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amplification  (New England Biolabs)


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    Structured Review

    New England Biolabs amplification
    Amplification, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/input+cdna/NEBNext+Single+Cell%2FLow+Input+cDNA+Synt/pmc12962854-237-32-33
    Average 97 stars, based on 383 article reviews
    amplification - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Sequencing:

    Article Title: Global genomic diversity of the selfing nematode Caenorhabditis tropicalis correlates with geography
    Article Snippet: .. Library preparation was performed at the Duke Center for Genomic and Computational Biology’s Sequencing and Genomic Technologies Core Facility using the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module (New England Biolabs, catalog no. E6421) and the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, catalog no. 100-938-900). ..

    cDNA Synthesis:

    Article Title: Global genomic diversity of the selfing nematode Caenorhabditis tropicalis correlates with geography
    Article Snippet: .. Library preparation was performed at the Duke Center for Genomic and Computational Biology’s Sequencing and Genomic Technologies Core Facility using the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module (New England Biolabs, catalog no. E6421) and the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, catalog no. 100-938-900). ..

    Article Title: miR-203 facilitates timely cell fate transitions via epigenetic modulation during early embryogenesis
    Article Snippet: Conversion of BCL (base calls) binary files to FASTQ format is subsequently performed with bcl2fastq2 (Illumina). .. For ultralow-input RNA-seq, 15 to 20 embryos were collected per sample and resuspended in 8 ml of low-input lysis buffer of the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification module (#E6421S). .. Whole cells were processed with the “NEBNext Single Cell/Low Input RNA Library Prep” kit [New England Biolabs (NEB), #E6420] by following the manufacturer’s instructions.

    Article Title: The Zn(II)2Cys6 transcription factor Ds Ace3 is a major activator of cellulases in the white-rot fungus Dichomitus squalens
    Article Snippet: .. Full-length cDNA synthesis was performed using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module kit. .. First-strand cDNA was amplified using NEBNext High-Fidelity 2× PCR Master Mix with barcoded cDNA PCR primers and 11–14 cycles of amplification.

    Article Title: Protocol for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cells
    Article Snippet: Hyperprep kit (KAPA Biosystems) , Roche , Cat# 7962347001. .. NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module , New England Biolabs , Cat#: E6421S for 24 reactions or E6421L for 96 reactions. .. Bioanalyzer High Sensitivity kit , Agilent , NA.

    Article Title: Whole genomes reveal subpopulations and isolation-by-distance patterns in the Norwegian lemming.
    Article Snippet: .. From a total of 300 ng of RNA one PacBio SMRTbellTM library was prepared as AR TIC LE IN PR ES S described in "Procedure & Checklist – Iso-SeqTM Express Template Preparation for Sequel® and Sequel II Systems" (PN 101-763-800 Version 02 (October 2019)) using the NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module, the Iso-Seq Express Oligo Kit, ProNex beads (Promega) and the SMRTbell Express Template Prep Kit 2.0. ..

    Article Title: Structure and sequence evolution in the pennycress (Thlaspi arvense) pangenome.
    Article Snippet: .. We synthesized full-length cDNA with NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module kit (catalog no.: E6421) by amplifying first-strand cDNA with NEBNext High-Fidelity 29 PCRMasterMix (catalog no.:M0541) with barcoded cDNAPCR primers for 11–14 cycles. .. We then purified cDNA using 19 AMPure PB beads (catalog no.: 100–265-900; PacBio) for nonsize selection or BluePippin (Sage Science, Beverly, MA, USA) for 2–10-kb size selection.

    Amplification:

    Article Title: Global genomic diversity of the selfing nematode Caenorhabditis tropicalis correlates with geography
    Article Snippet: .. Library preparation was performed at the Duke Center for Genomic and Computational Biology’s Sequencing and Genomic Technologies Core Facility using the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module (New England Biolabs, catalog no. E6421) and the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, catalog no. 100-938-900). ..

    Article Title: miR-203 facilitates timely cell fate transitions via epigenetic modulation during early embryogenesis
    Article Snippet: Conversion of BCL (base calls) binary files to FASTQ format is subsequently performed with bcl2fastq2 (Illumina). .. For ultralow-input RNA-seq, 15 to 20 embryos were collected per sample and resuspended in 8 ml of low-input lysis buffer of the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification module (#E6421S). .. Whole cells were processed with the “NEBNext Single Cell/Low Input RNA Library Prep” kit [New England Biolabs (NEB), #E6420] by following the manufacturer’s instructions.

