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odyssey blocking buffer  (LI-COR)


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    Structured Review

    LI-COR odyssey blocking buffer
    Odyssey Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intercept+tbs+blocking+buffer/TBS+BLOCKING+BUFFER+AND+PVDF+KIT/pm37752346-99-10-13
    Average 99 stars, based on 183 article reviews
    odyssey blocking buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Substituted 6,7-dihydro-5H-benzo[7]annulene compounds and their derivatives, processes for their preparation and therapeutic uses thereof
    Article Snippet: MCF7 cells (ATCC) were seeded in 384 wells microplate (collagen coated) at a concentration of 10000 cells/30 μL per well in red phenol free MEM alpha medium (invitrogen) containing 5% charcoal dextran striped FBS. .. LI-COR blocking buffer was removed and cells were incubated overnight at cold room with 50 μL anti-ER rabbit monoclonal antibody (Thermo scientific MA1-39540) diluted at 1:1000 in LI-COR blocking buffer containing 0.1% tween-20. ..

    Article Title: Mutations outside the MR1 antigen binding groove differentially inhibit presentation of exogenous antigens
    Article Snippet: Supernatants were collected after centrifugation, and whole cell lysates were combined with loading buffer and reducing agent (both Invitrogen) before loading onto a 4 to 20% polyacrylamide gel (Bio-Rad). .. Gels were run at 120V for about 1h and transferred onto a polyvinylidene fluoride membrane (Millipore), which was then blocked in Odyssey blocking buffer (Li-Cor) for at least 1h at RT. ..

    Article Title: Methylglyoxal-derived glycated albumin enhances the stemness potential of invasive ductal carcinoma-derived breast cancer stem-like cell line KAIMRC1
    Article Snippet: .. Following five washes for 10 min each in TBS-0.1% Tween at RT, membranes were incubated for 1 h at RT with infrared fluorescent secondary antibodies diluted (1:5,000 dilution) in LI-COR Odyssey ® blocking buffer: IRDye 680RD (red)-conjugated goat anti-rabbit or IRDye 800RD (green)-conjugated goat anti-mouse (LI-COR Biosciences) with continuous mixing. .. After washing, protein bands were visualized using the LI-COR Odyssey CLx Scanner, and protein expression levels were quantified using ImageJ software version 1.53e ( https://imagej.net/ij/index.html ) as previously described ( , ).

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis.
    Article Snippet: .. The fixed cells were quenched with 50 mM NH 4 Cl for 1 h and permeabilized with PBS containing 0.1% Triton X-100 for 15 min, prior to blocking with TBS Blocking Buffer (LI-COR Bioscience) for 1 h at room temperature. .. For arginase detection, a rabbit anti-arginase was used as primary antibody [21] (1:500 in blocking buffer) for 1 h, followed by an anti-rabbit AlexaFluor 546 (Life Technologies) (1:500) as secondary antibody.

    Article Title: Lactate and histone H3K18 lactylation are associated with metabolic control of gene expression in the retina.
    Article Snippet: Histone proteins were separated using 4–15% SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore). .. Blotted membranes were blocked in Licor blocking buffer and incubated with primary antibodies at 4 °C overnight anti-Pan lactyl (Rabbit, PTM1401, PTM Bio, China), anti-H3K18La (Rabbit, PTM1406RM, PTM Bio, China), anti- H4K12La (Rabbit, PTM101, PTM Bio, China), anti-H3K27Ac (Rabbit ab4729, Abcam, Cambridge, UK), anti-Histone H3 (Rabbit, ab176842, Abcam, Cambridge, UK). ..

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis.
    Article Snippet: .. Equal protein amounts (20 μg per lane) were resolved by SDS-PAGE and analyzed by western blot using anti-SODA (1:500) [50], anti-SODB (1:500) [50], anti-FLAG M2 (1:4,000, for Cas9 detection; F1804-MG, Sigma-Aldrich), anti-alpha Tubulin (1:5,000, loading control; 62204, Thermo Fisher) and anti-arginase (1:500, loading control) [21] antibodies diluted in TBS Blocking Buffer (LI-COR PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0014141 April 2, 2026 14 / 19 Bioscience). .. After primary incubation, membranes were washed three times in TBST (TBS, 0.1% Tween-20) and incubated for 1 h at room temperature, protected from light, with IRDye 800CW goat anti-Rabbit or IRDye 680RD anti-Mouse (LICORbio; 1:10,000 in TBS Blocking Buffer).

    Incubation:

    Article Title: Substituted 6,7-dihydro-5H-benzo[7]annulene compounds and their derivatives, processes for their preparation and therapeutic uses thereof
    Article Snippet: MCF7 cells (ATCC) were seeded in 384 wells microplate (collagen coated) at a concentration of 10000 cells/30 μL per well in red phenol free MEM alpha medium (invitrogen) containing 5% charcoal dextran striped FBS. .. LI-COR blocking buffer was removed and cells were incubated overnight at cold room with 50 μL anti-ER rabbit monoclonal antibody (Thermo scientific MA1-39540) diluted at 1:1000 in LI-COR blocking buffer containing 0.1% tween-20. ..

