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1 × ip1 stimulation buffer  (Cisbio Bioassays)

 
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    Cisbio Bioassays 1 × ip1 stimulation buffer
    1 × Ip1 Stimulation Buffer, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ip1+stimulation+buffer/ip1+stimulation+buf/pm32648604-33-17-20
    Average 90 stars, based on 1 article reviews
    1 × ip1 stimulation buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Stable Transfection:

    Article Title: Heterocyclic compound
    Article Snippet: To a 384-well white plate (Greiner) was added 4 μL of the test compound diluted with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA containing 80 nM acetylcholine. .. Freeze stock of CHO-K1 cells stably expressing human M1 receptor (hCHRM1) was prepared with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA, 4 μL thereof was added (10,000 cells/well) and the mixture was cultured in a 5% CO2 incubator at 37° C. for 1 hr. .. 4 μL of a solution (CisBio) containing IP1-d2 and Anti-IP1-cryptate Tb conjugate was added, the mixture was incubated at room temperature for 1 hr, and the time-resolved fluorescence signal was measured on an Envision plate reader (PerkinElmer).

    Article Title: Heterocyclic compound
    Article Snippet: To a 384-well white plate (Greiner) was added 4 μL of the test compound diluted with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA containing 80 nM acetylcholine. .. Freeze stock of CHO-K1 cells stably expressing human M1 receptor (hCHRM1) was prepared with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA, 4 μL thereof was added (10,000 cells/well) and the mixture was cultured in a 5% CO2 incubator at 37° C. for 1 hr. .. 4 μL of a solution (CisBio) containing IP1-d2 and Anti-IP1-cryptate Tb conjugate was added, the mixture was incubated at room temperature for 1 hr, and the time-resolved fluorescence signal was measured on an Envision plate reader (PerkinElmer).

    Expressing:

    Article Title: Heterocyclic compound
    Article Snippet: To a 384-well white plate (Greiner) was added 4 μL of the test compound diluted with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA containing 80 nM acetylcholine. .. Freeze stock of CHO-K1 cells stably expressing human M1 receptor (hCHRM1) was prepared with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA, 4 μL thereof was added (10,000 cells/well) and the mixture was cultured in a 5% CO2 incubator at 37° C. for 1 hr. .. 4 μL of a solution (CisBio) containing IP1-d2 and Anti-IP1-cryptate Tb conjugate was added, the mixture was incubated at room temperature for 1 hr, and the time-resolved fluorescence signal was measured on an Envision plate reader (PerkinElmer).

    Article Title: Heterocyclic compound
    Article Snippet: To a 384-well white plate (Greiner) was added 4 μL of the test compound diluted with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA containing 80 nM acetylcholine. .. Freeze stock of CHO-K1 cells stably expressing human M1 receptor (hCHRM1) was prepared with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA, 4 μL thereof was added (10,000 cells/well) and the mixture was cultured in a 5% CO2 incubator at 37° C. for 1 hr. .. 4 μL of a solution (CisBio) containing IP1-d2 and Anti-IP1-cryptate Tb conjugate was added, the mixture was incubated at room temperature for 1 hr, and the time-resolved fluorescence signal was measured on an Envision plate reader (PerkinElmer).

    Cell Culture:

    Article Title: Heterocyclic compound
    Article Snippet: To a 384-well white plate (Greiner) was added 4 μL of the test compound diluted with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA containing 80 nM acetylcholine. .. Freeze stock of CHO-K1 cells stably expressing human M1 receptor (hCHRM1) was prepared with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA, 4 μL thereof was added (10,000 cells/well) and the mixture was cultured in a 5% CO2 incubator at 37° C. for 1 hr. .. 4 μL of a solution (CisBio) containing IP1-d2 and Anti-IP1-cryptate Tb conjugate was added, the mixture was incubated at room temperature for 1 hr, and the time-resolved fluorescence signal was measured on an Envision plate reader (PerkinElmer).

    Article Title: Heterocyclic compound
    Article Snippet: To a 384-well white plate (Greiner) was added 4 μL of the test compound diluted with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA containing 80 nM acetylcholine. .. Freeze stock of CHO-K1 cells stably expressing human M1 receptor (hCHRM1) was prepared with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA, 4 μL thereof was added (10,000 cells/well) and the mixture was cultured in a 5% CO2 incubator at 37° C. for 1 hr. .. 4 μL of a solution (CisBio) containing IP1-d2 and Anti-IP1-cryptate Tb conjugate was added, the mixture was incubated at room temperature for 1 hr, and the time-resolved fluorescence signal was measured on an Envision plate reader (PerkinElmer).

    Transfection:

    Article Title: The intramolecular agonist is obligate for activation of glycoprotein hormone receptors.
    Article Snippet: To perform the assay, Cisbio's IP-One Tb kit (Cisbio, Codolet, France) was used. .. Forty-eight hours after transfection, cells were stimulated for 1 hour at 37°C with 35 μL 1 × IP1 stimulation buffer (Cisbio) containing the respective reagents. .. Cells were lysed by adding 30 μL lysis buffer (Cisbio) per well and kept frozen at −20°C until measurement.

    Article Title: Mapping the Binding Sites of UDP and Prostaglandin E2 Glyceryl Ester in the Nucleotide Receptor P2Y 6
    Article Snippet: .. Then, 48 h after transfection, cells were stimulated 30 min at 37 °C with 35 μl 1× IP 1 stimulation buffer (Cisbio) containing the respective reagents (concentrations as indicated). .. Next, cells were lysed by adding 30 μl lysis buffer (Cisbio) per well and kept frozen at −20 °C until measurement.



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