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image quant 10 2 analysis software  (Cytiva Europe)


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    Structured Review

    Cytiva Europe image quant 10 2 analysis software

    Image Quant 10 2 Analysis Software, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+quant+analysis+software/pmc11053316-73-0-6
    Average 86 stars, based on 1 article reviews
    image quant 10 2 analysis software - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Tumor invasiveness is regulated by the concerted function of APC, formins, and Arp2/3 complex"

    Article Title: Tumor invasiveness is regulated by the concerted function of APC, formins, and Arp2/3 complex

    Journal: iScience

    doi: 10.1016/j.isci.2024.109687


    Figure Legend Snippet:

    Techniques Used: Recombinant, Modification, Saline, Western Blot, Plasmid Preparation, Software, Microscopy, Chemotaxis Assay, Migration

    Related Articles

    Western Blot:

    Article Title: Tumor invasiveness is regulated by the concerted function of APC, formins, and Arp2/3 complex
    Article Snippet: The blots were then incubated with 1:3000 secondary antibodies either anti-rabbit (A0545) or anti-mouse (A9044) IgG-horseradish peroxidase-conjugated (Sigma-Aldrich) diluted in TBS-T for 1 h at room temperature. .. The immune complexes were detected using ECL prime western blotting detection kit and a Uvitec Q9 alliance imaging system coupled to an Image Quant 10.2 software (Cytiva). .. To determine the APC protein levels in LS174T colorectal cancer cell lines stably expressing APC-WT or APC-m4, cells were seeded on 18 mm 2 round coverslips (#631-0153; VWR), fixed with warm 4% paraformaldehyde (#28908, Thermo Scientific) in Phosphate-Buffered Saline (PBS: 2.7 mM KCl, 1.8 mM KH 2 PO 4 , 10 mM Na 2 HPO 4 , 140 mM NaCl, pH 7.4) (#P4417 Sigma-Aldrich) for 15 minutes, washed once with PBS, permeabilized with 0.1% Triton X-100 (catalogue# T8787-100ML, Sigma-Aldrich) for 10 minutes, washed once with 1x PBST (1× PBS, 0.1% v/v Tween-20 : catalogue# P1379-100ML; Sigma-Aldrich), blocked with 3% BSA (#A3059-10G; Sigma-Aldrich) for 1 hour at room temperature and incubated overnight at 4°C with anti-APC C-terminus (1:250, Abcam, ab154906, RRID: AB_2861396 ).

    Imaging:

    Article Title: Tumor invasiveness is regulated by the concerted function of APC, formins, and Arp2/3 complex
    Article Snippet: The blots were then incubated with 1:3000 secondary antibodies either anti-rabbit (A0545) or anti-mouse (A9044) IgG-horseradish peroxidase-conjugated (Sigma-Aldrich) diluted in TBS-T for 1 h at room temperature. .. The immune complexes were detected using ECL prime western blotting detection kit and a Uvitec Q9 alliance imaging system coupled to an Image Quant 10.2 software (Cytiva). .. To determine the APC protein levels in LS174T colorectal cancer cell lines stably expressing APC-WT or APC-m4, cells were seeded on 18 mm 2 round coverslips (#631-0153; VWR), fixed with warm 4% paraformaldehyde (#28908, Thermo Scientific) in Phosphate-Buffered Saline (PBS: 2.7 mM KCl, 1.8 mM KH 2 PO 4 , 10 mM Na 2 HPO 4 , 140 mM NaCl, pH 7.4) (#P4417 Sigma-Aldrich) for 15 minutes, washed once with PBS, permeabilized with 0.1% Triton X-100 (catalogue# T8787-100ML, Sigma-Aldrich) for 10 minutes, washed once with 1x PBST (1× PBS, 0.1% v/v Tween-20 : catalogue# P1379-100ML; Sigma-Aldrich), blocked with 3% BSA (#A3059-10G; Sigma-Aldrich) for 1 hour at room temperature and incubated overnight at 4°C with anti-APC C-terminus (1:250, Abcam, ab154906, RRID: AB_2861396 ).

    Software:

    Article Title: Tumor invasiveness is regulated by the concerted function of APC, formins, and Arp2/3 complex
    Article Snippet: The blots were then incubated with 1:3000 secondary antibodies either anti-rabbit (A0545) or anti-mouse (A9044) IgG-horseradish peroxidase-conjugated (Sigma-Aldrich) diluted in TBS-T for 1 h at room temperature. .. The immune complexes were detected using ECL prime western blotting detection kit and a Uvitec Q9 alliance imaging system coupled to an Image Quant 10.2 software (Cytiva). .. To determine the APC protein levels in LS174T colorectal cancer cell lines stably expressing APC-WT or APC-m4, cells were seeded on 18 mm 2 round coverslips (#631-0153; VWR), fixed with warm 4% paraformaldehyde (#28908, Thermo Scientific) in Phosphate-Buffered Saline (PBS: 2.7 mM KCl, 1.8 mM KH 2 PO 4 , 10 mM Na 2 HPO 4 , 140 mM NaCl, pH 7.4) (#P4417 Sigma-Aldrich) for 15 minutes, washed once with PBS, permeabilized with 0.1% Triton X-100 (catalogue# T8787-100ML, Sigma-Aldrich) for 10 minutes, washed once with 1x PBST (1× PBS, 0.1% v/v Tween-20 : catalogue# P1379-100ML; Sigma-Aldrich), blocked with 3% BSA (#A3059-10G; Sigma-Aldrich) for 1 hour at room temperature and incubated overnight at 4°C with anti-APC C-terminus (1:250, Abcam, ab154906, RRID: AB_2861396 ).

    Article Title: Tumor invasiveness is regulated by the concerted function of APC, formins, and Arp2/3 complex
    Article Snippet: Fiji / ImageJ version 2.3 , NIH – public domain , RRID: SCR_002285. .. Image Quant 10.2 analysis software , Cytiva , RRID: SCR_014246. ..



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    Image Search Results


    Journal: iScience

    Article Title: Tumor invasiveness is regulated by the concerted function of APC, formins, and Arp2/3 complex

    doi: 10.1016/j.isci.2024.109687

    Figure Lengend Snippet:

    Article Snippet: Image Quant 10.2 analysis software , Cytiva , RRID: SCR_014246.

    Techniques: Recombinant, Modification, Saline, Western Blot, Plasmid Preparation, Software, Microscopy, Chemotaxis Assay, Migration