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immunofluorescence blocking solution  (Sangon Biotech)

 
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    Sangon Biotech immunofluorescence blocking solution
    The Effect of IGF2BP2 on the Proliferation and Differentiation of Myoblasts. (A) CCK-8 assay assessing cell proliferation following transfection with pcDNA3.1-IGF2BP2 or the negative control pcDNA3.1 at 0, 24, 48, 72, and 96 h (mean ± SEM; within each time point, significance between experimental group and control group was denoted as * P < 0.05; ** P < 0.01; n = 4). (B) EdU assay performed 48 h post-transfection to evaluate cell proliferation (20 ×, scale 50 µm) (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (C) The relative mRNA expression levels of IGF2BP2 and proliferation markers genes ( PCNA, CCND1 , and CDK2 ) 48 h after transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (D) Relative protein levels of proliferation markers ( PCNA and CDK2 ) 48 h after transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 3). (E) <t>Immunofluorescence</t> staining with MyHC (200 × magnification) after transfection. Cells were stained for MyHC in red serving as myogenic marker and DAPI in blue represents the nucleus. The right graph quantifies the relative myotube area (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (F) Relative mRNA expression levels of IGF2BP2 and myogenic differentiation markers, including MyHC, MyoD1, MyoG , and Myomaker after 3 days of differentiation induction (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (G) Relative protein levels of myogenic differentiation markers (MyHC, MyoD1) post-transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 3).
    Immunofluorescence Blocking Solution, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunofluorescence+blocking+solution/blocking+buffer+immunostaining/pmc13000497-150-6-9
    Average 86 stars, based on 1 article reviews
    immunofluorescence blocking solution - by Bioz Stars, 2026-11
    86/100 stars

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    1) Product Images from "Double-negative feedback loop between IGF2BP2 and miR-196-5p inhibits the proliferation and differentiation of primary chicken myoblasts"

    Article Title: Double-negative feedback loop between IGF2BP2 and miR-196-5p inhibits the proliferation and differentiation of primary chicken myoblasts

    Journal: Poultry Science

    doi: 10.1016/j.psj.2026.106746

    The Effect of IGF2BP2 on the Proliferation and Differentiation of Myoblasts. (A) CCK-8 assay assessing cell proliferation following transfection with pcDNA3.1-IGF2BP2 or the negative control pcDNA3.1 at 0, 24, 48, 72, and 96 h (mean ± SEM; within each time point, significance between experimental group and control group was denoted as * P < 0.05; ** P < 0.01; n = 4). (B) EdU assay performed 48 h post-transfection to evaluate cell proliferation (20 ×, scale 50 µm) (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (C) The relative mRNA expression levels of IGF2BP2 and proliferation markers genes ( PCNA, CCND1 , and CDK2 ) 48 h after transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (D) Relative protein levels of proliferation markers ( PCNA and CDK2 ) 48 h after transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 3). (E) Immunofluorescence staining with MyHC (200 × magnification) after transfection. Cells were stained for MyHC in red serving as myogenic marker and DAPI in blue represents the nucleus. The right graph quantifies the relative myotube area (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (F) Relative mRNA expression levels of IGF2BP2 and myogenic differentiation markers, including MyHC, MyoD1, MyoG , and Myomaker after 3 days of differentiation induction (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (G) Relative protein levels of myogenic differentiation markers (MyHC, MyoD1) post-transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 3).
    Figure Legend Snippet: The Effect of IGF2BP2 on the Proliferation and Differentiation of Myoblasts. (A) CCK-8 assay assessing cell proliferation following transfection with pcDNA3.1-IGF2BP2 or the negative control pcDNA3.1 at 0, 24, 48, 72, and 96 h (mean ± SEM; within each time point, significance between experimental group and control group was denoted as * P < 0.05; ** P < 0.01; n = 4). (B) EdU assay performed 48 h post-transfection to evaluate cell proliferation (20 ×, scale 50 µm) (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (C) The relative mRNA expression levels of IGF2BP2 and proliferation markers genes ( PCNA, CCND1 , and CDK2 ) 48 h after transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (D) Relative protein levels of proliferation markers ( PCNA and CDK2 ) 48 h after transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 3). (E) Immunofluorescence staining with MyHC (200 × magnification) after transfection. Cells were stained for MyHC in red serving as myogenic marker and DAPI in blue represents the nucleus. The right graph quantifies the relative myotube area (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (F) Relative mRNA expression levels of IGF2BP2 and myogenic differentiation markers, including MyHC, MyoD1, MyoG , and Myomaker after 3 days of differentiation induction (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (G) Relative protein levels of myogenic differentiation markers (MyHC, MyoD1) post-transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 3).

