human circrna microarray v2 (Arraystar inc)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Arraystar inc human circrna microarray v2
<t> CircRNA </t> collections on the circRNA microarrays.

Human Circrna Microarray V2, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/illumina+microarray+platform/human+circrna+array+v2/pmc10135611-94-4-3
Average 90 stars, based on 1 article reviews

human circrna microarray v2 - by Bioz Stars, 2026-10

90/100 stars

Images

1) Product Images from "Circular RNA Expression Profiling by Microarray—A Technical and Practical Perspective"

Article Title: Circular RNA Expression Profiling by Microarray—A Technical and Practical Perspective

Journal: Biomolecules

doi: 10.3390/biom13040679

Figure Legend Snippet: CircRNA collections on the circRNA microarrays.

Techniques Used: Microarray, Sequencing, Labeling, Reverse Transcription, Amplification, In Vitro

CircRNA microarray detection of circRNAs. ( A ) The array’s probe sequence spans the circular junction (arrow) of the circRNA. In the circRNA microarray workflow, total RNA ( B ) is treated with RNase R to selectively digest the linear RNAs so as to enrich the circRNAs ( C ). The circRNAs are copied by reverse transcriptase (RT) from the annealed random primers containing a T7 promoter. The cRNA is synthesized from the T7 promoter at the end of the cDNA template by in vitro T7 polymerase transcription, incorporating fluorescent Cy3-UTP ( D ). The fluorescent cRNA is hybridized to the probes printed on the array surface ( E ).

Figure Legend Snippet: CircRNA microarray detection of circRNAs. ( A ) The array’s probe sequence spans the circular junction (arrow) of the circRNA. In the circRNA microarray workflow, total RNA ( B ) is treated with RNase R to selectively digest the linear RNAs so as to enrich the circRNAs ( C ). The circRNAs are copied by reverse transcriptase (RT) from the annealed random primers containing a T7 promoter. The cRNA is synthesized from the T7 promoter at the end of the cDNA template by in vitro T7 polymerase transcription, incorporating fluorescent Cy3-UTP ( D ). The fluorescent cRNA is hybridized to the probes printed on the array surface ( E ).

Techniques Used: Microarray, Sequencing, Reverse Transcription, Synthesized, In Vitro

Figure Legend Snippet: CircRNA probe signal QC flags.

Techniques Used:

Figure Legend Snippet: Snapshot of differentially expressed circRNA table.

Techniques Used:

An example of circular RNA and its binding miRNA. The MRE type (7mer-m8), seed region, 3′ pairing, local AU, and position in the circRNA are displayed.

Figure Legend Snippet: An example of circular RNA and its binding miRNA. The MRE type (7mer-m8), seed region, 3′ pairing, local AU, and position in the circRNA are displayed.

Techniques Used: Binding Assay

Figure Legend Snippet: Biofluid volumes for circRNA microarray.

Techniques Used: Microarray, Isolation, Clinical Proteomics, Cell Culture

Figure Legend Snippet: Published biofluid biomarker studies based on circRNA microarray profiling.

Techniques Used: Biomarker Discovery, Microarray, Clinical Proteomics, Functional Assay, RNA Expression, Diagnostic Assay, Expressing, Virus, Migration, Permeability

In circRNA-qPCR, Primer F and Primer R are situated on the two sides of the circular junction, producing the amplicon that spans the circular junction. The exon numbers are for illustration only.

Figure Legend Snippet: In circRNA-qPCR, Primer F and Primer R are situated on the two sides of the circular junction, producing the amplicon that spans the circular junction. The exon numbers are for illustration only.

Techniques Used: Amplification

Differentially expressed circRNAs from circRNA microarray profiling, analyzed for miRNA sponge functions (published in years 2020–2022).

Figure Legend Snippet: Differentially expressed circRNAs from circRNA microarray profiling, analyzed for miRNA sponge functions (published in years 2020–2022).

Techniques Used: Microarray, Migration

( A ) CircRNA abundance relative to mRNA in total RNA. ( B ) Circular junction reads (in the yellow box region) only represent a small portion of the entire circRNA. The reads from other parts of the circRNA body (arrowed region) are indistinguishable from that of the linear RNA counterparts.

Figure Legend Snippet: ( A ) CircRNA abundance relative to mRNA in total RNA. ( B ) Circular junction reads (in the yellow box region) only represent a small portion of the entire circRNA. The reads from other parts of the circRNA body (arrowed region) are indistinguishable from that of the linear RNA counterparts.

Techniques Used:

Figure Legend Snippet: Low raw counts of a randomly picked circRNA hsa_circ_0054254 in various tissues and cells in the circRNA studies.

Techniques Used:

Density distribution of circRNA counts by circRNA-seq in colorectal cancer and normal control samples (GEO Accession: GSE205241). The limit of quantification (LoQ) is indicated by the blue vertical line and the limit of differential ratio (LoDR, down to a 2-fold change) by the red vertical line. Less than 0.3% of the circRNAs are above the LoDR (shaded red). The circRNA-seq was run on Illumina NovaSeq 6000 at a 150 bp read length and a coverage of 40 million paired end fragments.

