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computer-assisted morphometric analysis of hepatic histopathology  (universal imaging inc)

 
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    universal imaging inc computer-assisted morphometric analysis of hepatic histopathology
    Computer Assisted Morphometric Analysis Of Hepatic Histopathology, supplied by universal imaging inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+morphometric+analysis/computer+assisted+morphometric+analysis+of+hepatic+histopathology/pmc04561589-80-22-38
    Average 90 stars, based on 1 article reviews
    computer-assisted morphometric analysis of hepatic histopathology - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Clinical Proteomics:

    Article Title: Successful Within-patient Dose Escalation of Olipudase Alfa in Acid Sphingomyelinase Deficiency
    Article Snippet: Pharmacodynamic parameters included measurement of plasma sphingomyelin, and ceramide levels by liquid chromatography-tandem mass spectrometry (LC/MS/MS) and liver sphingomyelin content by both LC/MS/MS and computer-assisted morphometric analysis of hepatic histopathology (MetaMorph Imaging Processing and Analysis software (Version 6.3; Universal Imaging Corporation) ( 11 ).

    Chromatography:

    Article Title: Successful Within-patient Dose Escalation of Olipudase Alfa in Acid Sphingomyelinase Deficiency
    Article Snippet: Pharmacodynamic parameters included measurement of plasma sphingomyelin, and ceramide levels by liquid chromatography-tandem mass spectrometry (LC/MS/MS) and liver sphingomyelin content by both LC/MS/MS and computer-assisted morphometric analysis of hepatic histopathology (MetaMorph Imaging Processing and Analysis software (Version 6.3; Universal Imaging Corporation) ( 11 ).

    Mass Spectrometry:

    Article Title: Successful Within-patient Dose Escalation of Olipudase Alfa in Acid Sphingomyelinase Deficiency
    Article Snippet: Pharmacodynamic parameters included measurement of plasma sphingomyelin, and ceramide levels by liquid chromatography-tandem mass spectrometry (LC/MS/MS) and liver sphingomyelin content by both LC/MS/MS and computer-assisted morphometric analysis of hepatic histopathology (MetaMorph Imaging Processing and Analysis software (Version 6.3; Universal Imaging Corporation) ( 11 ).

    Histopathology:

    Article Title: Successful Within-patient Dose Escalation of Olipudase Alfa in Acid Sphingomyelinase Deficiency
    Article Snippet: Pharmacodynamic parameters included measurement of plasma sphingomyelin, and ceramide levels by liquid chromatography-tandem mass spectrometry (LC/MS/MS) and liver sphingomyelin content by both LC/MS/MS and computer-assisted morphometric analysis of hepatic histopathology (MetaMorph Imaging Processing and Analysis software (Version 6.3; Universal Imaging Corporation) ( 11 ).

    Imaging:

    Article Title: Successful Within-patient Dose Escalation of Olipudase Alfa in Acid Sphingomyelinase Deficiency
    Article Snippet: Pharmacodynamic parameters included measurement of plasma sphingomyelin, and ceramide levels by liquid chromatography-tandem mass spectrometry (LC/MS/MS) and liver sphingomyelin content by both LC/MS/MS and computer-assisted morphometric analysis of hepatic histopathology (MetaMorph Imaging Processing and Analysis software (Version 6.3; Universal Imaging Corporation) ( 11 ).

    Software:

    Article Title: Successful Within-patient Dose Escalation of Olipudase Alfa in Acid Sphingomyelinase Deficiency
    Article Snippet: Pharmacodynamic parameters included measurement of plasma sphingomyelin, and ceramide levels by liquid chromatography-tandem mass spectrometry (LC/MS/MS) and liver sphingomyelin content by both LC/MS/MS and computer-assisted morphometric analysis of hepatic histopathology (MetaMorph Imaging Processing and Analysis software (Version 6.3; Universal Imaging Corporation) ( 11 ).



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    Third harmonic generation (THG) brain imaging in groups of Danionella dracula 3 to 90 dpf. A . Left: Side view of a male. Scale bar indicates 3mm. Right: Head-on view of male extending its hypertrophied lower jaw during mid-lunge at another male. B . Dorsal views of fish imaged from 90-3 dpf (left to right). Scale bar left indicates 500 μm for all images. Top insert at 90 dpf is zoom-in on fangs (indicated by arrows; dashed outline on left fang), which are first apparent in 90 dpf males along with hypertrophied lower jaw. C . Schematic of imaging apparatus (see Materials and Methods). Left: profile drawing of animal placement under objective. Right: dorsal view of adult placed in putty holder with perfusion mouthpiece. Fish imaged less than 60 dpf were imaged using an alternative setup than pictured (see Materials and Methods, Fish stabilization for imaging). D. 30, 21, and 14 dpf example fish depicting changes in swim bladder (SB) separation into anterior and posterior chambers. Scale bar indicates 500 μm for all images. E . Schematic of representative D. dracula brain; illustrations based on pictures of dissected whole brains. Brain regions of <t>morphometric</t> analysis indicated in lateral and dorsal views; green, OB (olfactory bulb); blue, telencephalon; orange, TL (torus longitudinalis); pink, TeO (optic tectum). Insert indicates horizontal slice in midbrain depicting TeO (pink), periventricular gray zone (PGZ) of TeO (purple, included in TeO measurement), depicted for indication of boundary between TeO and TS (yellow, torus semicircularis). F . Scatterplot of age in dpf of animals in this study and gross brain volume in μm 3 . Boxplots indicate median, upper and lower quartiles and 1.5× interquartile range for each Age. Gross brain volume calculation is described in and Materials and Methods. Different ages depicted by different colored dots as shown in legend: red, 5 dpf; yellow, 14 dpf; green, 21 dpf; teal, 30 dpf; blue, 60 dpf; purple, 90 dpf. As is described in Materials and Methods, we performed the best fitting quadratic regression (F 2,30= 120.8, R 2 = 0.8821, p<0.001) on log-transformed data to normalize the residuals. The green line displayed here represents the quadratic regression line based on the original (non-transformed) data, for best visual clarity and understanding.
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    Image Search Results