    Techniques Used: CCK-8 Assay, Transfection, Negative Control, Control, EdU Assay, Expressing, Immunofluorescence, Staining, Marker, Cell Characterization

    IGF2BP2 and miR-196-5p regulate chicken myoblasts differentiation. (A) Immunofluorescence analysis of MyHC cells after co-transfection (200x magnification) (mean ± SEM; n = 4). a, b Among different groups, values with different lowercase superscripts denote significant differences ( P < 0.05). (B) Relative mRNA expression of the myogenic differentiation markers (mean ± SEM; n = 4). a, b Among different groups, values with different lowercase superscripts denote significant differences ( P < 0.05). (C) Protein relative expression levels of myogenic differentiation markers. (mean ± SEM; n = 2). a, b Among different groups, values with different lowercase superscripts denote significant differences ( P < 0.05).
    Figure Legend Snippet: IGF2BP2 and miR-196-5p regulate chicken myoblasts differentiation. (A) Immunofluorescence analysis of MyHC cells after co-transfection (200x magnification) (mean ± SEM; n = 4). a, b Among different groups, values with different lowercase superscripts denote significant differences ( P < 0.05). (B) Relative mRNA expression of the myogenic differentiation markers (mean ± SEM; n = 4). a, b Among different groups, values with different lowercase superscripts denote significant differences ( P < 0.05). (C) Protein relative expression levels of myogenic differentiation markers. (mean ± SEM; n = 2). a, b Among different groups, values with different lowercase superscripts denote significant differences ( P < 0.05).

    Techniques Used: Immunofluorescence, Cotransfection, Expressing, Cell Characterization

    Related Articles

    Immunofluorescence:

    Article Title: Double-negative feedback loop between IGF2BP2 and miR-196-5p inhibits the proliferation and differentiation of primary chicken myoblasts
    Article Snippet: .. The cells were then blocked with immunofluorescence blocking solution (Sangon Biotechnology, Shanghai, China) for 1 h at room temperature. .. Subsequently, the cells were incubated with rabbit anti-MyHC primary antibody (1:2000, Proteintech, Wuhan, China) at 4°C overnight.

    Article Title: Double-negative feedback loop between IGF2BP2 and miR-196-5p inhibits the proliferation and differentiation of primary chicken myoblasts.
    Article Snippet: .. The cells were then blocked with immunofluorescence blocking solution (Sangon Biotechnology, Shanghai, China) for 1 h at room temperature. .. Subsequently, the cells were incubated with rabbit anti-MyHC primary antibody (1:2000, Proteintech, Wuhan, China) at 4◦C overnight.

    Blocking Assay:

    Article Title: Double-negative feedback loop between IGF2BP2 and miR-196-5p inhibits the proliferation and differentiation of primary chicken myoblasts
    Article Snippet: .. The cells were then blocked with immunofluorescence blocking solution (Sangon Biotechnology, Shanghai, China) for 1 h at room temperature. .. Subsequently, the cells were incubated with rabbit anti-MyHC primary antibody (1:2000, Proteintech, Wuhan, China) at 4°C overnight.

