Overlay Assay:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Incubation:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Blocking Assay:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Membrane:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Imaging:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Extraction:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Positron Emission Tomography:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Cell Culture:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Immunocytochemistry:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Saline:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Transfection:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Next-Generation Sequencing:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Purification:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
Cell Based Assay:Article Title: OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice
Article Snippet: uctions. Procedures described by Deng et al. ( 2016 ) were referenced for the protein-lipid blot overlay assay. Briefly, 25 µg MBP fusion proteins were diluted in 8 mL blocking buffer and incubated with membrane lipid strips (Echelon Biosciences, USA) for 2 h. After incubation, the membrane was probed with a 1:8000 dilution of Anti-MBP monoclonal antibody (TransGen Biotech, China), followed by washing to remove unbound antibody. The membrane was t
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