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image based morphometric analysis  (Nikon)


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    Nikon image based morphometric analysis
    Image Based Morphometric Analysis, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+morphometric+analysis/NIS-Elements/pmc11224734-58-6-12
    Average 99 stars, based on 39904 article reviews
    image based morphometric analysis - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Confocal:

    Article Title: Functional interactions between anteroventral periventricular kisspeptin neurons and gonadotropin‐releasing hormone neurons in female mice
    Article Snippet: Regional images were obtained on an Olympus IX81 using cellSens software (Evident Scientific). .. Confocal images were obtained on a Nikon A1 inverted confocal using NIS‐Elements software (version 6.20.02, Nikon Metrology). ..

    Article Title: Co-expression of non-signaling CARs enhances EGFRvIII-specific CAR T-cell cytotoxicity against head and neck squamous cell carcinoma.
    Article Snippet: Stained CAR/nsCAR T-cells were subsequently co-cultured with the target cells and analyzed by fluorescence microscopy using an IX83 microscope system (Olympus, Japan) equipped with a 60× oil-immersion objective (NA 0.65–1.25) and CellSens Dimensions software (Olympus, version 1.18). .. Additional confocal imaging was performed using Nikon ECLIPSE Ti2 microscope (Nikon, Japan), equipped with a 100× oil-immersion objective (NA 1.49) and NIS-Elements AR-SP software (Nikon). ..

    Article Title: Nitrate-responsive Sialin2 couples stress granule assembly and clearance to maintain neuronal homeostasis.
    Article Snippet: Article Nitrate-responsive Sialin2 couples stress granule assembly and clearance to maintain neuronal homeostasis

    Article Title: An extramedullary bone model of metastatic dormancy reveals an increased rate of metastatic reactivation upon distant insults.
    Article Snippet: .. Images were acquired using a Zeiss Upright710 confocal microscope with NIS-elements software (version 4.51, Nikon, Japan) or a Zeiss Axio Scan.Z1 slide scanner, and analysis performed with Fiji (v. 2.9.0/1.53t, ImageJ). .. For quantification of GFP-positive bone cell percentages in each lineage (Data S3), slides were stained by immunofluorescence as detailed above, and subsequently scanned with Zeiss Axio Scan.Z1 at 20× objective.

    Software:

    Article Title: Functional interactions between anteroventral periventricular kisspeptin neurons and gonadotropin‐releasing hormone neurons in female mice
    Article Snippet: Regional images were obtained on an Olympus IX81 using cellSens software (Evident Scientific). .. Confocal images were obtained on a Nikon A1 inverted confocal using NIS‐Elements software (version 6.20.02, Nikon Metrology). ..

    Article Title: Co-expression of non-signaling CARs enhances EGFRvIII-specific CAR T-cell cytotoxicity against head and neck squamous cell carcinoma.
    Article Snippet: Stained CAR/nsCAR T-cells were subsequently co-cultured with the target cells and analyzed by fluorescence microscopy using an IX83 microscope system (Olympus, Japan) equipped with a 60× oil-immersion objective (NA 0.65–1.25) and CellSens Dimensions software (Olympus, version 1.18). .. Additional confocal imaging was performed using Nikon ECLIPSE Ti2 microscope (Nikon, Japan), equipped with a 100× oil-immersion objective (NA 1.49) and NIS-Elements AR-SP software (Nikon). ..

    Article Title: Nitrate-responsive Sialin2 couples stress granule assembly and clearance to maintain neuronal homeostasis.
    Article Snippet: Article Nitrate-responsive Sialin2 couples stress granule assembly and clearance to maintain neuronal homeostasis

    Article Title: Evaluation of Biological Effect of Triticum vulgare Extract Compared With Hyaluronic Acid in In Vitro Tissue Models
    Article Snippet: .. Images were acquired using a Nikon fluorescence microscope and analyzed using Nikon Nis‐Elements software (Version D.4.00). ..

