identification by Reverse Vaccinology of Three Virulence Factors in Burkholderia cenocepacia That May Represent Ideal Vaccine Antigens
Article Snippet: Pellets were then resuspended in 125 μL of 8M Urea, 4% CHAPS, 0.1 M Dithioerythritol (UCD), and 0.625 μL of 0.5% bromophenol blue and 0.7 μL of IPG buffer pH 3–10 (GE-Healthcare, Chicago, IL, USA) were added.

Article Title: Altered Protein Networks and Cellular Pathways in Severe West Nile Disease in Mice
Article Snippet: N-hydroxy succinimide ester Cy2, Cy3 and Cy5, urea, glycerol, mineral oil, immobiline DryStrip gel (18 cm, pH = 3–10, pH = 4–7 and pH = 6–11) and IPG buffer solutions (pH = 3–10, 4–7 and 6–11) were purchased from GE Healthcare (Piscataway, NJ).

Article Title: Carbonylated Proteins as Key Regulators in the Progression of Metabolic Syndrome
Article Snippet: Equal protein amounts of Cy2-, Cy3-, and Cy5-labeled samples were mixed and added to an equal volume of 2 × sample buffer (7 M urea, 2 M thiourea, 4% CHAPS, 130 mM dithiothreitol (DTT), 2% IPG buffer (pI 3–10; GE Healthcare), and a cocktail of protease inhibitors).

Article Title: Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in Saccharomyces cerevisiae
Article Snippet: Proteins were mixed with 350 μL rehydration solution (7 M urea, 2 M Thiourea, 2% 3-[(3-cholamidopropyl) dimethylammonio]- 1-propane-sulfonate (CHAPS) (Sigma-Aldrich), 40 mM Tris (Amersham, Piscataway, NJ, USA), 0.002% bromophenol blue dye (BPB)), 1% DTT (Duchefa Biochemie, Haarlem, Netherlands), and 0.05% immobilized pH gradient (IPG) buffer 3-10 (GE Healthcare, Piscataway, NJ, USA).

Purification:

Article Title: Identification of novel serum autoantibody biomarkers for early esophageal squamous cell carcinoma and high-grade intraepithelial neoplasia detection
Article Snippet: The protein concentration was determined using a 2-D Quant kit (GE, USA), and the extracted protein was purified using a 2-D Clean-up kit (GE). .. The hydration solution (124 μL) was equilibrated at room temperature (27°C) in advance and 1 μL immobilized pH gradient (IPG) buffer (pH 3–10, GE) was added to the purified protein sample. ..

Suspension:

Article Title: Proteome Analysis of Cultivated Vascular Smooth Muscle Cells from a CADASIL Patient
Article Snippet: The resulted cell debris was discarded and the total protein amount in the supernatant was measured by 2-D Quant kit (Amersham Biosciences Co, San Francisco, CA, USA). .. We mixed 50 μg (for analytical gels) or 100–150 μg (preparative gels) of protein suspension with rehydration buffer containing 7 M urea, 2 M thiourea, 4% CHAPS, 1% IPG-buffer (pH 3–10 or pH 4–7, Amersham Biosciences AB, Uppsala, Sweden), and 1% DTT. .. The IEF was performed on an IPGphor (Amersham Pharmacia Biotech UK Ltd) with 18 cm IPG-strips, pH-range 3–10 or 4–7 (Amersham Biosciences AB).

Stripping Membranes:

Article Title: Proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl
Article Snippet: Protein concentration was determined using the PlusOne 2-D Quant kit (GE Healthcare, Italy). .. For each gel, 300 μg of total protein were dissolved to a final volume of 250 μL in the re-hydration solution (5 M urea, 2 M thiourea, 2% CHAPS, 2% Zwittergent, 100 mM DeStreak and 0.5% IPG buffer pH 3–10 linear, GE Healthcare, Italy), and then applied on immobilized pH 3–10 linear gradient strips (IPG strip, GE Healthcare, Italy). .. The strips were hydrated on an IPGphor apparatus (GE Healthcare, Italy) for 16 h at 30 V/h, then focused for 26 h until 50,000 Vhr.

Review