Figure Legend Snippet: Density distribution of circRNA counts by circRNA-seq in colorectal cancer and normal control samples (GEO Accession: GSE205241). The limit of quantification (LoQ) is indicated by the blue vertical line and the limit of differential ratio (LoDR, down to a 2-fold change) by the red vertical line. Less than 0.3% of the circRNAs are above the LoDR (shaded red). The circRNA-seq was run on Illumina NovaSeq 6000 at a 150 bp read length and a coverage of 40 million paired end fragments.

Techniques Used: Control

Quantification accuracy (i.e., low error) with RNA abundance by using a microarray or RNA sequencing. Data are based on a large-scale clinical study . While RNA sequencing rapidly loses accuracy (i.e., high error) when the RNA abundance is low, microarray accuracy is relatively unaffected even with a low RNA abundance. Typical circRNA abundance is indicated by the black arrow.

Figure Legend Snippet: Quantification accuracy (i.e., low error) with RNA abundance by using a microarray or RNA sequencing. Data are based on a large-scale clinical study . While RNA sequencing rapidly loses accuracy (i.e., high error) when the RNA abundance is low, microarray accuracy is relatively unaffected even with a low RNA abundance. Typical circRNA abundance is indicated by the black arrow.

Techniques Used: Microarray, RNA Sequencing

Figure Legend Snippet: Differentially expressed circRNAs by circRNA microarray and circRNA-seq.

Techniques Used: Microarray

Figure Legend Snippet: Comparison of the circRNA microarray and circRNA-seq for circRNA expression profiling.

Techniques Used: Comparison, Microarray, Expressing, Sequencing

Labeling:

Article Title: The molecular axis hnRNPU/circKCNK2/EDC4/IL-11 aggravates osteolytic bone metastasis of RCC.
Article Snippet: Using random primers as per the Super RNA Labeling protocol by Arraystar Inc., the total RNA from each specimen was amplified and then converted into fluorescent cRNA (Arraystar Inc.). .. The labeled cRNAs were then hybridized to the Arraystar Human circRNA Array. .. After the washing steps, an Axon GenePix 4000B microarray scanner was used to scan the arrays.

Article Title: Activation of the circAGFG1/miR-195-5p/PD-L1 axis induces lung injury in sepsis.
Article Snippet: The enriched circular RNAs were then amplified and transcribed into fluorescent cRNA using a random priming method provided by the Arraystar Super RNA Labeling Kit. .. The labeled cRNAs were subsequently hybridized onto the Arraystar Human circRNA Array V2 (8 × 15 K, Arraystar). .. Following slide washing, the arrays were scanned using the Agilent Scanner G2505C.

Significance Assay:

Article Title: Interferon-stimulated circHOMER1 attenuates antiviral innate immunity.
Article Snippet: .. Using the human CircRNA Array V2 (8 × 15K, Arraystar) Month XXXX Volume 0 Issue 0 10.1128/mbio.01497-25 2 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m bi o on 1 7 Ju ly 2 02 5 by 2 a0 9: ba c1 :3 4c 0: 18 ::1 f1 :2 11 . with a total of 10,926 circRNA probes, we identified 82 upregulated and 20 downregula ted circRNAs with a threshold of P-value < 0.05 and a fold change cutoff of 2 by volcano plots (Fig. 1A) and heatmap (Fig. 1B). ..

Expressing:

Article Title: Circular RNA TFRC/ SCD1 mRNA interaction regulates ferroptosis and metastasis in gastric cancer
Article Snippet: .. CircRNA expression profiling was performed using the Arraystar Human CircRNA microarray V1 ( GSE93541 , GSE83521 ) and V2 ( GSE194384 ) on GPL19978 and GPL21825 platforms, respectively. ..

Article Title: Circular RNA TFRC/SCD1 mRNA interaction regulates ferroptosis and metastasis in gastric cancer.
Article Snippet: .. CircRNA expression profiling was performed using the Arraystar Human CircRNA microarray V1 (GSE93541, GSE83521) and V2 (GSE194384) on GPL19978 and GPL21825 platforms, respectively. ..

Microarray:

Spectrophotometry:

Article Title: The molecular axis hnRNPU/circKCNK2/EDC4/IL-11 aggravates osteolytic bone metastasis of RCC.
Article Snippet: .. METHODS Arraystar human circRNA array analysis Each sample’s total RNA was measured with a NanoDrop ND-1000 spectrophotometer. .. The guidelines given by Arraystar dictated the steps for sample preparation and microarray hybridization.

other:

Article Title: The molecular axis hnRNPU/circKCNK2/EDC4/IL-11 aggravates osteolytic bone metastasis of RCC.
Article Snippet: RESULTS Increased expression of circKCNK2 was associated with RCC progression and bone metastasis Utilizing the Arraystar human circRNA (V2.0) platform, we performed a differential analysis between five RCC-primary tumors Oncogene and five RCC-bone metastases (Bone Met).

Review

human circrna microarray v2 (Arraystar inc)

Structured Review

Images

1) Product Images from "Circular RNA Expression Profiling by Microarray—A Technical and Practical Perspective"

Related Articles

Labeling:

Significance Assay:

Expressing:

Microarray:

Spectrophotometry:

other:

Similar Products

Image Search Results