analysis software columbus v 2 5 (Revvity)
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analysis software columbus v 2 5
Analysis Software Columbus V 2 5, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+system/Columbus+Image+Data+Storage+and+Analysis+System/pmc09683089-769-1-5
Average 92 stars, based on 56 article reviews
Analysis Software Columbus V 2 5, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+system/Columbus+Image+Data+Storage+and+Analysis+System/pmc09683089-769-1-5
Average 92 stars, based on 56 article reviews
analysis software columbus v 2 5 - by Bioz Stars,
2026-10
92/100 stars
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Fluorescence:Article Title: Inhibition of an immunometabolic axis of mTORC1 activation extends mammalian healthspan Article Snippet: Each condition was imaged from duplicated wells and a minimum of 7 fields/well using Operetta high-content imaging system 1483 (PerkinElmer). .. The measurement of p16 and Quantitation Assay:Article Title: Chemogenetic profiling of ubiquitin-like modifier pathways identifies NFATC2IP as a mediator of SUMO-dependent genome integrity Article Snippet: Plates were scanned for image acquisitions on an InCell Analyzer 6000 automated microscope (GE Healthcare) with 20x or 60x objectives. .. Image analyses for Software:Article Title: Chemogenetic profiling of ubiquitin-like modifier pathways identifies NFATC2IP as a mediator of SUMO-dependent genome integrity Article Snippet: Plates were scanned for image acquisitions on an InCell Analyzer 6000 automated microscope (GE Healthcare) with 20x or 60x objectives. .. Image analyses for Article Title: The eIF4A2 negative regulator of mRNA translation promotes extracellular matrix deposition to accelerate hepatocellular carcinoma initiation Article Snippet: For fibronectin analysis, cell-derived matrices were blocked with 1% BSA and stained with FN antibody (BD Pharmingen 610078 1:100) for 2h at RT and then with secondary antibody, Alexa-555 (Thermo Fisher 1:400) for 45 min at RT. .. Microscopy:Article Title: Gymnotic uptake of AntimiRs alter microRNA-34a levels in 2D and 3D epithelial cell culture Article Snippet: The plates were sealed with black plate sealers (PerkinElmer, Waltham, MAcat., no. 6050173) and stored at 4°C until analysis. .. The imagining was done using a Article Title: An oxadiazole-based compound potentiates anti-tuberculosis treatment by increasing host resistance via zinc poisoning Article Snippet: After 1 h, cells were washed and stained with HCS CellMask Blue stain (2 μg/mL, Thermo Fisher) and Hoechst 33342 (5 μg/mL, Thermo Fisher) in PBS for 30 minutes at room temperature, washed twice with PBS and stored at 4 °C until acquisition. .. Images were acquired using the automated High Content Screening:Article Title: An oxadiazole-based compound potentiates anti-tuberculosis treatment by increasing host resistance via zinc poisoning Article Snippet: After 1 h, cells were washed and stained with HCS CellMask Blue stain (2 μg/mL, Thermo Fisher) and Hoechst 33342 (5 μg/mL, Thermo Fisher) in PBS for 30 minutes at room temperature, washed twice with PBS and stored at 4 °C until acquisition. .. Images were acquired using the automated Article Title: The eIF4A2 negative regulator of mRNA translation promotes extracellular matrix deposition to accelerate hepatocellular carcinoma initiation Article Snippet: For fibronectin analysis, cell-derived matrices were blocked with 1% BSA and stained with FN antibody (BD Pharmingen 610078 1:100) for 2h at RT and then with secondary antibody, Alexa-555 (Thermo Fisher 1:400) for 45 min at RT. .. other:Article Title: Uptake and anti-inflammatory effects of liposomal astaxanthin on endothelial cells tracked by Raman and fluorescence imaging Article Snippet: ImageJ (National Institutes of Health; http://rsbweb.nih.gov/ij/ ) and Columbus Image Data Storage and Analysis System (Perkin Elmer) were used for the processing of the fluorescence images. Imaging:Article Title: The eIF4A2 negative regulator of mRNA translation promotes extracellular matrix deposition to accelerate hepatocellular carcinoma initiation Article Snippet: For fibronectin analysis, cell-derived matrices were blocked with 1% BSA and stained with FN antibody (BD Pharmingen 610078 1:100) for 2h at RT and then with secondary antibody, Alexa-555 (Thermo Fisher 1:400) for 45 min at RT. .. |
![CLSP-1025 demonstrates functional activity in various cellular systems including cell cocultures and patient-derived organoids. A, CLSP-1025–mediated cytotoxicity in cocultures of PBMCs and HLA-A*02:01 + –p53 R175H+ tumor cells. Cytotoxicity is reported as the percent tumor-cell death after a 96-hour coculture (mean ± SEM; n = 2). B, HLA-A*02:01 surface expression on p53 R175H+ TYK-nu cells after 72 hours of IFN-γ treatment, measured by geometric mean fluorescence intensity (gMFI) via flow cytometry. Data shown as mean; n = 3 technical replicates. C, CLSP-1025–mediated cytotoxicity in PBMC cocultures with TYK-nu cells with or without IFN-γ pretreatment, shown as percent tumor-cell death (mean ± SEM; n = 3 technical replicates). D–E, CLSP-1025–mediated cytotoxicity and IFN-γ secretion in PBMC cocultures with HLA-A*02:01 + –p53 R175H + adenocarcinoma organoids, measured by percent tumor-cell death and MSD, respectively [mean ± standard deviation (SD); n = 1 PBMC donor and technical duplicates for IFN-γ measurements]. F and G, CLSP-1025–mediated cytotoxicity and IFN-γ secretion in PBMC cocultures with HLA-A*02:01 + –p53 WT adenocarcinoma organoids. H, CLSP-1025–mediated killing of GFP + KLE cells when cocultured with isolated CD4 + , CD8 + , or bulk CD3 + T cells. GFP signal was quantified in real time using <t>Incucyte</t> and plotted as normalized fluorescence units [GFP fluorescence units (GFU) × μm 2 /image]. Data shown as mean ± SD; n = 2 technical replicates.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8097/pmc13628097/pmc13628097__ccr-26-1252_f2.jpg)
