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image-it 4% formaldehyde fixative solution r37814  (Thermo Fisher)


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    Thermo Fisher image-it 4% formaldehyde fixative solution r37814
    Image It 4% Formaldehyde Fixative Solution R37814, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+it+fixative+solution/image+ittm+fixative+solution/pm40317818-239-6-11
    Average 90 stars, based on 1 article reviews
    image-it 4% formaldehyde fixative solution r37814 - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Calcium (Ca 2+ ) fluxes at mitochondria-ER contact sites (MERCS) are a new target of senolysis in therapy-induced senescence (TIS).
    Article Snippet: Protein quantificationwas performed using the Bradfordmethod in 96-well plates, andOD595 nm was determined using the Infinite 200 PRO plate reader (Tecan).

    Article Title: The IP 3 R inhibitor desmethylxestospongin B reduces tumor cell migration, invasion and metastasis by impairing lysosome acidification and β1-integrin recycling.
    Article Snippet: Cancer is the second leading cause of deathworldwide.>90%of cancer-related deaths are due tometastasis, a process that depends on the ability of cancer cells to leave the primary tumor, migrate, and colonize different tissues.. Inositol 1,4,5-trisphosphate receptor (IP3R)-mediated Ca2+ signaling plays an essential role inmaintaining the homeostasis of cancer cells and the sustained proliferation.. Desmethylxestospongin B (dmXeB) is a specific inhibitor of the IP3R that selectively arrests cell proliferation and promotes cancer cell death at high concentrations.

    Article Title: BRAF Modulates the Interplay Between Cell–Cell and Cell–Extracellular Matrix Adhesions in PECAM-1-Mediated Mechanotransduction
    Article Snippet: Cells grown on Imaging Dish were fixed with Image-iTTM Fixative Solution (ThermoFisher Scientific, Cat# R37814) for 15 min. After that, cells were washed with HBSS, permeabilized (0.25% Triton X-100 in TBS buffer containing 1% Tween (TBS-T), 10 min, room temperature), blocked (1% BSA in TBS-T, 1 h RT), and incubated with the primary antibodies (dilutions prepared in 1% BSA-TBS-T for VE-cadherin—1:400, pMLC—1:200, PECAM-1—1:2000, YAP—1:150 (Cell Signaling) or 1:500 (EMD Millipore Corp.), pFAK—1:150; incubation was performed overnight at 4 °C).

    Article Title: From synthetic vesicles to living cells: Anchoring conducting polymers to cell membrane.
    Article Snippet: Next, cells were treated for 10 min with methanol- free 4% paraformaldehyde (Thermo Fisher Scientific, catalog no. R37814).

    Article Title: Calcium (Ca 2+ ) fluxes at mitochondria-ER contact sites (MERCS) are a new target of senolysis in therapy-induced senescence (TIS)
    Article Snippet: Once the corresponding experimental treatments were completed, cells were fixed with 4% w/v paraformaldehyde (Image-iTTM fixative solution; Thermo Fisher Scientific), washed in PBS, then permeabilized in 0.1% v/v triton X-100 in PBS and blocked for 1 h in 10% w/v BSA in PBS at room temperature.

    Article Title: From synthetic vesicles to living cells: Anchoring conducting polymers to cell membrane
    Article Snippet: Next, cells were treated for 10 min with methanol-free 4% paraformaldehyde (Thermo Fisher Scientific, catalog no. R37814).

    Incubation:

    Article Title: Tissue-specific requirement of Polr1D in the prothoracic gland for ecdysone-mediated developmental transitions in Drosophila melanogaster.
    Article Snippet: Funding information Francis Hendricks Endowment Fund; National Institutes of Health: National Institute of General Medical Sciences, Grant/Award Number: R01-GM141033 Abstract Background: POLR1D is a shared subunit of RNA Polymerases I and III, which transcribe the rRNA incorporated into ribosomes.. Mutations in POLR1D cause Treacher Collins syndrome, a craniofacial disorder that arises from impaired ribosome biogenesis in neural crest cells.. Previously, we found that RNAi knockdown of Polr1D in several non-neural Drosophila tissues caused developmental defects that phenocopy mutations affecting ecdysone signaling.



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen

    doi: 10.1016/j.xpro.2025.103661

    Figure Lengend Snippet:

    Article Snippet: Image-iT fixative solution , Thermo Fisher Scientific , Cat# R37814.

    Techniques: Recombinant, Blocking Assay, Saline, Software, Microscopy