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imagej particle tracking plugin  (ibidi GmbH)

 
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    Structured Review

    ibidi GmbH imagej particle tracking plugin
    Imagej Particle Tracking Plugin, supplied by ibidi GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagej+plugins/imagej+particle+tracking+plugin/pm40537771-435-6-18
    Average 90 stars, based on 1 article reviews
    imagej particle tracking plugin - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Chemotaxis Assay:

    Article Title: The phosphatase PPM1F, a negative regulator of integrin activity, is essential for embryonic development and controls tumor cell invasion.
    Article Snippet: Single cells were tracked manually using ImageJ particle tracking plugin and analyzed using the chemotaxis and migration tool (Ibidi GmbH, München, Germany). .. Single cells were tracked manually using ImageJ particle tracking plugin and analyzed using the chemotaxis and migration tool (Ibidi GmbH, München, Germany). .. Cells were serum starved (DMEM + 0.5% FCS) for max 12 h. After the coating solution was removed, dishes were blocked with suspension medium (DMEM plus 0.25% BSA) and prewarmed to 37 °C for 1 h. In parallel, cells were trypsinized, cells were suspended in DMEM + soybean trypsin inhibitor (12.5 mg in 50 ml DMEM, sterile filtered; AppliChem), counted and kept in suspension medium for 45 min at 37 °C.

    Article Title: PPM1F controls integrin activity via a conserved phospho-switch
    Article Snippet: .. Single cells were tracked manually using the ImageJ particle tracking plugin and analyzed using the chemotaxis and migration tool (Ibidi GmbH). ..

    Migration:

    Article Title: The phosphatase PPM1F, a negative regulator of integrin activity, is essential for embryonic development and controls tumor cell invasion.
    Article Snippet: Single cells were tracked manually using ImageJ particle tracking plugin and analyzed using the chemotaxis and migration tool (Ibidi GmbH, München, Germany). .. Single cells were tracked manually using ImageJ particle tracking plugin and analyzed using the chemotaxis and migration tool (Ibidi GmbH, München, Germany). .. Cells were serum starved (DMEM + 0.5% FCS) for max 12 h. After the coating solution was removed, dishes were blocked with suspension medium (DMEM plus 0.25% BSA) and prewarmed to 37 °C for 1 h. In parallel, cells were trypsinized, cells were suspended in DMEM + soybean trypsin inhibitor (12.5 mg in 50 ml DMEM, sterile filtered; AppliChem), counted and kept in suspension medium for 45 min at 37 °C.

    Article Title: PPM1F controls integrin activity via a conserved phospho-switch
    Article Snippet: .. Single cells were tracked manually using the ImageJ particle tracking plugin and analyzed using the chemotaxis and migration tool (Ibidi GmbH). ..



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    Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the <t>ImageJ</t> <t>plugin</t> Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the <t>LPX</t> ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.
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    Image Search Results


    Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the ImageJ plugin Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.

    Journal: eBioMedicine

    Article Title: SLK is mutated in individuals with a neurodevelopmental disorder

    doi: 10.1016/j.ebiom.2025.105725

    Figure Lengend Snippet: Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the ImageJ plugin Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.

    Article Snippet: Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells.

    Techniques: Derivative Assay, Control, Cell Migration Assay, Migration, Software