    Article Title: Blautia coccoides alleviates acute pancreatitis via bile salt hydrolase mediated deoxycholic acid production and farnesoid X receptor signaling.
    Article Snippet: After washing with 520 phosphate-buffered saline (PBS), the sections were outlined with a super pap pen (Biosharp, 521 China) and then treated with immunostaining blocking buffer (Sangon Biotech, China) for 1 522 hour at room temperature. .. Following the blocking step, tissues were incubated overnight at 4°C 523 with primary antibodies targeting claudin1 (ab211737; Abcam, USA), ZO-1 (13663; Cell 524 Signaling Technology, USA), or FGFR4 (11098-1-AP; Proteintech, China) prepared in primary 525 antibody dilution buffer (Sangon Biotech, China). .. The next day, slides were washed with PBS, 526 incubated for 1 hour at room temperature with Alexa Fluor 488/594 AffiniPure donkey anti-527 rabbit IgG (Yeason, China), and washed again.

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis
    Article Snippet: All original measurement and counting data were statistically analyzed and plotted with GraphPad Prism (GraphPad Software, USA). .. The samples were subsequently rehydrated in PBS, blocked with goat blocking buffer containing 5% goat serum (Cat. No. E674004, Cat. No. E510009, Sangon Biotech, China), and then incubated with primary antibodies at 4°C overnight, followed by incubation with secondary antibodies at 37°C for 1–2 h. The following primary antibodies were diluted into the above goat-blocking buffer for use: anti-HA high affinity (clone 3F10, rat monoclonal IgG, 1:50; Cat. No. 11867423001; RRID: AB_390918 , Roche, Switzerland), anti-acetylated α-tubulin (clone 6-11B-1, mouse monoclonal IgG, 1:200; Cat. No. T7451; RRID: AB_609894 , Sigma–Aldrich, USA), anti-NPHP4 (rabbit polyclonal IgG, 1:500; custom-made from ABclonal, China), and anti-NPHP8 (rabbit polyclonal IgG, 1:500; custom-made from ABclonal, China). .. The secondary antibodies were diluted in secondary antibody dilution buffer containing 5% goat serum (Cat. No. E674005, Cat. No. E510009, Sangon Biotech, China) for use: goat anti-mouse IgG H&L (Alexa Fluor 594), goat anti-rat IgG H&L (Alexa Fluor 488), and goat anti-rabbit IgG H&L (Alexa Fluor 488) (1:500; Cat. No. ab150120; RRID: AB_2631447 , Cat#ab150157; RRID: AB_2722511 , Cat. No. ab150077; RRID: AB_2630356 , Abcam, UK).

    Article Title: Faecalibacterium prausnitzii alleviates experimental recurrent acute pancreatitis by producing oleic acid to regulate MAPK/NF-κB signaling and Th17/Treg balance.
    Article Snippet: After repeated washing in PBS, a super pap pen (Sangon Biotech, China) was used to draw a circle around the tissue. .. Slides were blocked with immunostaining blocking buffer (Sangon Biotech, China) at room temperature for 1 h and incubated with primary antibodies against Claudin-1 and ZO-1 dilutedwith primary antibody dilution buffer (Sangon Biotech, China) at 4 °C overnight. .. Slides were washed with PBS and incubated with Alexa Fluor 488 AffiniPure donkey antirabbit IgG (Yeason, China) for 1 h at room temperature.

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis
    Article Snippet: DAPI-Fluoromount-G , SouthernBiotech , Cat#0100-20. .. Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , CatE674004. .. Immunostaining Secondary Antibody Dilution Buffer , Sangon Biotech , Cat#E674005.

    Article Title: Cellular transcriptomics reveals evolutionary adaptation and rumination of vertebrate stomachs
    Article Snippet: .. The sections in 1× citrate-based antigen retrieval solution (Sangon Biotech) were heated in a microwave oven, and stood still for 5 min, before being at 100°C for 5 min. After antigen retrieval, tissue sections were blocked with the Immunostaining Blocking Dilution Buffer (Sangon Biotech) for 1 h at room temperature. .. Subsequently, samples were incubated with primary antibody (1:500, Abcam, ab93279) overnight at 4°C.

