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dedicated imagej plugin  (Nikon)


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    Structured Review

    Nikon dedicated imagej plugin
    Dedicated Imagej Plugin, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1626 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagej+plugin/ECLIPSE+Ts2R/pm41780697-118-15-8
    Average 99 stars, based on 1626 article reviews
    dedicated imagej plugin - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Morphology:

    Article Title: Development and application of zebrafish liver 3D spheroids for assessing hepatotoxicity.
    Article Snippet: With the widespread adoption of the 3R principles, three-dimensional (3D) cell models are increasingly employed in toxicology.. However, research on 3D cell models in fish remains limited.. This study established a 3D zebrafish liver (ZFL) cell model and utilized it to assess the hepatotoxicity of N-(1,3-dimethylbutyl)-N′-phenyl-pphenylenediamine quinone (6PPD-Q) and N-Isopropyl-N'-phenyl-1,4-phenylenediamine quinone (IPPD-Q), respectively.

    Article Title: Proteasome-dependent degradation of DAPK1 by wogonoside blocks PARthanatos and protects against ischemic stroke.
    Article Snippet: Ischemic stroke remains a leading cause of death and disability, yet effective pharmacological neuroprotection remains elusive.. ROS-induced PARthanatos—driven by PARP1 hyperactivation and the mitochondrial– apoptosis-inducing factor (AIF) death axis—is a principal executor of ischemic neuronal death.. However, direct PARP1 inhibition inevitably compromises essential DNA repair.

    Spheroids:

    Article Title: Development and application of zebrafish liver 3D spheroids for assessing hepatotoxicity.
    Article Snippet: With the widespread adoption of the 3R principles, three-dimensional (3D) cell models are increasingly employed in toxicology.. However, research on 3D cell models in fish remains limited.. This study established a 3D zebrafish liver (ZFL) cell model and utilized it to assess the hepatotoxicity of N-(1,3-dimethylbutyl)-N′-phenyl-pphenylenediamine quinone (6PPD-Q) and N-Isopropyl-N'-phenyl-1,4-phenylenediamine quinone (IPPD-Q), respectively.

    Inverted Microscopy:

    Article Title: Development and application of zebrafish liver 3D spheroids for assessing hepatotoxicity.
    Article Snippet: With the widespread adoption of the 3R principles, three-dimensional (3D) cell models are increasingly employed in toxicology.. However, research on 3D cell models in fish remains limited.. This study established a 3D zebrafish liver (ZFL) cell model and utilized it to assess the hepatotoxicity of N-(1,3-dimethylbutyl)-N′-phenyl-pphenylenediamine quinone (6PPD-Q) and N-Isopropyl-N'-phenyl-1,4-phenylenediamine quinone (IPPD-Q), respectively.

    Phase Contrast:

    Article Title: Proteasome-dependent degradation of DAPK1 by wogonoside blocks PARthanatos and protects against ischemic stroke.
    Article Snippet: Ischemic stroke remains a leading cause of death and disability, yet effective pharmacological neuroprotection remains elusive.. ROS-induced PARthanatos—driven by PARP1 hyperactivation and the mitochondrial– apoptosis-inducing factor (AIF) death axis—is a principal executor of ischemic neuronal death.. However, direct PARP1 inhibition inevitably compromises essential DNA repair.

    Fluorescence:

    Article Title: Proteasome-dependent degradation of DAPK1 by wogonoside blocks PARthanatos and protects against ischemic stroke.
    Article Snippet: Ischemic stroke remains a leading cause of death and disability, yet effective pharmacological neuroprotection remains elusive.. ROS-induced PARthanatos—driven by PARP1 hyperactivation and the mitochondrial– apoptosis-inducing factor (AIF) death axis—is a principal executor of ischemic neuronal death.. However, direct PARP1 inhibition inevitably compromises essential DNA repair.

    Article Title: Optimized gGBE enables robust G-to-Y conversion in mammalian cells and zebrafish embryos.
    Article Snippet: .. Fluorescence images were acquired using an inverted fluorescence imaging system (Ts2R, Nikon, Japan). .. GFP-positive cells were then sorted by flow cytometry (BD FACSAria III, USA).