    Article Title: Quantitative comparison of PI(3,5)P 2 biosensors reveals SnxA is the most sensitive and unbiased
    Article Snippet: .. In some experiments, the Nikon Elements denoising software was also used. ..

    Article Title: Stabilization of microbial cell population for long-term plasmid production in continuous biomanufacturing.
    Article Snippet: 1 Terra Research and Teaching Center, Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium 2 GSK, Siena, Italy 3 GSK, Rixensart, Belgium Abstract Bacterial cells such as Escherichia coli are generally considered insensitive to shear stress in stirred bioreactors, primarily because their small size is assumed to place them below the length scales at which mechanical forces become damaging.. This assumption underlies the widespread use of high agitation rates in microbial bioprocesses.. However, cellular physiology can strongly modulate this apparent robustness.

    Article Title: An extramedullary bone model of metastatic dormancy reveals an increased rate of metastatic reactivation upon distant insults.
    Article Snippet: .. Images were acquired using a Zeiss Upright710 confocal microscope with NIS-elements software (version 4.51, Nikon, Japan) or a Zeiss Axio Scan.Z1 slide scanner, and analysis performed with Fiji (v. 2.9.0/1.53t, ImageJ). .. For quantification of GFP-positive bone cell percentages in each lineage (Data S3), slides were stained by immunofluorescence as detailed above, and subsequently scanned with Zeiss Axio Scan.Z1 at 20× objective.

    Article Title: Adolescent Ethanol Exposure Disrupts the Mature Phenotype of Surviving Neuroprogenitors in Female Mice.
    Article Snippet: .. Number of putative varicosities in the polymorphic (hilar) and CA3 regions of the hippocampus was assessed using Nikon NIS- Elements software. ..

    Imaging:

    Article Title: Co-expression of non-signaling CARs enhances EGFRvIII-specific CAR T-cell cytotoxicity against head and neck squamous cell carcinoma.
    Article Snippet: Stained CAR/nsCAR T-cells were subsequently co-cultured with the target cells and analyzed by fluorescence microscopy using an IX83 microscope system (Olympus, Japan) equipped with a 60× oil-immersion objective (NA 0.65–1.25) and CellSens Dimensions software (Olympus, version 1.18). .. Additional confocal imaging was performed using Nikon ECLIPSE Ti2 microscope (Nikon, Japan), equipped with a 100× oil-immersion objective (NA 1.49) and NIS-Elements AR-SP software (Nikon). ..

    Article Title: Stabilization of microbial cell population for long-term plasmid production in continuous biomanufacturing.
    Article Snippet: 1 Terra Research and Teaching Center, Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium 2 GSK, Siena, Italy 3 GSK, Rixensart, Belgium Abstract Bacterial cells such as Escherichia coli are generally considered insensitive to shear stress in stirred bioreactors, primarily because their small size is assumed to place them below the length scales at which mechanical forces become damaging.. This assumption underlies the widespread use of high agitation rates in microbial bioprocesses.. However, cellular physiology can strongly modulate this apparent robustness.

    Microscopy:

    Article Title: Co-expression of non-signaling CARs enhances EGFRvIII-specific CAR T-cell cytotoxicity against head and neck squamous cell carcinoma.
    Article Snippet: Stained CAR/nsCAR T-cells were subsequently co-cultured with the target cells and analyzed by fluorescence microscopy using an IX83 microscope system (Olympus, Japan) equipped with a 60× oil-immersion objective (NA 0.65–1.25) and CellSens Dimensions software (Olympus, version 1.18). .. Additional confocal imaging was performed using Nikon ECLIPSE Ti2 microscope (Nikon, Japan), equipped with a 100× oil-immersion objective (NA 1.49) and NIS-Elements AR-SP software (Nikon). ..

