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Narishige inc narishige injection system
Narishige Injection System, supplied by Narishige inc, used in various techniques. Bioz Stars score: 97/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/injection/Injection+System/pm41504023-94-39-43
Average 97 stars, based on 39 article reviews
narishige injection system - by Bioz Stars, 2026-11
97/100 stars

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Related Articles

Injection:

Article Title: Synergistic insecticidal mechanism of 2-undecanone and decanal against Tribolium castaneum larvae via dual suppression of GST and FDa pathways
Article Snippet: Tribolium castaneum (Herbst) is a major stored-product pest, necessitating safer alternatives targeting its destructive larval stage.. This study investigated the synergistic insecticidal effects of 2-undecanone and decanal, two active constituents from Houttuynia cordata essential oil, against T. castaneum larvae and elucidated the underlying mechanisms via quantitative real-time PCR (qRT-PCR), RNA interference (RNAi), histopathology, molecular docking, ELISA, and enzyme activity assays.. Contact toxicity assays identified the 5:5 mixture as optimally effective, exhibiting significant synergy (R ≥ 1.5) and the lowest LD50 (14.8 μg/larva).

Article Title: A positive feedback loop between germ cells and gonads induces and maintains cnidarian sexual reproduction
Article Snippet: Sexual polyps were incubated for one hour in 150 mM BrdU (Sigma; Cat. #B5002) in ASW and fixed in 4% paraformaldedhyde/ASW. .. One-cell stage embryos of wild type or Tfap2 reporter animal were injected using a Narishige IM 300 microinjection system as described . .. Gls coding sequence in-frame with T2A peptide was synthetized by IDT.

Article Title: Genomic landscape and genetic manipulation of an ectoparasitoid wasp, Gregopimpla kuwanae.
Article Snippet: .. RNAi injections were performed using the Narishige IM-400 microinjection system with the following parameters: injection pressure = 200 hPa; injection time = 0.3 s. RNAi efficiency was assessed by qRT-PCR using a pool of three individuals on the 2nd, 4th, 6th, 8th, and 10th days after dsRNA injection. ..

Article Title: Serotonin-mediated wing dimorphism in Nilaparvata lugens: A survival strategy triggered by host plant senescence.
Article Snippet: The Nanodrop 2000 was used to detect the concentration and purity of dsRNA with a 260/280 ratio of 1.8–2.0 and a total amount of dsRNA ≥ 50 μg. .. The specific primers used in this experimental procedure, including primers for the control gene green fluorescent protein (GFP), are enumerated in Table S1. dsRNA was injected into the thorax of CO2anesthetized 4th-instar nymphs using a Nikon microscope and a Narishige injection system (MN-151, Narishige). ..

Article Title: A positive feedback loop between germ cells and gonads induces and maintains sexual reproduction in a cnidarian
Article Snippet: Sexual polyps were incubated for 1 hour in 150 μM BrdU (Sigma-Aldrich; catalog no. B5002) in ASW and fixed in 4% paraformaldedhyde/ASW. .. One-cell stage embryos of wild-type or Tfap2 reporter animal were injected using a Narishige IM 300 microinjection system as described ( ). .. Gls coding sequence in-frame with T2A peptide was synthetized by IDT.

Article Title: Randomly incorporated genomic N6-methyldeoxyadenosine delays zygotic transcription initiation in a cnidarian.
Article Snippet: .. Cells were injected, prior to first cleavage, using a Narishige IM 300 microinjection system. ..

Article Title: Azadirachtin Inhibits Nuclear Receptor HR3 in the Prothoracic Gland to Block Larval Ecdysis in the Fall Armyworm, Spodoptera frugiperda .
Article Snippet: Azadirachtin has been used to control agricultural pests for a long time; however, the molecular mechanism of azadirachtin on lepidopterans is still not clear.. In this study, the fourth instar larvae of fall armyworm were fed with azadirachtin, and then the ecdysis was blocked in the fourth instar larval stage (L4).. The prothoracic glands (PGs) of the treated larvae were dissected for RNA sequencing to determine the effect of azadirachtin on ecdysis inhibition.

