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Fisher Scientific inserts
Inserts, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inserts/inserts+transwell/pmc13158619-92-15-23
Average 86 stars, based on 1 article reviews
inserts - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Pore Size:

Article Title: METTL16 inhibitors and uses thereof
Article Snippet: .. Transwell assays were carried out using Corning transwell permeable inserts (pore size, 8 μm, 07-200-150, Fisher Scientific) in 24-well plate. ..

Article Title: Plant-derived betaine enhances barrier integrity and immune responses in canine intestinal epithelial cells and macrophages under endotoxin challenge
Article Snippet: The permeability of MCA-B1 cell monolayers was assessed by measuring the transepithelial passage of Fluorescein isothiocyanate–dextran (FITC-Dextran 4KD; Merck Life Science A/S, Søborg, Denmark). .. Briefly, MCA-B1 cells were seeded on transwell inserts (Polyester membrane, 0.33 cm 2 surface area, 0.4 μm pore size, Corning, Fisher scientific, Vantaa, Finland) at a density of 2 × 10 4 cells/well in 100 μL of culture medium, until confluency. ..

Article Title: Antiviral therapeutic compounds and compositions for use in treatment of coronavirus and influenza virus
Article Snippet: .. Caco-2 cells are seeded onto collagen-coated Transwell polycarbonate membrane inserts (12-mm diameter; 1-μm pore size) (Fisher Scientific, Mississauga, ON) at a density of 60,000 cells/cm2. ..

Article Title: Biological responses of human tracheobronchial mucociliary pseudo-epithelium repeatedly exposed at the Air-Liquid Interface to PM 10 indoor particles.
Article Snippet: .. They were then detached and spread at 30,000 cells/150 μL/insert in bronchial epithelial cell growth medium onto Transwell® inserts (6.5 mm; pore size 0.4 μm; Costar, Fisher scientific, France) pre-coated with a collagen solution (30 μg/mL in 0.02 M acetic acid) for 30 min. Growth medium was also added at the basal side (550 μL /well) for 3 days until confluency. .. They were then detached and spread at 30,000 cells/150 μL/insert in bronchial epithelial cell growth medium onto Transwell inserts (6.5 mm; pore size 0.4 μm; Costar, Fisher scientific, France) pre-coated with a collagen solution (30 μg/mL in 0.02 M acetic acid) for 30 min. Growth medium was also added at the basal side (550 μL /well) for 3 days until confluency.

Article Title: Antiviral therapeutic compounds and compositions for use in treatment of coronavirus and influenza virus
Article Snippet: .. Calu-3 (human airway epithelial cell line) host cells were seeded onto collagen-coated Transwell polycarbonate membrane inserts (12-mm diameter; 1-μm pore size) (Fisher Scientific, Winnipeg, MB) at a density of 60,000 cells/cm2. ..

Article Title: Inflammatory microglia signals drive A1-like polarization of astrocytes even in the presence of HIV-1 Tat.
Article Snippet: .. Briefly, astrocytes were seeded on the underside of inverted PET transwell inserts with a pore size of 3.0 μm (0.3 cm2, Falcon, Fisher Scientific, Waltham, MA, USA) at a density of 100,000 cells/well [47–49]. .. This process involved feeding astrocytes every 5 to 45 min for 4 h. Following this basal seeding procedure, the transwell inserts were inverted and placed within a 24-well plate (Falcon, Fisher Scientific, Waltham, MA, USA), and BMECs were seeded on the 0.2% gelatin-coated apical side of the transwell membrane at a density of 40,000 cells/well.

Membrane:

Article Title: Plant-derived betaine enhances barrier integrity and immune responses in canine intestinal epithelial cells and macrophages under endotoxin challenge
Article Snippet: The permeability of MCA-B1 cell monolayers was assessed by measuring the transepithelial passage of Fluorescein isothiocyanate–dextran (FITC-Dextran 4KD; Merck Life Science A/S, Søborg, Denmark). .. Briefly, MCA-B1 cells were seeded on transwell inserts (Polyester membrane, 0.33 cm 2 surface area, 0.4 μm pore size, Corning, Fisher scientific, Vantaa, Finland) at a density of 2 × 10 4 cells/well in 100 μL of culture medium, until confluency. ..

Article Title: Antiviral therapeutic compounds and compositions for use in treatment of coronavirus and influenza virus
Article Snippet: .. Caco-2 cells are seeded onto collagen-coated Transwell polycarbonate membrane inserts (12-mm diameter; 1-μm pore size) (Fisher Scientific, Mississauga, ON) at a density of 60,000 cells/cm2. ..

Article Title: Antiviral therapeutic compounds and compositions for use in treatment of coronavirus and influenza virus
Article Snippet: .. Calu-3 (human airway epithelial cell line) host cells were seeded onto collagen-coated Transwell polycarbonate membrane inserts (12-mm diameter; 1-μm pore size) (Fisher Scientific, Winnipeg, MB) at a density of 60,000 cells/cm2. ..

