separate image analysis workflows (Oxford Instruments)
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Oxford Instruments
separate image analysis workflows
Separate Image Analysis Workflows, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44673 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+workflow/Imaris/pmc10824887-77-1-7
Average 99 stars, based on 44673 article reviews
Separate Image Analysis Workflows, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44673 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+workflow/Imaris/pmc10824887-77-1-7
Average 99 stars, based on 44673 article reviews
separate image analysis workflows - by Bioz Stars,
2026-11
99/100 stars
Images
1) Product Images from "Advancements in high-resolution 3D microscopy analysis of endosomal morphology in postmortem Alzheimer’s disease brains"
Article Title: Advancements in high-resolution 3D microscopy analysis of endosomal morphology in postmortem Alzheimer’s disease brains
Journal: Frontiers in Neuroscience
doi: 10.3389/fnins.2023.1321680
Figure Legend Snippet: Overview of Imaris image analysis pipeline: (A) First, parameters are set for neuronal structure identification and segmentation via four separate “object creation” workflows using several representative z-stacks from the AD case series. Two Surface object workflows are created to define the neuronal regions-of-interest (ROIs) in which early endosomes were observed: the MAP2+ somatodendritic neuronal ROI, and neuronal DAPI+ perinuclear ROI. Both Surface object and Spots object workflows are used to gather quantitative data on EEA1+ early endosome puncta, as the Surface and Spots object workflows provide unique statistical variables related to endosome size, morphology, density, and spatial distribution within the neuronal ROIs. The workflows in (A) are then combined into batch pipelines, as shown in (B) to allow for analysis of the entire AD case cohort. First the neuronal ROIs are created using Batch Pipeline 1 (B1), after which the ROIs are refined to exclude disconnected neurites in the neuropil surrounding the neuron imaged from the MAP2+ surface and to remove non-neuronal nuclei from the DAPI+ surface (B2). The EEA1+ green channel is then masked twice over, using the two neuronal ROIs created in steps (B1-B2). Lastly, the endosome analysis batch pipeline (Batch pipeline 2) can be built, comprised of the EEA1+ surface and spots workflows created in (A) , with both workflows duplicated to analyze both the masked EEA1 channels (B4).
Techniques Used:
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