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functool 2 image analysis software  (Cytiva Europe)


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    Cytiva Europe functool 2 image analysis software
    Functool 2 Image Analysis Software, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+analysis+computer+software/IN+Carta+image+analysis+software/pmc03753340-63-19-24
    Average 93 stars, based on 41 article reviews
    functool 2 image analysis software - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Software:

    Article Title: Noninvasive Assessment of Response to Neoadjuvant Chemotherapy in Osteosarcoma of Long Bones with Diffusion-Weighted Imaging: An Initial In Vivo Study
    Article Snippet: .. The raw data were transferred to a computer workstation (Sun Microsystems, ADW4.2), where the DWI data was processed using Functool 2 image analysis software (GE Medical Systems, Milwaukee, Wisc., USA) to obtain the pre- and post-treatment tumor apparent diffusion coefficient (ADC) maps and ADC histograms. ..

    Article Title: T-Type Calcium Channel Inhibitors Induce Apoptosis in Medulloblastoma Cells Associated with Altered Metabolic Activity
    Article Snippet: .. Images were analysed using IN Carta Image Analysis Software (GE Healthcare). .. To measure mitochondrial superoxide production, black 96-well plates (655,090, Greiner) were coated with poly-L-lysine (P4832, Sigma-Aldrich, Australia) for 60 min (1:1 in PBS, pH 7.4, 50 μL/well) before 5 × 10 4 cells were seeded in 100-μL media per well and left to adhere for 1 h. Treatments (DMSO control and 3.5, 7.5 and 10 μM of NNC) were added for 6 h before MitoSOX Red (M36008, Thermo Fisher Scientific) at a concentration of 5 μM was added for 30 min. Fluorescence (Ex/Em 510/580 nm) was quantified using a plate reader (Tecan Infinite M Nano, Tecan Austria GmbH).

    Article Title: T-type Calcium Channel Inhibitors Induce Apoptosis in Medulloblastoma Cells Associated With Altered Metabolic Activity
    Article Snippet: Subsequently, cells were washed with PBS and stained with 3 μM calcein-AM (Thermo Fisher Scienti c) and propidium iodide (5 μM) followed by incubation for 30 min. 100 μl of cell suspension was transferred to black plates and imaged with uorescent microscope (IN Cell Analyzer 2000) using excitation of 475 nm /emission of 511 nm for calcein-AM and excitation of 542 nm /emission of 620 nm for PI. .. Generated images were automatically analysed using IN Carta Image Analysis Software (GE Healthcare). .. Assessment of mitochondrial membrane potential Detection of altered mitochondrial membrane potential was performed using 5,5′,6,6′-tetrachloro-1,1′,3,3′tetraethylbenzim-idazolylcarbocyanine iodide (JC-1) Mitochondrial Membrane Potential Assay Kit (ab113850, Abcam) according to the manufacturer’s instructions.

    Article Title: LIN28 expression and function in medulloblastoma
    Article Snippet: Images were recorded by a high-content cell imaging system (IN Cell Analyzer 2200, GE Healthcare Life Sciences) at 20x magni cation. .. Intensity of LIN28B- uorescence for each cell was automatically quanti ed using IN Carta Image Analysis Software (GE Healthcare Life Sciences). ..

    Article Title: Minocycline corrects early, pre-plaque neuroinflammation and inhibits BACE-1 in a transgenic model of Alzheimer's disease-like amyloid pathology
    Article Snippet: The micrographs were taken with a 63× Zeiss plan-Apochromat oil immersion objective in the CA1 area of the hippocampus (eight micrographs per section, three sections per animal for a total of 24 micrographs per animal,) and, as a control, four micrographs were taken in the lateral posterior thalamic nucleus (total of 12 micrographs per animal). .. The images were imported into the MCID 5 Image Analysis Software (Imaging Research Inc.) as tagged image file format files and transformed (with the 'target accent' function) to allow optimal detection by the program. ..