    Article Title: Sex‐Specific Effects of Dexmedetomidine on Brain Microvascular Endothelial Cell Function After Traumatic Brain Injury in Juvenile Mice
    Article Snippet: Cells were then incubated with BioTracker 633 Red Mitochondria Dye (100 nM; MilliporeSigma, MA, USA) and BioTracker Mitochondrial Lipid Peroxide Live Cell Ferroptosis Indicator (100 nM; MilliporeSigma, MA, USA) for 30 min before imaging. .. Images were acquired using a Nikon Plan Apo 20×/0.9 objective mounted on a Nikon Eclipse TS2R fluorescence microscope (Nikon, NY, USA). ..

    Article Title: Prevotella histicola improves metabolic-associated fatty liver disease by promoting lipid droplet autophagy and inhibiting ER stress.
    Article Snippet: Metabolically associated fatty liver disease (MAFLD), a prevalent chronic liver disorder linked to metabolic dysfunction, is marked by hepatic lipid accumulation and inflammatory response.. Treatment options are limited, yet probiotics show potential in modulating the gut-liver axis.. Prevotella histicola (P. histicola), known for its anti-oxidant and anti-inflammatory features, provides protection against metabolic diseases, but its role in MAFLD remains unclear.

    Article Title: Integration of network toxicology and zebrafish models to elucidate bisphenol BP-induced cardiotoxicity.
    Article Snippet: Bisphenol BP (BPBP), a structural analog of bisphenol A (BPA), is increasingly utilized in industrial applications; however, its potential hazards to the cardiovascular system remain unexplored.. This study employed an integrated strategy combining network toxicology, molecular docking, and in vivo zebrafish models to systematically elucidate the cardiovascular toxicity of BPBP.. Network toxicology identified several candidate hub genes and pathways potentially associated with BPBP-induced Jo ur na l P re -p ro of Journal Pre-proof 2 cardiotoxicity.

    Article Title: Astragaloside IV improves MASLD via GCN5-LXRα/SREBP1c signaling pathway-mediated hepatic de novo lipogenesis
    Article Snippet: .. Intracellular lipid droplets were imaged for fluorescence using either a laser confocal microscope (Olympus FV3000) or an inverted fluorescence microscope (Nikon Ts2R). .. Intracellular neutral lipids were quantified using ImageJ (Version 1.5a).

    Microscopy:

    Article Title: Proteasome-dependent degradation of DAPK1 by wogonoside blocks PARthanatos and protects against ischemic stroke.
    Article Snippet: Ischemic stroke remains a leading cause of death and disability, yet effective pharmacological neuroprotection remains elusive.. ROS-induced PARthanatos—driven by PARP1 hyperactivation and the mitochondrial– apoptosis-inducing factor (AIF) death axis—is a principal executor of ischemic neuronal death.. However, direct PARP1 inhibition inevitably compromises essential DNA repair.

    Article Title: Sex‐Specific Effects of Dexmedetomidine on Brain Microvascular Endothelial Cell Function After Traumatic Brain Injury in Juvenile Mice
    Article Snippet: Cells were then incubated with BioTracker 633 Red Mitochondria Dye (100 nM; MilliporeSigma, MA, USA) and BioTracker Mitochondrial Lipid Peroxide Live Cell Ferroptosis Indicator (100 nM; MilliporeSigma, MA, USA) for 30 min before imaging. .. Images were acquired using a Nikon Plan Apo 20×/0.9 objective mounted on a Nikon Eclipse TS2R fluorescence microscope (Nikon, NY, USA). ..

    Article Title: Prevotella histicola improves metabolic-associated fatty liver disease by promoting lipid droplet autophagy and inhibiting ER stress.
    Article Snippet: Metabolically associated fatty liver disease (MAFLD), a prevalent chronic liver disorder linked to metabolic dysfunction, is marked by hepatic lipid accumulation and inflammatory response.. Treatment options are limited, yet probiotics show potential in modulating the gut-liver axis.. Prevotella histicola (P. histicola), known for its anti-oxidant and anti-inflammatory features, provides protection against metabolic diseases, but its role in MAFLD remains unclear.

    Article Title: CD73‐Overexpressing ADSCs Promote Neurogenic Bladder Repair via A2A–JAK1/STAT3‐Mediated Modulation of Inflammation and Macrophage Polarization
    Article Snippet: Tissue sections from all experimental groups were processed simultaneously with a commercial Masson staining kit (Solarbio, Beijing, China) to reduce interbatch variability. .. Representative images were captured using a Nikon Ts2R microscope (Nikon, Tokyo, Japan), and staining intensity was quantitatively analyzed with NIS‐Elements Viewer software. ..

