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181 immunofluorescence microscope  (Olympus)


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    Structured Review

    Olympus 181 immunofluorescence microscope
    181 Immunofluorescence Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 97/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunofluorescence+microscope/IXplore+Pro+Automated+Microscope+System/pm41720447-97-8-11
    Average 97 stars, based on 293 article reviews
    181 immunofluorescence microscope - by Bioz Stars, 2026-11
    97/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Proton signaling links epithelial sensing to neural control of host defense in C. elegans.
    Article Snippet: Primers (Tsingke Biotechnology Co., Ltd.) are listed in Supplementary Table 1. .. Imaging NHX-6::mStrawberry and ASIC-1::GFP translational fusion protein imaging was performed using an Olympus IXplore SpinSR confocal microscope with a 60×/1.42 NA oil immersion objective. .. All other fluorescence images were acquired using a Nikon Ts2R inverted microscope equipped with an Mshot MD60 CCD camera and the MShot Image Analysis System (version 1.1).

    Article Title: Proton signaling links epithelial sensing to neural control of host defense in C. elegans.
    Article Snippet: .. NHX-6::mStrawberry and ASIC-1::GFP translational fusion protein imaging was performed using an Olympus IXplore SpinSR confocal microscope with a 60×/1.42 NA oil immersion objective. ..

    Article Title: Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancer
    Article Snippet: Nuclei were counterstained with Antifade Mounting Medium with DAPI (#P0131, Beyotime, Shanghai, China) in the dark for 20 min. .. Finally, the stained samples were imaged and captured via either a laser-scanning confocal microscope (IXplore SpinSR, Olympus) or a Multi SIM X superresolution microscope (NanoInsights, Beijing, China) for superresolution imaging. ..

    Article Title: Cell-type-focused compound screen in human organoids reveals CK1 inhibition protects cone photoreceptors from death.
    Article Snippet: .. All non-screening imaging (Figures 1, 4G–4I, 5, 6, 7, S9, S11, S19–S21, S23, and S24) was conducted using a spinning disk confocal microscope (Olympus IXplore SpinSR) with a 4x or a 10x objective. ..

    Article Title: Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancer.
    Article Snippet: Following washing with PBS, the cell pellets were resuspended in PBS containing 10 μM Rhod-2 AM (#145037- 81-6, MedChemExpress, Shanghai, China) and incubated at room temperature for 30min in the dark. .. Finally, the stained samples were imaged and captured via either a laser-scanning confocal microscope (IXplore SpinSR, Olympus) or a Multi SIM X superresolution microscope (NanoInsights, Beijing, China) for superresolution imaging. ..

    Microscopy:

    Article Title: Proton signaling links epithelial sensing to neural control of host defense in C. elegans.
    Article Snippet: Primers (Tsingke Biotechnology Co., Ltd.) are listed in Supplementary Table 1. .. Imaging NHX-6::mStrawberry and ASIC-1::GFP translational fusion protein imaging was performed using an Olympus IXplore SpinSR confocal microscope with a 60×/1.42 NA oil immersion objective. .. All other fluorescence images were acquired using a Nikon Ts2R inverted microscope equipped with an Mshot MD60 CCD camera and the MShot Image Analysis System (version 1.1).

    Article Title: Membrane orientation regulates GAPR-1 oligomerization that is suitable for interaction with beclin 1
    Article Snippet: .. Samples were analyzed using an Olympus /Evident Spin Ixplore SoRa microscope with a 60x oil lens. ..

    Article Title: Proton signaling links epithelial sensing to neural control of host defense in C. elegans.
    Article Snippet: .. NHX-6::mStrawberry and ASIC-1::GFP translational fusion protein imaging was performed using an Olympus IXplore SpinSR confocal microscope with a 60×/1.42 NA oil immersion objective. ..

    Article Title: Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancer
    Article Snippet: Nuclei were counterstained with Antifade Mounting Medium with DAPI (#P0131, Beyotime, Shanghai, China) in the dark for 20 min. .. Finally, the stained samples were imaged and captured via either a laser-scanning confocal microscope (IXplore SpinSR, Olympus) or a Multi SIM X superresolution microscope (NanoInsights, Beijing, China) for superresolution imaging. ..

