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MetaMorph Inc image software analysis

The effect of SARS-CoV-2 spike protein on LNCaP cell survival. Results with statistically significant ( p < 0.05) differences from the control group are indicated by asterisk (*). A Clonogenic survival assay for LNCaP cells treated with various concentrations of spike protein (SP): 20 ng/mL, 50 ng/mL, 200 ng/mL for 72 h. Control consisted of medium alone for the same timeframe. Colony numbers were quantified as a total percentage of colonies in the control group. B Evaluation of the control group and SP (50 ng/mL) using a cell proliferation kit. Results reflect two independent experiments and are expressed as the average optical density (OD) plus the standard error of the mean (SEM) between control and SP groups. C IHC PCNA staining images displaying relative intensity between the control group (medium only) and the SP group (50 ng/mL), treated for 72 h prior to staining. PCNA+ cells were counted using <t>MetaMorph</t> <t>software.</t> PCNA+ cell counting was performed using a random selection of 5–6 high-power fields from three slides. Images are at original ×400 magnification

Image Software Analysis, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+using+metamorph+software/imaging+system+analysis+software/pmc08775145-106-6-6
Average 90 stars, based on 1 article reviews

image software analysis - by Bioz Stars, 2026-10

90/100 stars

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1) Product Images from "SARS-CoV-2 spike protein inhibits growth of prostate cancer: a potential role of the COVID-19 vaccine killing two birds with one stone"

Article Title: SARS-CoV-2 spike protein inhibits growth of prostate cancer: a potential role of the COVID-19 vaccine killing two birds with one stone

Journal: Medical Oncology (Northwood, London, England)

doi: 10.1007/s12032-021-01628-1

Figure Legend Snippet: The effect of SARS-CoV-2 spike protein on LNCaP cell survival. Results with statistically significant ( p < 0.05) differences from the control group are indicated by asterisk (*). A Clonogenic survival assay for LNCaP cells treated with various concentrations of spike protein (SP): 20 ng/mL, 50 ng/mL, 200 ng/mL for 72 h. Control consisted of medium alone for the same timeframe. Colony numbers were quantified as a total percentage of colonies in the control group. B Evaluation of the control group and SP (50 ng/mL) using a cell proliferation kit. Results reflect two independent experiments and are expressed as the average optical density (OD) plus the standard error of the mean (SEM) between control and SP groups. C IHC PCNA staining images displaying relative intensity between the control group (medium only) and the SP group (50 ng/mL), treated for 72 h prior to staining. PCNA+ cells were counted using MetaMorph software. PCNA+ cell counting was performed using a random selection of 5–6 high-power fields from three slides. Images are at original ×400 magnification

Techniques Used: Control, Clonogenic Cell Survival Assay, Staining, Software, Cell Counting, Selection

The effect of SARS-CoV-2 SP (50 ng/mL) on expression of key pro- and anti- proliferative molecules in LNCaP cells. Statistically significant results are indicated by asterisk (*) ( p < 0.05). A Comparison of mRNA expression levels in pro- and anti-proliferative molecules in LNCaP cells. Pro-proliferative molecules included cyclin B, cyclin D, cyclin E, and CDK4. Anti-proliferative molecules included p18, p21, p27, and p53. GAPDH was used as an internal control for comparison of expression levels. Each experiment was completed in triplicate and recorded on the graphs as a mean ratio of molecule densitometric Units/GAPDH + SEM (× 100). B Evaluation of CDK4 expression in LNCaP cells via IHC staining. IHC images appear as original magnification of 400X. Relative staining intensity was evaluated via MetaMorph image software analysis of 3–5 randomly selected high-power fields of three slides

Figure Legend Snippet: The effect of SARS-CoV-2 SP (50 ng/mL) on expression of key pro- and anti- proliferative molecules in LNCaP cells. Statistically significant results are indicated by asterisk (*) ( p < 0.05). A Comparison of mRNA expression levels in pro- and anti-proliferative molecules in LNCaP cells. Pro-proliferative molecules included cyclin B, cyclin D, cyclin E, and CDK4. Anti-proliferative molecules included p18, p21, p27, and p53. GAPDH was used as an internal control for comparison of expression levels. Each experiment was completed in triplicate and recorded on the graphs as a mean ratio of molecule densitometric Units/GAPDH + SEM (× 100). B Evaluation of CDK4 expression in LNCaP cells via IHC staining. IHC images appear as original magnification of 400X. Relative staining intensity was evaluated via MetaMorph image software analysis of 3–5 randomly selected high-power fields of three slides

Techniques Used: Expressing, Comparison, Control, Immunohistochemistry, Staining, Software

Effect of SARS-CoV-2 SP (50 ng/mL) on LNCaP cell apoptosis. Statistically significant differences in TUNEL + cells and relative caspase-3 activity between the treatment group (SP 50 ng/mL) and control group (medium alone) are indicated with an asterisk (*) ( p < 0.05). A TUNEL staining images and TUNEL + cell percentage comparison between 50 ng/mL SP treatment and control groups. IHC images appear at original ×400 magnification. TUNEL + cells were evaluated using MetaMorph image software on 3–5 randomly selected high-power fields of 3 slides. B Caspase-3 activity of control and SP 50 ng/mL groups of LNCaP cells via caspase-3 activity kit. The experiment was performed in triplicate and results are expressed as the mean caspase-3 activity compared to the controls + SEM