    Article Title: Nitrate-responsive Sialin2 couples stress granule assembly and clearance to maintain neuronal homeostasis.
    Article Snippet: Article Nitrate-responsive Sialin2 couples stress granule assembly and clearance to maintain neuronal homeostasis

    Article Title: Evaluation of Biological Effect of Triticum vulgare Extract Compared With Hyaluronic Acid in In Vitro Tissue Models
    Article Snippet: .. Images were acquired using a Nikon fluorescence microscope and analyzed using Nikon Nis‐Elements software (Version D.4.00). ..

    Article Title: An extramedullary bone model of metastatic dormancy reveals an increased rate of metastatic reactivation upon distant insults.
    Article Snippet: .. Images were acquired using a Zeiss Upright710 confocal microscope with NIS-elements software (version 4.51, Nikon, Japan) or a Zeiss Axio Scan.Z1 slide scanner, and analysis performed with Fiji (v. 2.9.0/1.53t, ImageJ). .. For quantification of GFP-positive bone cell percentages in each lineage (Data S3), slides were stained by immunofluorescence as detailed above, and subsequently scanned with Zeiss Axio Scan.Z1 at 20× objective.

    Fluorescence:

    Article Title: Evaluation of Biological Effect of Triticum vulgare Extract Compared With Hyaluronic Acid in In Vitro Tissue Models
    Article Snippet: .. Images were acquired using a Nikon fluorescence microscope and analyzed using Nikon Nis‐Elements software (Version D.4.00). ..

    Timelapse:

    Article Title: Stabilization of microbial cell population for long-term plasmid production in continuous biomanufacturing.
    Article Snippet: 1 Terra Research and Teaching Center, Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium 2 GSK, Siena, Italy 3 GSK, Rixensart, Belgium Abstract Bacterial cells such as Escherichia coli are generally considered insensitive to shear stress in stirred bioreactors, primarily because their small size is assumed to place them below the length scales at which mechanical forces become damaging.. This assumption underlies the widespread use of high agitation rates in microbial bioprocesses.. However, cellular physiology can strongly modulate this apparent robustness.

    Analysis:

    Article Title: An extramedullary bone model of metastatic dormancy reveals an increased rate of metastatic reactivation upon distant insults.
    Article Snippet: .. Images were acquired using a Zeiss Upright710 confocal microscope with NIS-elements software (version 4.51, Nikon, Japan) or a Zeiss Axio Scan.Z1 slide scanner, and analysis performed with Fiji (v. 2.9.0/1.53t, ImageJ). .. For quantification of GFP-positive bone cell percentages in each lineage (Data S3), slides were stained by immunofluorescence as detailed above, and subsequently scanned with Zeiss Axio Scan.Z1 at 20× objective.



