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imc data  (fluidigm)


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    Structured Review

    fluidigm imc data
    Imc Data, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imc+data/Hyperion+Imaging+Mass+Cytometry+System/pm39535184-142-0-9
    Average 93 stars, based on 1523 article reviews
    imc data - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Insights into the architecture of earthworm metallothionein genes, powered by long-read genomics and transcriptomics
    Article Snippet: .. The tissue sections were imaged on the Hyperion imaging system (Standard Biotools). ..

    Article Title: Skin-derived myeloid precursors and joint-resident fibroblasts spread psoriatic disease from skin to joints
    Article Snippet: .. Acquisition was performed at 200 Hz on a Hyperion Imaging System (Standard Biotools, CYTOF software v. 7.1). ..

    Article Title: Multi-omics integration defines an ECM-associated intratumoral heterogeneity signature enabling prognosis assessment and therapeutic stratification in hepatocellular carcinoma.
    Article Snippet: Intratumoral heterogeneity (ITH) is a fundamental driver of hepatocellular carcinoma (HCC) progression and therapeutic resistance.. Here, we developed a multi-omics-derived ITH scoring system to improve patient stratification and guide precision therapy.. Using somatic mutation data from 361 TCGA HCC patients, we quantified genomic heterogeneity via the Mutant-Allele Tumor Heterogeneity (MATH) score, while single-cell RNA sequencing (scRNA-seq) of 20 HCC samples (our cohort and GSE149614) enabled assessment of cellular diversity.

    Article Title: Assessment of Imaging Mass Cytometry (IMC) as a Tool to Characterize Circulating Tumor Cells (CTCs) in Preclinical Mouse Models
    Article Snippet: .. Data were acquired by laser ablation with a Hyperion XTi Imaging System (Standard BioTools). ..

    Article Title: Tissue-scale mechanics controls differentiation strategy and dynamics of epithelial multilayering
    Article Snippet: Slides were then rinsed in 0.2% Triton X-100/PBS, stained with Cell-ID Intercalator-IR (Fluidigm #201192A) for 30 min at room temperature, washed in Milli-Q water, air-dried for 30 min, and stored until imaging. .. The samples were laser-ablated at 200 Hz using a Hyperion imaging mass cytometry system (Fluidigm) coupled to a Helios time-of-flight mass cytometer with a resolution of 1 μm. .. Slides were then rinsed in 0.2% Triton X-100/PBS, stained with Cell-ID Intercalator-IR (Fluidigm #201192A) for 30 min at room temperature, washed in Milli-Q water, air-dried for 30 min, and stored until imaging.

    Article Title: Treg-γδ T cell axis determines sexual dimorphism in hepatocarcinogenesis.
    Article Snippet: .. AR TI CL E IN P RE SS Imaging mass cytometry (IMC) and data processing Hyperion Mass Imaging System (Fluidigm Corporation, CA, USA) was conducted according to the tuning protocol provided by the manufacturer. ..

    Article Title: TERT drives liver tumorigenesis beyond telomere elongation
    Article Snippet: .. Imaging Mass Cytometry (IMC) was performed using the HyperionTM Imaging System (Standard BioTools, USA), a multiplexed spatial proteomics platform that enables simultaneous detection of multiple protein markers at subcellular resolution in formalin-fixed paraffin-embedded (FFPE) tissue sections. ..

    Software:

    Article Title: Skin-derived myeloid precursors and joint-resident fibroblasts spread psoriatic disease from skin to joints
    Article Snippet: .. Acquisition was performed at 200 Hz on a Hyperion Imaging System (Standard Biotools, CYTOF software v. 7.1). ..

