human il 12 (Miltenyi Biotec)
Miltenyi Biotec is a verified supplier 
Miltenyi Biotec manufactures this product 
93
Structured Review
Miltenyi Biotec
human il 12
![NK cell proliferation, biogenesis, and metabolic respiration are heightened during active resolution of neovascular lesions (A) scRNA-seq experimental design for 12- to 16-week-old wild-type male mice treated intraperitoneally (i.p.) with PBS or IL-18 and subjected to laser-induced CNV ( n = 4 mice/group, ≥3 lesions/eye). Scale bar: 50 μm. (B) Isolectin B4 analysis of CNV size in RPE/choroid flatmounts ( n = 11 PBS; n = 17 IL-18 [lesions]). (C) UMAP (Scanpy) of CNV tissue from PBS- and IL-18-treated mice ( n = 8 eyes/condition). Bar chart shows treatment-specific cell-type proportions; see also A. (D) Dot plot of known marker genes across cell types. Dot size indicates proportion of cells in cluster expressing a gene; color indicates relative level of expression. (E) UMAP showing predicted cell-cycle phase with summary bar chart of phase distribution per cell type. (F) Top 20 DEGs in NK cells (IL-18 vs. PBS), ranked by Wilcoxon significance; see also B. (G) Dot plot of NK-related genes in NK1 and NK2 populations under PBS or IL-18 treatment. (H) Ncr1 and CD3ε expression in cells from RPE/choroid of mice ( n = 8 eyes). (I) Pou2f2 , Klrg1, Klrk1 , and Klrb1c gene expression in PBS- versus IL-18-treated mice. (J) Functional enrichment analysis of genes differentially upregulated in NK2 vs. NK1 cells in IL-18-treated samples only; see also A. (K and L) Flow cytometry analysis of CNV tissue from 8- to 10-week-old C57Bl/6J mice treated with PBS or IL-18 ( n = 3 mice/group); see B for gating. (M and N) Flow cytometry of CD69 and IFN-γ expression in splenocytes from naive C57Bl/6J mice (8–10 week old) treated ex vivo with PBS or rIL-18 (three mice per group); see C. (O) Seahorse analysis of energetic phenotype of NK cells isolated from C57Bl/6J mouse splenocytes unstimulated vs. <t>IL-2/IL-12</t> pair vs. IL-18 alone. Bar charts presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01. Following normality testing, unpaired t tests or one-way ANOVA with Dunnett’s multiple comparison tests were used to compare groups.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3971/pmc13293971/pmc13293971__gr4.jpg)
Human Il 12, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+12/Human+IL-12%2C+premium+grade/pmc13293971-72-0-3
Average 93 stars, based on 42 article reviews
Human Il 12, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+12/Human+IL-12%2C+premium+grade/pmc13293971-72-0-3
Average 93 stars, based on 42 article reviews
human il 12 - by Bioz Stars,
2026-11
93/100 stars
Images
1) Product Images from "Circulating natural killer cells are phenotypically and functionally altered in age-related macular degeneration"
Article Title: Circulating natural killer cells are phenotypically and functionally altered in age-related macular degeneration
Journal: Cell Reports Medicine
doi: 10.1016/j.xcrm.2026.102792
Figure Legend Snippet: NK cell proliferation, biogenesis, and metabolic respiration are heightened during active resolution of neovascular lesions (A) scRNA-seq experimental design for 12- to 16-week-old wild-type male mice treated intraperitoneally (i.p.) with PBS or IL-18 and subjected to laser-induced CNV ( n = 4 mice/group, ≥3 lesions/eye). Scale bar: 50 μm. (B) Isolectin B4 analysis of CNV size in RPE/choroid flatmounts ( n = 11 PBS; n = 17 IL-18 [lesions]). (C) UMAP (Scanpy) of CNV tissue from PBS- and IL-18-treated mice ( n = 8 eyes/condition). Bar chart shows treatment-specific