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short, five-amino-acid sequence  (ImmunoGen Inc)

 
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    ImmunoGen Inc short, five-amino-acid sequence
    Short, Five Amino Acid Sequence, supplied by ImmunoGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunogen+sequence/short++five+amino+acid+sequence/pm39408855-75-14-33
    Average 90 stars, based on 1 article reviews
    short, five-amino-acid sequence - by Bioz Stars, 2026-11
    90/100 stars

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    other:

    Article Title: Accurate Visualization of C4d Complement Fragment in Immunohistochemistry by C-Terminal Linear Neoepitope-Specific Antibodies
    Article Snippet: The two pillars of our strategy were the following: (i) to use a short, five-amino-acid sequence that should constitute a linear neoepitope after the excision of the C4d fragment from iC4b, as an immunogen, and (ii) to use rabbits as the host organism, since rabbits’ immune system is able to generate a larger natural repertoire of high-affinity antibodies compared to mice and since it provides an improved immune response to small antigens [ ].

    Article Title: Accurate Visualization of C4d Complement Fragment in Immunohistochemistry by C-Terminal Linear Neoepitope-Specific Antibodies.
    Article Snippet: The two pillars of our strategy were the following: (i) to use a short, five-amino-acid sequence that should constitute a linear neoepitope after the excision of the C4d fragment from iC4b, as an immunogen, and (ii) to use rabbits as the host organism, since rabbits’ immune system is able to generate a larger natural repertoire of high-affinity antibodies compared to mice and since it provides an improved immune response to small antigens [22].



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    (A) An alignment of human PAX6 sequences reveals that all fifteen isoforms share the immunogenic sequence (highlighted in dark grey). NCBI sequence numbers are to the left of the sequences which are arranged alphabetically by isoform. (B) The four isoforms of axolotl PAX6 (sequences 1–4) contain the immunogenic sequence as does PAX6 from the Japanese fire-bellied newt (sequence 5) and the hypothetical protein from the Iberian ribbed newt (sequence 6). Sequence 7 is human PAX6. (C) Partial sequence obtained from E . nana containing 16/19 amino acids from the PAX6 immunogenic sequence.

    Journal: PLOS ONE

    Article Title: PAX6 protein in neuromasts of the lateral line system of salamanders ( Eurycea )

    doi: 10.1371/journal.pone.0293163

    Figure Lengend Snippet: (A) An alignment of human PAX6 sequences reveals that all fifteen isoforms share the immunogenic sequence (highlighted in dark grey). NCBI sequence numbers are to the left of the sequences which are arranged alphabetically by isoform. (B) The four isoforms of axolotl PAX6 (sequences 1–4) contain the immunogenic sequence as does PAX6 from the Japanese fire-bellied newt (sequence 5) and the hypothetical protein from the Iberian ribbed newt (sequence 6). Sequence 7 is human PAX6. (C) Partial sequence obtained from E . nana containing 16/19 amino acids from the PAX6 immunogenic sequence.

    Article Snippet: Primary antibody was diluted 1:10,000 in 5% non-fat dry milk in Tris-buffered saline with Tween20 (TBST), and half was incubated with custom peptide (Thermo Fisher) matching the antibody’s immunogenic sequence, resuspended in PBS at a concentration at least 5 times the molarity of the antibody.

    Techniques: Sequencing