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dna microarray method illumina ratref-12 v1  (Illumina Inc)


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    Structured Review

    Illumina Inc dna microarray method illumina ratref-12 v1
    Profiling the gene expression alterations following minocycline i.p. administration to CCI - exposed rats. Gene expression changes 7 days after nerve injury and administration of minocycline (30 mg/kg; i.p .; 16 h and 1 h before CCI and then for 7 days twice daily). The statistical analysis was performed at an ANOVA threshold value of p < 0.001 (N vs . V-CCI vs . MC-CCI). The results of the cDNA <t>microarray</t> analysis are presented in the form of a heat map. The individual columns represent the respective microarrays, while the individual rows represent the regulated genes. The intensity of the color reflects the relative abundance of the transcript and is proportional to the standard deviation from the in-row average, as indicated in the legend below the heat map. (A) The gene pattern represents changes in the abundance levels of 54 mRNA transcripts induced in the lumbar spinal segment on day 7 of neuropathic pain; these abundance levels of these genes were not affected by the addition of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days). (B) Gene pattern B represents changes in the abundance levels of 39 mRNA transcripts induced in the lumbar spinal segment on day 7 of neuropathic pain; the abundance levels of these genes were modulated by repeated intraperitoneal administration of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days). (C) The percentage analysis of the alterations in the CCI-induced gene expression profile in the spinal cord.
    Dna Microarray Method Illumina Ratref 12 V1, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/illumina+microarray+method/ratref+12+expression+beadchip/pmc04131481-47-1-4
    Average 90 stars, based on 1 article reviews
    dna microarray method illumina ratref-12 v1 - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Expression profiling of genes modulated by minocycline in a rat model of neuropathic pain"

    Article Title: Expression profiling of genes modulated by minocycline in a rat model of neuropathic pain

    Journal: Molecular Pain

    doi: 10.1186/1744-8069-10-47

    Profiling the gene expression alterations following minocycline i.p. administration to CCI - exposed rats. Gene expression changes 7 days after nerve injury and administration of minocycline (30 mg/kg; i.p .; 16 h and 1 h before CCI and then for 7 days twice daily). The statistical analysis was performed at an ANOVA threshold value of p < 0.001 (N vs . V-CCI vs . MC-CCI). The results of the cDNA microarray analysis are presented in the form of a heat map. The individual columns represent the respective microarrays, while the individual rows represent the regulated genes. The intensity of the color reflects the relative abundance of the transcript and is proportional to the standard deviation from the in-row average, as indicated in the legend below the heat map. (A) The gene pattern represents changes in the abundance levels of 54 mRNA transcripts induced in the lumbar spinal segment on day 7 of neuropathic pain; these abundance levels of these genes were not affected by the addition of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days). (B) Gene pattern B represents changes in the abundance levels of 39 mRNA transcripts induced in the lumbar spinal segment on day 7 of neuropathic pain; the abundance levels of these genes were modulated by repeated intraperitoneal administration of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days). (C) The percentage analysis of the alterations in the CCI-induced gene expression profile in the spinal cord.
    Figure Legend Snippet: Profiling the gene expression alterations following minocycline i.p. administration to CCI - exposed rats. Gene expression changes 7 days after nerve injury and administration of minocycline (30 mg/kg; i.p .; 16 h and 1 h before CCI and then for 7 days twice daily). The statistical analysis was performed at an ANOVA threshold value of p < 0.001 (N vs . V-CCI vs . MC-CCI). The results of the cDNA microarray analysis are presented in the form of a heat map. The individual columns represent the respective microarrays, while the individual rows represent the regulated genes. The intensity of the color reflects the relative abundance of the transcript and is proportional to the standard deviation from the in-row average, as indicated in the legend below the heat map. (A) The gene pattern represents changes in the abundance levels of 54 mRNA transcripts induced in the lumbar spinal segment on day 7 of neuropathic pain; these abundance levels of these genes were not affected by the addition of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days). (B) Gene pattern B represents changes in the abundance levels of 39 mRNA transcripts induced in the lumbar spinal segment on day 7 of neuropathic pain; the abundance levels of these genes were modulated by repeated intraperitoneal administration of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days). (C) The percentage analysis of the alterations in the CCI-induced gene expression profile in the spinal cord.

