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internal reflection fluorescence tirf microscope  (Nikon)


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    Nikon internal reflection fluorescence tirf microscope
    Internal Reflection Fluorescence Tirf Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/internal+reflection+fluorescence+microscope/Objectives/pmc12830645-283-9-14
    Average 99 stars, based on 59970 article reviews
    internal reflection fluorescence tirf microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Live Cell Imaging:

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine
    Article Snippet: The enteroids were imaged using a Zeiss LSM800 confocal microscope equipped with a 20x objective and a 40x immersion oil objective. .. Live-cell imaging was performed on a custom-built microscope based on an Eclipse Ti2 body (Nikon), using a 10x/0.45 Plan Apo Lambda air objective (Nikon), and a back-lit sCMOS camera with a physical pixel size of 11 μm (Prime 95B, Photometrics), and a final image pixel size of 1.12 μm. ..

    Microscopy:

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine
    Article Snippet: The enteroids were imaged using a Zeiss LSM800 confocal microscope equipped with a 20x objective and a 40x immersion oil objective. .. Live-cell imaging was performed on a custom-built microscope based on an Eclipse Ti2 body (Nikon), using a 10x/0.45 Plan Apo Lambda air objective (Nikon), and a back-lit sCMOS camera with a physical pixel size of 11 μm (Prime 95B, Photometrics), and a final image pixel size of 1.12 μm. ..

    Article Title: Comparative analysis of select 3D human mesenchymal stromal cell-derived spheroid models for adipogenic differentiation
    Article Snippet: .. Brightfield images were acquired using Epi-Fluorescence Eclipse TE300 microscope, equipped with a 10x objective (Nikon, Tokyo, Japan), and analysed with ImageJ Fiji software (National Institutes of Health, USA) to quantify spheroid size and morphology. ..

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Article Title: A Nitric Oxide–Based STING Agonist Nanoadjuvant With ICD‐Derived Membrane Antigens Unleashes Robust Antitumor Immunity
    Article Snippet: .. Imaging was performed on a Nikon A1R HD25 confocal microscope using a 60× oil objective. ..

    Article Title: Sex‐Specific Effects of Dexmedetomidine on Brain Microvascular Endothelial Cell Function After Traumatic Brain Injury in Juvenile Mice
    Article Snippet: Cells were then incubated with BioTracker 633 Red Mitochondria Dye (100 nM; MilliporeSigma, MA, USA) and BioTracker Mitochondrial Lipid Peroxide Live Cell Ferroptosis Indicator (100 nM; MilliporeSigma, MA, USA) for 30 min before imaging. .. Images were acquired using a Nikon Plan Apo 20×/0.9 objective mounted on a Nikon Eclipse TS2R fluorescence microscope (Nikon, NY, USA). ..

    Brightfield:

    Article Title: Comparative analysis of select 3D human mesenchymal stromal cell-derived spheroid models for adipogenic differentiation
    Article Snippet: .. Brightfield images were acquired using Epi-Fluorescence Eclipse TE300 microscope, equipped with a 10x objective (Nikon, Tokyo, Japan), and analysed with ImageJ Fiji software (National Institutes of Health, USA) to quantify spheroid size and morphology. ..

    Article Title: Gasdermin D-mediated delivery of caspase inhibitors to suppress pyroptosis.
    Article Snippet: Caspase-1, -4, -5 and -11 activate gasdermin D (GSDMD) pores, causing pyroptotic cell death and the release of the interleukins IL-1β and IL-18 (ref. 1).. Blocking this pathway holds therapeutic promise for the treatment of inflammatory disorders, but cellpermeable caspase inhibitors have not proved successful in clinical trials.. Here we describe covalent caspase inhibitors that selectively block pyroptosis and IL-1β secretion despite being excluded from healthy cells.

    Software:

    Article Title: Comparative analysis of select 3D human mesenchymal stromal cell-derived spheroid models for adipogenic differentiation
    Article Snippet: .. Brightfield images were acquired using Epi-Fluorescence Eclipse TE300 microscope, equipped with a 10x objective (Nikon, Tokyo, Japan), and analysed with ImageJ Fiji software (National Institutes of Health, USA) to quantify spheroid size and morphology. ..