Article Title: Development of a high-throughput screening platform for identification of functional BACH1 inhibitors reveals compounds with anti-invasive potential
Article Snippet: .. The transwell migration assay was conducted using CorningTM TranswellTM Multiple Well Plate with 6.5 mm inserts and 8.0 μm pore permeable polyester membrane (Fisher Scientific). ..

Article Title: Inflammatory microglia signals drive A1-like polarization of astrocytes even in the presence of HIV-1 Tat.
Article Snippet: Briefly, astrocytes were seeded on the underside of inverted PET transwell inserts with a pore size of 3.0 μm (0.3 cm2, Falcon, Fisher Scientific, Waltham, MA, USA) at a density of 100,000 cells/well [47–49]. .. This process involved feeding astrocytes every 5 to 45 min for 4 h. Following this basal seeding procedure, the transwell inserts were inverted and placed within a 24-well plate (Falcon, Fisher Scientific, Waltham, MA, USA), and BMECs were seeded on the 0.2% gelatin-coated apical side of the transwell membrane at a density of 40,000 cells/well. ..

Transwell Migration Assay:

Article Title: Development of a high-throughput screening platform for identification of functional BACH1 inhibitors reveals compounds with anti-invasive potential
Article Snippet: .. The transwell migration assay was conducted using CorningTM TranswellTM Multiple Well Plate with 6.5 mm inserts and 8.0 μm pore permeable polyester membrane (Fisher Scientific). ..

Positron Emission Tomography:

Article Title: Inflammatory microglia signals drive A1-like polarization of astrocytes even in the presence of HIV-1 Tat.
Article Snippet: .. Briefly, astrocytes were seeded on the underside of inverted PET transwell inserts with a pore size of 3.0 μm (0.3 cm2, Falcon, Fisher Scientific, Waltham, MA, USA) at a density of 100,000 cells/well [47–49]. .. This process involved feeding astrocytes every 5 to 45 min for 4 h. Following this basal seeding procedure, the transwell inserts were inverted and placed within a 24-well plate (Falcon, Fisher Scientific, Waltham, MA, USA), and BMECs were seeded on the 0.2% gelatin-coated apical side of the transwell membrane at a density of 40,000 cells/well.



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(A) Sample and plate preparation steps for mounting before performing the 3D-4i multiplexing protocol. (B) Visualization of the zebrafish embryos mounted inside the imaging plate well. Example fluorescence image in side view (x–z) acquired with a spinning disk confocal microscope and 1 μm z-step size (arrow depicts imaging direction from objective). Scale bar: 50 μm. (C) Example overview of <t>a</t> <t>96-well</t> imaging plate with 5–6 zebrafish embryos mounted each in 24 individual wells. DAPI signal with color corresponding to cycle 1 (red) and 4 (blue) of the 3D-4i multiplexing protocol. Total number of embryos is 138; the number of detached embryos during a four-cycle 3D-4i protocol is 1 (second column from the left, third row from the top).
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Image Search Results


(A) Sample and plate preparation steps for mounting before performing the 3D-4i multiplexing protocol. (B) Visualization of the zebrafish embryos mounted inside the imaging plate well. Example fluorescence image in side view (x–z) acquired with a spinning disk confocal microscope and 1 μm z-step size (arrow depicts imaging direction from objective). Scale bar: 50 μm. (C) Example overview of a 96-well imaging plate with 5–6 zebrafish embryos mounted each in 24 individual wells. DAPI signal with color corresponding to cycle 1 (red) and 4 (blue) of the 3D-4i multiplexing protocol. Total number of embryos is 138; the number of detached embryos during a four-cycle 3D-4i protocol is 1 (second column from the left, third row from the top).

Journal: Bio-protocol

Article Title: 3D Iterative Immunofluorescence Imaging on Whole-Mount Samples

doi: 10.21769/BioProtoc.5838

Figure Lengend Snippet: (A) Sample and plate preparation steps for mounting before performing the 3D-4i multiplexing protocol. (B) Visualization of the zebrafish embryos mounted inside the imaging plate well. Example fluorescence image in side view (x–z) acquired with a spinning disk confocal microscope and 1 μm z-step size (arrow depicts imaging direction from objective). Scale bar: 50 μm. (C) Example overview of a 96-well imaging plate with 5–6 zebrafish embryos mounted each in 24 individual wells. DAPI signal with color corresponding to cycle 1 (red) and 4 (blue) of the 3D-4i multiplexing protocol. Total number of embryos is 138; the number of detached embryos during a four-cycle 3D-4i protocol is 1 (second column from the left, third row from the top).

Article Snippet: Laboratory centrifuge with rotors compatible with 96-well plate inserts (e.g., Eppendorf, model: 5810R) 8.

Techniques: Multiplexing, Imaging, Fluorescence, Spinning Disk, Confocal, Microscopy