dapi (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
dapi
Dapi, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunofluorescence+mounting+buffer+containing+dapi/DAPI/pm39701329-532-18-21
Average 98 stars, based on 2159 article reviews
Dapi, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunofluorescence+mounting+buffer+containing+dapi/DAPI/pm39701329-532-18-21
Average 98 stars, based on 2159 article reviews
dapi - by Bioz Stars,
2026-10
98/100 stars
Images
1) Product Images from "Irisin reprograms microglia through activation of STAT6 and prevents cognitive dysfunction after surgery in mice."
Article Title: Irisin reprograms microglia through activation of STAT6 and prevents cognitive dysfunction after surgery in mice.
Journal: Brain, behavior, and immunity
doi: 10.1016/j.bbi.2024.12.019
Figure Legend Snippet: Fig. 6. Inhibiting STAT6 phosphorylation weakens the effects of irisin. (A) Schematic of the experiment to intervene the effects of irisin by prophylactic admin- istration of AS (i.p.) in adult C57BL/6 mice. Mice with normal cognition measured by OPR were divided into groups. Immunofluorescence and ELISA analysis of dorsal hippocampus were performed on postoperative day 1. (B, C) Effect of intraperitoneal (i.p.) administered irisin or AS + irisin on OPR memory in LPS induced neuroinflammation mice. (mean ± s.e.m.; paired two-sided t-test, *P < 0.05, **P < 0.01, Test vs. Sample; Two-sided one-way ANOVA with Bonferroni post-hoc test, #P < 0.05; B, Before drug administration: **P = 0.0018, *P = 0.0441, *P = 0.0449, *P = 0.0336 for Naïve, LPS, Irisin + LPS and Irisin + AS + LPS, respectively. P > 0.9999, LPS vs. Naïve; P = 0.7748, Irisin + LPS vs. LPS; P > 0.9999, AS + Irisin + LPS vs. Irisin + LPS. n = 10 per group. C, 1d post-operation: *P = 0.0008, P = 0.7383, *P = 0.0150, P = 0.1215 for Naïve, LPS, Irisin + LPS and Irisin + AS + LPS, respectively. #P = 0.0142, Irisin + AS + LPS vs. Irisin + LPS; n = 10 per group). (D) ELISA analysis for the level of irisin in dHPC is shown. (mean ± s.e.m.; Two-sided one-way ANOVA with Bonferroni post-hoc test; P > 0.9999, LPS vs. Naïve; *P = 0.0274, Irisin + LPS vs. LPS; P = 0.0747, AS + Irisin + LPS vs. LPS; P > 0.9999, Irisin + AS + LPS vs. Irisin + LPS. n = 7 per group). (E) Representative confocal images of the dHPC stained with anti-Iba1 (red), pSTAT6 and pPDH (green), and NeuN (magenta) and DAPI (blue) in Naïve, LPS, Irisin + LPS and Irisin + AS + LPS. Scale bars, 50 and 10 µm. (F, G) ELISA analysis for the effect of each treatment on the pro-inflammatory factor IL-1β and the anti-inflammatory cytokine Arg-1 in dHPC is shown. (mean ± s.e.m.; Two-sided one-way ANOVA with Bonferroni post-hoc test; *P < 0.05, ****P < 0.0001; IL-1β: ****P < 0.0001, ****P < 0.0001, *P = 0.0457; Arg1: P > 0.9999, *P = 0.0183, *P = 0.0431 for LPS vs. Naïve, Irisin + LPS vs. LPS, and Irisin + AS + LPS vs. Irisin + LPS. n = 7 per group). See also Figures. S8. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Techniques Used: Phospho-proteomics, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Staining
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