    Article Title: O‐GlcNAcylation Stimulation Modulates Na+/K+ ATPase and Improves Outcomes in Hemorrhagic Shock
    Article Snippet: .. Images were acquired using a Nikon Eclipse Ti2 inverted microscope equipped with a Nikon DS‐Qi2 digital camera using a 40× objective and were analyzed using Image J software (V1.53U version). ..

    Morphology:

    Article Title: Comparative analysis of select 3D human mesenchymal stromal cell-derived spheroid models for adipogenic differentiation
    Article Snippet: .. Brightfield images were acquired using Epi-Fluorescence Eclipse TE300 microscope, equipped with a 10x objective (Nikon, Tokyo, Japan), and analysed with ImageJ Fiji software (National Institutes of Health, USA) to quantify spheroid size and morphology. ..

    Single Cell:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Clone Assay:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Process/Product Development:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Fluorescence:

    Article Title: Gasdermin D-mediated delivery of caspase inhibitors to suppress pyroptosis.
    Article Snippet: Caspase-1, -4, -5 and -11 activate gasdermin D (GSDMD) pores, causing pyroptotic cell death and the release of the interleukins IL-1β and IL-18 (ref. 1).. Blocking this pathway holds therapeutic promise for the treatment of inflammatory disorders, but cellpermeable caspase inhibitors have not proved successful in clinical trials.. Here we describe covalent caspase inhibitors that selectively block pyroptosis and IL-1β secretion despite being excluded from healthy cells.

    Article Title: Sex‐Specific Effects of Dexmedetomidine on Brain Microvascular Endothelial Cell Function After Traumatic Brain Injury in Juvenile Mice
    Article Snippet: Cells were then incubated with BioTracker 633 Red Mitochondria Dye (100 nM; MilliporeSigma, MA, USA) and BioTracker Mitochondrial Lipid Peroxide Live Cell Ferroptosis Indicator (100 nM; MilliporeSigma, MA, USA) for 30 min before imaging. .. Images were acquired using a Nikon Plan Apo 20×/0.9 objective mounted on a Nikon Eclipse TS2R fluorescence microscope (Nikon, NY, USA). ..

    Spinning Disk:

    Article Title: Gasdermin D-mediated delivery of caspase inhibitors to suppress pyroptosis.
    Article Snippet: Caspase-1, -4, -5 and -11 activate gasdermin D (GSDMD) pores, causing pyroptotic cell death and the release of the interleukins IL-1β and IL-18 (ref. 1).. Blocking this pathway holds therapeutic promise for the treatment of inflammatory disorders, but cellpermeable caspase inhibitors have not proved successful in clinical trials.. Here we describe covalent caspase inhibitors that selectively block pyroptosis and IL-1β secretion despite being excluded from healthy cells.

    Confocal:

    Article Title: Gasdermin D-mediated delivery of caspase inhibitors to suppress pyroptosis.
    Article Snippet: Caspase-1, -4, -5 and -11 activate gasdermin D (GSDMD) pores, causing pyroptotic cell death and the release of the interleukins IL-1β and IL-18 (ref. 1).. Blocking this pathway holds therapeutic promise for the treatment of inflammatory disorders, but cellpermeable caspase inhibitors have not proved successful in clinical trials.. Here we describe covalent caspase inhibitors that selectively block pyroptosis and IL-1β secretion despite being excluded from healthy cells.

    Article Title: A Nitric Oxide–Based STING Agonist Nanoadjuvant With ICD‐Derived Membrane Antigens Unleashes Robust Antitumor Immunity
    Article Snippet: .. Imaging was performed on a Nikon A1R HD25 confocal microscope using a 60× oil objective. ..