    Techniques Used: Gene Expression, Microarray, Standard Deviation

    Genes from the B1 transcription pattern - similarly spinally and DRG regulated by minocycline. Comparison of the CCI-induced changes in transcript levels of genes with expression pattern B1 ( Cd40 , Clec7a and Apobec3b ) in the lumbar spinal cord using microarray (A, D, G) and qPCR (B, E, H) analysis; transcript levels in the DRG (C, F, I) and the modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; i.p. 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) were also examined. The statistical analysis was performed using an ANOVA and Bonferroni’s test. **p < 0.01 and ***p < 0.001 indicate a significant difference when compared to the control (naïve rats); # p < 0.05, ## p < 0.01 and ### p < 0.001 indicate a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).
    Figure Legend Snippet: Genes from the B1 transcription pattern - similarly spinally and DRG regulated by minocycline. Comparison of the CCI-induced changes in transcript levels of genes with expression pattern B1 ( Cd40 , Clec7a and Apobec3b ) in the lumbar spinal cord using microarray (A, D, G) and qPCR (B, E, H) analysis; transcript levels in the DRG (C, F, I) and the modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; i.p. 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) were also examined. The statistical analysis was performed using an ANOVA and Bonferroni’s test. **p < 0.01 and ***p < 0.001 indicate a significant difference when compared to the control (naïve rats); # p < 0.05, ## p < 0.01 and ### p < 0.001 indicate a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).

    Techniques Used: Comparison, Expressing, Microarray, Control, Injection

    Genes from the B1 transcription pattern - differentially spinally and DRG regulated by minocycline. Comparison of the changes in the transcripts levels of genes with expression pattern B1 ( Slc7a7 and Fam22f ) in the lumbar spinal cord using microarray (A, D) and qPCR (B, E) analysis; transcript levels in the DRG (C, F) under neuropathic pain and the modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; i.p. 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) were also examined using qPCR analysis. The statistical analysis was performed using an ANOVA and Bonferroni’s test. ***p < 0.001 indicates a significant difference when compared to the control (naïve rats); # p < 0.05, ## p < 0.01 and ### p < 0.001 indicate a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).
    Figure Legend Snippet: Genes from the B1 transcription pattern - differentially spinally and DRG regulated by minocycline. Comparison of the changes in the transcripts levels of genes with expression pattern B1 ( Slc7a7 and Fam22f ) in the lumbar spinal cord using microarray (A, D) and qPCR (B, E) analysis; transcript levels in the DRG (C, F) under neuropathic pain and the modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; i.p. 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) were also examined using qPCR analysis. The statistical analysis was performed using an ANOVA and Bonferroni’s test. ***p < 0.001 indicates a significant difference when compared to the control (naïve rats); # p < 0.05, ## p < 0.01 and ### p < 0.001 indicate a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).

    Techniques Used: Comparison, Expressing, Microarray, Control, Injection

    Genes from the B2 transcription pattern - similarly spinally and DRG regulated by minocycline. Comparison of the changes in the transcript levels of genes with expression pattern B2 ( Rwdd3 and Gimap5 ) in the lumbar spinal cord using microarray (A, D) and qPCR (B, E) analysis; transcript levels in the DRG (C, F) under neuropathic pain and the modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; i.p. 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) were also examined using qPCR. The statistical analysis was performed using an ANOVA and Bonferroni’s test. **p <0.01 and ***p <0.001 indicate a significant difference when compared to the control (naïve rats); # p < 0.05, ## p < 0.01 and ### p < 0.001 indicate a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).
    Figure Legend Snippet: Genes from the B2 transcription pattern - similarly spinally and DRG regulated by minocycline. Comparison of the changes in the transcript levels of genes with expression pattern B2 ( Rwdd3 and Gimap5 ) in the lumbar spinal cord using microarray (A, D) and qPCR (B, E) analysis; transcript levels in the DRG (C, F) under neuropathic pain and the modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; i.p. 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) were also examined using qPCR. The statistical analysis was performed using an ANOVA and Bonferroni’s test. **p <0.01 and ***p <0.001 indicate a significant difference when compared to the control (naïve rats); # p < 0.05, ## p < 0.01 and ### p < 0.001 indicate a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).

