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permeable inserts  (CELLTREAT Scientific)


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    Structured Review

    CELLTREAT Scientific permeable inserts
    Permeable Inserts, supplied by CELLTREAT Scientific, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inserts/Permeable+Cell+Culture+Inserts/pmc13120886-22-11-18
    Average 94 stars, based on 6 article reviews
    permeable inserts - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Sterility:

    Article Title: Precision-cut lung slices as an ex vivo model to study Pneumocystis murina survival and antimicrobial susceptibility
    Article Snippet: .. The freshly cut slices were placed into sterile submerged well (CELLTREAT, 229111) or transwell (CELLTREAT, 230621) tissue culture plates with 1× DMEM containing 1× P/S and incubated in 5% CO 2 at 37°C overnight to allow recovery from slicing. .. The viability of the lung slices was tested using PrestoBlue cell viability reagent (ThermoFisher Scientific, A13261) as outlined below.

    Article Title: Circular RNA vaccines with long-term lymph node-targeting delivery stability after lyophilization induce potent and persistent immune responses.
    Article Snippet: .. The freshly cut slices were placed into sterile submerged well (CELLTREAT, 229111) or transwell (CELLTREAT, 230621) tissue culture plates with 1× DMEM containing 1× P/S and incubated in 5% CO2 at 37°C overnight to allow recovery from slicing. .. The viability of the lung slices was tested using PrestoBlue cell viability reagent (ThermoFisher Scientific, A13261) as outlined below.

    Incubation:

    Article Title: Precision-cut lung slices as an ex vivo model to study Pneumocystis murina survival and antimicrobial susceptibility
    Article Snippet: .. The freshly cut slices were placed into sterile submerged well (CELLTREAT, 229111) or transwell (CELLTREAT, 230621) tissue culture plates with 1× DMEM containing 1× P/S and incubated in 5% CO 2 at 37°C overnight to allow recovery from slicing. .. The viability of the lung slices was tested using PrestoBlue cell viability reagent (ThermoFisher Scientific, A13261) as outlined below.

    Article Title: Circular RNA vaccines with long-term lymph node-targeting delivery stability after lyophilization induce potent and persistent immune responses.
    Article Snippet: .. The freshly cut slices were placed into sterile submerged well (CELLTREAT, 229111) or transwell (CELLTREAT, 230621) tissue culture plates with 1× DMEM containing 1× P/S and incubated in 5% CO2 at 37°C overnight to allow recovery from slicing. .. The viability of the lung slices was tested using PrestoBlue cell viability reagent (ThermoFisher Scientific, A13261) as outlined below.

    Article Title: Circular RNA vaccines with long-term lymph node-targeting delivery stability after lyophilization induce potent and persistent immune responses.
    Article Snippet: .. Following incubation at 37°C, 5% CO2 overnight, PCLS were moved to new 12-well tissue culture plate (CELLTREAT, 229112) with 450 μL fresh 1× DMEM containing 1× P/S, or permeable cell culture inserts, pore size 4 μm, packed in 12-well plate (CELLTREAT, 230621) with 450 μL fresh 1× DMEM containing 1× P/S. ..

    Article Title: Precision-cut lung slices as an ex vivo model to study Pneumocystis murina survival and antimicrobial susceptibility
    Article Snippet: .. Following incubation at 37°C, 5% CO2 overnight, PCLS were moved to new 12-well tissue culture plate (CELLTREAT, 229112) with 450 μL fresh 1× DMEM containing 1× P/S, or permeable cell culture inserts, pore size 4 μm, packed in 12-well plate (CELLTREAT, 230621) with 450 μL fresh 1× DMEM containing 1× P/S. ..

    Membrane:

    Article Title: Influence of Insert Brand and Culture Method on Ciliary Activity and Epithelial Cell Types in Human Nasal Air–Liquid Interface Cell Cultures
    Article Snippet: .. Briefly, after obtaining hNECs by nasal brushings from the inferior nasal turbinate area of both nostrils, the cells were proliferated over two passages in expansion media (PneumaCult-Expansion-Plus complete medium, Stemcell Technologies, Vancouver, BC, Canada, #05040) and 200’000 cells were seeded onto the apical sides of three uncoated insert types (Corning ® Transwell ® polyester membrane inserts, #3460, Sigma-Aldrich, Merck & Cie, Buchs, Switzerland; CELLTREAT ® Permeable Cell Culture Inserts #230621; ThinCert ® Cell Culture Inserts, #665641, greiner bio-one; for details of the composition, see ) placed in 12-well cell culture plates (Falcon 12-well plate, #353043). ..