    Inverted Microscopy:

    Article Title: O‐GlcNAcylation Stimulation Modulates Na+/K+ ATPase and Improves Outcomes in Hemorrhagic Shock
    Article Snippet: .. Images were acquired using a Nikon Eclipse Ti2 inverted microscope equipped with a Nikon DS‐Qi2 digital camera using a 40× objective and were analyzed using Image J software (V1.53U version). ..

    Imaging:

    Article Title: A Nitric Oxide–Based STING Agonist Nanoadjuvant With ICD‐Derived Membrane Antigens Unleashes Robust Antitumor Immunity
    Article Snippet: .. Imaging was performed on a Nikon A1R HD25 confocal microscope using a 60× oil objective. ..

    Phase Contrast:

    Article Title: Polyphasic taxonomic description of Micromonospora aquaegermanicae sp. nov. and Plantactinospora tunisiensis sp. nov.
    Article Snippet: A colony of strain JZT32T was streaked onto Glucose-Yeast ExtractMalt Extract agar (GYM, DSMZ 65) and incubated at 28 °C for 14 days. .. The purity of the strain was checked using phase-contrast light microscopy without staining (Nikon Eclipse, 100x objective lens). ..

    Light Microscopy:

    Article Title: Polyphasic taxonomic description of Micromonospora aquaegermanicae sp. nov. and Plantactinospora tunisiensis sp. nov.
    Article Snippet: A colony of strain JZT32T was streaked onto Glucose-Yeast ExtractMalt Extract agar (GYM, DSMZ 65) and incubated at 28 °C for 14 days. .. The purity of the strain was checked using phase-contrast light microscopy without staining (Nikon Eclipse, 100x objective lens). ..

    Staining:

    Article Title: Polyphasic taxonomic description of Micromonospora aquaegermanicae sp. nov. and Plantactinospora tunisiensis sp. nov.
    Article Snippet: A colony of strain JZT32T was streaked onto Glucose-Yeast ExtractMalt Extract agar (GYM, DSMZ 65) and incubated at 28 °C for 14 days. .. The purity of the strain was checked using phase-contrast light microscopy without staining (Nikon Eclipse, 100x objective lens). ..



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    Image Search Results


    DNA-PAINT experiment on single gold nanospheres. (a) Schematic of a gold nanosphere sample on a glass substrate excited with an epi-illumination fluorescence microscope. Fluorescent intensity time traces are recorded at 10 frames per second, showing bursts with an event typically lasting for 10 frames. Each fluorescent event, averaged over all frames above the threshold, provides a polarized PSF image. Four example events with the extracted α and DoLP indicate single-emitter binding to a particle at different locations. Scale bar of 200 nm applies to all PSFs. (b) Scatter plot of DoLP and α apparent for fluorescence events on six single nanospheres. (c) DoLP as a function of integrated fluorescence intensity showing that brighter events exhibit a higher DoLP because they occur at the equator of the particle.

    Journal: Nanophotonics

    Article Title: Single-shot Stokes polarimetry of plasmon-coupled single-molecule fluorescence

    doi: 10.1515/nanoph-2025-0352

    Figure Lengend Snippet: DNA-PAINT experiment on single gold nanospheres. (a) Schematic of a gold nanosphere sample on a glass substrate excited with an epi-illumination fluorescence microscope. Fluorescent intensity time traces are recorded at 10 frames per second, showing bursts with an event typically lasting for 10 frames. Each fluorescent event, averaged over all frames above the threshold, provides a polarized PSF image. Four example events with the extracted α and DoLP indicate single-emitter binding to a particle at different locations. Scale bar of 200 nm applies to all PSFs. (b) Scatter plot of DoLP and α apparent for fluorescence events on six single nanospheres. (c) DoLP as a function of integrated fluorescence intensity showing that brighter events exhibit a higher DoLP because they occur at the equator of the particle.

    Article Snippet: DNA-PAINT measurements were performed on an inverted total internal reflection fluorescence microscope (Nikon) with a 100×/NA 1.49 objective.