    Article Title: Double-negative feedback loop between IGF2BP2 and miR-196-5p inhibits the proliferation and differentiation of primary chicken myoblasts.
    Article Snippet: .. The cells were then blocked with immunofluorescence blocking solution (Sangon Biotechnology, Shanghai, China) for 1 h at room temperature. .. Subsequently, the cells were incubated with rabbit anti-MyHC primary antibody (1:2000, Proteintech, Wuhan, China) at 4◦C overnight.

    Incubation:

    Article Title: Blautia coccoides alleviates acute pancreatitis via bile salt hydrolase mediated deoxycholic acid production and farnesoid X receptor signaling.
    Article Snippet: After washing with 520 phosphate-buffered saline (PBS), the sections were outlined with a super pap pen (Biosharp, 521 China) and then treated with immunostaining blocking buffer (Sangon Biotech, China) for 1 522 hour at room temperature. .. Following the blocking step, tissues were incubated overnight at 4°C 523 with primary antibodies targeting claudin1 (ab211737; Abcam, USA), ZO-1 (13663; Cell 524 Signaling Technology, USA), or FGFR4 (11098-1-AP; Proteintech, China) prepared in primary 525 antibody dilution buffer (Sangon Biotech, China). .. The next day, slides were washed with PBS, 526 incubated for 1 hour at room temperature with Alexa Fluor 488/594 AffiniPure donkey anti-527 rabbit IgG (Yeason, China), and washed again.

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis
    Article Snippet: All original measurement and counting data were statistically analyzed and plotted with GraphPad Prism (GraphPad Software, USA). .. The samples were subsequently rehydrated in PBS, blocked with goat blocking buffer containing 5% goat serum (Cat. No. E674004, Cat. No. E510009, Sangon Biotech, China), and then incubated with primary antibodies at 4°C overnight, followed by incubation with secondary antibodies at 37°C for 1–2 h. The following primary antibodies were diluted into the above goat-blocking buffer for use: anti-HA high affinity (clone 3F10, rat monoclonal IgG, 1:50; Cat. No. 11867423001; RRID: AB_390918 , Roche, Switzerland), anti-acetylated α-tubulin (clone 6-11B-1, mouse monoclonal IgG, 1:200; Cat. No. T7451; RRID: AB_609894 , Sigma–Aldrich, USA), anti-NPHP4 (rabbit polyclonal IgG, 1:500; custom-made from ABclonal, China), and anti-NPHP8 (rabbit polyclonal IgG, 1:500; custom-made from ABclonal, China). .. The secondary antibodies were diluted in secondary antibody dilution buffer containing 5% goat serum (Cat. No. E674005, Cat. No. E510009, Sangon Biotech, China) for use: goat anti-mouse IgG H&L (Alexa Fluor 594), goat anti-rat IgG H&L (Alexa Fluor 488), and goat anti-rabbit IgG H&L (Alexa Fluor 488) (1:500; Cat. No. ab150120; RRID: AB_2631447 , Cat#ab150157; RRID: AB_2722511 , Cat. No. ab150077; RRID: AB_2630356 , Abcam, UK).

    Article Title: Faecalibacterium prausnitzii alleviates experimental recurrent acute pancreatitis by producing oleic acid to regulate MAPK/NF-κB signaling and Th17/Treg balance.
    Article Snippet: After repeated washing in PBS, a super pap pen (Sangon Biotech, China) was used to draw a circle around the tissue. .. Slides were blocked with immunostaining blocking buffer (Sangon Biotech, China) at room temperature for 1 h and incubated with primary antibodies against Claudin-1 and ZO-1 dilutedwith primary antibody dilution buffer (Sangon Biotech, China) at 4 °C overnight. .. Slides were washed with PBS and incubated with Alexa Fluor 488 AffiniPure donkey antirabbit IgG (Yeason, China) for 1 h at room temperature.

    Immunostaining:

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis
    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004. .. Immunostaining Secondary Antibody Dilution Buffer , Sangon Biotech , CatE674005. .. Goat Serum , Sangon Biotech , Cat#E510009.

