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Becton Dickinson imag buffer
Imag Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Characterization of ovarian progenitor cells for their potential to generate steroidogenic theca cells in vitro
Article Snippet: Anti-R-phycoerythrin (PE) Magnetic Particles and BD IMag Buffer (10×) were from BD Biosciences (Franklin Lakes, NJ, USA).

Article Title: Downregulation of circular RNA ETS1 promotes SLE activity and inhibits Treg cell differentiation through miR-1205/FoxP3 molecular axis.
Article Snippet: The supernatant was discarded and samples were resuspended with 500 μL BD IMag™ Buffer.

Article Title: Endothelial cell expression of a STING gain-of-function mutation initiates pulmonary lymphocytic infiltration
Article Snippet: BD IMag Buffer (10X) , BD Biosciences , Cat#552362.

Article Title: Isolation of Leydig cells from adult rat testes by magnetic-activated cell sorting protocol based on prolactin receptor expression.
Article Snippet: 2Department of Gynecology andObstetrics, The Second Affiliated Hospital and Yuying Children’s Hospital ofWenzhouMedical University,Wenzhou, Zhejiang, China 3Department of Pharmacology, The Second Affiliated Hospital and Yuying Children’s Hospital ofWenzhouMedical University,Wenzhou, Zhejiang, China 4The BasicMedical Research Center of the Second School ofMedicine, The Second Affiliated Hospital and Yuying Children’s Hospital ofWenzhouMedical University, Wenzhou, Zhejiang, China. Congde Chen, Key Laboratory of Children Genitourinary Diseases ofWenzhou City, Department of Pediatric Urology, The Second Affiliated Hospital and Yuying Children’s Hospital ofWenzhouMedical University, Wenzhou, Zhejiang 325027, China.. Email: chencd@wmu.edu.cn Haolin Chen, Zhejiang Provincial Key Laboratory of Anesthesiology, Department of Anesthesiology, The Second Affiliated Hospital and Yuying Children’s Hospital ofWenzhou Medical University,Wenzhou, Zhejiang 325027, China.

Article Title: Downregulation of circular RNA ETS1 promotes SLE activity and inhibits Treg cell differentiation through miR-1205/FoxP3 molecular axis.
Article Snippet: The tube was installed on a BD IMagnetTM, and 2 mL of BD IMagTM Buffer was added.

Centrifugation:

Article Title: Myeloid cell-specific ablation of Runx2 gene exacerbates post-infarct cardiac remodeling.
Article Snippet: Biotinylated anti-CD11b antibody (BioLegend, 101203) was added to cell suspension (1 μl / 1 × 106 cells) and incubated for 30 min on ice. .. After centrifugation, the cells were resuspended in BD IMag Buffer (BD Biosciences, 552362), Streptavidin Particles Plus-DM (BD Biosciences, 557812) was added five times the amount of biotin anti-CD11b antibody, then incubated for 30 min on ice. ..

Article Title: Myeloid cell-specific ablation of Runx2 gene exacerbates post-infarct cardiac remodeling
Article Snippet: Biotinylated anti-CD11b antibody (BioLegend, 101203) was added to cell suspension (1 μl / 1 × 10 6 cells) and incubated for 30 min on ice. .. After centrifugation, the cells were resuspended in BD IMag Buffer (BD Biosciences, 552362), Streptavidin Particles Plus-DM (BD Biosciences, 557812) was added five times the amount of biotin anti-CD11b antibody, then incubated for 30 min on ice. ..

Incubation:

Article Title: Myeloid cell-specific ablation of Runx2 gene exacerbates post-infarct cardiac remodeling.
Article Snippet: Biotinylated anti-CD11b antibody (BioLegend, 101203) was added to cell suspension (1 μl / 1 × 106 cells) and incubated for 30 min on ice. .. After centrifugation, the cells were resuspended in BD IMag Buffer (BD Biosciences, 552362), Streptavidin Particles Plus-DM (BD Biosciences, 557812) was added five times the amount of biotin anti-CD11b antibody, then incubated for 30 min on ice. ..

Article Title: Myeloid cell-specific ablation of Runx2 gene exacerbates post-infarct cardiac remodeling
Article Snippet: Biotinylated anti-CD11b antibody (BioLegend, 101203) was added to cell suspension (1 μl / 1 × 10 6 cells) and incubated for 30 min on ice. .. After centrifugation, the cells were resuspended in BD IMag Buffer (BD Biosciences, 552362), Streptavidin Particles Plus-DM (BD Biosciences, 557812) was added five times the amount of biotin anti-CD11b antibody, then incubated for 30 min on ice. ..



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a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Journal: bioRxiv

Article Title: Mouse behavioral genomics identifies Creld1 as a gatekeeper of somatosensation

doi: 10.64898/2026.03.30.715210

Figure Lengend Snippet: a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: In brief, cells were washed with the Ca 2+ imaging buffer (1 x HBSS buffer with 1.3 mM Ca 2+ , 10 mM HEPES, pH 7.2) and then loaded with the Ca 2+ imaging buffer containing 2.5 mM Ca 2+ indicator dye Fura-2, AM (ThermoFisher scientific, F1225) and 0.05% Pluronic F-127 (Beyotime, ST501).

Techniques: CRISPR, Knock-Out, Injection, Virus, Construct, Infection, Single Cell, Imaging, Derivative Assay, Control, Cell Culture, Randall–Selitto Test, Hot Plate Test