    Techniques: Fluorescence, Microscopy, Binding Assay

    a Time-lapse TIRF microscopy images of debranching events in the absence (left) or presence (right) of Coro7 FL. The flow rate is 200 µL min − 1 , corresponding to an average force of ~0.45 pN on an average ~2 µm-long branch . b Time courses of branch dissociation in the absence or presence of Coro7 FL. Solid lines through the data represent best fits to single (without Coro7 FL, black) or double (with Coro7 FL, blue shades) exponentials. c Debranching rate constants as a function of Coro7 FL concentration. The individual data points represent the observed debranching rate constants obtained from the best fits of the time courses shown in ( b ) to double exponentials. The teal and cyan horizontal lines represent the best global fits of the debranching time courses shown in ( b ) to a double exponential with a single rate constant for each phase (fast and slow). d Dependence of the fast-debranching phase amplitudes on Coro7 FL concentration. The blue circles represent the amplitudes obtained from individual fitting of the time courses shown in ( b ) to double exponentials, and the red circles represent the relative amplitudes obtained from global fitting. The solid line through the data represents the best fit to a rectangular hyperbola, yielding an apparent Coro7 FL binding affinity of 31 ± 1 nM for both individually and globally fitted parameters. e – g Time courses of branch dissociation in the absence or presence of various concentrations of Coro7 construct β2CA (solid lines indicate double-exponential fits) and constructs β1β2 and CA (solid lines indicate single-exponential fits). The graphs in ( b , e – g ) show averages; each condition was repeated two to four times, with an average of 43 fields of view, 113 mother filaments, and 155 branches analyzed per condition. In ( c and d ), the error bars represent standard deviation (SD) of the best-fit parameters from the time courses shown in ( b ), as calculated using Origin software. Source data are provided in the Source Data file.

    Journal: Nature Communications

    Article Title: Mechanism of Arp2/3 complex branch disassembly by human Coro7

    doi: 10.1038/s41467-025-64787-z

    Figure Lengend Snippet: a Time-lapse TIRF microscopy images of debranching events in the absence (left) or presence (right) of Coro7 FL. The flow rate is 200 µL min − 1 , corresponding to an average force of ~0.45 pN on an average ~2 µm-long branch . b Time courses of branch dissociation in the absence or presence of Coro7 FL. Solid lines through the data represent best fits to single (without Coro7 FL, black) or double (with Coro7 FL, blue shades) exponentials. c Debranching rate constants as a function of Coro7 FL concentration. The individual data points represent the observed debranching rate constants obtained from the best fits of the time courses shown in ( b ) to double exponentials. The teal and cyan horizontal lines represent the best global fits of the debranching time courses shown in ( b ) to a double exponential with a single rate constant for each phase (fast and slow). d Dependence of the fast-debranching phase amplitudes on Coro7 FL concentration. The blue circles represent the amplitudes obtained from individual fitting of the time courses shown in ( b ) to double exponentials, and the red circles represent the relative amplitudes obtained from global fitting. The solid line through the data represents the best fit to a rectangular hyperbola, yielding an apparent Coro7 FL binding affinity of 31 ± 1 nM for both individually and globally fitted parameters. e – g Time courses of branch dissociation in the absence or presence of various concentrations of Coro7 construct β2CA (solid lines indicate double-exponential fits) and constructs β1β2 and CA (solid lines indicate single-exponential fits). The graphs in ( b , e – g ) show averages; each condition was repeated two to four times, with an average of 43 fields of view, 113 mother filaments, and 155 branches analyzed per condition. In ( c and d ), the error bars represent standard deviation (SD) of the best-fit parameters from the time courses shown in ( b ), as calculated using Origin software. Source data are provided in the Source Data file.

    Article Snippet: Direct visualization of actin filaments and branches was performed using a Nikon Eclipse Ti2 total internal reflection fluorescence (TIRF) microscope equipped with an SR HP Apo TIRF ×100 objective (Nikon) and an Andor iXon897 EMCCD camera.

    Techniques: Microscopy, Concentration Assay, Binding Assay, Construct, Standard Deviation, Software