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goat anti human igm f ab 2 antibody (SouthernBiotech)

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SouthernBiotech goat anti human igm f ab 2 antibody
Goat Anti Human Igm F Ab 2 Antibody, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igm/Goat+F(ab')2+Anti-Human+IgM-BIOT/us10858364-414-7-12
Average 95 stars, based on 157 article reviews

goat anti human igm f ab 2 antibody - by Bioz Stars, 2026-09

95/100 stars

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Incubation:

Article Title: DC-SIGN Binding to the Surface Ig Oligomannose-type Glycans Promotes Follicular Lymphoma Cell Adhesion and Survival
Article Snippet: .. Cells were resuspended in calcium-enriched medium (RPMI with 1% FBS and 1 mM calcium) and incubated with 20 μg/ml (unless specified) DC-SIGN-Fc (R&D Systems) or soluble polyclonal goat F(ab’) 2 anti-human IgM (Southern Biotech) for 30 minutes on ice in 96-well (U-bottom) plates. ..

Article Title: Stereochemical differences in fluorocyclopropyl amides enable tuning of Btk inhibition and off-target activity
Article Snippet: .. Blood was then stimulated with 50 g/mL of goat anti-IgM F(ab’)2 (Southern Biotech) for 18 h. After incubation, B cells were stained with anti-mouse CD19 PerCP (BD Biosciences, clone SJ25C1), anti-mouse CD27 FITC (BD Biosciences, clone L128) and anti-mouse CD69 PE (BD Biosciences, clone FN50) or Isotype IgG1 PE antibodies for 30 min at room temperature followed by RBC lysis using 1X BD lysis buffer. ..

Mutagenesis:

Article Title: BTK mutation and ibrutinib resistance
Article Snippet: .. 1×106 BTK WT- or mutant-transfected TMD8 cells were treated with 100 nM ibr for 1 hr at 37° C. and followed by stimulation of 5 μg/mL of goat F(ab′)2 anti-human IgM/IgG (Southern Biotech, Birmingham, AL USA) for 15 min. .. Cells were then fixed in 4% formaldehyde for 10 min and permeabilized with 100% methanol for 20 min, and were subjected to staining with Alexa Fluor® 647-anti-phospho-AKT (Ser473), Alexa Fluor® 488 anti-phospho-ERK1/2 (Thr202/Tyr204) (Cell Signaling, Billerica, MA USA), PE-anti-phospho-PLCγ2 (Tyr759), and PE-anti-phospho-BTK (Tyr223) (BD Bioscience, Franklin Lakes, NJ).

Activation Assay:

Article Title: Antibody molecules which bind CD22
Article Snippet: .. After this time B cells were activated by the addition of 200 nM of goat F(ab′)2 anti-human IgM (Southern Biotechnology) for 24 hours at 37 degrees C. To enable detection of cell surface activation markers plates were placed on ice and washed once in ice cold flow buffer (PBS+1% BSA+0.01% NaN3). .. Cells were then stained with a fluorescently labelled anti-CD19 antibody (BD Biosciences) and a fluorescently labelled anti-CD86 antibody and incubated on ice for 1 hour in the dark.

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goat anti human igm f ab 2 antibody (SouthernBiotech)

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