cell-type proportions; see also A. (D) Dot plot of known marker genes across cell types. Dot size indicates proportion of cells in cluster expressing a gene; color indicates relative level of expression. (E) UMAP showing predicted cell-cycle phase with summary bar chart of phase distribution per cell type. (F) Top 20 DEGs in NK cells (IL-18 vs. PBS), ranked by Wilcoxon significance; see also B. (G) Dot plot of NK-related genes in NK1 and NK2 populations under PBS or IL-18 treatment. (H) Ncr1 and CD3ε expression in cells from RPE/choroid of mice ( n = 8 eyes). (I) Pou2f2 , Klrg1, Klrk1 , and Klrb1c gene expression in PBS- versus IL-18-treated mice. (J) Functional enrichment analysis of genes differentially upregulated in NK2 vs. NK1 cells in IL-18-treated samples only; see also A. (K and L) Flow cytometry analysis of CNV tissue from 8- to 10-week-old C57Bl/6J mice treated with PBS or IL-18 ( n = 3 mice/group); see B for gating. (M and N) Flow cytometry of CD69 and IFN-γ expression in splenocytes from naive C57Bl/6J mice (8–10 week old) treated ex vivo with PBS or rIL-18 (three mice per group); see C. (O) Seahorse analysis of energetic phenotype of NK cells isolated from C57Bl/6J mouse splenocytes unstimulated vs. IL-2/IL-12 pair vs. IL-18 alone. Bar charts presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01. Following normality testing, unpaired t tests or one-way ANOVA with Dunnett’s multiple comparison tests were used to compare groups.
Techniques Used: Marker, Expressing, Gene Expression, Functional Assay, Flow Cytometry, Ex Vivo, Isolation, Comparison
Figure Legend Snippet: NK cells are found localized adjacent to neovessels in nAMD (A) HRMEC cells were treated for 24 h with 100 μM etoposide and analyzed for expression of markers of cellular senescence ( n = 3). (B) Representative image of β-galactosidase expression in control versus etoposide-treated HRMECs ( n = 3). Scale bar: 200 μm. (C) NKG2DL expression on HRMECs treated with etoposide ( n = 3); see primer list in A. (D–H) NK92MI cells were treated for 18 h with IL-15/18 and incubated with or without HRMECs for 4 h, scale bar: 400 μm, (E) expression of CD107a measured by flow cytometry ( n = 4), (F) cell viability measured by MTS assay ( n = 3), (G) lytic cell death measured by LDH assay ( n = 4), and (H) target cell apoptosis measured by caspase-3/-7 assay ( n = 4). (I) PBMCs were isolated from healthy donors, treated with NK-activating cytokines (IL-12/15 or IL-15/18) for 18 h, and incubated with or without HRMECs for 4 h. Expression of CD107a was measured by flow cytometry ( n = 3–5); see also B. (J and K) Representative and control immunostaining of NCR1 (red) and UEA-1 (green) in cross-sections from non-disease donor controls ( n = 3); see donor information in D. (L) H&E staining in human donor eye with CNV. Orange autofluorescence due to RPE lipofuscin. ∗denotes area of CNV. (M–P) Representative immunostaining of NCR1 (red) and UEA-1 (green) in cross-sections of patients with nAMD ( n = 3); higher magnifications of lesional tissue of one donor shown in Na&b. High-magnification images of two further donors shown in (O) and (M). Closed white arrows denote areas of NCR1 + staining at or adjacent to blood vessels. Open white arrows denote areas of NCR1 + staining in choroidal tissue. Yellow arrows denote areas of NCR1 + staining at the neovascular membrane. Graphical data presented as mean ± SEM. ∗ p < 0.05 ∗∗ p < 0.01, ∗∗∗ p < 0.005, ∗∗∗∗ p < 0.001. Following normality testing, unpaired t tests or one-way ANOVA with Dunnett’s multiple comparison tests were used to compare groups.