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    Third harmonic generation (THG) brain imaging in groups of Danionella dracula 3 to 90 dpf. A . Left: Side view of a male. Scale bar indicates 3mm. Right: Head-on view of male extending its hypertrophied lower jaw during mid-lunge at another male. B . Dorsal views of fish imaged from 90-3 dpf (left to right). Scale bar left indicates 500 μm for all images. Top insert at 90 dpf is zoom-in on fangs (indicated by arrows; dashed outline on left fang), which are first apparent in 90 dpf males along with hypertrophied lower jaw. C . Schematic of imaging apparatus (see Materials and Methods). Left: profile drawing of animal placement under objective. Right: dorsal view of adult placed in putty holder with perfusion mouthpiece. Fish imaged less than 60 dpf were imaged using an alternative setup than pictured (see Materials and Methods, Fish stabilization for imaging). D. 30, 21, and 14 dpf example fish depicting changes in swim bladder (SB) separation into anterior and posterior chambers. Scale bar indicates 500 μm for all images. E . Schematic of representative D. dracula brain; illustrations based on pictures of dissected whole brains. Brain regions of <t>morphometric</t> analysis indicated in lateral and dorsal views; green, OB (olfactory bulb); blue, telencephalon; orange, TL (torus longitudinalis); pink, TeO (optic tectum). Insert indicates horizontal slice in midbrain depicting TeO (pink), periventricular gray zone (PGZ) of TeO (purple, included in TeO measurement), depicted for indication of boundary between TeO and TS (yellow, torus semicircularis). F . Scatterplot of age in dpf of animals in this study and gross brain volume in μm 3 . Boxplots indicate median, upper and lower quartiles and 1.5× interquartile range for each Age. Gross brain volume calculation is described in and Materials and Methods. Different ages depicted by different colored dots as shown in legend: red, 5 dpf; yellow, 14 dpf; green, 21 dpf; teal, 30 dpf; blue, 60 dpf; purple, 90 dpf. As is described in Materials and Methods, we performed the best fitting quadratic regression (F 2,30= 120.8, R 2 = 0.8821, p<0.001) on log-transformed data to normalize the residuals. The green line displayed here represents the quadratic regression line based on the original (non-transformed) data, for best visual clarity and understanding.
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    Third harmonic generation (THG) brain imaging in groups of Danionella dracula 3 to 90 dpf. A . Left: Side view of a male. Scale bar indicates 3mm. Right: Head-on view of male extending its hypertrophied lower jaw during mid-lunge at another male. B . Dorsal views of fish imaged from 90-3 dpf (left to right). Scale bar left indicates 500 μm for all images. Top insert at 90 dpf is zoom-in on fangs (indicated by arrows; dashed outline on left fang), which are first apparent in 90 dpf males along with hypertrophied lower jaw. C . Schematic of imaging apparatus (see Materials and Methods). Left: profile drawing of animal placement under objective. Right: dorsal view of adult placed in putty holder with perfusion mouthpiece. Fish imaged less than 60 dpf were imaged using an alternative setup than pictured (see Materials and Methods, Fish stabilization for imaging). D. 30, 21, and 14 dpf example fish depicting changes in swim bladder (SB) separation into anterior and posterior chambers. Scale bar indicates 500 μm for all images. E . Schematic of representative D. dracula brain; illustrations based on pictures of dissected whole brains. Brain regions of <t>morphometric</t> analysis indicated in lateral and dorsal views; green, OB (olfactory bulb); blue, telencephalon; orange, TL (torus longitudinalis); pink, TeO (optic tectum). Insert indicates horizontal slice in midbrain depicting TeO (pink), periventricular gray zone (PGZ) of TeO (purple, included in TeO measurement), depicted for indication of boundary between TeO and TS (yellow, torus semicircularis). F . Scatterplot of age in dpf of animals in this study and gross brain volume in μm 3 . Boxplots indicate median, upper and lower quartiles and 1.5× interquartile range for each Age. Gross brain volume calculation is described in and Materials and Methods. Different ages depicted by different colored dots as shown in legend: red, 5 dpf; yellow, 14 dpf; green, 21 dpf; teal, 30 dpf; blue, 60 dpf; purple, 90 dpf. As is described in Materials and Methods, we performed the best fitting quadratic regression (F 2,30= 120.8, R 2 = 0.8821, p<0.001) on log-transformed data to normalize the residuals. The green line displayed here represents the quadratic regression line based on the original (non-transformed) data, for best visual clarity and understanding.