    Article Title: Integration of network toxicology and zebrafish models to elucidate bisphenol BP-induced cardiotoxicity.
    Article Snippet: Bisphenol BP (BPBP), a structural analog of bisphenol A (BPA), is increasingly utilized in industrial applications; however, its potential hazards to the cardiovascular system remain unexplored.. This study employed an integrated strategy combining network toxicology, molecular docking, and in vivo zebrafish models to systematically elucidate the cardiovascular toxicity of BPBP.. Network toxicology identified several candidate hub genes and pathways potentially associated with BPBP-induced Jo ur na l P re -p ro of Journal Pre-proof 2 cardiotoxicity.

    Article Title: Astragaloside IV improves MASLD via GCN5-LXRα/SREBP1c signaling pathway-mediated hepatic de novo lipogenesis
    Article Snippet: .. Intracellular lipid droplets were imaged for fluorescence using either a laser confocal microscope (Olympus FV3000) or an inverted fluorescence microscope (Nikon Ts2R). .. Intracellular neutral lipids were quantified using ImageJ (Version 1.5a).

    Imaging:

    Article Title: Optimized gGBE enables robust G-to-Y conversion in mammalian cells and zebrafish embryos.
    Article Snippet: .. Fluorescence images were acquired using an inverted fluorescence imaging system (Ts2R, Nikon, Japan). .. GFP-positive cells were then sorted by flow cytometry (BD FACSAria III, USA).

    Article Title: Integration of network toxicology and zebrafish models to elucidate bisphenol BP-induced cardiotoxicity.
    Article Snippet: Bisphenol BP (BPBP), a structural analog of bisphenol A (BPA), is increasingly utilized in industrial applications; however, its potential hazards to the cardiovascular system remain unexplored.. This study employed an integrated strategy combining network toxicology, molecular docking, and in vivo zebrafish models to systematically elucidate the cardiovascular toxicity of BPBP.. Network toxicology identified several candidate hub genes and pathways potentially associated with BPBP-induced Jo ur na l P re -p ro of Journal Pre-proof 2 cardiotoxicity.

    Staining:

    Article Title: CD73‐Overexpressing ADSCs Promote Neurogenic Bladder Repair via A2A–JAK1/STAT3‐Mediated Modulation of Inflammation and Macrophage Polarization
    Article Snippet: Tissue sections from all experimental groups were processed simultaneously with a commercial Masson staining kit (Solarbio, Beijing, China) to reduce interbatch variability. .. Representative images were captured using a Nikon Ts2R microscope (Nikon, Tokyo, Japan), and staining intensity was quantitatively analyzed with NIS‐Elements Viewer software. ..

    Software:

    Article Title: CD73‐Overexpressing ADSCs Promote Neurogenic Bladder Repair via A2A–JAK1/STAT3‐Mediated Modulation of Inflammation and Macrophage Polarization
    Article Snippet: Tissue sections from all experimental groups were processed simultaneously with a commercial Masson staining kit (Solarbio, Beijing, China) to reduce interbatch variability. .. Representative images were captured using a Nikon Ts2R microscope (Nikon, Tokyo, Japan), and staining intensity was quantitatively analyzed with NIS‐Elements Viewer software. ..

    Confocal:

    Article Title: Astragaloside IV improves MASLD via GCN5-LXRα/SREBP1c signaling pathway-mediated hepatic de novo lipogenesis
    Article Snippet: .. Intracellular lipid droplets were imaged for fluorescence using either a laser confocal microscope (Olympus FV3000) or an inverted fluorescence microscope (Nikon Ts2R). .. Intracellular neutral lipids were quantified using ImageJ (Version 1.5a).



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    Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the <t>ImageJ</t> <t>plugin</t> Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the <t>LPX</t> ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.
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    Image Search Results


    Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the ImageJ plugin Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.

    Journal: eBioMedicine

    Article Title: SLK is mutated in individuals with a neurodevelopmental disorder

    doi: 10.1016/j.ebiom.2025.105725

    Figure Lengend Snippet: Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the ImageJ plugin Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.

    Article Snippet: Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells.

    Techniques: Derivative Assay, Control, Cell Migration Assay, Migration, Software