    Techniques Used: Comparison, Expressing, Microarray, Control, Injection

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    Gene Expression:

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    Expressing:

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    RNA Amplification:

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    Hybridization:

    Article Title: Vitamin D Receptor Regulates Liver Regeneration After Partial Hepatectomy in Male Mice.
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    Article Title: Reveal key genes and factors affecting athletes performance in endurance sports using bioinformatic technologies
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    Functional Assay:

    Article Title: Vitamin D Receptor Regulates Liver Regeneration After Partial Hepatectomy in Male Mice.
    Article Snippet: RNA was extracted and prepared for hybridization to Illumina Rat-Ref12 Microarrays.

    Article Title: The role of ACE2 in RAS axis on microglia activation in Parkinson's disease.
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    Article Title: A Gene Expression Biomarker Identifies Chemical Modulators of the Estrogen Receptor α (ERα) in a MCF-7 Microarray Compendium
    Article Snippet: Samples were hybridized onto Human Ref-12 v2.0 Expression BeadChip arrays (Illumina, San Diego, CA) in the National Health and Environmental Effects Laboratory (NHEERL) Genomics Research Core Laboratory using standard Illumina protocols.

    Article Title: Transcriptional development of the hippocampus and the dorsal-intermediate-ventral axis in rats.
    Article Snippet: Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), Trondheim, Norway Bioinformatics Core Facility BioCore, NTNU Norwegian University of Science and Technology, Trondheim, Norway K.G.. Jebsen Center for Genetic Epidemiology, NTNU Norwegian University of Science and Technology, Trondheim, Norway Department of Microbiology, St. Olavs Hospital, Trondheim, Norway Department of Neuroscience and Behavior, Barnard College of Columbia University, New York, New York, USA Kavli Institute for Systems Neuroscience, Egil and Pauline Braathen and Fred Kavli Centre for Cortical Microcircuits, NTNU Norwegian University for Science and Technology, Trondheim, Norway Department of Computer and Information Science, NTNU Norwegian University for Science and Technology, Trondheim, Norway Department of Psychiatry, Columbia University; New York State Psychiatric Institute, New York, New York, USA

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    Article Snippet: 1 Emerging evidence has reported that acute lung injury (ALI), characterized by 2 inflammation and oxidative stress in airway epithelium, is regulated by programmed 3 cell death.. Ferroptosis, a regulated form of cell death spurred by uncontrolled lipid 4 peroxidation, has been proven to implicate various diseases.. Inhibiting ferroptosis 5 represents a feasible strategy for ALI through the suppression of lipid peroxidation, 6 while the mechanism remains to be further elucidated.

    Article Title: Reveal key genes and factors affecting athletes performance in endurance sports using bioinformatic technologies
    Article Snippet: GSE17190 gene expression profiles were obtained using the platform Illumina ratRef-12 v1.0 expression beadchip (Illumina, San Diego, US) [ ] from a public functional genomics data repository GEO database ( https://www.ncbi.nlm.nih.gov/geo ) [ ].

    Article Title: Paracrine signaling of ferroptotic airway epithelium in crystalline silica-induced pulmonary fibrosis augments local fibroblast activation through glycolysis reprogramming.
    Article Snippet: Microarray dataset GSE32147 was downloaded from the National Center of Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) (https://www.ncbi.nlm.nih.gov/geo/) and annotated using the platform GPL6101 Illumina ratRef-12 v1.0 expression beadchip (Edgar et al., 2002).