    Article Title: Kynurenic acid, a key L-tryptophan-derived metabolite, protects the heart from an ischemic damage
    Article Snippet: The cells were stained using an automated High Content Imaging pipline (Freedom EVO 200 robot, Tecan Group Ltd., Männedorf, Switzerland) and imaged using the INCell 2200 automated microscope (GE Healthcare, ILL, USA) at X20 magnification (15 fields per well, 30 wells per condition). .. Following image acquisition, high-content image stacks were analyzed using the InCarta software (GE Healthcare) producing comparative fluorescence intensity measurements. .. Cell and nuclei morphology were determined by cytoplasmic and nuclear staining using Calcein Red-Orange (red, 0.30μM; #C34851, Thermo Fisher Scientific, MA, USA) and Hoechst (blue, 1.2μg/ml; #H1399, Thermo Fisher Scientific), respectively.

    Diffusion-based Assay:

    Article Title: Noninvasive Assessment of Response to Neoadjuvant Chemotherapy in Osteosarcoma of Long Bones with Diffusion-Weighted Imaging: An Initial In Vivo Study
    Article Snippet: .. The raw data were transferred to a computer workstation (Sun Microsystems, ADW4.2), where the DWI data was processed using Functool 2 image analysis software (GE Medical Systems, Milwaukee, Wisc., USA) to obtain the pre- and post-treatment tumor apparent diffusion coefficient (ADC) maps and ADC histograms. ..

    Generated:

    Article Title: T-type Calcium Channel Inhibitors Induce Apoptosis in Medulloblastoma Cells Associated With Altered Metabolic Activity
    Article Snippet: Subsequently, cells were washed with PBS and stained with 3 μM calcein-AM (Thermo Fisher Scienti c) and propidium iodide (5 μM) followed by incubation for 30 min. 100 μl of cell suspension was transferred to black plates and imaged with uorescent microscope (IN Cell Analyzer 2000) using excitation of 475 nm /emission of 511 nm for calcein-AM and excitation of 542 nm /emission of 620 nm for PI. .. Generated images were automatically analysed using IN Carta Image Analysis Software (GE Healthcare). .. Assessment of mitochondrial membrane potential Detection of altered mitochondrial membrane potential was performed using 5,5′,6,6′-tetrachloro-1,1′,3,3′tetraethylbenzim-idazolylcarbocyanine iodide (JC-1) Mitochondrial Membrane Potential Assay Kit (ab113850, Abcam) according to the manufacturer’s instructions.

    Transformation Assay:

    Article Title: Minocycline corrects early, pre-plaque neuroinflammation and inhibits BACE-1 in a transgenic model of Alzheimer's disease-like amyloid pathology
    Article Snippet: The micrographs were taken with a 63× Zeiss plan-Apochromat oil immersion objective in the CA1 area of the hippocampus (eight micrographs per section, three sections per animal for a total of 24 micrographs per animal,) and, as a control, four micrographs were taken in the lateral posterior thalamic nucleus (total of 12 micrographs per animal). .. The images were imported into the MCID 5 Image Analysis Software (Imaging Research Inc.) as tagged image file format files and transformed (with the 'target accent' function) to allow optimal detection by the program. ..

    Fluorescence:

    Article Title: Kynurenic acid, a key L-tryptophan-derived metabolite, protects the heart from an ischemic damage
    Article Snippet: The cells were stained using an automated High Content Imaging pipline (Freedom EVO 200 robot, Tecan Group Ltd., Männedorf, Switzerland) and imaged using the INCell 2200 automated microscope (GE Healthcare, ILL, USA) at X20 magnification (15 fields per well, 30 wells per condition). .. Following image acquisition, high-content image stacks were analyzed using the InCarta software (GE Healthcare) producing comparative fluorescence intensity measurements. .. Cell and nuclei morphology were determined by cytoplasmic and nuclear staining using Calcein Red-Orange (red, 0.30μM; #C34851, Thermo Fisher Scientific, MA, USA) and Hoechst (blue, 1.2μg/ml; #H1399, Thermo Fisher Scientific), respectively.



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    Image Search Results


    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Journal: Cells

    Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

    doi: 10.3390/cells15040360

    Figure Lengend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Article Snippet: The average size of metastases was quantified using computer-assisted analysis with CellSens Imaging software (Version 1.17; Olympus, Tokyo, Japan).

    Techniques: Immunodetection, TUNEL Assay