proteinree blocking buffer (LI-COR)

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LI-COR proteinree blocking buffer
Proteinree Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/intercept+(tbs)+blocking+buffer/Intercept+(TBS)+Protein-Free+Blocking+Buffer/pm42106918-458-3-6
Average 98 stars, based on 92 article reviews

proteinree blocking buffer - by Bioz Stars, 2026-11

98/100 stars

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Blocking Assay:

Article Title: Tyrosine phosphorylation of the scaffold protein IQGAP1 by VEGFR2 modulates cell proliferation.
Article Snippet: Glutathione-Sepharose beads 481 (Catalog no. GE17-5132-01) and Protein A-Sepharose beads (Catalog no. GE17-0780-01) were purchased 482 from Cytiva. .. Antibody diluent (Catalog no. 927-85003), blocking buffer (Catalog no. 927-80003), IRDye 483 Jo urn al Pr e-p roo f 20 800CW Donkey anti-rabbit IgG secondary antibody (Catalog no. 926-32213), and IRDye 680RD Donkey 484 anti-mouse IgG secondary antibody (Catalog no. 926-68072) were purchased from LI-COR Biotech. .. 485 Phosphate-buffered saline (PBS) (0.144 g/L potassium dihydrogen phosphate, 9 g/L sodium chloride and 486 0.795 g/L disodium phosphate, pH 7.40.1) was purchased from Corning (Catalog no. 21-040-CV).

Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH.
Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA,) antibodies for their expression and phosphorylation. .. Total RNA for massive sequencing (RNA-seq) and qPCR was isolated using the commercial DNA, RNA, and protein purification kit (Macherey-Nagel, Düren, Germany).

Article Title: Systemic Propagation of STING Signalling via Generation of Large Extracellular Vesicles.
Article Snippet: 0.5 μg/mL poly(dA:dT) (Invivogen, tlrl-patn), 1.0 μg/mL 2’3’-cGAMP (ApexBio, B8362), or 5.0 μg/mL 45-bp SD was transfected into 3.0 × 106 cells using Lipofectamine 3000 Invitrogen, L3000) according to the manufacturer’s instruction. .. For phospho-antibodies, Intercept Proteinree Blocking Buffer (LICORbio, 927–80001) was used for blockng. .. he GFP-expressing or NanoLuc-expressing MDA-MB-231 cell ines were generated by lentiviral transduction (Sigma–Aldrich, RFGFPV).

Article Title: The redundant protein synthesis gene Aimp1 challenges the canonical inverse relationship between translation and autophagy.
Article Snippet: .. Cells were then permeabilized with either 0.1% Triton X-100, for 5 minutes, or 0.05 (w/v)% saponin, for 30 minutes, diluted in Intercept Protein-Free Blocking Buffer (LI-COR, 927-80001). .. Cells were then permeabilized with either 0.1% Triton X-100, for 5 minutes, or 0.05 (w/v)% saponin, for 30 minutes, diluted in Intercept Protein-Free Blocking Buffer (LI-COR, 927-80001).

Incubation:

Article Title: Insulin Induces Outer Blood-Retinal Barrier Disruption via Downregulation of Claudin-19.
Article Snippet: .. Membranes were blocked for 1 hour (927-80001; LICORbio, Lincoln, NE, USA) at room temperature and then incubated with primary antibody claudin-19 (NBP1-59277, 1:500; Novus Biologicals) and βactin (3700s, 1:5000; Cell Signaling Technology, Danvers, MA, USA) overnight at 4°C. .. They were then incubated with secondary antibody (IRDye, 1:10,000; LICORbio) for 1 hour at room temperature and imaged using the Odyssey cLX Imager (LICORbio).

Article Title: Insulin Induces Outer Blood–Retinal Barrier Disruption via Downregulation of Claudin-19
Article Snippet: .. Membranes were blocked for 1 hour (927-80001; LICORbio, Lincoln, NE, USA) at room temperature and then incubated with primary antibody claudin-19 (NBP1-59277, 1:500; Novus Biologicals) and β-actin (3700s, 1:5000; Cell Signaling Technology, Danvers, MA, USA) overnight at 4°C. .. They were then incubated with secondary antibody (IRDye, 1:10,000; LICORbio) for 1 hour at room temperature and imaged using the Odyssey cLX Imager (LICORbio).

Expressing:

Phospho-proteomics:

other:

Article Title: GCGR agonism requires GABAergic signaling in the medial basal hypothalamus to promote weight loss in obese mice
Article Snippet: Quantitative real-time PCR was performed using the QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific) with TaqMan gene expression assays.

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