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ecl luminescent substrate  (Danaher Inc)


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    Structured Review

    Danaher Inc ecl luminescent substrate
    Ecl Luminescent Substrate, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 97/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagequant+800+system/Amersham+ImageQuant+800+UV/pmc09520331-72-6-9
    Average 97 stars, based on 7 article reviews
    ecl luminescent substrate - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Purification:

    Article Title: Hierarchical small molecule inhibition of MYST acetyltransferases
    Article Snippet: ling Technology, #7003) or SuperSignal ELIZA Femto Substrate (Thermo Scientific, 37074) according to the manufacturer’s protocols. Images were captured using an Amersham ImageQuant 800 imaging system (Cytiva, 29399482). The preparation of magnetic bead H3K14-CoA affinity resin was adapted from a previously reported protocol 54 . Briefly, 15.5 mg (1 eq) of a purified peptide with the linear sequence

    Article Title: Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
    Article Snippet: The membrane was incubated with Bcl-2 (1:1,000; cat. no. 4223; Cell Signaling Technology, Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature.Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature. ... The protein bands were visualized by ECL luminescent substrate (Cytiva, Amersham ImageQuant 800UV, USA), and the results were analyzed by ImageJ software.

    Article Title: Protective effect of liquiritin on coronary heart disease through regulating the proliferation of human vascular smooth muscle cells via upregulation of sirtuin1
    Article Snippet: The membrane was incubated with Bcl-2 (cat. no. 4223; 1: 1000; Cell Signaling Technology, Inc.), Bax (cat. no. 5023; 1: 1000; Cell Signaling Technology, Inc.), MMP9 (cat. no. 13,667; 1: 1000; Cell Signaling Technology, Inc.), SIRT1 (cat. no. 2496; 1: 1000; Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature.Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature. ... The protein bands were visualized using enhanced chemiluminescence luminescent substrate (Amersham ImageQuant800UV; Cytiva, MA, USA) and quantified using ImageJ software.

    Imaging:

    Article Title: Hierarchical small molecule inhibition of MYST acetyltransferases
    Article Snippet: ling Technology, #7003) or SuperSignal ELIZA Femto Substrate (Thermo Scientific, 37074) according to the manufacturer’s protocols. Images were captured using an Amersham ImageQuant 800 imaging system (Cytiva, 29399482). The preparation of magnetic bead H3K14-CoA affinity resin was adapted from a previously reported protocol 54 . Briefly, 15.5 mg (1 eq) of a purified peptide with the linear sequence

    Article Title: Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
    Article Snippet: The membrane was incubated with Bcl-2 (1:1,000; cat. no. 4223; Cell Signaling Technology, Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature.Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature. ... The protein bands were visualized by ECL luminescent substrate (Cytiva, Amersham ImageQuant 800UV, USA), and the results were analyzed by ImageJ software.

    Article Title: Protective effect of liquiritin on coronary heart disease through regulating the proliferation of human vascular smooth muscle cells via upregulation of sirtuin1
    Article Snippet: The membrane was incubated with Bcl-2 (cat. no. 4223; 1: 1000; Cell Signaling Technology, Inc.), Bax (cat. no. 5023; 1: 1000; Cell Signaling Technology, Inc.), MMP9 (cat. no. 13,667; 1: 1000; Cell Signaling Technology, Inc.), SIRT1 (cat. no. 2496; 1: 1000; Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature.Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature. ... The protein bands were visualized using enhanced chemiluminescence luminescent substrate (Amersham ImageQuant800UV; Cytiva, MA, USA) and quantified using ImageJ software.

    Sequencing:

    Article Title: Hierarchical small molecule inhibition of MYST acetyltransferases
    Article Snippet: ling Technology, #7003) or SuperSignal ELIZA Femto Substrate (Thermo Scientific, 37074) according to the manufacturer’s protocols. Images were captured using an Amersham ImageQuant 800 imaging system (Cytiva, 29399482). The preparation of magnetic bead H3K14-CoA affinity resin was adapted from a previously reported protocol 54 . Briefly, 15.5 mg (1 eq) of a purified peptide with the linear sequence

    Article Title: Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
    Article Snippet: The membrane was incubated with Bcl-2 (1:1,000; cat. no. 4223; Cell Signaling Technology, Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature.Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature. ... The protein bands were visualized by ECL luminescent substrate (Cytiva, Amersham ImageQuant 800UV, USA), and the results were analyzed by ImageJ software.