    Cytometry:

    Article Title: Multi-omics integration defines an ECM-associated intratumoral heterogeneity signature enabling prognosis assessment and therapeutic stratification in hepatocellular carcinoma.
    Article Snippet: Intratumoral heterogeneity (ITH) is a fundamental driver of hepatocellular carcinoma (HCC) progression and therapeutic resistance.. Here, we developed a multi-omics-derived ITH scoring system to improve patient stratification and guide precision therapy.. Using somatic mutation data from 361 TCGA HCC patients, we quantified genomic heterogeneity via the Mutant-Allele Tumor Heterogeneity (MATH) score, while single-cell RNA sequencing (scRNA-seq) of 20 HCC samples (our cohort and GSE149614) enabled assessment of cellular diversity.

    Article Title: Tissue-scale mechanics controls differentiation strategy and dynamics of epithelial multilayering
    Article Snippet: Slides were then rinsed in 0.2% Triton X-100/PBS, stained with Cell-ID Intercalator-IR (Fluidigm #201192A) for 30 min at room temperature, washed in Milli-Q water, air-dried for 30 min, and stored until imaging. .. The samples were laser-ablated at 200 Hz using a Hyperion imaging mass cytometry system (Fluidigm) coupled to a Helios time-of-flight mass cytometer with a resolution of 1 μm. .. Slides were then rinsed in 0.2% Triton X-100/PBS, stained with Cell-ID Intercalator-IR (Fluidigm #201192A) for 30 min at room temperature, washed in Milli-Q water, air-dried for 30 min, and stored until imaging.

    Mass Cytometry:

    Article Title: Tissue-scale mechanics controls differentiation strategy and dynamics of epithelial multilayering
    Article Snippet: Slides were then rinsed in 0.2% Triton X-100/PBS, stained with Cell-ID Intercalator-IR (Fluidigm #201192A) for 30 min at room temperature, washed in Milli-Q water, air-dried for 30 min, and stored until imaging. .. The samples were laser-ablated at 200 Hz using a Hyperion imaging mass cytometry system (Fluidigm) coupled to a Helios time-of-flight mass cytometer with a resolution of 1 μm. .. Slides were then rinsed in 0.2% Triton X-100/PBS, stained with Cell-ID Intercalator-IR (Fluidigm #201192A) for 30 min at room temperature, washed in Milli-Q water, air-dried for 30 min, and stored until imaging.

    Article Title: Treg-γδ T cell axis determines sexual dimorphism in hepatocarcinogenesis.
    Article Snippet: .. AR TI CL E IN P RE SS Imaging mass cytometry (IMC) and data processing Hyperion Mass Imaging System (Fluidigm Corporation, CA, USA) was conducted according to the tuning protocol provided by the manufacturer. ..

    Article Title: TERT drives liver tumorigenesis beyond telomere elongation
    Article Snippet: .. Imaging Mass Cytometry (IMC) was performed using the HyperionTM Imaging System (Standard BioTools, USA), a multiplexed spatial proteomics platform that enables simultaneous detection of multiple protein markers at subcellular resolution in formalin-fixed paraffin-embedded (FFPE) tissue sections. ..

    other:

    Article Title: Skin-derived myeloid precursors and joint-resident fibroblasts spread psoriatic disease from skin to joints.
    Article Snippet: Image analysis was performed using image-based algorithms in ImageStream Data Exploration and Analysis Software (IDEAS 6.2.189, Cytek Biosciences).

    Spatial Proteomics:

    Article Title: TERT drives liver tumorigenesis beyond telomere elongation
    Article Snippet: .. Imaging Mass Cytometry (IMC) was performed using the HyperionTM Imaging System (Standard BioTools, USA), a multiplexed spatial proteomics platform that enables simultaneous detection of multiple protein markers at subcellular resolution in formalin-fixed paraffin-embedded (FFPE) tissue sections. ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: TERT drives liver tumorigenesis beyond telomere elongation
    Article Snippet: .. Imaging Mass Cytometry (IMC) was performed using the HyperionTM Imaging System (Standard BioTools, USA), a multiplexed spatial proteomics platform that enables simultaneous detection of multiple protein markers at subcellular resolution in formalin-fixed paraffin-embedded (FFPE) tissue sections. ..