Techniques Used: Expressing, Control, Incubation, Flow Cytometry, MTS Assay, Lactate Dehydrogenase Assay, Isolation, Immunostaining, Staining, Membrane, Comparison
Figure Legend Snippet: NK cell immunotherapy reduces neovascular lesion volume and repairs neovessel barrier integrity in models of retinal neovascularization (A) Schematic representation of therapeutic administration of isolated, activated NCR1-GFP − or NCR1-GFP + NK cells in liCNV model in C57Bl/6J mice. (B) RPE/choroid flatmounts from wild-type mice receiving adoptive transfer of IL-12/-15/-18-activated NCR1-GFP − NK cells (top panel) or NCR1-GFP + NK cells were stained for isolectin B4 (red) and DAPI (blue) at 3 days post-liCNV ( n = 4 eyes, 3–4 lesions/eye). Scale bar: 58 μm. (C and D) Schematic representation of therapeutic administration of isolated, activated NK cells in the liCNV model. (D) RPE/choroid flatmounts were stained for isolectin B4 (green) and CNV volume was analyzed by confocal microscopy and IMARIS. n = 5 mice/group, 3 CNVs/eye. Scale bar: 115 μm. (E) Schematic representation of adoptive transfer of isolated, activated C57Bl/6J NK cells in a JR5558 model of spontaneous retinal vascularization ( n = 3–4 mice/group). (E) Fundus fluorescein angiography (FFA) was performed prior to (top), and 5 days following (bottom), NK cell administration. (F) Quantification of leakage area from retinal neovascular lesions. Graphical data presented as mean ± SEM; statistical analysis by unpaired t test or two-way ANOVA with Fisher’s LSD test where ∗ p ≤ 0.05, ∗∗ p < 0.01.
Techniques Used: Isolation, Adoptive Transfer Assay, Staining, Confocal Microscopy
Related Articles
Recombinant:Article Title: SATB1 is a targetable modulator of JAK-STAT signaling and cytokines in human Treg and Tconv cells Article Snippet: Recombinant human IL-2 , Peprotech , Cat#: 200-02. .. Article Title: Age-Related Patterns of Type II Interferon Immunity: Implications for Intramacrophagic Infections and MSMD Diagnosis During Childhood Article Snippet: Panel Surface marker Fluorocrom Company Catalog Number Volume/ Test (μL) T cells TCR αβ FITC BD 333140 2 TCR γδ PE BD 333141 2 CD8 PerCP-Cy5.5 BD 565310 2 CD45RA Pe-Cy7 BD 337186 2 CD45RO APC BD 340438 2 CD4 APC BD 345771 2 CD3 APC-H7 BD 641415 2 HLA-DR V450 BD 655874 2 CCR7 BV510 BD 566760 2 T helper cells CD4 FITC BD 345768 2 CXCR3 PE BD 550633 10 CD8 PerCP-Cy5.5 BD 565310 2 CCR6 Pe-Cy7 BD 560620 2 CD45RO APC BD 340438 2 CD45RA APC-H7 BD 561212 2 CD3 V450 BD 560365 2 CXCR5 BV510 BD 563105 2 IFNγ receptors IFNγR1 PE BD 558937 2 IFNγR2 APC R&D systems FAB773A 10 STAT1 phosphorylation dephosphorilation STAT 1 PE BD 612564 6 CD14 APC BD 555399 6 T cell proliferation assay CD4 FITC BD 345768 2 CD19 Pe-Cy7 BD 557835 2 CD3 APC-H7 BD 641415 2 .. Assay Reagent name Company Catalog number Test* Flow cytometry lysing Lyse solution BD 349202 2mL IL-12/IFN-γ axis culture BCG - - 20 Article Title: Vδ2 T cell activation by malaria is enhanced in second infection via the cell extrinsic cytokine milieu Article Snippet: Sorted naive CD4 + T cells were labelled with 5 μM of CellTrace Violet (1/2500; Biolegend #CD34557A) in PBS for 20 minutes in a 37°C, quenched for 10 minutes in R10 before being used as responder cells in the mixed lymphocyte reaction. .. 