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    Third harmonic generation (THG) brain imaging in groups of Danionella dracula 3 to 90 dpf. A . Left: Side view of a male. Scale bar indicates 3mm. Right: Head-on view of male extending its hypertrophied lower jaw during mid-lunge at another male. B . Dorsal views of fish imaged from 90-3 dpf (left to right). Scale bar left indicates 500 μm for all images. Top insert at 90 dpf is zoom-in on fangs (indicated by arrows; dashed outline on left fang), which are first apparent in 90 dpf males along with hypertrophied lower jaw. C . Schematic of imaging apparatus (see Materials and Methods). Left: profile drawing of animal placement under objective. Right: dorsal view of adult placed in putty holder with perfusion mouthpiece. Fish imaged less than 60 dpf were imaged using an alternative setup than pictured (see Materials and Methods, Fish stabilization for imaging). D. 30, 21, and 14 dpf example fish depicting changes in swim bladder (SB) separation into anterior and posterior chambers. Scale bar indicates 500 μm for all images. E . Schematic of representative D. dracula brain; illustrations based on pictures of dissected whole brains. Brain regions of <t>morphometric</t> analysis indicated in lateral and dorsal views; green, OB (olfactory bulb); blue, telencephalon; orange, TL (torus longitudinalis); pink, TeO (optic tectum). Insert indicates horizontal slice in midbrain depicting TeO (pink), periventricular gray zone (PGZ) of TeO (purple, included in TeO measurement), depicted for indication of boundary between TeO and TS (yellow, torus semicircularis). F . Scatterplot of age in dpf of animals in this study and gross brain volume in μm 3 . Boxplots indicate median, upper and lower quartiles and 1.5× interquartile range for each Age. Gross brain volume calculation is described in and Materials and Methods. Different ages depicted by different colored dots as shown in legend: red, 5 dpf; yellow, 14 dpf; green, 21 dpf; teal, 30 dpf; blue, 60 dpf; purple, 90 dpf. As is described in Materials and Methods, we performed the best fitting quadratic regression (F 2,30= 120.8, R 2 = 0.8821, p<0.001) on log-transformed data to normalize the residuals. The green line displayed here represents the quadratic regression line based on the original (non-transformed) data, for best visual clarity and understanding.
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    Third harmonic generation (THG) brain imaging in groups of Danionella dracula 3 to 90 dpf. A . Left: Side view of a male. Scale bar indicates 3mm. Right: Head-on view of male extending its hypertrophied lower jaw during mid-lunge at another male. B . Dorsal views of fish imaged from 90-3 dpf (left to right). Scale bar left indicates 500 μm for all images. Top insert at 90 dpf is zoom-in on fangs (indicated by arrows; dashed outline on left fang), which are first apparent in 90 dpf males along with hypertrophied lower jaw. C . Schematic of imaging apparatus (see Materials and Methods). Left: profile drawing of animal placement under objective. Right: dorsal view of adult placed in putty holder with perfusion mouthpiece. Fish imaged less than 60 dpf were imaged using an alternative setup than pictured (see Materials and Methods, Fish stabilization for imaging). D. 30, 21, and 14 dpf example fish depicting changes in swim bladder (SB) separation into anterior and posterior chambers. Scale bar indicates 500 μm for all images. E . Schematic of representative D. dracula brain; illustrations based on pictures of dissected whole brains. Brain regions of <t>morphometric</t> analysis indicated in lateral and dorsal views; green, OB (olfactory bulb); blue, telencephalon; orange, TL (torus longitudinalis); pink, TeO (optic tectum). Insert indicates horizontal slice in midbrain depicting TeO (pink), periventricular gray zone (PGZ) of TeO (purple, included in TeO measurement), depicted for indication of boundary between TeO and TS (yellow, torus semicircularis). F . Scatterplot of age in dpf of animals in this study and gross brain volume in μm 3 . Boxplots indicate median, upper and lower quartiles and 1.5× interquartile range for each Age. Gross brain volume calculation is described in and Materials and Methods. Different ages depicted by different colored dots as shown in legend: red, 5 dpf; yellow, 14 dpf; green, 21 dpf; teal, 30 dpf; blue, 60 dpf; purple, 90 dpf. As is described in Materials and Methods, we performed the best fitting quadratic regression (F 2,30= 120.8, R 2 = 0.8821, p<0.001) on log-transformed data to normalize the residuals. The green line displayed here represents the quadratic regression line based on the original (non-transformed) data, for best visual clarity and understanding.