    Article Title: Tailored Porous Bimetallic Nanozyme Platform for Full-Cycle Therapeutics of Intestinal Ischemia/Reperfusion.
    Article Snippet: The next day, after washing with PBS, the sections were incubated with Alexa Fluor 594 or Alexa Fluor 488-labeled secondary antibodies at room temperature in the dark for 30 min. DAPI (Beyotime Biotechnology, C1099, China) counterstaining was used to visualize the nuclei. .. Immunofluorescent images were acquired via an Olympus microscope (IXplore SpinSR, Olympus Corporation, Tokyo, Japan) and analyzed using ImageJ software. ..

    Article Title: Inhibition of V-ATPase function drives apoptosis via GCN1/GCN2 kinase signaling
    Article Snippet: The following day, cells were stained with 100 nM LysoTrackerTM Red DND-99 (Invitrogen, Ref. 7528, Lot 1971192), 100 nM SiR-DNA (Spirochrome, 001_20.01, Lot 2401), or treated with 100 nM NoA-FITC. .. Images were acquired using an Olympus IXplore SpinSR microscope equipped with a 60x silicone oil objective (NA 1.3) and a SoRa Super-Resolution Spinning Disk system, providing a resolution of ∼150 nm. ..

    Article Title: Cell-type-focused compound screen in human organoids reveals CK1 inhibition protects cone photoreceptors from death.
    Article Snippet: .. All non-screening imaging (Figures 1, 4G–4I, 5, 6, 7, S9, S11, S19–S21, S23, and S24) was conducted using a spinning disk confocal microscope (Olympus IXplore SpinSR) with a 4x or a 10x objective. ..

    Article Title: Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancer.
    Article Snippet: Following washing with PBS, the cell pellets were resuspended in PBS containing 10 μM Rhod-2 AM (#145037- 81-6, MedChemExpress, Shanghai, China) and incubated at room temperature for 30min in the dark. .. Finally, the stained samples were imaged and captured via either a laser-scanning confocal microscope (IXplore SpinSR, Olympus) or a Multi SIM X superresolution microscope (NanoInsights, Beijing, China) for superresolution imaging. ..

    Staining:

    Article Title: Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancer
    Article Snippet: Nuclei were counterstained with Antifade Mounting Medium with DAPI (#P0131, Beyotime, Shanghai, China) in the dark for 20 min. .. Finally, the stained samples were imaged and captured via either a laser-scanning confocal microscope (IXplore SpinSR, Olympus) or a Multi SIM X superresolution microscope (NanoInsights, Beijing, China) for superresolution imaging. ..

    Article Title: Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancer.
    Article Snippet: Following washing with PBS, the cell pellets were resuspended in PBS containing 10 μM Rhod-2 AM (#145037- 81-6, MedChemExpress, Shanghai, China) and incubated at room temperature for 30min in the dark. .. Finally, the stained samples were imaged and captured via either a laser-scanning confocal microscope (IXplore SpinSR, Olympus) or a Multi SIM X superresolution microscope (NanoInsights, Beijing, China) for superresolution imaging. ..



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    Olympus immunofluorescence imaging
    IChondrocyte-sEVs promoted chondrogenesis. a Representative image of safranin O and Alcian blue staining of ATDC5 cells after 14 days of chondrogenesis induction in a 24-well plate (n = 5). b Proliferation curves of ATDC5 cells were monitored from day 1 to day 4 (n = 5). c Representative images of histopathological and <t>immunofluorescence</t> staining of MSCs following 21 days of chondrogenesis induction (n = 4). Scale bars: black = 100 um, white = 20 μm d Quantitative analysis of the average fluorescence intensity of COLII, ACAN and SOX9 based on the results shown in ( c ) (n = 4). e Relative mRNA expression levels of anabolic genes in MSCs after 21 days of chondrogenesis induction (n = 6). f Western blotting analysis of COLII, ACAN and SOX9 in MSCs after 21 days of chondrogenesis induction (n = 4). Data are presented as mean ± SD. ∗P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, ## P < 0.01 vs. control group.
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    Image Search Results


    IChondrocyte-sEVs promoted chondrogenesis. a Representative image of safranin O and Alcian blue staining of ATDC5 cells after 14 days of chondrogenesis induction in a 24-well plate (n = 5). b Proliferation curves of ATDC5 cells were monitored from day 1 to day 4 (n = 5). c Representative images of histopathological and immunofluorescence staining of MSCs following 21 days of chondrogenesis induction (n = 4). Scale bars: black = 100 um, white = 20 μm d Quantitative analysis of the average fluorescence intensity of COLII, ACAN and SOX9 based on the results shown in ( c ) (n = 4). e Relative mRNA expression levels of anabolic genes in MSCs after 21 days of chondrogenesis induction (n = 6). f Western blotting analysis of COLII, ACAN and SOX9 in MSCs after 21 days of chondrogenesis induction (n = 4). Data are presented as mean ± SD. ∗P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, ## P < 0.01 vs. control group.