5 x 10 4 isolated naive CD4 T cells ( n = 12) were combined in an allogenic mixed lymphocyte reaction with 10 5 isolated γδ T cells ( n = 6) ex vivo , and from media, uRBC or pRBC cultures, and with Human T-Activator CD3/CD28 DynabeadsTM (Gibco, Cat. #11161D) plus recombinant cytokines 1ng/mL Flow Cytometry:Article Title: Age-Related Patterns of Type II Interferon Immunity: Implications for Intramacrophagic Infections and MSMD Diagnosis During Childhood Article Snippet: Panel Surface marker Fluorocrom Company Catalog Number Volume/ Test (μL) T cells TCR αβ FITC BD 333140 2 TCR γδ PE BD 333141 2 CD8 PerCP-Cy5.5 BD 565310 2 CD45RA Pe-Cy7 BD 337186 2 CD45RO APC BD 340438 2 CD4 APC BD 345771 2 CD3 APC-H7 BD 641415 2 HLA-DR V450 BD 655874 2 CCR7 BV510 BD 566760 2 T helper cells CD4 FITC BD 345768 2 CXCR3 PE BD 550633 10 CD8 PerCP-Cy5.5 BD 565310 2 CCR6 Pe-Cy7 BD 560620 2 CD45RO APC BD 340438 2 CD45RA APC-H7 BD 561212 2 CD3 V450 BD 560365 2 CXCR5 BV510 BD 563105 2 IFNγ receptors IFNγR1 PE BD 558937 2 IFNγR2 APC R&D systems FAB773A 10 STAT1 phosphorylation dephosphorilation STAT 1 PE BD 612564 6 CD14 APC BD 555399 6 T cell proliferation assay CD4 FITC BD 345768 2 CD19 Pe-Cy7 BD 557835 2 CD3 APC-H7 BD 641415 2 .. Assay Reagent name Company Catalog number Test* Flow cytometry lysing Lyse solution BD 349202 2mL IL-12/IFN-γ axis culture BCG - - 20 Enzyme-linked Immunosorbent Assay:Article Title: Age-Related Patterns of Type II Interferon Immunity: Implications for Intramacrophagic Infections and MSMD Diagnosis During Childhood Article Snippet: Panel Surface marker Fluorocrom Company Catalog Number Volume/ Test (μL) T cells TCR αβ FITC BD 333140 2 TCR γδ PE BD 333141 2 CD8 PerCP-Cy5.5 BD 565310 2 CD45RA Pe-Cy7 BD 337186 2 CD45RO APC BD 340438 2 CD4 APC BD 345771 2 CD3 APC-H7 BD 641415 2 HLA-DR V450 BD 655874 2 CCR7 BV510 BD 566760 2 T helper cells CD4 FITC BD 345768 2 CXCR3 PE BD 550633 10 CD8 PerCP-Cy5.5 BD 565310 2 CCR6 Pe-Cy7 BD 560620 2 CD45RO APC BD 340438 2 CD45RA APC-H7 BD 561212 2 CD3 V450 BD 560365 2 CXCR5 BV510 BD 563105 2 IFNγ receptors IFNγR1 PE BD 558937 2 IFNγR2 APC R&D systems FAB773A 10 STAT1 phosphorylation dephosphorilation STAT 1 PE BD 612564 6 CD14 APC BD 555399 6 T cell proliferation assay CD4 FITC BD 345768 2 CD19 Pe-Cy7 BD 557835 2 CD3 APC-H7 BD 641415 2 .. Assay Reagent name Company Catalog number Test* Flow cytometry lysing Lyse solution BD 349202 2mL IL-12/IFN-γ axis culture BCG - - 20 Activity Assay:Article Title: Age-Related Patterns of Type II Interferon Immunity: Implications for Intramacrophagic Infections and MSMD Diagnosis During Childhood Article Snippet: Panel Surface marker Fluorocrom Company Catalog