    Article Title: Protective effect of liquiritin on coronary heart disease through regulating the proliferation of human vascular smooth muscle cells via upregulation of sirtuin1
    Article Snippet: The membrane was incubated with Bcl-2 (cat. no. 4223; 1: 1000; Cell Signaling Technology, Inc.), Bax (cat. no. 5023; 1: 1000; Cell Signaling Technology, Inc.), MMP9 (cat. no. 13,667; 1: 1000; Cell Signaling Technology, Inc.), SIRT1 (cat. no. 2496; 1: 1000; Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature.Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature. ... The protein bands were visualized using enhanced chemiluminescence luminescent substrate (Amersham ImageQuant800UV; Cytiva, MA, USA) and quantified using ImageJ software.

    Membrane:

    Article Title: Hierarchical small molecule inhibition of MYST acetyltransferases
    Article Snippet: ling Technology, #7003) or SuperSignal ELIZA Femto Substrate (Thermo Scientific, 37074) according to the manufacturer’s protocols. Images were captured using an Amersham ImageQuant 800 imaging system (Cytiva, 29399482). The preparation of magnetic bead H3K14-CoA affinity resin was adapted from a previously reported protocol 54 . Briefly, 15.5 mg (1 eq) of a purified peptide with the linear sequence

    Article Title: Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
    Article Snippet: The membrane was incubated with Bcl-2 (1:1,000; cat. no. 4223; Cell Signaling Technology, Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature.Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature. ... The protein bands were visualized by ECL luminescent substrate (Cytiva, Amersham ImageQuant 800UV, USA), and the results were analyzed by ImageJ software.

    Article Title: Protective effect of liquiritin on coronary heart disease through regulating the proliferation of human vascular smooth muscle cells via upregulation of sirtuin1
    Article Snippet: The membrane was incubated with Bcl-2 (cat. no. 4223; 1: 1000; Cell Signaling Technology, Inc.), Bax (cat. no. 5023; 1: 1000; Cell Signaling Technology, Inc.), MMP9 (cat. no. 13,667; 1: 1000; Cell Signaling Technology, Inc.), SIRT1 (cat. no. 2496; 1: 1000; Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature.Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature. ... The protein bands were visualized using enhanced chemiluminescence luminescent substrate (Amersham ImageQuant800UV; Cytiva, MA, USA) and quantified using ImageJ software.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Hierarchical small molecule inhibition of MYST acetyltransferases
    Article Snippet: ling Technology, #7003) or SuperSignal ELIZA Femto Substrate (Thermo Scientific, 37074) according to the manufacturer’s protocols. Images were captured using an Amersham ImageQuant 800 imaging system (Cytiva, 29399482). The preparation of magnetic bead H3K14-CoA affinity resin was adapted from a previously reported protocol 54 . Briefly, 15.5 mg (1 eq) of a purified peptide with the linear sequence

    Article Title: Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
    Article Snippet: The membrane was incubated with Bcl-2 (1:1,000; cat. no. 4223; Cell Signaling Technology, Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature.Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature. ... The protein bands were visualized by ECL luminescent substrate (Cytiva, Amersham ImageQuant 800UV, USA), and the results were analyzed by ImageJ software.

    Article Title: Protective effect of liquiritin on coronary heart disease through regulating the proliferation of human vascular smooth muscle cells via upregulation of sirtuin1
    Article Snippet: The membrane was incubated with Bcl-2 (cat. no. 4223; 1: 1000; Cell Signaling Technology, Inc.), Bax (cat. no. 5023; 1: 1000; Cell Signaling Technology, Inc.), MMP9 (cat. no. 13,667; 1: 1000; Cell Signaling Technology, Inc.), SIRT1 (cat. no. 2496; 1: 1000; Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature.Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature. ... The protein bands were visualized using enhanced chemiluminescence luminescent substrate (Amersham ImageQuant800UV; Cytiva, MA, USA) and quantified using ImageJ software.

