dapi staining solution (Beyotime)
Beyotime is a verified supplier 
Beyotime manufactures this product 
99
Structured Review
Beyotime
dapi staining solution
Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2092 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunofluorescence+mounting+buffer+containing+dapi/DAPI+Staining+Solution/pmc13042531-249-2-44
Average 99 stars, based on 2092 article reviews
Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2092 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunofluorescence+mounting+buffer+containing+dapi/DAPI+Staining+Solution/pmc13042531-249-2-44
Average 99 stars, based on 2092 article reviews
dapi staining solution - by Bioz Stars,
2026-10
99/100 stars
Images
1) Product Images from "Beyond Catalytic Therapy: Copper‐Paeonol Nanozymes Disrupt Fascin‐Mediated Actin Bundling to Suppress Tumor Growth and Metastasis"
Article Title: Beyond Catalytic Therapy: Copper‐Paeonol Nanozymes Disrupt Fascin‐Mediated Actin Bundling to Suppress Tumor Growth and Metastasis
Journal: Advanced Science
doi: 10.1002/advs.202512186
Figure Legend Snippet: CuPaeNs bound to FSCN1. A) Bubble chart of GO enrichment analysis top 10 of cellular component after CuPaeNs treatment. B) Immunofluorescence staining of B16 cells in different groups. DAPI (blue), FSCN1 (green), and F‐actin (red). C) Molecular simulation of CuPaeNs. D) Computational model of active sites related to the potential key target of FSCN1 with paeonol, a single paeonol‐Cu 2+ unit, and CuPaeNs. The residues of the ligand‐binding domain proteins, involved in hydrogen bonds, were illustrated and marked with yellow dotted lines. E) Molecular simulation illustrating the binding affinity and detailed intermolecular binding interactions of FSCN1 with paeonol, a single Cu 2+ ‐paeonol unit, and CuPaeNs, focusing on main hydrogen bonds and hydrophobic interactions. F) Isothermal titration calorimetry result demonstrating the direct binding of CuPaeNs to FSCN1. G) CLSM images showing intracellular FSCN1 binding with CuPaeNs in B16 cells (scale bar = 20 µm) and H) responding pixel intensity plot. I) Assessment of CuPaeNs's impact on FSCN1's actin‐bundling activity using the Co‐IP assay. (J) Protein expression of FSCN1, F‐actin, and β‐actin with B16 cells in different groups, along with quantitative analysis ( n = 3). Statistically significant differences are indicated with their respective p ‐values ( *** p < 0.001).
Techniques Used: Immunofluorescence, Staining, Ligand Binding Assay, Binding Assay, Isothermal Titration Calorimetry, Activity Assay, Co-Immunoprecipitation Assay, Expressing
Related Articles
Labeling:Article Title: TRPM7 function in ischemia-reperfusion renal injury and its regulatory mechanism of PARthanatos Article Snippet: .. Upon completion of all labeling procedures, Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, DAPI Staining:Article Title: TRPM7 function in ischemia-reperfusion renal injury and its regulatory mechanism of PARthanatos Article Snippet: .. Upon completion of all labeling procedures, Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage. Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD. Staining:Article Title: TRPM7 function in ischemia-reperfusion renal injury and its regulatory mechanism of PARthanatos Article Snippet: .. Upon completion of all labeling procedures, Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique. Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique. Article Snippet: .. The nuclei of TE biopsy cells were then dyed by Article Title: Hierarchically sequential targeting nanoplatform suppresses NETosis-promoted angiogenesis for corneal neovascularization therapy. Article Snippet: The Phorbol 12-myristate 13-acetate (PMA, S1819) was purchased from Beyotime (Shanghai, China). .. The 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride Jo ur na l P re -p ro of ( Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, Article Title: Butyrate counteracts maternal obesity-induced cardiac defects by promoting PLEKHG3-actin binding Article Snippet: Vector TrueVIEW kit , Vector laboratories , SP-8400. .. Incubation:Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique. Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage. Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD. Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, Glass:Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique. Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in Inverted Microscopy:Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique. Article Snippet: .. The nuclei of TE biopsy cells were then dyed by Fluorescence:Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique. Article Snippet: .. The nuclei of TE biopsy cells were then dyed by other:Article Title: Lacticaseibacillus rhamnosus GG enhances gefitinib efficacy and reduces treatment-related toxicity via propionyl-L-carnitine regulation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Propionyl-L-carnitine (PLC) Aladdin Biochemical Technology, Shanghai, China CAS: 20064-19-1 Acetonitrile Xilong Scientific Co., Ltd., China CAS: 75-05-8 Formic acid Xilong Scientific Co., Ltd., China CAS: 64-18-6 Methotrexate Yuanye Bio-Technology, Shanghai, China CAS: 59-05-2 Luciferin sodium salt Yuanye Bio-Technology, Shanghai, China Cat#: B25437 Sodium carboxymethylcellulose (CMC-Na) Solarbio Life Sciences, Beijing, China CAS: 9004-32-4 Universal Tissue Fixative (Neutral) Servicebio, Wuhan, China Cat#: G1101 Trypsin Gibco Cat#: 25200-056 Fetal bovine serum Gibco Cat#: A5669701 Penicillin-streptomycin Gibco Cat#: 15140122 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#: 11965092 RPMI 1640 medium Gibco Cat#: C11875500BT BSA blocking solution Thermo Fisher Scientific Cat#: 37525 RIPA lysis buffer Thermo Fisher Scientific Cat#: Immunofluorescence:Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, Blocking Assay:Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, |