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    Third harmonic generation (THG) brain imaging in groups of Danionella dracula 3 to 90 dpf. A . Left: Side view of a male. Scale bar indicates 3mm. Right: Head-on view of male extending its hypertrophied lower jaw during mid-lunge at another male. B . Dorsal views of fish imaged from 90-3 dpf (left to right). Scale bar left indicates 500 μm for all images. Top insert at 90 dpf is zoom-in on fangs (indicated by arrows; dashed outline on left fang), which are first apparent in 90 dpf males along with hypertrophied lower jaw. C . Schematic of imaging apparatus (see Materials and Methods). Left: profile drawing of animal placement under objective. Right: dorsal view of adult placed in putty holder with perfusion mouthpiece. Fish imaged less than 60 dpf were imaged using an alternative setup than pictured (see Materials and Methods, Fish stabilization for imaging). D. 30, 21, and 14 dpf example fish depicting changes in swim bladder (SB) separation into anterior and posterior chambers. Scale bar indicates 500 μm for all images. E . Schematic of representative D. dracula brain; illustrations based on pictures of dissected whole brains. Brain regions of <t>morphometric</t> analysis indicated in lateral and dorsal views; green, OB (olfactory bulb); blue, telencephalon; orange, TL (torus longitudinalis); pink, TeO (optic tectum). Insert indicates horizontal slice in midbrain depicting TeO (pink), periventricular gray zone (PGZ) of TeO (purple, included in TeO measurement), depicted for indication of boundary between TeO and TS (yellow, torus semicircularis). F . Scatterplot of age in dpf of animals in this study and gross brain volume in μm 3 . Boxplots indicate median, upper and lower quartiles and 1.5× interquartile range for each Age. Gross brain volume calculation is described in and Materials and Methods. Different ages depicted by different colored dots as shown in legend: red, 5 dpf; yellow, 14 dpf; green, 21 dpf; teal, 30 dpf; blue, 60 dpf; purple, 90 dpf. As is described in Materials and Methods, we performed the best fitting quadratic regression (F 2,30= 120.8, R 2 = 0.8821, p<0.001) on log-transformed data to normalize the residuals. The green line displayed here represents the quadratic regression line based on the original (non-transformed) data, for best visual clarity and understanding.
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    Third harmonic generation (THG) brain imaging in groups of Danionella dracula 3 to 90 dpf. A . Left: Side view of a male. Scale bar indicates 3mm. Right: Head-on view of male extending its hypertrophied lower jaw during mid-lunge at another male. B . Dorsal views of fish imaged from 90-3 dpf (left to right). Scale bar left indicates 500 μm for all images. Top insert at 90 dpf is zoom-in on fangs (indicated by arrows; dashed outline on left fang), which are first apparent in 90 dpf males along with hypertrophied lower jaw. C . Schematic of imaging apparatus (see Materials and Methods). Left: profile drawing of animal placement under objective. Right: dorsal view of adult placed in putty holder with perfusion mouthpiece. Fish imaged less than 60 dpf were imaged using an alternative setup than pictured (see Materials and Methods, Fish stabilization for imaging). D. 30, 21, and 14 dpf example fish depicting changes in swim bladder (SB) separation into anterior and posterior chambers. Scale bar indicates 500 μm for all images. E . Schematic of representative D. dracula brain; illustrations based on pictures of dissected whole brains. Brain regions of <t>morphometric</t> analysis indicated in lateral and dorsal views; green, OB (olfactory bulb); blue, telencephalon; orange, TL (torus longitudinalis); pink, TeO (optic tectum). Insert indicates horizontal slice in midbrain depicting TeO (pink), periventricular gray zone (PGZ) of TeO (purple, included in TeO measurement), depicted for indication of boundary between TeO and TS (yellow, torus semicircularis). F . Scatterplot of age in dpf of animals in this study and gross brain volume in μm 3 . Boxplots indicate median, upper and lower quartiles and 1.5× interquartile range for each Age. Gross brain volume calculation is described in and Materials and Methods. Different ages depicted by different colored dots as shown in legend: red, 5 dpf; yellow, 14 dpf; green, 21 dpf; teal, 30 dpf; blue, 60 dpf; purple, 90 dpf. As is described in Materials and Methods, we performed the best fitting quadratic regression (F 2,30= 120.8, R 2 = 0.8821, p<0.001) on log-transformed data to normalize the residuals. The green line displayed here represents the quadratic regression line based on the original (non-transformed) data, for best visual clarity and understanding.