    Journal: Journal of Orthopaedic Translation

    Article Title: Extracellular vesicles originating from induced pluripotent stem cell-derived chondrocytes facilitate the regeneration of osteoarthritic cartilage

    doi: 10.1016/j.jot.2025.101035

    Figure Lengend Snippet: IChondrocyte-sEVs promoted chondrogenesis. a Representative image of safranin O and Alcian blue staining of ATDC5 cells after 14 days of chondrogenesis induction in a 24-well plate (n = 5). b Proliferation curves of ATDC5 cells were monitored from day 1 to day 4 (n = 5). c Representative images of histopathological and immunofluorescence staining of MSCs following 21 days of chondrogenesis induction (n = 4). Scale bars: black = 100 um, white = 20 μm d Quantitative analysis of the average fluorescence intensity of COLII, ACAN and SOX9 based on the results shown in ( c ) (n = 4). e Relative mRNA expression levels of anabolic genes in MSCs after 21 days of chondrogenesis induction (n = 6). f Western blotting analysis of COLII, ACAN and SOX9 in MSCs after 21 days of chondrogenesis induction (n = 4). Data are presented as mean ± SD. ∗P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, ## P < 0.01 vs. control group.

    Article Snippet: The slices were sealed and stored at −20 °C for immunofluorescence imaging (FV 3000, Olympus, Japan).

    Techniques: Staining, Immunofluorescence, Fluorescence, Expressing, Western Blot, Control

    IChondrocyte-sEVs inhibited fibrosis and calcification in MSC-derived chondrocyte-pellets, as well as ameliorated impairment in OA chondrocytes. a Representative immunofluorescence images of MSC-derived chondrocytes pellets (n = 4). Scale bar: 20 μm b Quantitative analysis of mean fluorescence intensity for COLX and COLI across experimental groups (n = 4). c Microstructural characterization of MSC-derived chondrocyte pellets with μCT and SEM (n = 4). Calcified regions are indicated by green fluorescence, with calcium salt deposition marked by red arrows. Scale bar: white = 500 μm, black = 20 μm d Quantitative assessment of bone volume and bone volume percentage in MSC-derived chondrocytes pellets (n = 4). e OA chondrocytes proliferation was assessed over a 4-day period (n = 3). f Representative images illustrating cellular morphology, Safranin O staining, and Alcian blue staining of chondrocytes (n = 3). Scale bar:200 um. g Relative mRNA expression levels of COL2A1 and ACAN genes in chondrocytes (n = 6). h Western blotting analysis of COLII and ACAN in chondrocytes (n = 4). Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, ### P < 0.001 vs. control group.

    Journal: Journal of Orthopaedic Translation

    Article Title: Extracellular vesicles originating from induced pluripotent stem cell-derived chondrocytes facilitate the regeneration of osteoarthritic cartilage

    doi: 10.1016/j.jot.2025.101035

    Figure Lengend Snippet: IChondrocyte-sEVs inhibited fibrosis and calcification in MSC-derived chondrocyte-pellets, as well as ameliorated impairment in OA chondrocytes. a Representative immunofluorescence images of MSC-derived chondrocytes pellets (n = 4). Scale bar: 20 μm b Quantitative analysis of mean fluorescence intensity for COLX and COLI across experimental groups (n = 4). c Microstructural characterization of MSC-derived chondrocyte pellets with μCT and SEM (n = 4). Calcified regions are indicated by green fluorescence, with calcium salt deposition marked by red arrows. Scale bar: white = 500 μm, black = 20 μm d Quantitative assessment of bone volume and bone volume percentage in MSC-derived chondrocytes pellets (n = 4). e OA chondrocytes proliferation was assessed over a 4-day period (n = 3). f Representative images illustrating cellular morphology, Safranin O staining, and Alcian blue staining of chondrocytes (n = 3). Scale bar:200 um. g Relative mRNA expression levels of COL2A1 and ACAN genes in chondrocytes (n = 6). h Western blotting analysis of COLII and ACAN in chondrocytes (n = 4). Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, ### P < 0.001 vs. control group.

    Article Snippet: The slices were sealed and stored at −20 °C for immunofluorescence imaging (FV 3000, Olympus, Japan).

    Techniques: Derivative Assay, Immunofluorescence, Fluorescence, Staining, Expressing, Western Blot, Control