    Article Title: Faecalibacterium prausnitzii alleviates experimental recurrent acute pancreatitis by producing oleic acid to regulate MAPK/NF-κB signaling and Th17/Treg balance.
    Article Snippet: After repeated washing in PBS, a super pap pen (Sangon Biotech, China) was used to draw a circle around the tissue. .. Slides were blocked with immunostaining blocking buffer (Sangon Biotech, China) at room temperature for 1 h and incubated with primary antibodies against Claudin-1 and ZO-1 dilutedwith primary antibody dilution buffer (Sangon Biotech, China) at 4 °C overnight. .. Slides were washed with PBS and incubated with Alexa Fluor 488 AffiniPure donkey antirabbit IgG (Yeason, China) for 1 h at room temperature.

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis
    Article Snippet: DAPI-Fluoromount-G , SouthernBiotech , Cat#0100-20. .. Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , CatE674004. .. Immunostaining Secondary Antibody Dilution Buffer , Sangon Biotech , Cat#E674005.

    Article Title: Cellular transcriptomics reveals evolutionary adaptation and rumination of vertebrate stomachs
    Article Snippet: .. The sections in 1× citrate-based antigen retrieval solution (Sangon Biotech) were heated in a microwave oven, and stood still for 5 min, before being at 100°C for 5 min. After antigen retrieval, tissue sections were blocked with the Immunostaining Blocking Dilution Buffer (Sangon Biotech) for 1 h at room temperature. .. Subsequently, samples were incubated with primary antibody (1:500, Abcam, ab93279) overnight at 4°C.



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    The Effect of IGF2BP2 on the Proliferation and Differentiation of Myoblasts. (A) CCK-8 assay assessing cell proliferation following transfection with pcDNA3.1-IGF2BP2 or the negative control pcDNA3.1 at 0, 24, 48, 72, and 96 h (mean ± SEM; within each time point, significance between experimental group and control group was denoted as * P < 0.05; ** P < 0.01; n = 4). (B) EdU assay performed 48 h post-transfection to evaluate cell proliferation (20 ×, scale 50 µm) (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (C) The relative mRNA expression levels of IGF2BP2 and proliferation markers genes ( PCNA, CCND1 , and CDK2 ) 48 h after transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (D) Relative protein levels of proliferation markers ( PCNA and CDK2 ) 48 h after transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 3). (E) <t>Immunofluorescence</t> staining with MyHC (200 × magnification) after transfection. Cells were stained for MyHC in red serving as myogenic marker and DAPI in blue represents the nucleus. The right graph quantifies the relative myotube area (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (F) Relative mRNA expression levels of IGF2BP2 and myogenic differentiation markers, including MyHC, MyoD1, MyoG , and Myomaker after 3 days of differentiation induction (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (G) Relative protein levels of myogenic differentiation markers (MyHC, MyoD1) post-transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 3).
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    The Effect of IGF2BP2 on the Proliferation and Differentiation of Myoblasts. (A) CCK-8 assay assessing cell proliferation following transfection with pcDNA3.1-IGF2BP2 or the negative control pcDNA3.1 at 0, 24, 48, 72, and 96 h (mean ± SEM; within each time point, significance between experimental group and control group was denoted as * P < 0.05; ** P < 0.01; n = 4). (B) EdU assay performed 48 h post-transfection to evaluate cell proliferation (20 ×, scale 50 µm) (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (C) The relative mRNA expression levels of IGF2BP2 and proliferation markers genes ( PCNA, CCND1 , and CDK2 ) 48 h after transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (D) Relative protein levels of proliferation markers ( PCNA and CDK2 ) 48 h after transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 3). (E) Immunofluorescence staining with MyHC (200 × magnification) after transfection. Cells were stained for MyHC in red serving as myogenic marker and DAPI in blue represents the nucleus. The right graph quantifies the relative myotube area (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (F) Relative mRNA expression levels of IGF2BP2 and myogenic differentiation markers, including MyHC, MyoD1, MyoG , and Myomaker after 3 days of differentiation induction (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (G) Relative protein levels of myogenic differentiation markers (MyHC, MyoD1) post-transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 3).