    Article Title: Methylglyoxal-derived glycated albumin enhances the stemness potential of invasive ductal carcinoma-derived breast cancer stem-like cell line KAIMRC1
    Article Snippet: .. Following five washes for 10 min each in TBS-0.1% Tween at RT, membranes were incubated for 1 h at RT with infrared fluorescent secondary antibodies diluted (1:5,000 dilution) in LI-COR Odyssey ® blocking buffer: IRDye 680RD (red)-conjugated goat anti-rabbit or IRDye 800RD (green)-conjugated goat anti-mouse (LI-COR Biosciences) with continuous mixing. .. After washing, protein bands were visualized using the LI-COR Odyssey CLx Scanner, and protein expression levels were quantified using ImageJ software version 1.53e ( https://imagej.net/ij/index.html ) as previously described ( , ).

    Article Title: Lactate and histone H3K18 lactylation are associated with metabolic control of gene expression in the retina.
    Article Snippet: Histone proteins were separated using 4–15% SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore). .. Blotted membranes were blocked in Licor blocking buffer and incubated with primary antibodies at 4 °C overnight anti-Pan lactyl (Rabbit, PTM1401, PTM Bio, China), anti-H3K18La (Rabbit, PTM1406RM, PTM Bio, China), anti- H4K12La (Rabbit, PTM101, PTM Bio, China), anti-H3K27Ac (Rabbit ab4729, Abcam, Cambridge, UK), anti-Histone H3 (Rabbit, ab176842, Abcam, Cambridge, UK). ..

    Membrane:

    Article Title: Mutations outside the MR1 antigen binding groove differentially inhibit presentation of exogenous antigens
    Article Snippet: Supernatants were collected after centrifugation, and whole cell lysates were combined with loading buffer and reducing agent (both Invitrogen) before loading onto a 4 to 20% polyacrylamide gel (Bio-Rad). .. Gels were run at 120V for about 1h and transferred onto a polyvinylidene fluoride membrane (Millipore), which was then blocked in Odyssey blocking buffer (Li-Cor) for at least 1h at RT. ..

    other:

    Article Title: Oxidative DNA damage drives apoptotic photoreceptor loss in NMNAT1-associated inherited retinal degeneration: a therapeutic opportunity.
    Article Snippet: Protein concentrations were determined using a BCA assay (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Substituted 6,7-dihydro-5H-benzo[7]annulene compounds and their derivatives, processes for their preparation and therapeutic uses thereof
    Article Snippet: MCF7 cells (ATCC) were seeded in 384 wells microplate (collagen coated) at a concentration of 10000 cells/30 μL per well in red phenol free MEM alpha medium (invitrogen) containing 5% charcoal dextran striped FBS.

    SDS Page:

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis.
    Article Snippet: .. Equal protein amounts (20 μg per lane) were resolved by SDS-PAGE and analyzed by western blot using anti-SODA (1:500) [50], anti-SODB (1:500) [50], anti-FLAG M2 (1:4,000, for Cas9 detection; F1804-MG, Sigma-Aldrich), anti-alpha Tubulin (1:5,000, loading control; 62204, Thermo Fisher) and anti-arginase (1:500, loading control) [21] antibodies diluted in TBS Blocking Buffer (LI-COR PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0014141 April 2, 2026 14 / 19 Bioscience). .. After primary incubation, membranes were washed three times in TBST (TBS, 0.1% Tween-20) and incubated for 1 h at room temperature, protected from light, with IRDye 800CW goat anti-Rabbit or IRDye 680RD anti-Mouse (LICORbio; 1:10,000 in TBS Blocking Buffer).

    Western Blot:

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis.
    Article Snippet: .. Equal protein amounts (20 μg per lane) were resolved by SDS-PAGE and analyzed by western blot using anti-SODA (1:500) [50], anti-SODB (1:500) [50], anti-FLAG M2 (1:4,000, for Cas9 detection; F1804-MG, Sigma-Aldrich), anti-alpha Tubulin (1:5,000, loading control; 62204, Thermo Fisher) and anti-arginase (1:500, loading control) [21] antibodies diluted in TBS Blocking Buffer (LI-COR PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0014141 April 2, 2026 14 / 19 Bioscience). .. After primary incubation, membranes were washed three times in TBST (TBS, 0.1% Tween-20) and incubated for 1 h at room temperature, protected from light, with IRDye 800CW goat anti-Rabbit or IRDye 680RD anti-Mouse (LICORbio; 1:10,000 in TBS Blocking Buffer).

    Control:

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis.
    Article Snippet: .. Equal protein amounts (20 μg per lane) were resolved by SDS-PAGE and analyzed by western blot using anti-SODA (1:500) [50], anti-SODB (1:500) [50], anti-FLAG M2 (1:4,000, for Cas9 detection; F1804-MG, Sigma-Aldrich), anti-alpha Tubulin (1:5,000, loading control; 62204, Thermo Fisher) and anti-arginase (1:500, loading control) [21] antibodies diluted in TBS Blocking Buffer (LI-COR PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0014141 April 2, 2026 14 / 19 Bioscience). .. After primary incubation, membranes were washed three times in TBST (TBS, 0.1% Tween-20) and incubated for 1 h at room temperature, protected from light, with IRDye 800CW goat anti-Rabbit or IRDye 680RD anti-Mouse (LICORbio; 1:10,000 in TBS Blocking Buffer).



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