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    Profiling the gene expression alterations following minocycline i.p. administration to CCI - exposed rats. Gene expression changes 7 days after nerve injury and administration of minocycline (30 mg/kg; i.p .; 16 h and 1 h before CCI and then for 7 days twice daily). The statistical analysis was performed at an ANOVA threshold value of p < 0.001 (N vs . V-CCI vs . MC-CCI). The results of the cDNA microarray analysis are presented in the form of a heat map. The individual columns represent the respective microarrays, while the individual rows represent the regulated genes. The intensity of the color reflects the relative abundance of the transcript and is proportional to the standard deviation from the in-row average, as indicated in the legend below the heat map. (A) The gene pattern represents changes in the abundance levels of 54 mRNA transcripts induced in the lumbar spinal segment on day 7 of neuropathic pain; these abundance levels of these genes were not affected by the addition of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days). (B) Gene pattern B represents changes in the abundance levels of 39 mRNA transcripts induced in the lumbar spinal segment on day 7 of neuropathic pain; the abundance levels of these genes were modulated by repeated intraperitoneal administration of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days). (C) The percentage analysis of the alterations in the CCI-induced gene expression profile in the spinal cord.

    Journal: Molecular Pain

    Article Title: Expression profiling of genes modulated by minocycline in a rat model of neuropathic pain

    doi: 10.1186/1744-8069-10-47

    Figure Lengend Snippet: Profiling the gene expression alterations following minocycline i.p. administration to CCI - exposed rats. Gene expression changes 7 days after nerve injury and administration of minocycline (30 mg/kg; i.p .; 16 h and 1 h before CCI and then for 7 days twice daily). The statistical analysis was performed at an ANOVA threshold value of p < 0.001 (N vs . V-CCI vs . MC-CCI). The results of the cDNA microarray analysis are presented in the form of a heat map. The individual columns represent the respective microarrays, while the individual rows represent the regulated genes. The intensity of the color reflects the relative abundance of the transcript and is proportional to the standard deviation from the in-row average, as indicated in the legend below the heat map. (A) The gene pattern represents changes in the abundance levels of 54 mRNA transcripts induced in the lumbar spinal segment on day 7 of neuropathic pain; these abundance levels of these genes were not affected by the addition of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days). (B) Gene pattern B represents changes in the abundance levels of 39 mRNA transcripts induced in the lumbar spinal segment on day 7 of neuropathic pain; the abundance levels of these genes were modulated by repeated intraperitoneal administration of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days). (C) The percentage analysis of the alterations in the CCI-induced gene expression profile in the spinal cord.

    Article Snippet: The DNA microarray method (Illumina RatRef-12 V1) was used to analyze the changes in mRNA levels in the lumbar segment of the spinal cord.

    Techniques: Gene Expression, Microarray, Standard Deviation

    Genes from the B1 transcription pattern - similarly spinally and DRG regulated by minocycline. Comparison of the CCI-induced changes in transcript levels of genes with expression pattern B1 ( Cd40 , Clec7a and Apobec3b ) in the lumbar spinal cord using microarray (A, D, G) and qPCR (B, E, H) analysis; transcript levels in the DRG (C, F, I) and the modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; i.p. 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) were also examined. The statistical analysis was performed using an ANOVA and Bonferroni’s test. **p < 0.01 and ***p < 0.001 indicate a significant difference when compared to the control (naïve rats); # p < 0.05, ## p < 0.01 and ### p < 0.001 indicate a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).

    Journal: Molecular Pain

    Article Title: Expression profiling of genes modulated by minocycline in a rat model of neuropathic pain

    doi: 10.1186/1744-8069-10-47

    Figure Lengend Snippet: Genes from the B1 transcription pattern - similarly spinally and DRG regulated by minocycline. Comparison of the CCI-induced changes in transcript levels of genes with expression pattern B1 ( Cd40 , Clec7a and Apobec3b ) in the lumbar spinal cord using microarray (A, D, G) and qPCR (B, E, H) analysis; transcript levels in the DRG (C, F, I) and the modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; i.p. 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) were also examined. The statistical analysis was performed using an ANOVA and Bonferroni’s test. **p < 0.01 and ***p < 0.001 indicate a significant difference when compared to the control (naïve rats); # p < 0.05, ## p < 0.01 and ### p < 0.001 indicate a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).

