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Structured Review

Servicebio Inc transwell inserts
Evaluation of angiogenic potential induced by Mg 2+ and NRP-1. ( A ) Scratch assay in three different microenvironments. ( B ) <t>Transwell</t> assay in three different microenvironments. ( C ) Quantitative analysis of wound healing area. ( D ) Quantitative analysis of number of migration cells. ( E ) Immunofluorescence staining of VEGFA and FGF2, with DAPI for nuclear staining and F-actin for cytoskeleton labeling. ( F ) Tube formation evaluation in four different microenvironments. ( G ) Quantitative analysis of number of junction. ( H ) Quantitative analysis of Flu intensity. ( I ) Western-blot analysis of VEGFA, FGF2, and Nr4a1. ( J-L ) Quantitative analysis of relative protein expression of VEGFA, FGF2, and Nr4a1. Data are presented as mean values ± s.d. (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).
Transwell Inserts, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Reconstructing the ischemic osteogenic microenvironment through hierarchical scaffolds orchestrating Mg 2+ signaling and neuropilin-1–mediated angiogenesis"

Article Title: Reconstructing the ischemic osteogenic microenvironment through hierarchical scaffolds orchestrating Mg 2+ signaling and neuropilin-1–mediated angiogenesis

Journal: Bioactive Materials

doi: 10.1016/j.bioactmat.2026.02.031

Evaluation of angiogenic potential induced by Mg 2+ and NRP-1. ( A ) Scratch assay in three different microenvironments. ( B ) Transwell assay in three different microenvironments. ( C ) Quantitative analysis of wound healing area. ( D ) Quantitative analysis of number of migration cells. ( E ) Immunofluorescence staining of VEGFA and FGF2, with DAPI for nuclear staining and F-actin for cytoskeleton labeling. ( F ) Tube formation evaluation in four different microenvironments. ( G ) Quantitative analysis of number of junction. ( H ) Quantitative analysis of Flu intensity. ( I ) Western-blot analysis of VEGFA, FGF2, and Nr4a1. ( J-L ) Quantitative analysis of relative protein expression of VEGFA, FGF2, and Nr4a1. Data are presented as mean values ± s.d. (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).
Figure Legend Snippet: Evaluation of angiogenic potential induced by Mg 2+ and NRP-1. ( A ) Scratch assay in three different microenvironments. ( B ) Transwell assay in three different microenvironments. ( C ) Quantitative analysis of wound healing area. ( D ) Quantitative analysis of number of migration cells. ( E ) Immunofluorescence staining of VEGFA and FGF2, with DAPI for nuclear staining and F-actin for cytoskeleton labeling. ( F ) Tube formation evaluation in four different microenvironments. ( G ) Quantitative analysis of number of junction. ( H ) Quantitative analysis of Flu intensity. ( I ) Western-blot analysis of VEGFA, FGF2, and Nr4a1. ( J-L ) Quantitative analysis of relative protein expression of VEGFA, FGF2, and Nr4a1. Data are presented as mean values ± s.d. (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).

Techniques Used: Wound Healing Assay, Transwell Assay, Migration, Immunofluorescence, Staining, Labeling, Western Blot, Expressing

Related Articles

Transfection:

Article Title: PDK1 downregulation impairs decidualization via dysregulated glycolysis: Potential relevance to preeclampsia.
Article Snippet: Preeclampsia (PE) is a pregnancy-specific syndrome affecting over 8 million mother-infant pairs annually; while most prior studies on PE pathogenesis have focused on the placenta, decidualization is fundamental to placentation and subsequent pregnancy progression.. Pyruvate dehydrogenase kinase-1 (PDK1) is a key regulator of metabolic switching, as it promotes glycolysis and inhibits oxidative phosphorylation.. Although PDK1 is aberrantly upregulated in various tumors, its role in PE remains unclear.

Suspension:

Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells
Article Snippet: .. A volume of 100 μL of this cell suspension was carefully introduced into the upper chamber of the Transwell insert (Servicebio, Cat. No. C6610), and 600 μL of medium supplemented with 10% fetal bovine serum was added to each well of the lower chamber. ..

Migration:

Article Title: Transmembrane protein 9 as a novel biomarker promotes oral squamous cell carcinoma growth via IL1RN and serves as an immune therapeutic target
Article Snippet: Statistical analyses were conducted using GraphPad Prism v6.0. .. Cell migration was assessed utilizing 8μm pore Transwell chambers (Servicebio, Wuhan, China, Cat# WG3415) devoid of Matrigel coating. ..