    Cell Culture:

    Article Title: Influence of Insert Brand and Culture Method on Ciliary Activity and Epithelial Cell Types in Human Nasal Air–Liquid Interface Cell Cultures
    Article Snippet: .. Briefly, after obtaining hNECs by nasal brushings from the inferior nasal turbinate area of both nostrils, the cells were proliferated over two passages in expansion media (PneumaCult-Expansion-Plus complete medium, Stemcell Technologies, Vancouver, BC, Canada, #05040) and 200’000 cells were seeded onto the apical sides of three uncoated insert types (Corning ® Transwell ® polyester membrane inserts, #3460, Sigma-Aldrich, Merck & Cie, Buchs, Switzerland; CELLTREAT ® Permeable Cell Culture Inserts #230621; ThinCert ® Cell Culture Inserts, #665641, greiner bio-one; for details of the composition, see ) placed in 12-well cell culture plates (Falcon 12-well plate, #353043). ..

    Article Title: Circular RNA vaccines with long-term lymph node-targeting delivery stability after lyophilization induce potent and persistent immune responses.
    Article Snippet: .. Following incubation at 37°C, 5% CO2 overnight, PCLS were moved to new 12-well tissue culture plate (CELLTREAT, 229112) with 450 μL fresh 1× DMEM containing 1× P/S, or permeable cell culture inserts, pore size 4 μm, packed in 12-well plate (CELLTREAT, 230621) with 450 μL fresh 1× DMEM containing 1× P/S. ..

    Article Title: Precision-cut lung slices as an ex vivo model to study Pneumocystis murina survival and antimicrobial susceptibility
    Article Snippet: .. Following incubation at 37°C, 5% CO2 overnight, PCLS were moved to new 12-well tissue culture plate (CELLTREAT, 229112) with 450 μL fresh 1× DMEM containing 1× P/S, or permeable cell culture inserts, pore size 4 μm, packed in 12-well plate (CELLTREAT, 230621) with 450 μL fresh 1× DMEM containing 1× P/S. ..

    Pore Size:

    Article Title: Circular RNA vaccines with long-term lymph node-targeting delivery stability after lyophilization induce potent and persistent immune responses.
    Article Snippet: .. Following incubation at 37°C, 5% CO2 overnight, PCLS were moved to new 12-well tissue culture plate (CELLTREAT, 229112) with 450 μL fresh 1× DMEM containing 1× P/S, or permeable cell culture inserts, pore size 4 μm, packed in 12-well plate (CELLTREAT, 230621) with 450 μL fresh 1× DMEM containing 1× P/S. ..

    Article Title: Precision-cut lung slices as an ex vivo model to study Pneumocystis murina survival and antimicrobial susceptibility
    Article Snippet: .. Following incubation at 37°C, 5% CO2 overnight, PCLS were moved to new 12-well tissue culture plate (CELLTREAT, 229112) with 450 μL fresh 1× DMEM containing 1× P/S, or permeable cell culture inserts, pore size 4 μm, packed in 12-well plate (CELLTREAT, 230621) with 450 μL fresh 1× DMEM containing 1× P/S. ..



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    Image Search Results


    Evaluation of angiogenic potential induced by Mg 2+ and NRP-1. ( A ) Scratch assay in three different microenvironments. ( B ) Transwell assay in three different microenvironments. ( C ) Quantitative analysis of wound healing area. ( D ) Quantitative analysis of number of migration cells. ( E ) Immunofluorescence staining of VEGFA and FGF2, with DAPI for nuclear staining and F-actin for cytoskeleton labeling. ( F ) Tube formation evaluation in four different microenvironments. ( G ) Quantitative analysis of number of junction. ( H ) Quantitative analysis of Flu intensity. ( I ) Western-blot analysis of VEGFA, FGF2, and Nr4a1. ( J-L ) Quantitative analysis of relative protein expression of VEGFA, FGF2, and Nr4a1. Data are presented as mean values ± s.d. (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).

    Journal: Bioactive Materials

    Article Title: Reconstructing the ischemic osteogenic microenvironment through hierarchical scaffolds orchestrating Mg 2+ signaling and neuropilin-1–mediated angiogenesis

    doi: 10.1016/j.bioactmat.2026.02.031

    Figure Lengend Snippet: Evaluation of angiogenic potential induced by Mg 2+ and NRP-1. ( A ) Scratch assay in three different microenvironments. ( B ) Transwell assay in three different microenvironments. ( C ) Quantitative analysis of wound healing area. ( D ) Quantitative analysis of number of migration cells. ( E ) Immunofluorescence staining of VEGFA and FGF2, with DAPI for nuclear staining and F-actin for cytoskeleton labeling. ( F ) Tube formation evaluation in four different microenvironments. ( G ) Quantitative analysis of number of junction. ( H ) Quantitative analysis of Flu intensity. ( I ) Western-blot analysis of VEGFA, FGF2, and Nr4a1. ( J-L ) Quantitative analysis of relative protein expression of VEGFA, FGF2, and Nr4a1. Data are presented as mean values ± s.d. (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).

    Article Snippet: For the Transwell assay, BMSCs were seeded in the upper chambers of Transwell inserts (8.0 μm pore size; Servicebio, China), while different culture conditions were applied in the lower chambers according to the experimental groups.

    Techniques: Wound Healing Assay, Transwell Assay, Migration, Immunofluorescence, Staining, Labeling, Western Blot, Expressing