    Third harmonic generation (THG) brain imaging in groups of Danionella dracula 3 to 90 dpf. A . Left: Side view of a male. Scale bar indicates 3mm. Right: Head-on view of male extending its hypertrophied lower jaw during mid-lunge at another male. B . Dorsal views of fish imaged from 90-3 dpf (left to right). Scale bar left indicates 500 μm for all images. Top insert at 90 dpf is zoom-in on fangs (indicated by arrows; dashed outline on left fang), which are first apparent in 90 dpf males along with hypertrophied lower jaw. C . Schematic of imaging apparatus (see Materials and Methods). Left: profile drawing of animal placement under objective. Right: dorsal view of adult placed in putty holder with perfusion mouthpiece. Fish imaged less than 60 dpf were imaged using an alternative setup than pictured (see Materials and Methods, Fish stabilization for imaging). D. 30, 21, and 14 dpf example fish depicting changes in swim bladder (SB) separation into anterior and posterior chambers. Scale bar indicates 500 μm for all images. E . Schematic of representative D. dracula brain; illustrations based on pictures of dissected whole brains. Brain regions of morphometric analysis indicated in lateral and dorsal views; green, OB (olfactory bulb); blue, telencephalon; orange, TL (torus longitudinalis); pink, TeO (optic tectum). Insert indicates horizontal slice in midbrain depicting TeO (pink), periventricular gray zone (PGZ) of TeO (purple, included in TeO measurement), depicted for indication of boundary between TeO and TS (yellow, torus semicircularis). F . Scatterplot of age in dpf of animals in this study and gross brain volume in μm 3 . Boxplots indicate median, upper and lower quartiles and 1.5× interquartile range for each Age. Gross brain volume calculation is described in and Materials and Methods. Different ages depicted by different colored dots as shown in legend: red, 5 dpf; yellow, 14 dpf; green, 21 dpf; teal, 30 dpf; blue, 60 dpf; purple, 90 dpf. As is described in Materials and Methods, we performed the best fitting quadratic regression (F 2,30= 120.8, R 2 = 0.8821, p<0.001) on log-transformed data to normalize the residuals. The green line displayed here represents the quadratic regression line based on the original (non-transformed) data, for best visual clarity and understanding.

    Journal: bioRxiv

    Article Title: Label-free multiphoton imaging reveals volumetric shifts across development in sensory-related brain regions of a miniature transparent vertebrate

    doi: 10.1101/2024.07.18.604134

    Figure Lengend Snippet: Third harmonic generation (THG) brain imaging in groups of Danionella dracula 3 to 90 dpf. A . Left: Side view of a male. Scale bar indicates 3mm. Right: Head-on view of male extending its hypertrophied lower jaw during mid-lunge at another male. B . Dorsal views of fish imaged from 90-3 dpf (left to right). Scale bar left indicates 500 μm for all images. Top insert at 90 dpf is zoom-in on fangs (indicated by arrows; dashed outline on left fang), which are first apparent in 90 dpf males along with hypertrophied lower jaw. C . Schematic of imaging apparatus (see Materials and Methods). Left: profile drawing of animal placement under objective. Right: dorsal view of adult placed in putty holder with perfusion mouthpiece. Fish imaged less than 60 dpf were imaged using an alternative setup than pictured (see Materials and Methods, Fish stabilization for imaging). D. 30, 21, and 14 dpf example fish depicting changes in swim bladder (SB) separation into anterior and posterior chambers. Scale bar indicates 500 μm for all images. E . Schematic of representative D. dracula brain; illustrations based on pictures of dissected whole brains. Brain regions of morphometric analysis indicated in lateral and dorsal views; green, OB (olfactory bulb); blue, telencephalon; orange, TL (torus longitudinalis); pink, TeO (optic tectum). Insert indicates horizontal slice in midbrain depicting TeO (pink), periventricular gray zone (PGZ) of TeO (purple, included in TeO measurement), depicted for indication of boundary between TeO and TS (yellow, torus semicircularis). F . Scatterplot of age in dpf of animals in this study and gross brain volume in μm 3 . Boxplots indicate median, upper and lower quartiles and 1.5× interquartile range for each Age. Gross brain volume calculation is described in and Materials and Methods. Different ages depicted by different colored dots as shown in legend: red, 5 dpf; yellow, 14 dpf; green, 21 dpf; teal, 30 dpf; blue, 60 dpf; purple, 90 dpf. As is described in Materials and Methods, we performed the best fitting quadratic regression (F 2,30= 120.8, R 2 = 0.8821, p<0.001) on log-transformed data to normalize the residuals. The green line displayed here represents the quadratic regression line based on the original (non-transformed) data, for best visual clarity and understanding.

    Article Snippet: Morphometric image analysis was performed using the software Imaris (BITPLANE, Oxford Instruments).

    Techniques: Imaging, Transformation Assay