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imagequanttm las 4000 software  (Danaher Inc)


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    Structured Review

    Danaher Inc imagequanttm las 4000 software
    Imagequanttm Las 4000 Software, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 97/100, based on 6553 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagequanttl+software/ImageQuant+LAS+4000/pmc10299407-147-5-9
    Average 97 stars, based on 6553 article reviews
    imagequanttm las 4000 software - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Tyrosine Kinase Inhibitors Diminish Renal Neoplasms in a Tuberous Sclerosis Model Via Induction of Apoptosis
    Article Snippet: .. All membranes were washed with TBS-T, incubated with peroxidase (POD)-labeled secondary antibodies at room temperature, and visualized with Pierce ECL reagent (No. 32106, Thermo Fisher Scientific) and ImageQuant LAS 4000 Series imager (GE Healthcare Life Sciences). ..

    Article Title: Adipocytes promote pancreatic cancer migration and invasion through fatty acid metabolic reprogramming
    Article Snippet: A total of 20 μg protein was loaded per lane onto 12% SDS-gels, resolved using SDS-PAGE, transferred to 0.22 μm PVDF membranes (MilliporeSigma), and blocked for 1 h at room temperature with 5% skim milk. .. Each membrane was immunoblotted with the indicated primary antibodies at 4°C overnight and then incubated with a secondary antibody at 37°C for 1 h. Immunoreactive bands were visualized using an enhanced chemiluminescence detection kit (Thermo Fisher Scientific, Inc.) using an ImageQuant LAS 4000 System (GE Healthcare). .. The following primary and secondary antibodies were used in the present study: AKT (1:1,000; ProteinTech Group, Inc.; cat. no. 10176-2-AP), p-AKT Ser473 (1:1,000, Cell Signaling Technology, Inc.; cat. no. CST4060S), HIF-1α (1:1,000; ProteinTech Group, Inc.; cat. no. 20960-1-AP), ANGPTL4 (1:1,000; ProteinTech Group, Inc.; cat. no. 18374-1-AP), GLUT1 (1:1,000; ProteinTech Group, Inc.; cat. no. 66290-1-lg), HK2 (1:1,000; ProteinTech Group, Inc.; cat. no. 22029-1-AP), LDHA (1:1,000; ProteinTech Group, Inc.; cat. no. 19987-1-AP), α-tubulin (1:1,000; ProteinTech Group, Inc.; cat. no. 66031-1-lg), p-AMPKα Thr172 (1:1,000; Cell Signaling Technology, Inc.; cat. no. CST2535), AMPKα (1:1,000; ProteinTech Group, Inc.; cat. no. 10929-2-AP), p-STAT3 Tyr705 (1:1,000; Cell Signaling Technology, Inc.; cat. no. CST9145), STAT3 (1:1,000; Cell Signaling Technology, Inc.; CST12640), Snail (1:1,000; CST; CST9782), horseradish peroxidase-conjugated anti-rabbit IgG antibody (1:5,000; ProteinTech Group, Inc.; cat. no. SA00001-2) and goat anti-mouse IgG secondary antibody HRP conjugated (1:5,000; Signalway Antibody LLC; cat. no. L3032).

    Western Blot:

    Article Title: A leaf-emanated signal orchestrates grain size and number in response to maternal resources.
    Article Snippet: .. Signals were developed and detected using ECL Prime Western Blotting Detection Reagent and ImageQuant LAS 4000 (both GE Healthcare Life Sciences). ..

    Article Title: Selective Inhibition of ATM-dependent Double-strand Break Repair and Checkpoint Control Synergistically Enhances the Efficacy of ATR Inhibitors
    Article Snippet: The primary antibodies used were: p-ATM S1981 (Abcam #ab81292), ATM (Santa Cruz Biotechnology #SC23921), p-KAP1 S824 (Abcam #ab133440), KAP1 (Abcam #ab22553), p-CHK2 T68 (Cell Signaling Technology #2197), CHK2 (Cell Signaling Technology #6334), p-CHK1 S317 (Cell Signaling Technology #12302), CHK1 (Cell Signaling Technology #2360), p-p53 S15 (Cell Signaling Technology #9284), p53 (Cell Signaling Technology #48818), MDM2 (Cell Signaling Technology #86934), p21 (Cell Signaling Technology #2947), and horseradish peroxidase–conjugated GAPDH (Cell Signaling Technology #3683). .. Western blots were analyzed using GE ImageQuant LAS 4000 with SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific #34080) and/or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific #34094). .. Cell-cycle analysis was performed using the FITC bromodeoxyuridine (BrdU) Flow Kit from BD Biosciences (#559619) according to the manufacturer's protocol.

    Membrane:

    Article Title: Adipocytes promote pancreatic cancer migration and invasion through fatty acid metabolic reprogramming
    Article Snippet: A total of 20 μg protein was loaded per lane onto 12% SDS-gels, resolved using SDS-PAGE, transferred to 0.22 μm PVDF membranes (MilliporeSigma), and blocked for 1 h at room temperature with 5% skim milk. .. Each membrane was immunoblotted with the indicated primary antibodies at 4°C overnight and then incubated with a secondary antibody at 37°C for 1 h. Immunoreactive bands were visualized using an enhanced chemiluminescence detection kit (Thermo Fisher Scientific, Inc.) using an ImageQuant LAS 4000 System (GE Healthcare). .. The following primary and secondary antibodies were used in the present study: AKT (1:1,000; ProteinTech Group, Inc.; cat. no. 10176-2-AP), p-AKT Ser473 (1:1,000, Cell Signaling Technology, Inc.; cat. no. CST4060S), HIF-1α (1:1,000; ProteinTech Group, Inc.; cat. no. 20960-1-AP), ANGPTL4 (1:1,000; ProteinTech Group, Inc.; cat. no. 18374-1-AP), GLUT1 (1:1,000; ProteinTech Group, Inc.; cat. no. 66290-1-lg), HK2 (1:1,000; ProteinTech Group, Inc.; cat. no. 22029-1-AP), LDHA (1:1,000; ProteinTech Group, Inc.; cat. no. 19987-1-AP), α-tubulin (1:1,000; ProteinTech Group, Inc.; cat. no. 66031-1-lg), p-AMPKα Thr172 (1:1,000; Cell Signaling Technology, Inc.; cat. no. CST2535), AMPKα (1:1,000; ProteinTech Group, Inc.; cat. no. 10929-2-AP), p-STAT3 Tyr705 (1:1,000; Cell Signaling Technology, Inc.; cat. no. CST9145), STAT3 (1:1,000; Cell Signaling Technology, Inc.; CST12640), Snail (1:1,000; CST; CST9782), horseradish peroxidase-conjugated anti-rabbit IgG antibody (1:5,000; ProteinTech Group, Inc.; cat. no. SA00001-2) and goat anti-mouse IgG secondary antibody HRP conjugated (1:5,000; Signalway Antibody LLC; cat. no. L3032).



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