Article Title: Robust transcriptomic hallmarks targeting intratumor heterogeneity in intrahepatic cholangiocarcinoma
Article Snippet: Proteins were separated by Tricine-PAGE (Epizyme Biomedical Technology, PG310) and transferred to polyvinylidene fluoride (PVDF) membranes (Epizyme Biomedical Technology, WJ001). .. For migration assay, 3 × 10 5 neutrophils were seeded in the upper chamber of 3-μm pore Transwell inserts (Servicebio, WG3415). .. The following primary antibodies were used: anti-CXCL5 (R&D Systems, AF254, 1:1000), anti-CXCL5 (Abmart, PH9257, 1:1000), and anti-GAPDH (Servicebio, GB11002, 1:1000).

other:

Article Title: Reconstructing the ischemic osteogenic microenvironment through hierarchical scaffolds orchestrating Mg2+ signaling and neuropilin-1–mediated angiogenesis
Article Snippet: Images were captured at 0, 24, and 48 h using an inverted microscope (Zeiss, Germany), and the wound closure area was quantified using ImageJ.

Article Title: Robust transcriptomic hallmarks targeting intratumor heterogeneity in intrahepatic cholangiocarcinoma.
Article Snippet: A CXCL5-expressing lentiviral vector was constructed by Genomeditech, and the murine iCCA cell line KTP with stable CXCL5 overexpression was generated via lentiviral infection.

Transwell Assay:

Article Title: Reconstructing the ischemic osteogenic microenvironment through hierarchical scaffolds orchestrating Mg 2+ signaling and neuropilin-1–mediated angiogenesis
Article Snippet: Images were captured at 0, 24, and 48 h using an inverted microscope (Zeiss, Germany), and the wound closure area was quantified using ImageJ. .. For the Transwell assay, BMSCs were seeded in the upper chambers of Transwell inserts (8.0 μm pore size; Servicebio, China), while different culture conditions were applied in the lower chambers according to the experimental groups. ..

Pore Size:

Article Title: Reconstructing the ischemic osteogenic microenvironment through hierarchical scaffolds orchestrating Mg 2+ signaling and neuropilin-1–mediated angiogenesis
Article Snippet: Images were captured at 0, 24, and 48 h using an inverted microscope (Zeiss, Germany), and the wound closure area was quantified using ImageJ. .. For the Transwell assay, BMSCs were seeded in the upper chambers of Transwell inserts (8.0 μm pore size; Servicebio, China), while different culture conditions were applied in the lower chambers according to the experimental groups. ..



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Image Search Results


(A) Sample and plate preparation steps for mounting before performing the 3D-4i multiplexing protocol. (B) Visualization of the zebrafish embryos mounted inside the imaging plate well. Example fluorescence image in side view (x–z) acquired with a spinning disk confocal microscope and 1 μm z-step size (arrow depicts imaging direction from objective). Scale bar: 50 μm. (C) Example overview of a 96-well imaging plate with 5–6 zebrafish embryos mounted each in 24 individual wells. DAPI signal with color corresponding to cycle 1 (red) and 4 (blue) of the 3D-4i multiplexing protocol. Total number of embryos is 138; the number of detached embryos during a four-cycle 3D-4i protocol is 1 (second column from the left, third row from the top).

Journal: Bio-protocol

Article Title: 3D Iterative Immunofluorescence Imaging on Whole-Mount Samples

doi: 10.21769/BioProtoc.5838

Figure Lengend Snippet: (A) Sample and plate preparation steps for mounting before performing the 3D-4i multiplexing protocol. (B) Visualization of the zebrafish embryos mounted inside the imaging plate well. Example fluorescence image in side view (x–z) acquired with a spinning disk confocal microscope and 1 μm z-step size (arrow depicts imaging direction from objective). Scale bar: 50 μm. (C) Example overview of a 96-well imaging plate with 5–6 zebrafish embryos mounted each in 24 individual wells. DAPI signal with color corresponding to cycle 1 (red) and 4 (blue) of the 3D-4i multiplexing protocol. Total number of embryos is 138; the number of detached embryos during a four-cycle 3D-4i protocol is 1 (second column from the left, third row from the top).

Article Snippet: Laboratory centrifuge with rotors compatible with 96-well plate inserts (e.g., Eppendorf, model: 5810R) 8.

Techniques: Multiplexing, Imaging, Fluorescence, Spinning Disk, Confocal, Microscopy