Number Volume/ Test (μL) T cells TCR αβ FITC BD 333140 2 TCR γδ PE BD 333141 2 CD8 PerCP-Cy5.5 BD 565310 2 CD45RA Pe-Cy7 BD 337186 2 CD45RO APC BD 340438 2 CD4 APC BD 345771 2 CD3 APC-H7 BD 641415 2 HLA-DR V450 BD 655874 2 CCR7 BV510 BD 566760 2 T helper cells CD4 FITC BD 345768 2 CXCR3 PE BD 550633 10 CD8 PerCP-Cy5.5 BD 565310 2 CCR6 Pe-Cy7 BD 560620 2 CD45RO APC BD 340438 2 CD45RA APC-H7 BD 561212 2 CD3 V450 BD 560365 2 CXCR5 BV510 BD 563105 2 IFNγ receptors IFNγR1 PE BD 558937 2 IFNγR2 APC R&D systems FAB773A 10 STAT1 phosphorylation dephosphorilation STAT 1 PE BD 612564 6 CD14 APC BD 555399 6 T cell proliferation assay CD4 FITC BD 345768 2 CD19 Pe-Cy7 BD 557835 2 CD3 APC-H7 BD 641415 2 .. Assay Reagent name Company Catalog number Test* Flow cytometry lysing Lyse solution BD 349202 2mL IL-12/IFN-γ axis culture BCG - - 20 Concentration Assay:Article Title: Age-Related Patterns of Type II Interferon Immunity: Implications for Intramacrophagic Infections and MSMD Diagnosis During Childhood Article Snippet: Panel Surface marker Fluorocrom Company Catalog Number Volume/ Test (μL) T cells TCR αβ FITC BD 333140 2 TCR γδ PE BD 333141 2 CD8 PerCP-Cy5.5 BD 565310 2 CD45RA Pe-Cy7 BD 337186 2 CD45RO APC BD 340438 2 CD4 APC BD 345771 2 CD3 APC-H7 BD 641415 2 HLA-DR V450 BD 655874 2 CCR7 BV510 BD 566760 2 T helper cells CD4 FITC BD 345768 2 CXCR3 PE BD 550633 10 CD8 PerCP-Cy5.5 BD 565310 2 CCR6 Pe-Cy7 BD 560620 2 CD45RO APC BD 340438 2 CD45RA APC-H7 BD 561212 2 CD3 V450 BD 560365 2 CXCR5 BV510 BD 563105 2 IFNγ receptors IFNγR1 PE BD 558937 2 IFNγR2 APC R&D systems FAB773A 10 STAT1 phosphorylation dephosphorilation STAT 1 PE BD 612564 6 CD14 APC BD 555399 6 T cell proliferation assay CD4 FITC BD 345768 2 CD19 Pe-Cy7 BD 557835 2 CD3 APC-H7 BD 641415 2 .. Assay Reagent name Company Catalog number Test* Flow cytometry lysing Lyse solution BD 349202 2mL IL-12/IFN-γ axis culture BCG - - 20 Isolation:Article Title: Vδ2 T cell activation by malaria is enhanced in second infection via the cell extrinsic cytokine milieu Article Snippet: Sorted naive CD4 + T cells were labelled with 5 μM of CellTrace Violet (1/2500; Biolegend #CD34557A) in PBS for 20 minutes in a 37°C, quenched for 10 minutes in R10 before being used as responder cells in the mixed lymphocyte reaction. .. 5 x 10 4 isolated naive CD4 T cells ( n = 12) were combined in an allogenic mixed lymphocyte reaction with 10 5 isolated γδ T cells ( n = 6) ex vivo , and from media, uRBC or pRBC cultures, and with Human T-Activator CD3/CD28 DynabeadsTM (Gibco, Cat. #11161D) plus recombinant cytokines 1ng/mL Ex Vivo:Article Title: Vδ2 T cell activation by malaria is enhanced in second infection via the cell extrinsic cytokine milieu Article Snippet: Sorted naive CD4 + T cells were labelled with 5 μM of CellTrace Violet (1/2500; Biolegend #CD34557A) in PBS for 20 minutes in a 37°C, quenched for 10 minutes in R10 before being used as responder cells in the mixed lymphocyte reaction. .. 