Inverted Microscopy:

Article Title: Synergistic insecticidal mechanism of 2-undecanone and decanal against Tribolium castaneum larvae via dual suppression of GST and FDa pathways
Article Snippet: Tribolium castaneum (Herbst) is a major stored-product pest, necessitating safer alternatives targeting its destructive larval stage.. This study investigated the synergistic insecticidal effects of 2-undecanone and decanal, two active constituents from Houttuynia cordata essential oil, against T. castaneum larvae and elucidated the underlying mechanisms via quantitative real-time PCR (qRT-PCR), RNA interference (RNAi), histopathology, molecular docking, ELISA, and enzyme activity assays.. Contact toxicity assays identified the 5:5 mixture as optimally effective, exhibiting significant synergy (R ≥ 1.5) and the lowest LD50 (14.8 μg/larva).

Microinjection:

Article Title: A positive feedback loop between germ cells and gonads induces and maintains cnidarian sexual reproduction
Article Snippet: Sexual polyps were incubated for one hour in 150 mM BrdU (Sigma; Cat. #B5002) in ASW and fixed in 4% paraformaldedhyde/ASW. .. One-cell stage embryos of wild type or Tfap2 reporter animal were injected using a Narishige IM 300 microinjection system as described . .. Gls coding sequence in-frame with T2A peptide was synthetized by IDT.

Article Title: Genomic landscape and genetic manipulation of an ectoparasitoid wasp, Gregopimpla kuwanae.
Article Snippet: .. RNAi injections were performed using the Narishige IM-400 microinjection system with the following parameters: injection pressure = 200 hPa; injection time = 0.3 s. RNAi efficiency was assessed by qRT-PCR using a pool of three individuals on the 2nd, 4th, 6th, 8th, and 10th days after dsRNA injection. ..

Article Title: A positive feedback loop between germ cells and gonads induces and maintains sexual reproduction in a cnidarian
Article Snippet: Sexual polyps were incubated for 1 hour in 150 μM BrdU (Sigma-Aldrich; catalog no. B5002) in ASW and fixed in 4% paraformaldedhyde/ASW. .. One-cell stage embryos of wild-type or Tfap2 reporter animal were injected using a Narishige IM 300 microinjection system as described ( ). .. Gls coding sequence in-frame with T2A peptide was synthetized by IDT.

Article Title: Randomly incorporated genomic N6-methyldeoxyadenosine delays zygotic transcription initiation in a cnidarian.
Article Snippet: .. Cells were injected, prior to first cleavage, using a Narishige IM 300 microinjection system. ..

Article Title: Azadirachtin Inhibits Nuclear Receptor HR3 in the Prothoracic Gland to Block Larval Ecdysis in the Fall Armyworm, Spodoptera frugiperda .
Article Snippet: Azadirachtin has been used to control agricultural pests for a long time; however, the molecular mechanism of azadirachtin on lepidopterans is still not clear.. In this study, the fourth instar larvae of fall armyworm were fed with azadirachtin, and then the ecdysis was blocked in the fourth instar larval stage (L4).. The prothoracic glands (PGs) of the treated larvae were dissected for RNA sequencing to determine the effect of azadirachtin on ecdysis inhibition.

Quantitative RT-PCR:

Article Title: Genomic landscape and genetic manipulation of an ectoparasitoid wasp, Gregopimpla kuwanae.
Article Snippet: .. RNAi injections were performed using the Narishige IM-400 microinjection system with the following parameters: injection pressure = 200 hPa; injection time = 0.3 s. RNAi efficiency was assessed by qRT-PCR using a pool of three individuals on the 2nd, 4th, 6th, 8th, and 10th days after dsRNA injection. ..

Control:

Article Title: Serotonin-mediated wing dimorphism in Nilaparvata lugens: A survival strategy triggered by host plant senescence.
Article Snippet: The Nanodrop 2000 was used to detect the concentration and purity of dsRNA with a 260/280 ratio of 1.8–2.0 and a total amount of dsRNA ≥ 50 μg. .. The specific primers used in this experimental procedure, including primers for the control gene green fluorescent protein (GFP), are enumerated in Table S1. dsRNA was injected into the thorax of CO2anesthetized 4th-instar nymphs using a Nikon microscope and a Narishige injection system (MN-151, Narishige). ..

Microscopy:

Article Title: Serotonin-mediated wing dimorphism in Nilaparvata lugens: A survival strategy triggered by host plant senescence.
Article Snippet: The Nanodrop 2000 was used to detect the concentration and purity of dsRNA with a 260/280 ratio of 1.8–2.0 and a total amount of dsRNA ≥ 50 μg. .. The specific primers used in this experimental procedure, including primers for the control gene green fluorescent protein (GFP), are enumerated in Table S1. dsRNA was injected into the thorax of CO2anesthetized 4th-instar nymphs using a Nikon microscope and a Narishige injection system (MN-151, Narishige). ..



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