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    ( A ) The imaging mass cytometry <t>(IMC)</t> <t>data</t> collection and analysis workflow outlines the steps involved in gaining comprehensive insights into the spatial distribution of immune cells and relevant cell types within undecidualized (UnD) and decidualized (DD) EM-like lesions of WT, CNR1 k/o, and CNR2 k/o mice. ( B, C ) Representative images showing the single-cell segmentation performed following the acquisition of two regions of interest (ROI) per section (three biological samples per genotype) and segmentation quality of the data after segmentation analysis was conducted, respectively. ( D ) Non-linear dimensionality reduction after batch effect correction showed distinct expression patterns of immune cells and cell state markers between UnD and DD lesions. DD lesions from the CNR1 k/o and CNR2 k/o mice showed expression pattern that was significantly different from the DD lesions of WT mice, as well as compared to UnD lesions among different genotypes. ( E ) Uniform manifold approximation and projection (UMAP) dimensionality reduction highlighted key cell types and differences in composition between UnD and DD lesions. DD lesions exhibited increased stroma and fibroblasts, decreased epithelial cells, and heightened macrophage infiltration compared to UnD lesions.
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    Image Search Results


    ( A ) The imaging mass cytometry (IMC) data collection and analysis workflow outlines the steps involved in gaining comprehensive insights into the spatial distribution of immune cells and relevant cell types within undecidualized (UnD) and decidualized (DD) EM-like lesions of WT, CNR1 k/o, and CNR2 k/o mice. ( B, C ) Representative images showing the single-cell segmentation performed following the acquisition of two regions of interest (ROI) per section (three biological samples per genotype) and segmentation quality of the data after segmentation analysis was conducted, respectively. ( D ) Non-linear dimensionality reduction after batch effect correction showed distinct expression patterns of immune cells and cell state markers between UnD and DD lesions. DD lesions from the CNR1 k/o and CNR2 k/o mice showed expression pattern that was significantly different from the DD lesions of WT mice, as well as compared to UnD lesions among different genotypes. ( E ) Uniform manifold approximation and projection (UMAP) dimensionality reduction highlighted key cell types and differences in composition between UnD and DD lesions. DD lesions exhibited increased stroma and fibroblasts, decreased epithelial cells, and heightened macrophage infiltration compared to UnD lesions.

    Journal: eLife

    Article Title: Endocannabinoids and their receptors modulate endometriosis pathogenesis and immune response

    doi: 10.7554/eLife.96523

    Figure Lengend Snippet: ( A ) The imaging mass cytometry (IMC) data collection and analysis workflow outlines the steps involved in gaining comprehensive insights into the spatial distribution of immune cells and relevant cell types within undecidualized (UnD) and decidualized (DD) EM-like lesions of WT, CNR1 k/o, and CNR2 k/o mice. ( B, C ) Representative images showing the single-cell segmentation performed following the acquisition of two regions of interest (ROI) per section (three biological samples per genotype) and segmentation quality of the data after segmentation analysis was conducted, respectively. ( D ) Non-linear dimensionality reduction after batch effect correction showed distinct expression patterns of immune cells and cell state markers between UnD and DD lesions. DD lesions from the CNR1 k/o and CNR2 k/o mice showed expression pattern that was significantly different from the DD lesions of WT mice, as well as compared to UnD lesions among different genotypes. ( E ) Uniform manifold approximation and projection (UMAP) dimensionality reduction highlighted key cell types and differences in composition between UnD and DD lesions. DD lesions exhibited increased stroma and fibroblasts, decreased epithelial cells, and heightened macrophage infiltration compared to UnD lesions.

    Article Snippet: Bulk mRNA sequencing data is provided in the supplementary files (Supplementary Data 4) and IMC data generated in this study has been deposited in Mendeley Data ( https://doi.org/10.17632/2ptns5yhzh.2 ).

    Techniques: Imaging, Mass Cytometry, Expressing