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image-it fixative solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher image-it fixative solution
    Image It Fixative Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+it+fixative+solution/blockaidtm+blocking+solution/pm40022734-48-71-75
    Average 90 stars, based on 1 article reviews
    image-it fixative solution - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Predicting cell cycle stage from 3D single-cell nuclear-stained images.
    Article Snippet: .. Next, cells were blocked with BlockAid Blocking Solution (cat. no. B10710; Thermo Fisher Scientific) containing 0.05% Tween-20 for 30 min at room temperature. .. Cells were then stained with a primary antibody solution overnight at 4C: 3% BlockAid Blocking Solution in 1xPBS with 1:100 concentration of both Predicting cell cycle from 3D single-cell images Li et al. https://doi.org/10.26508/lsa.202403067 vol 8 | no 6 | e202403067 7 of 11 primary goat anti-GFP (cat. No. GPCA-GFP; Encor Biotechnologies) and rabbit anti-RFP (cat. No. 600-401-379; Rockland) antibodies.

    Article Title: Robust and reproducible human intestinal organoid-derived monolayer model for analyzing drug absorption.
    Article Snippet: .. After washing with D-PBS ( −), BlockAid Blocking Solution (B10710; Thermo Fisher Scientific) containing 0.5% Triton X-100 was added and incubated for 1 h at room temperature. .. The monolayer was incubated with primary antibodies [anti-ZO-1 antibody Alexa Fluor 488 (MA3-39,100-A488; Thermo Fisher Scientific), anti-E-cadherin antibody (PA5-32,178; Thermo Fisher Scientific), and anti-MUC2 antibody (NBP225,221; Novus Biologicals)] in blocking solution containing 0.3% Triton X-100 at 4 °C overnight.

    Article Title: Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo.
    Article Snippet: For IHC detection of Cacna1a, sections were first mounted onto slides and subjected to heat-induced epitope retrieval by boiling in 1× citrate buffer, pH 6 (Sigma-Aldrich, C9999) for 10 min in a microwave, followed by thorough washing with 1× PBS. .. For IHC, sections were blocked in BlockAid Blocking Solution (Thermo Fisher Scientific, B10710) with 0.1% Triton X-100 (Sigma-Aldrich, 93443). ..

    Article Title: Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables
    Article Snippet: The intestine integrity and function are crucial for nutrient absorption and to prevent the entry of harmful antigens.. In sub-Saharan Africa, frequent infections and enteric dysfunction can cause intestinal inflammation that is associated with stunting.. Therefore, to study the anti-inflammatory effects of cassava and roselle leaves, commonly consumed as sauces, we developed an in vitro triculture model of intestine inflammation using intestinal Caco-2 (colorectal adenocarcinoma) and HT29-MTX (goblet cell-like) together with the macrophage-like THP-1 cells.

    Article Title: Epigenetic regulation and mechanobiological adaptation in tenocytes during maturation
    Article Snippet: .. Blocking was carried out with BlockAid solution (ThermoFisher, B10710) for 1 h at room temperature to reduce nonspecific binding. .. Cells were incubated overnight at 4 C with rabbit anti-histone H2B antibody (1:50; Proteintech, 15857-1-AP), followed by three PBS washes.

    Article Title: Epigenetic regulation and mechanobiological adaptation in tenocytes during maturation
    Article Snippet: .. Blocking was carried out with BlockAid solution (ThermoFisher, B10710 ) for 1 h at room temperature to reduce nonspecific binding. .. Cells were incubated overnight at 4 °C with rabbit anti-histone H2B antibody (1:50; Proteintech, 15857-1-AP), followed by three PBS washes.

    Incubation:

    Article Title: Robust and reproducible human intestinal organoid-derived monolayer model for analyzing drug absorption.
    Article Snippet: .. After washing with D-PBS ( −), BlockAid Blocking Solution (B10710; Thermo Fisher Scientific) containing 0.5% Triton X-100 was added and incubated for 1 h at room temperature. .. The monolayer was incubated with primary antibodies [anti-ZO-1 antibody Alexa Fluor 488 (MA3-39,100-A488; Thermo Fisher Scientific), anti-E-cadherin antibody (PA5-32,178; Thermo Fisher Scientific), and anti-MUC2 antibody (NBP225,221; Novus Biologicals)] in blocking solution containing 0.3% Triton X-100 at 4 °C overnight.

    Article Title: Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables
    Article Snippet: The intestine integrity and function are crucial for nutrient absorption and to prevent the entry of harmful antigens.. In sub-Saharan Africa, frequent infections and enteric dysfunction can cause intestinal inflammation that is associated with stunting.. Therefore, to study the anti-inflammatory effects of cassava and roselle leaves, commonly consumed as sauces, we developed an in vitro triculture model of intestine inflammation using intestinal Caco-2 (colorectal adenocarcinoma) and HT29-MTX (goblet cell-like) together with the macrophage-like THP-1 cells.