    Journal: Poultry Science

    Article Title: Double-negative feedback loop between IGF2BP2 and miR-196-5p inhibits the proliferation and differentiation of primary chicken myoblasts

    doi: 10.1016/j.psj.2026.106746

    Figure Lengend Snippet: The Effect of IGF2BP2 on the Proliferation and Differentiation of Myoblasts. (A) CCK-8 assay assessing cell proliferation following transfection with pcDNA3.1-IGF2BP2 or the negative control pcDNA3.1 at 0, 24, 48, 72, and 96 h (mean ± SEM; within each time point, significance between experimental group and control group was denoted as * P < 0.05; ** P < 0.01; n = 4). (B) EdU assay performed 48 h post-transfection to evaluate cell proliferation (20 ×, scale 50 µm) (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (C) The relative mRNA expression levels of IGF2BP2 and proliferation markers genes ( PCNA, CCND1 , and CDK2 ) 48 h after transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (D) Relative protein levels of proliferation markers ( PCNA and CDK2 ) 48 h after transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 3). (E) Immunofluorescence staining with MyHC (200 × magnification) after transfection. Cells were stained for MyHC in red serving as myogenic marker and DAPI in blue represents the nucleus. The right graph quantifies the relative myotube area (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (F) Relative mRNA expression levels of IGF2BP2 and myogenic differentiation markers, including MyHC, MyoD1, MyoG , and Myomaker after 3 days of differentiation induction (mean ± SEM; * P < 0.05; ** P < 0.01; n = 4). (G) Relative protein levels of myogenic differentiation markers (MyHC, MyoD1) post-transfection (mean ± SEM; * P < 0.05; ** P < 0.01; n = 3).

    Article Snippet: The cells were then blocked with immunofluorescence blocking solution (Sangon Biotechnology, Shanghai, China) for 1 h at room temperature.

    Techniques: CCK-8 Assay, Transfection, Negative Control, Control, EdU Assay, Expressing, Immunofluorescence, Staining, Marker, Cell Characterization

    IGF2BP2 and miR-196-5p regulate chicken myoblasts differentiation. (A) Immunofluorescence analysis of MyHC cells after co-transfection (200x magnification) (mean ± SEM; n = 4). a, b Among different groups, values with different lowercase superscripts denote significant differences ( P < 0.05). (B) Relative mRNA expression of the myogenic differentiation markers (mean ± SEM; n = 4). a, b Among different groups, values with different lowercase superscripts denote significant differences ( P < 0.05). (C) Protein relative expression levels of myogenic differentiation markers. (mean ± SEM; n = 2). a, b Among different groups, values with different lowercase superscripts denote significant differences ( P < 0.05).

    Journal: Poultry Science

    Article Title: Double-negative feedback loop between IGF2BP2 and miR-196-5p inhibits the proliferation and differentiation of primary chicken myoblasts

    doi: 10.1016/j.psj.2026.106746

    Figure Lengend Snippet: IGF2BP2 and miR-196-5p regulate chicken myoblasts differentiation. (A) Immunofluorescence analysis of MyHC cells after co-transfection (200x magnification) (mean ± SEM; n = 4). a, b Among different groups, values with different lowercase superscripts denote significant differences ( P < 0.05). (B) Relative mRNA expression of the myogenic differentiation markers (mean ± SEM; n = 4). a, b Among different groups, values with different lowercase superscripts denote significant differences ( P < 0.05). (C) Protein relative expression levels of myogenic differentiation markers. (mean ± SEM; n = 2). a, b Among different groups, values with different lowercase superscripts denote significant differences ( P < 0.05).

    Article Snippet: The cells were then blocked with immunofluorescence blocking solution (Sangon Biotechnology, Shanghai, China) for 1 h at room temperature.

    Techniques: Immunofluorescence, Cotransfection, Expressing, Cell Characterization