Figure Legend Snippet: Effect of SARS-CoV-2 SP (50 ng/mL) on LNCaP cell apoptosis. Statistically significant differences in TUNEL + cells and relative caspase-3 activity between the treatment group (SP 50 ng/mL) and control group (medium alone) are indicated with an asterisk (*) ( p < 0.05). A TUNEL staining images and TUNEL + cell percentage comparison between 50 ng/mL SP treatment and control groups. IHC images appear at original ×400 magnification. TUNEL + cells were evaluated using MetaMorph image software on 3–5 randomly selected high-power fields of 3 slides. B Caspase-3 activity of control and SP 50 ng/mL groups of LNCaP cells via caspase-3 activity kit. The experiment was performed in triplicate and results are expressed as the mean caspase-3 activity compared to the controls + SEM

Techniques Used: TUNEL Assay, Activity Assay, Control, Staining, Comparison, Software

The effect of SARS-CoV-2 SP (50 ng/mL) on expression of key pro- and anti-apoptotic molecules in LNCaP cells. Statistically significant results are indicated by asterisk (*) ( p < 0.05). A Comparison of mRNA expression levels in pro- and anti-apoptotic molecules in LNCaP cells. Pro-apoptotic molecules included Fas, FasL, TRAILR1, and TRAIL. Anti-apoptotic molecules included FLIP, Bcl-2, and survivin. GAPDH was used as an internal control for comparison of expression levels. Each experiment was completed in triplicate and recorded on the graphs as a mean ratio of molecule densitometric Units/GAPDH + SEM (× 100). B Evaluation of FasL expression in LNCaP cells via IHC staining. The IHC images appear as original ×400 magnification. Relative staining intensity was evaluated via MetaMorph image software analysis of 3–5 randomly selected high-power fields of three slides

Figure Legend Snippet: The effect of SARS-CoV-2 SP (50 ng/mL) on expression of key pro- and anti-apoptotic molecules in LNCaP cells. Statistically significant results are indicated by asterisk (*) ( p < 0.05). A Comparison of mRNA expression levels in pro- and anti-apoptotic molecules in LNCaP cells. Pro-apoptotic molecules included Fas, FasL, TRAILR1, and TRAIL. Anti-apoptotic molecules included FLIP, Bcl-2, and survivin. GAPDH was used as an internal control for comparison of expression levels. Each experiment was completed in triplicate and recorded on the graphs as a mean ratio of molecule densitometric Units/GAPDH + SEM (× 100). B Evaluation of FasL expression in LNCaP cells via IHC staining. The IHC images appear as original ×400 magnification. Relative staining intensity was evaluated via MetaMorph image software analysis of 3–5 randomly selected high-power fields of three slides

Techniques Used: Expressing, Comparison, Control, Immunohistochemistry, Staining, Software

Software:

Article Title: Development and testing of a novel microcantilever technique for measuring the cohesive strength of intact biofilms.
Article Snippet: Cohesive strength is an important parameter for understanding and modeling the mechanics of biomass detachment from bacterial biofilms.. It is challenging to measure the mechanical properties of biofilms, however, because biofilms may desiccate when removed from liquid medium and they are inherently fragile.. Poppele and Hozalski (Poppele and Hozalski, 2003, J Microb Methods 55:607–615) presented a microcantilever method for measuring the tensile strength of detached biofilm fragments while submersed in liquid medium.

Article Title: Glial cell line-derived neurotrophic factor promotes olfactory ensheathing cells migration.
Article Snippet: Olfactory ensheathing cells (OECs) are a unique type of macroglia with axonal growth-promoting properties.. The migrating ability of OECs in CNS is essential for neural regeneration.. However, little is known about the extracellular and intracellular factors that regulate OEC migration.

Article Title: Live-cell imaging of neurofilament transport in cultured neurons
Article Snippet: .. For many years we have used MetaMorph TM image analysis software for most image acquisition, image processing and analysis. ..

Article Title: Live-cell imaging of neurofilament transport in cultured neurons
Article Snippet: .. 7.1 Software For many years we have used MetaMorph TM image analysis software for most image acquisition, image processing and analysis. ..

Article Title: Bone Marrow Stromal Cells Reduce Ischemia-induced Astrocytic Activation In Vitro
Article Snippet: .. Employing the Metamorph image software analysis, a constant positive signal threshold was established and the average signal intensity for each field was quantified and compared using Student’s t-test. .. Short-interfering RNA (siRNA) transfection Mouse IL-6 siRNA was purchased from Qiagene.

Article Title: Exposure to Bleomycin, Etoposide, and Cis -Platinum Alters Rat Sperm Chromatin Integrity and Sperm Head Protein Profile
Article Snippet: .. Therefore, using Metamorph Imaging System analysis software, both the area and perimeter of protamine 1 staining, found within the entire sperm nucleus and thus a comparable indicator of the area and perimeter of the complete sperm head, were assessed. ..

Article Title: SARS-CoV-2 spike protein inhibits growth of prostate cancer: a potential role of the COVID-19 vaccine killing two birds with one stone
Article Snippet: .. Relative staining intensity was evaluated via MetaMorph image software analysis of 3–5 randomly selected high-power fields of three slides ..

Imaging:

Staining:

other:

Article Title: Evaluation of peripheral nerve regeneration via in vivo serial transcutaneous imaging using transgenic Thy1-YFP mice.
Article Snippet: Contents lists available at ScienceDirect Experimental Neurology j ou rna l homepage: www.e lsev ie r.com/ locate /yexnr Evaluation of peripheral nerve regeneration via in vivo serial transcutaneous imaging using transgenic Thy1-YFP mice Ying Yan, Hank H. Sun, Susan E. Mackinnon, Philip J. Johnson ⁎

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