5 x 10 4 isolated naive CD4 T cells ( n = 12) were combined in an allogenic mixed lymphocyte reaction with 10 5 isolated γδ T cells ( n = 6) ex vivo , and from media, uRBC or pRBC cultures, and with Human T-Activator CD3/CD28 DynabeadsTM (Gibco, Cat. #11161D) plus recombinant cytokines 1ng/mL other:Article Title: Circulating natural killer cells are phenotypically and functionally altered in age-related macular degeneration. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Fetal Bovine Serum (FBS) Merck Cat# F9665-500ML Pen-Strep Merck Cat# P4333-100ML OCT mounting media VWR Cat# 361603E MMLV reverse transcriptase Promega Cat# PAM5313 10nm Random Hexamers IDT Cat# 51-01-18-25 RNaseOUTTM Recombinant Ribonuclease Inhibitor Invitrogen Cat# 10777019 RPMI Merck Cat# R8758-500ML Myo-Inositol Sigma Cat# I7508-50G L-Glutamine Sigma Cat# G8540-10MG Folic Acid Sigma Cat# F7876-1G Horse Serum Sigma Cat# H1270-500ML LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation Thermo Fisher Cat# L34957 Proteinase K Thermo Fisher Cat# EO0491 Ethidium Bromide solution Thermo Fisher Cat# 17898 HBSS BioSciences Cat# 14025050 HEPES Buffer Merck Cat# C-40010 DNAse I Merck Cat# 10104159001 Bovine Serum Albumin Merck Cat# 05470 Protease Inhibitor Cocktail Merck Cat# P8340 Triton X-100 Merck Cat# 93422 Endothelial Cell Growth Medium MV2 Kit Merck Cat# C-22121 Lymphoprep Stemcell Technologies Cat# 18061 Hyclone Trypan Blue Thermo Fischer Cat# SV3008401 Murine Recombinant IL-18 GSK/Biotechne Cat# 9139-IL * Human Recombinant IL-18 Biotechne Cat# Multiplex Assay:Article Title: Tumor-Infiltrating Natural Killer Cell Characterization in Pancreatic Ductal Adenocarcinoma Article Snippet: .. Each well contained 1 mL of media (Cell Genix GMP #20801-0500, and multiplex immunofluorescence (mIF) Götingen, Germany) with 10% human serum (Sigma-Aldrich #H4522, Burlington, MA, USA), 1% Penicillin/Streptomycin (Corning #30-002, Tewksbury, MA, USA), 1 μg/mL Clindamycin (Fresenius Kabi #10030860, Bad Homburg vor der Höhe, Germany), 1 μg/mL Amphotericin (Sigma-Aldrich #A2942, Burlington, MA, USA) and 10 μg/mL Ciprofloxacin (Fresenius Kabi #10071960, Bad Homburg vor der Höhe, Germany), as well as IL-2 (1000 IU/mL, Miltenyi #130-097-746, Bergisch Gladbach, Germany), Immunofluorescence:Article Title: Tumor-Infiltrating Natural Killer Cell Characterization in Pancreatic Ductal Adenocarcinoma Article Snippet: .. Each well contained 1 mL of media (Cell Genix GMP #20801-0500, and multiplex immunofluorescence (mIF) Götingen, Germany) with 10% human serum (Sigma-Aldrich #H4522, Burlington, MA, USA), 1% Penicillin/Streptomycin (Corning #30-002, Tewksbury, MA, USA), 1 μg/mL Clindamycin (Fresenius Kabi #10030860, Bad Homburg vor der Höhe, Germany), 1 μg/mL Amphotericin (Sigma-Aldrich #A2942, Burlington, MA, USA) and 10 μg/mL Ciprofloxacin (Fresenius Kabi #10071960, Bad Homburg vor der Höhe, Germany), as well as IL-2 (1000 IU/mL, Miltenyi #130-097-746, Bergisch Gladbach, Germany), |