    Article Title: Cntnap2 loss drives striatal neuron hyperexcitability and behavioral inflexibility
    Article Snippet: Neonatal (P1-3) Cntnap2 -/- ;D1-tdT and Cntnap2 +/+ ;D1-tdT mice were cryoanesthetized and injected bilaterally with 200 nL AAV1.hSyn.eGFP.WPRE.bGH (Penn Vector Core, #p1696 ), diluted 1:75 in saline to achieve sparse transduction. .. Brains were post-fixed in 4% PFA in 1 x PBS overnight at 4 ° C. 80 μm coronal sections were made using a freezing microtome (American Optical, AO 860) and stored in 1 x PBS at 4 ° C. Sections were blocked for 1 hr at RT in BlockAid (ThermoFisher, # B10710 ) and incubated for 48 hr with gentle shaking at 4 ° C with antibodies against GFP (1:2500, Abcam, #13970) and RFP (1:1000, Rockland VWR, #600-401-379) diluted in PBS-Tx 1 x PBS with 0.25% Triton X-100 (Sigma, #T8787). .. Sections were washed 3x10 min in PBS-Tx and incubated with gentle shaking for 1 hr at RT with Alexa Fluor 488 and 546 secondary antibodies (1:500, Invitrogen, #A11039, #A11035).

    Immunohistochemistry:

    Article Title: Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo.
    Article Snippet: For IHC detection of Cacna1a, sections were first mounted onto slides and subjected to heat-induced epitope retrieval by boiling in 1× citrate buffer, pH 6 (Sigma-Aldrich, C9999) for 10 min in a microwave, followed by thorough washing with 1× PBS. .. For IHC, sections were blocked in BlockAid Blocking Solution (Thermo Fisher Scientific, B10710) with 0.1% Triton X-100 (Sigma-Aldrich, 93443). ..

    other:

    Article Title: Single-Protein Determinations by Magnetofluorescent Qubit Imaging with Artificial-Intelligence Augmentation at the Point-Of-Care.
    Article Snippet: Get e-Alerts ARTICLE | May 19, 2025 Single-Protein Determinations by Magneto uorescent Qubit Imaging with Arti cial-Intelligence Augmentation at the PointOf-Care , , , , , , , , , and ACS Nano Cite this: ACS Nano 2025, XXXX, XXX, XXX-XXX https://doi.org/10.1021/acsnano.5c04340 © 2025 American Chemical Society Request reuse permissions Cite Share Jump to Yaqi Huang Wei Liu Tiantian Man Jinwei Du Xianli Gong Fujin Lv Wenhao Shan Lu Ding Ying Wan Shengyuan Deng* Open PDF Supporting Information (1) Article Views 110 Altmetric 1 Citations Learn about these metrics Published May 19, 2025 5/25/25, 1:26 PM Single-Protein Determinations by Magnetofluorescent Qubit Imaging with Artificial-Intelligence Augmentation at the Point-Of-Care | ACS... https://pubs.acs.org/doi/10.1021/acsnano.5c04340 2/28 Conventional point-of-care testing (POCT) has limitations in sensitivity with high risks of missed detection or false positive, which restrains its applications for routine outpatient care analysis and early clinical diagnosis.. By merits of the cutting-edge quantum precision metrology, this study devised a mini quantum sensor via magneto uorescent qubit tagging and tunning on core–shelled uorescent nanodiamond FND@SiO .. Comprehensive characterizations con rmed the formation of FND biolabels, while spectroscopies secured no degradation in spin-state transition after surface modi cation.

    Binding Assay:

    Article Title: Epigenetic regulation and mechanobiological adaptation in tenocytes during maturation
    Article Snippet: .. Blocking was carried out with BlockAid solution (ThermoFisher, B10710) for 1 h at room temperature to reduce nonspecific binding. .. Cells were incubated overnight at 4 C with rabbit anti-histone H2B antibody (1:50; Proteintech, 15857-1-AP), followed by three PBS washes.

    Article Title: Epigenetic regulation and mechanobiological adaptation in tenocytes during maturation
    Article Snippet: .. Blocking was carried out with BlockAid solution (ThermoFisher, B10710 ) for 1 h at room temperature to reduce nonspecific binding. .. Cells were incubated overnight at 4 °C with rabbit anti-histone H2B antibody (1:50; Proteintech, 15857-1-AP), followed by three PBS washes.

    Gentle:

    Article Title: Cntnap2 loss drives striatal neuron hyperexcitability and behavioral inflexibility
    Article Snippet: Neonatal (P1-3) Cntnap2 -/- ;D1-tdT and Cntnap2 +/+ ;D1-tdT mice were cryoanesthetized and injected bilaterally with 200 nL AAV1.hSyn.eGFP.WPRE.bGH (Penn Vector Core, #p1696 ), diluted 1:75 in saline to achieve sparse transduction. .. Brains were post-fixed in 4% PFA in 1 x PBS overnight at 4 ° C. 80 μm coronal sections were made using a freezing microtome (American Optical, AO 860) and stored in 1 x PBS at 4 ° C. Sections were blocked for 1 hr at RT in BlockAid (ThermoFisher, # B10710 ) and incubated for 48 hr with gentle shaking at 4 ° C with antibodies against GFP (1:2500, Abcam, #13970) and RFP (1:1000, Rockland VWR, #600-401-379) diluted in PBS-Tx 1 x PBS with 0.25% Triton X-100 (Sigma, #T8787). .. Sections were washed 3x10 min in PBS-Tx and incubated with gentle shaking for 1 hr at RT with Alexa Fluor 488 and 546 secondary antibodies (1:500, Invitrogen, #A11039, #A11035).



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen

    doi: 10.1016/j.xpro.2025.103661

    Figure Lengend Snippet:

    Article Snippet: Image-iT fixative solution , Thermo Fisher Scientific , Cat# R37814.

    Techniques: Recombinant, Blocking Assay, Saline, Software, Microscopy