    Förster Resonance Energy Transfer:

    Article Title: Hierarchical small molecule inhibition of MYST acetyltransferases
    Article Snippet: ling Technology, #7003) or SuperSignal ELIZA Femto Substrate (Thermo Scientific, 37074) according to the manufacturer’s protocols. Images were captured using an Amersham ImageQuant 800 imaging system (Cytiva, 29399482). The preparation of magnetic bead H3K14-CoA affinity resin was adapted from a previously reported protocol 54 . Briefly, 15.5 mg (1 eq) of a purified peptide with the linear sequence

    Article Title: Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
    Article Snippet: The membrane was incubated with Bcl-2 (1:1,000; cat. no. 4223; Cell Signaling Technology, Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature.Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature. ... The protein bands were visualized by ECL luminescent substrate (Cytiva, Amersham ImageQuant 800UV, USA), and the results were analyzed by ImageJ software.

    Article Title: Protective effect of liquiritin on coronary heart disease through regulating the proliferation of human vascular smooth muscle cells via upregulation of sirtuin1
    Article Snippet: The membrane was incubated with Bcl-2 (cat. no. 4223; 1: 1000; Cell Signaling Technology, Inc.), Bax (cat. no. 5023; 1: 1000; Cell Signaling Technology, Inc.), MMP9 (cat. no. 13,667; 1: 1000; Cell Signaling Technology, Inc.), SIRT1 (cat. no. 2496; 1: 1000; Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature.Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature. ... The protein bands were visualized using enhanced chemiluminescence luminescent substrate (Amersham ImageQuant800UV; Cytiva, MA, USA) and quantified using ImageJ software.

    Fluorescence:

    Article Title: Hierarchical small molecule inhibition of MYST acetyltransferases
    Article Snippet: ling Technology, #7003) or SuperSignal ELIZA Femto Substrate (Thermo Scientific, 37074) according to the manufacturer’s protocols. Images were captured using an Amersham ImageQuant 800 imaging system (Cytiva, 29399482). The preparation of magnetic bead H3K14-CoA affinity resin was adapted from a previously reported protocol 54 . Briefly, 15.5 mg (1 eq) of a purified peptide with the linear sequence

    Article Title: Role of lncRNA MIAT/miR-361-3p/CCAR2 in prostate cancer cells
    Article Snippet: The membrane was incubated with Bcl-2 (1:1,000; cat. no. 4223; Cell Signaling Technology, Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature.Inc.), Bax (1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.), or GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% non-fat milk PBST solution for 1 h. After that, the membrane was incubated with the secondary antibody (cat. no. ab97080; Abcam, USA) for 1 h at room temperature. ... The protein bands were visualized by ECL luminescent substrate (Cytiva, Amersham ImageQuant 800UV, USA), and the results were analyzed by ImageJ software.

    Article Title: Protective effect of liquiritin on coronary heart disease through regulating the proliferation of human vascular smooth muscle cells via upregulation of sirtuin1
    Article Snippet: The membrane was incubated with Bcl-2 (cat. no. 4223; 1: 1000; Cell Signaling Technology, Inc.), Bax (cat. no. 5023; 1: 1000; Cell Signaling Technology, Inc.), MMP9 (cat. no. 13,667; 1: 1000; Cell Signaling Technology, Inc.), SIRT1 (cat. no. 2496; 1: 1000; Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature.Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology, Inc.) antibody at 4°C overnight after blocking with 5% nonfat milk phosphate buffer saline (PBS)-0.1%Tween 20 solution for 1 h. The membrane was then incubated with secondary antibodies (cat. no. ab96899; 1:2,000; Abcam, Cambridge, USA) for 1 h at room temperature. ... The protein bands were visualized using enhanced chemiluminescence luminescent substrate (Amersham ImageQuant800UV; Cytiva, MA, USA) and quantified using ImageJ software.



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