    Article Snippet: The DNA microarray method (Illumina RatRef-12 V1) was used to analyze the changes in mRNA levels in the lumbar segment of the spinal cord.

    Techniques: Comparison, Expressing, Microarray, Control, Injection

    Genes from the B1 transcription pattern - differentially spinally and DRG regulated by minocycline. Comparison of the changes in the transcripts levels of genes with expression pattern B1 ( Slc7a7 and Fam22f ) in the lumbar spinal cord using microarray (A, D) and qPCR (B, E) analysis; transcript levels in the DRG (C, F) under neuropathic pain and the modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; i.p. 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) were also examined using qPCR analysis. The statistical analysis was performed using an ANOVA and Bonferroni’s test. ***p < 0.001 indicates a significant difference when compared to the control (naïve rats); # p < 0.05, ## p < 0.01 and ### p < 0.001 indicate a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).

    Journal: Molecular Pain

    Article Title: Expression profiling of genes modulated by minocycline in a rat model of neuropathic pain

    doi: 10.1186/1744-8069-10-47

    Figure Lengend Snippet: Genes from the B1 transcription pattern - differentially spinally and DRG regulated by minocycline. Comparison of the changes in the transcripts levels of genes with expression pattern B1 ( Slc7a7 and Fam22f ) in the lumbar spinal cord using microarray (A, D) and qPCR (B, E) analysis; transcript levels in the DRG (C, F) under neuropathic pain and the modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; i.p. 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) were also examined using qPCR analysis. The statistical analysis was performed using an ANOVA and Bonferroni’s test. ***p < 0.001 indicates a significant difference when compared to the control (naïve rats); # p < 0.05, ## p < 0.01 and ### p < 0.001 indicate a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).

    Article Snippet: The DNA microarray method (Illumina RatRef-12 V1) was used to analyze the changes in mRNA levels in the lumbar segment of the spinal cord.

    Techniques: Comparison, Expressing, Microarray, Control, Injection

    Genes from the B2 transcription pattern - similarly spinally and DRG regulated by minocycline. Comparison of the changes in the transcript levels of genes with expression pattern B2 ( Rwdd3 and Gimap5 ) in the lumbar spinal cord using microarray (A, D) and qPCR (B, E) analysis; transcript levels in the DRG (C, F) under neuropathic pain and the modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; i.p. 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) were also examined using qPCR. The statistical analysis was performed using an ANOVA and Bonferroni’s test. **p <0.01 and ***p <0.001 indicate a significant difference when compared to the control (naïve rats); # p < 0.05, ## p < 0.01 and ### p < 0.001 indicate a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).

    Journal: Molecular Pain

    Article Title: Expression profiling of genes modulated by minocycline in a rat model of neuropathic pain

    doi: 10.1186/1744-8069-10-47

    Figure Lengend Snippet: Genes from the B2 transcription pattern - similarly spinally and DRG regulated by minocycline. Comparison of the changes in the transcript levels of genes with expression pattern B2 ( Rwdd3 and Gimap5 ) in the lumbar spinal cord using microarray (A, D) and qPCR (B, E) analysis; transcript levels in the DRG (C, F) under neuropathic pain and the modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; i.p. 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) were also examined using qPCR. The statistical analysis was performed using an ANOVA and Bonferroni’s test. **p <0.01 and ***p <0.001 indicate a significant difference when compared to the control (naïve rats); # p < 0.05, ## p < 0.01 and ### p < 0.001 indicate a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).

    Article Snippet: The DNA microarray method (Illumina RatRef-12 V1) was used to analyze the changes in mRNA levels in the lumbar segment of the spinal cord.

    Techniques: Comparison, Expressing, Microarray, Control, Injection