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    universal imaging inc computer-based morphometric analysis image 1
    Third harmonic generation (THG) brain imaging in groups of Danionella dracula 3 to 90 dpf. A . Left: Side view of a male. Scale bar indicates 3mm. Right: Head-on view of male extending its hypertrophied lower jaw during mid-lunge at another male. B . Dorsal views of fish imaged from 90-3 dpf (left to right). Scale bar left indicates 500 μm for all images. Top insert at 90 dpf is zoom-in on fangs (indicated by arrows; dashed outline on left fang), which are first apparent in 90 dpf males along with hypertrophied lower jaw. C . Schematic of imaging apparatus (see Materials and Methods). Left: profile drawing of animal placement under objective. Right: dorsal view of adult placed in putty holder with perfusion mouthpiece. Fish imaged less than 60 dpf were imaged using an alternative setup than pictured (see Materials and Methods, Fish stabilization for imaging). D. 30, 21, and 14 dpf example fish depicting changes in swim bladder (SB) separation into anterior and posterior chambers. Scale bar indicates 500 μm for all images. E . Schematic of representative D. dracula brain; illustrations based on pictures of dissected whole brains. Brain regions of <t>morphometric</t> analysis indicated in lateral and dorsal views; green, OB (olfactory bulb); blue, telencephalon; orange, TL (torus longitudinalis); pink, TeO (optic tectum). Insert indicates horizontal slice in midbrain depicting TeO (pink), periventricular gray zone (PGZ) of TeO (purple, included in TeO measurement), depicted for indication of boundary between TeO and TS (yellow, torus semicircularis). F . Scatterplot of age in dpf of animals in this study and gross brain volume in μm 3 . Boxplots indicate median, upper and lower quartiles and 1.5× interquartile range for each Age. Gross brain volume calculation is described in and Materials and Methods. Different ages depicted by different colored dots as shown in legend: red, 5 dpf; yellow, 14 dpf; green, 21 dpf; teal, 30 dpf; blue, 60 dpf; purple, 90 dpf. As is described in Materials and Methods, we performed the best fitting quadratic regression (F 2,30= 120.8, R 2 = 0.8821, p<0.001) on log-transformed data to normalize the residuals. The green line displayed here represents the quadratic regression line based on the original (non-transformed) data, for best visual clarity and understanding.
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    Third harmonic generation (THG) brain imaging in groups of Danionella dracula 3 to 90 dpf. A . Left: Side view of a male. Scale bar indicates 3mm. Right: Head-on view of male extending its hypertrophied lower jaw during mid-lunge at another male. B . Dorsal views of fish imaged from 90-3 dpf (left to right). Scale bar left indicates 500 μm for all images. Top insert at 90 dpf is zoom-in on fangs (indicated by arrows; dashed outline on left fang), which are first apparent in 90 dpf males along with hypertrophied lower jaw. C . Schematic of imaging apparatus (see Materials and Methods). Left: profile drawing of animal placement under objective. Right: dorsal view of adult placed in putty holder with perfusion mouthpiece. Fish imaged less than 60 dpf were imaged using an alternative setup than pictured (see Materials and Methods, Fish stabilization for imaging). D. 30, 21, and 14 dpf example fish depicting changes in swim bladder (SB) separation into anterior and posterior chambers. Scale bar indicates 500 μm for all images. E . Schematic of representative D. dracula brain; illustrations based on pictures of dissected whole brains. Brain regions of morphometric analysis indicated in lateral and dorsal views; green, OB (olfactory bulb); blue, telencephalon; orange, TL (torus longitudinalis); pink, TeO (optic tectum). Insert indicates horizontal slice in midbrain depicting TeO (pink), periventricular gray zone (PGZ) of TeO (purple, included in TeO measurement), depicted for indication of boundary between TeO and TS (yellow, torus semicircularis). F . Scatterplot of age in dpf of animals in this study and gross brain volume in μm 3 . Boxplots indicate median, upper and lower quartiles and 1.5× interquartile range for each Age. Gross brain volume calculation is described in and Materials and Methods. Different ages depicted by different colored dots as shown in legend: red, 5 dpf; yellow, 14 dpf; green, 21 dpf; teal, 30 dpf; blue, 60 dpf; purple, 90 dpf. As is described in Materials and Methods, we performed the best fitting quadratic regression (F 2,30= 120.8, R 2 = 0.8821, p<0.001) on log-transformed data to normalize the residuals. The green line displayed here represents the quadratic regression line based on the original (non-transformed) data, for best visual clarity and understanding.