    Article Title: The Zn(II)2Cys6 transcription factor Ds Ace3 is a major activator of cellulases in the white-rot fungus Dichomitus squalens
    Article Snippet: .. Full-length cDNA synthesis was performed using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module kit. .. First-strand cDNA was amplified using NEBNext High-Fidelity 2× PCR Master Mix with barcoded cDNA PCR primers and 11–14 cycles of amplification.

    Article Title: Protocol for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cells
    Article Snippet: Hyperprep kit (KAPA Biosystems) , Roche , Cat# 7962347001. .. NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module , New England Biolabs , Cat#: E6421S for 24 reactions or E6421L for 96 reactions. .. Bioanalyzer High Sensitivity kit , Agilent , NA.

    Article Title: Whole genomes reveal subpopulations and isolation-by-distance patterns in the Norwegian lemming.
    Article Snippet: .. From a total of 300 ng of RNA one PacBio SMRTbellTM library was prepared as AR TIC LE IN PR ES S described in "Procedure & Checklist – Iso-SeqTM Express Template Preparation for Sequel® and Sequel II Systems" (PN 101-763-800 Version 02 (October 2019)) using the NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module, the Iso-Seq Express Oligo Kit, ProNex beads (Promega) and the SMRTbell Express Template Prep Kit 2.0. ..

    Article Title: Structure and sequence evolution in the pennycress (Thlaspi arvense) pangenome.
    Article Snippet: .. We synthesized full-length cDNA with NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module kit (catalog no.: E6421) by amplifying first-strand cDNA with NEBNext High-Fidelity 29 PCRMasterMix (catalog no.:M0541) with barcoded cDNAPCR primers for 11–14 cycles. .. We then purified cDNA using 19 AMPure PB beads (catalog no.: 100–265-900; PacBio) for nonsize selection or BluePippin (Sage Science, Beverly, MA, USA) for 2–10-kb size selection.

    RNA Sequencing:

    Article Title: miR-203 facilitates timely cell fate transitions via epigenetic modulation during early embryogenesis
    Article Snippet: Conversion of BCL (base calls) binary files to FASTQ format is subsequently performed with bcl2fastq2 (Illumina). .. For ultralow-input RNA-seq, 15 to 20 embryos were collected per sample and resuspended in 8 ml of low-input lysis buffer of the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification module (#E6421S). .. Whole cells were processed with the “NEBNext Single Cell/Low Input RNA Library Prep” kit [New England Biolabs (NEB), #E6420] by following the manufacturer’s instructions.

    Lysis:

    Article Title: miR-203 facilitates timely cell fate transitions via epigenetic modulation during early embryogenesis
    Article Snippet: Conversion of BCL (base calls) binary files to FASTQ format is subsequently performed with bcl2fastq2 (Illumina). .. For ultralow-input RNA-seq, 15 to 20 embryos were collected per sample and resuspended in 8 ml of low-input lysis buffer of the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification module (#E6421S). .. Whole cells were processed with the “NEBNext Single Cell/Low Input RNA Library Prep” kit [New England Biolabs (NEB), #E6420] by following the manufacturer’s instructions.

    other:

    Article Title: Evolution of Highly Repetitive Silk Genes in the Luna Moth, Actias luna
    Article Snippet: Total RNA from each larval instar silk gland was diluted to ∼100 ng, after which full-length cDNA was synthesized with amplification as described in the protocol for Low Input RNA:cDNA Synthesis and Amplification (New England Biolabs, Cat #E6421).

    Synthesized:

    Article Title: Structure and sequence evolution in the pennycress (Thlaspi arvense) pangenome.
    Article Snippet: .. We synthesized full-length cDNA with NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module kit (catalog no.: E6421) by amplifying first-strand cDNA with NEBNext High-Fidelity 29 PCRMasterMix (catalog no.:M0541) with barcoded cDNAPCR primers for 11–14 cycles. .. We then purified cDNA using 19 AMPure PB beads (catalog no.: 100–265-900; PacBio) for nonsize selection or BluePippin (Sage Science, Beverly, MA, USA) for 2–10-kb size selection.



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