    Journal: bioRxiv

    Article Title: Label-free multiphoton imaging reveals volumetric shifts across development in sensory-related brain regions of a miniature transparent vertebrate

    doi: 10.1101/2024.07.18.604134

    Figure Lengend Snippet: Third harmonic generation (THG) brain imaging in groups of Danionella dracula 3 to 90 dpf. A . Left: Side view of a male. Scale bar indicates 3mm. Right: Head-on view of male extending its hypertrophied lower jaw during mid-lunge at another male. B . Dorsal views of fish imaged from 90-3 dpf (left to right). Scale bar left indicates 500 μm for all images. Top insert at 90 dpf is zoom-in on fangs (indicated by arrows; dashed outline on left fang), which are first apparent in 90 dpf males along with hypertrophied lower jaw. C . Schematic of imaging apparatus (see Materials and Methods). Left: profile drawing of animal placement under objective. Right: dorsal view of adult placed in putty holder with perfusion mouthpiece. Fish imaged less than 60 dpf were imaged using an alternative setup than pictured (see Materials and Methods, Fish stabilization for imaging). D. 30, 21, and 14 dpf example fish depicting changes in swim bladder (SB) separation into anterior and posterior chambers. Scale bar indicates 500 μm for all images. E . Schematic of representative D. dracula brain; illustrations based on pictures of dissected whole brains. Brain regions of morphometric analysis indicated in lateral and dorsal views; green, OB (olfactory bulb); blue, telencephalon; orange, TL (torus longitudinalis); pink, TeO (optic tectum). Insert indicates horizontal slice in midbrain depicting TeO (pink), periventricular gray zone (PGZ) of TeO (purple, included in TeO measurement), depicted for indication of boundary between TeO and TS (yellow, torus semicircularis). F . Scatterplot of age in dpf of animals in this study and gross brain volume in μm 3 . Boxplots indicate median, upper and lower quartiles and 1.5× interquartile range for each Age. Gross brain volume calculation is described in and Materials and Methods. Different ages depicted by different colored dots as shown in legend: red, 5 dpf; yellow, 14 dpf; green, 21 dpf; teal, 30 dpf; blue, 60 dpf; purple, 90 dpf. As is described in Materials and Methods, we performed the best fitting quadratic regression (F 2,30= 120.8, R 2 = 0.8821, p<0.001) on log-transformed data to normalize the residuals. The green line displayed here represents the quadratic regression line based on the original (non-transformed) data, for best visual clarity and understanding.

    Article Snippet: Morphometric image analysis was performed using the software Imaris (BITPLANE, Oxford Instruments).

    Techniques: Imaging, Transformation Assay